Hubei picorna-like virus 66

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KJA7|A0A1L3KJA7_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 66 OX=1923149 PE=4 SV=1
MM1 pKa = 8.1SEE3 pKa = 3.65IADD6 pKa = 5.04LIFDD10 pKa = 4.24SNSTLFNVLDD20 pKa = 4.03KK21 pKa = 11.47LDD23 pKa = 3.87VPSLSLEE30 pKa = 3.96VKK32 pKa = 10.07EE33 pKa = 4.65ISEE36 pKa = 4.43SLDD39 pKa = 3.27SSKK42 pKa = 11.13EE43 pKa = 3.85QVRR46 pKa = 11.84QLAAVVDD53 pKa = 4.2NNQVANNTKK62 pKa = 10.0FVDD65 pKa = 3.45VNTRR69 pKa = 11.84LDD71 pKa = 3.91DD72 pKa = 4.61LDD74 pKa = 6.5DD75 pKa = 3.89IVTGTTSDD83 pKa = 3.26AVEE86 pKa = 4.42ALSKK90 pKa = 10.34IDD92 pKa = 3.46TLTTFVRR99 pKa = 11.84EE100 pKa = 3.81NIMRR104 pKa = 11.84IDD106 pKa = 3.74SNIASLEE113 pKa = 3.98RR114 pKa = 11.84TLTTKK119 pKa = 10.36IAALEE124 pKa = 3.94DD125 pKa = 3.83QILKK129 pKa = 9.47TDD131 pKa = 3.77ALANAAFNDD140 pKa = 3.3ISLIGDD146 pKa = 3.32DD147 pKa = 4.11VIRR150 pKa = 11.84VEE152 pKa = 4.5VRR154 pKa = 11.84VNNLDD159 pKa = 3.67EE160 pKa = 4.34EE161 pKa = 4.66SEE163 pKa = 4.13RR164 pKa = 11.84LRR166 pKa = 11.84LDD168 pKa = 3.1VNRR171 pKa = 11.84ANNGVVATQNDD182 pKa = 4.08VAGLTAQVEE191 pKa = 4.72TVQAEE196 pKa = 4.53FSSVPISLEE205 pKa = 3.7EE206 pKa = 4.13IYY208 pKa = 10.7FSTGHH213 pKa = 5.73VLPGVAYY220 pKa = 9.63RR221 pKa = 11.84VVNQAPTNYY230 pKa = 8.27TQRR233 pKa = 11.84TLTLNNPVPYY243 pKa = 10.15RR244 pKa = 11.84SIRR247 pKa = 11.84MGAIYY252 pKa = 10.28SGFIDD257 pKa = 4.28GVLHH261 pKa = 6.42EE262 pKa = 5.83LIQFTDD268 pKa = 4.74AITNTTGGSYY278 pKa = 7.48QTQAGMVPIGGPINWRR294 pKa = 11.84YY295 pKa = 9.89RR296 pKa = 11.84ISAGQPLVSGVIRR309 pKa = 11.84ITKK312 pKa = 9.59QFLRR316 pKa = 11.84MMNPTQTIEE325 pKa = 3.72IHH327 pKa = 7.1DD328 pKa = 4.15MPRR331 pKa = 11.84CVRR334 pKa = 11.84TGFTSLAKK342 pKa = 10.46ALPDD346 pKa = 3.8PSLDD350 pKa = 3.29NGNIISEE357 pKa = 4.26VGNPIVMKK365 pKa = 10.49LPAWGDD371 pKa = 3.31PFLVYY376 pKa = 10.22EE377 pKa = 4.25LRR379 pKa = 11.84KK380 pKa = 9.89KK381 pKa = 10.8RR382 pKa = 11.84NLRR385 pKa = 11.84LPAHH389 pKa = 6.18MFGKK393 pKa = 10.2QKK395 pKa = 10.86LGNTYY400 pKa = 10.59SAFLPRR406 pKa = 11.84IVSAPPIGTHH416 pKa = 6.34VIVDD420 pKa = 4.42PIVTPEE426 pKa = 3.77QADD429 pKa = 4.73LIRR432 pKa = 11.84QYY434 pKa = 11.1RR435 pKa = 11.84AINAHH440 pKa = 6.16VIWIVHH446 pKa = 5.14VPSPIGVGILLEE458 pKa = 4.28VFAPEE463 pKa = 3.92IDD465 pKa = 3.83RR466 pKa = 11.84DD467 pKa = 3.83TKK469 pKa = 10.03TRR471 pKa = 11.84GVRR474 pKa = 11.84FKK476 pKa = 10.7PSGVNTIAFACPWSNDD492 pKa = 3.32LTFVNQDD499 pKa = 2.99SARR502 pKa = 11.84PGQSGGSICIRR513 pKa = 11.84TVEE516 pKa = 4.38DD517 pKa = 3.35NTGEE521 pKa = 4.41TVSTPLKK528 pKa = 8.16ITIYY532 pKa = 9.54QAVVGVNTVGKK543 pKa = 9.76VNRR546 pKa = 11.84GDD548 pKa = 3.56TGVSIPGLNFSPIVIPEE565 pKa = 3.99PPATVIEE572 pKa = 4.93HH573 pKa = 6.99GDD575 pKa = 3.61NDD577 pKa = 4.04PVTTNEE583 pKa = 3.59VNAEE587 pKa = 3.97GGAEE591 pKa = 3.94LVEE594 pKa = 4.19QTTQEE599 pKa = 3.83GAAADD604 pKa = 3.85LVIEE608 pKa = 4.49TEE610 pKa = 4.3KK611 pKa = 10.65PAAPVKK617 pKa = 10.61KK618 pKa = 10.34PEE620 pKa = 3.71ARR622 pKa = 11.84GNNKK626 pKa = 8.5QQATAAARR634 pKa = 11.84WYY636 pKa = 9.95EE637 pKa = 3.82ADD639 pKa = 4.53LVTLNSDD646 pKa = 4.36TLLEE650 pKa = 4.32WKK652 pKa = 10.42NITLDD657 pKa = 3.26PTKK660 pKa = 10.45FSRR663 pKa = 11.84RR664 pKa = 11.84GEE666 pKa = 4.46NISKK670 pKa = 10.58AYY672 pKa = 9.25FRR674 pKa = 11.84NVWFCGHH681 pKa = 5.27MQKK684 pKa = 10.89GYY686 pKa = 9.27ATTLRR691 pKa = 11.84TKK693 pKa = 9.25WVPARR698 pKa = 11.84PPTISGVVEE707 pKa = 4.07IQDD710 pKa = 3.55SRR712 pKa = 11.84NDD714 pKa = 3.09SSRR717 pKa = 11.84YY718 pKa = 8.99LVEE721 pKa = 4.1FGGNVEE727 pKa = 5.02LDD729 pKa = 4.89LIPDD733 pKa = 3.52VHH735 pKa = 7.9ASLTAPARR743 pKa = 11.84PRR745 pKa = 11.84YY746 pKa = 9.23FNNRR750 pKa = 11.84WARR753 pKa = 11.84TTEE756 pKa = 4.03AKK758 pKa = 10.22CQARR762 pKa = 11.84YY763 pKa = 10.06RR764 pKa = 11.84LTGFNRR770 pKa = 11.84TSDD773 pKa = 3.32TADD776 pKa = 2.66IKK778 pKa = 11.27LRR780 pKa = 11.84ILVRR784 pKa = 11.84AGGAIFDD791 pKa = 4.38VPTKK795 pKa = 9.86PRR797 pKa = 11.84PEE799 pKa = 3.95VSPAVAWLVKK809 pKa = 10.18FFEE812 pKa = 5.12DD813 pKa = 2.9WTEE816 pKa = 4.0QKK818 pKa = 10.76DD819 pKa = 4.01LQVLLDD825 pKa = 3.78MSVHH829 pKa = 4.93EE830 pKa = 5.36HH831 pKa = 6.73GLDD834 pKa = 4.84DD835 pKa = 6.62DD836 pKa = 5.01DD837 pKa = 6.99LGDD840 pKa = 4.16GDD842 pKa = 4.55YY843 pKa = 10.45FAPKK847 pKa = 10.25ASNTDD852 pKa = 3.32YY853 pKa = 11.14EE854 pKa = 4.6GEE856 pKa = 4.26INSGEE861 pKa = 4.13ALDD864 pKa = 5.05EE865 pKa = 4.9DD866 pKa = 5.42LEE868 pKa = 4.36QDD870 pKa = 4.02DD871 pKa = 5.03YY872 pKa = 11.65PVQVWEE878 pKa = 4.91GNLPNNGSIVTVPLNLSVAEE898 pKa = 4.38DD899 