Wuhan aphid virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0QKC6|A0A0P0QKC6_9VIRU Uncharacterized protein OS=Wuhan aphid virus 2 OX=1746068 GN=VP3 PE=4 SV=1
MM1 pKa = 7.71ANLIKK6 pKa = 10.4YY7 pKa = 9.81VIMLRR12 pKa = 11.84VVAAFAGTGMDD23 pKa = 4.76FMEE26 pKa = 5.64KK27 pKa = 10.19DD28 pKa = 3.98GKK30 pKa = 9.28TKK32 pKa = 10.63DD33 pKa = 3.3CAIYY37 pKa = 10.74DD38 pKa = 4.07EE39 pKa = 4.41AWRR42 pKa = 11.84VAKK45 pKa = 10.22CASSLRR51 pKa = 11.84EE52 pKa = 3.71GNMISNKK59 pKa = 9.8LFSFYY64 pKa = 10.9LQVALRR70 pKa = 11.84KK71 pKa = 9.5VPSSSAYY78 pKa = 8.64TSIGFTTGVDD88 pKa = 3.04RR89 pKa = 11.84EE90 pKa = 4.54LIVGKK95 pKa = 9.92QSHH98 pKa = 6.48YY99 pKa = 11.6DD100 pKa = 3.41FDD102 pKa = 3.27QWLYY106 pKa = 9.94TLTYY110 pKa = 10.44DD111 pKa = 4.24PRR113 pKa = 11.84TMLRR117 pKa = 11.84KK118 pKa = 9.02VAVISLHH125 pKa = 6.47RR126 pKa = 11.84EE127 pKa = 3.52YY128 pKa = 10.58TRR130 pKa = 11.84PVLFFSKK137 pKa = 9.38TVPEE141 pKa = 4.6EE142 pKa = 4.0LPVWDD147 pKa = 4.88DD148 pKa = 3.9LMVPSLRR155 pKa = 11.84LAGSGRR161 pKa = 11.84EE162 pKa = 3.73FFIYY166 pKa = 9.89KK167 pKa = 10.12DD168 pKa = 3.54KK169 pKa = 11.05NASPFSPSNAEE180 pKa = 4.02SCSAFYY186 pKa = 10.59DD187 pKa = 3.5WEE189 pKa = 4.16NRR191 pKa = 11.84EE192 pKa = 4.17SFEE195 pKa = 4.52DD196 pKa = 3.62ALGSVMIMDD205 pKa = 4.21PRR207 pKa = 11.84LWCPAMEE214 pKa = 4.68SNRR217 pKa = 11.84FDD219 pKa = 3.4IVVGDD224 pKa = 3.9LEE226 pKa = 4.59EE227 pKa = 4.5IKK229 pKa = 8.25TTSLDD234 pKa = 3.49SKK236 pKa = 10.7LQYY239 pKa = 10.27QSIWEE244 pKa = 4.17VTKK247 pKa = 10.79KK248 pKa = 9.48GTEE251 pKa = 3.86GGRR254 pKa = 11.84LDD256 pKa = 4.05EE257 pKa = 5.07NYY259 pKa = 10.86LSVFF263 pKa = 3.65