pKa = 3.67LSGNGGISTIAQKK912 pKa = 9.87FEE914 pKa = 4.09RR915 pKa = 11.84SAHH918 pKa = 5.22IVPTEE923 pKa = 4.17AGDD926 pKa = 3.33MGPRR930 pKa = 11.84LGNYY934 pKa = 9.18VIEE937 pKa = 4.62IRR939 pKa = 11.84LPTNIAGNISHH950 pKa = 6.72VSLPGDD956 pKa = 3.41MSDD959 pKa = 3.56EE960 pKa = 4.07AVALSFGLGDD970 pKa = 3.98ILSMATSALQAVGGPAVSGGIAAGRR995 pKa = 11.84AIFNVVKK1002 pKa = 10.78AIGGKK1007 pKa = 9.33LVNKK1011 pKa = 9.93SKK1013 pKa = 10.86SDD1015 pKa = 3.53SAEE1018 pKa = 3.86QPMLAGPIDD1027 pKa = 3.64VSRR1030 pKa = 11.84FINFIKK1036 pKa = 10.46PIVQNEE1042 pKa = 4.24TLDD1045 pKa = 3.67PTFGSVLMQARR1056 pKa = 11.84DD1057 pKa = 4.3FIGSSGDD1064 pKa = 3.79PITEE1068 pKa = 3.61IPARR1072 pKa = 11.84LWAQMSSRR1080 pKa = 11.84VEE1082 pKa = 3.69RR1083 pKa = 11.84QLFDD1087 pKa = 3.32RR1088 pKa = 11.84TITPIGSLEE1097 pKa = 3.93NEE1099 pKa = 3.87IYY1101 pKa = 10.68LPYY1104 pKa = 10.68DD1105 pKa = 3.57RR1106 pKa = 11.84YY1107 pKa = 11.27SYY1109 pKa = 10.51ILEE1112 pKa = 4.18RR1113 pKa = 11.84FGAHH1117 pKa = 6.69PKK1119 pKa = 9.24TFVEE1123 pKa = 5.02GTHH1126 pKa = 6.7QNVCWKK1132 pKa = 10.58KK1133 pKa = 10.2FLTVLKK1139 pKa = 10.33KK1140 pKa = 10.26KK1141 pKa = 9.77AAKK1144 pKa = 9.63RR1145 pKa = 11.84DD1146 pKa = 3.69VMSLKK1151 pKa = 10.5LSEE1154 pKa = 4.04ILTYY1158 pKa = 10.8EE1159 pKa = 4.25LTDD1162 pKa = 3.78QDD1164 pKa = 4.11EE1165 pKa = 4.0QDD1167 pKa = 3.77LRR1169 pKa = 11.84DD1170 pKa = 4.19IISGKK1175 pKa = 9.13QFVFEE1180 pKa = 4.27PP1181 pKa = 4.05

Molecular weight:
129.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KJA7|A0A1L3KJA7_9VIRU Uncharacterized protein OS=Hubei picorna-like virus 66 OX=1923149 PE=4 SV=1
MM1 pKa = 7.29SVNGKK6 pKa = 8.7FWNADD11 pKa = 3.35DD12 pKa = 4.81SEE14 pKa = 4.92EE15 pKa = 4.24EE16 pKa = 3.95FDD18 pKa = 5.02SRR20 pKa = 11.84GANDD24 pKa = 3.5FEE26 pKa = 4.71EE27 pKa = 5.09EE28 pKa = 3.61FDD30 pKa = 3.9IDD32 pKa = 3.56LFEE35 pKa = 4.38NRR37 pKa = 11.84RR38 pKa = 11.84PKK40 pKa = 10.71LGACDD45 pKa = 3.6EE46 pKa = 4.28APKK49 pKa = 10.82VEE51 pKa = 3.87GKK53 pKa = 10.48VYY55 pKa = 8.01YY56 pKa = 9.56TRR58 pKa = 11.84SKK60 pKa = 9.77TGISALQFRR69 pKa = 11.84KK70 pKa = 9.63QKK72 pKa = 10.51RR73 pKa = 11.84KK74 pKa = 8.1EE75 pKa = 3.81QQEE78 pKa = 4.37TKK80 pKa = 10.5EE81 pKa = 3.89RR82 pKa = 11.84LAKK85 pKa = 9.92QRR87 pKa = 11.84EE88 pKa = 4.17EE89 pKa = 3.72