Molecular weight: 30.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0QKF2|A0A0P0QKF2_9VIRU Putative glycoprotein OS=Wuhan aphid virus 2 OX=1746068 GN=VP1 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 3.64MTKK5 pKa = 10.66KK6 pKa = 10.62DD7 pKa = 3.41MEE9 pKa = 4.44RR10 pKa = 11.84AQEE13 pKa = 3.76RR14 pKa = 11.84AKK16 pKa = 10.59ILEE19 pKa = 4.14KK20 pKa = 10.58DD21 pKa = 3.8RR22 pKa = 11.84KK23 pKa = 10.03RR24 pKa = 11.84KK25 pKa = 8.87MKK27 pKa = 10.17EE28 pKa = 3.65FQEE31 pKa = 4.25SQKK34 pKa = 10.48MKK36 pKa = 10.49GKK38 pKa = 10.07FKK40 pKa = 10.65GRR42 pKa = 11.84VRR44 pKa = 11.84FWFDD48 pKa = 2.63EE49 pKa = 3.89RR50 pKa = 11.84SYY52 pKa = 11.73VVVRR56 pKa = 11.84DD57 pKa = 3.74ILLSPVFNLVGDD69 pKa = 4.24KK70 pKa = 11.04AHH72 pKa = 6.1MMLVGGYY79 pKa = 9.22LGAVLGVAFLAGRR92 pKa = 11.84IWGWLVPMLYY102 pKa = 9.52MGLIYY107 pKa = 10.5SDD109 pKa = 3.81DD110 pKa = 3.71TNAYY114 pKa = 8.75IAKK117 pKa = 9.71CLLTIWLVSKK127 pKa = 9.71VVKK130 pKa = 10.53CVFKK134 pKa = 10.93KK135 pKa = 10.92KK136 pKa = 10.03EE137 pKa = 4.08DD138 pKa = 4.03TYY140 pKa = 10.9HH141 pKa = 6.2HH142 pKa = 6.73AMMNVYY148 pKa = 10.08HH149 pKa = 7.31KK150 pKa = 9.26PTDD153 pKa = 2.82WMAIGNGIGFYY164 pKa = 10.67VEE166 pKa = 3.51IGIIIGLTYY175 pKa = 10.63LVYY178 pKa = 10.97GDD180 pKa = 4.54GYY182 pKa = 9.7GHH184 pKa = 7.62AFHH187 pKa = 6.66GVWVITNIMILSRR200 pKa = 11.84RR201 pKa = 11.84IPGVGGNSNLGLITLMCLVVIVVALLPEE229 pKa = 4.48VYY231 pKa = 10.22GVLLRR236 pKa = 11.84TTVKK240 pKa = 9.4TLEE243 pKa = 4.29PKK245 pKa = 10.55RR246 pKa = 11.84MAEE249 pKa = 4.04EE250 pKa = 5.14GIGMAAPIGHH260 pKa = 7.56LAGSFSRR267 pKa = 11.84PMDD270 pKa = 3.89YY271 pKa = 10.7IFGLSTTSFLDD282 pKa = 3.36VVRR285 pKa = 11.84ASCGNFLTGWLIFDD299 pKa = 3.87DD300 pKa = 4.03WFGAGHH306 pKa = 7.76ALTTATIIKK315 pKa = 9.29MKK317 pKa = 9.94GKK319 pKa = 9.64EE320 pKa = 3.9VTVPGSAGLYY330 pKa = 9.87GGLGAFWIAGDD341 pKa = 3.78IFLSATSGAVLRR353 pKa = 11.84VIVTVMSIAFSFWMWNWTGSKK374 pKa = 9.15VWSGRR379 pKa = 11.84GKK381 pKa = 10.91GVMLTEE387 pKa = 3.89ARR389 pKa = 11.84ANFGFVFGNGPVGMRR404 pKa = 11.84IAVLRR409 pKa = 11.84ACLFVATMCYY419 pKa = 10.55ALKK422 pKa = 10.55SDD424 pKa = 3.92GNIATGLILMMTITMGSEE442 pKa = 3.46RR443 pKa = 11.84AMTIMLGCMTMHH455 pKa = 7.45LSLFFQGIGKK465 pKa = 9.12NKK467 pKa = 9.93PITEE471 pKa = 4.3SLRR474 pKa = 11.84EE475 pKa = 4.18STVDD479 pKa = 3.27AYY481 pKa = 11.54SHH483 pKa = 7.03FDD485 pKa = 3.44GNASAGTEE493 pKa = 4.02PGG495 pKa = 3.23

Molecular weight: 54.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3062

131

907

510.3

57.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.336 ± 0.59

1.47 ± 0.17

5.389 ± 0.355

5.585 ± 0.752

4.311 ± 0.438

8.165 ± 0.692

2.09 ± 0.257

5.944 ± 0.404

6.434 ± 0.249

9.471 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.625 ± 0.548

3.527 ± 0.291

3.821 ± 0.295

1.96 ± 0.247

5.127 ± 0.502

6.434 ± 0.851

6.434 ± 0.343

7.479 ± 0.294

2.221 ± 0.246

4.18 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski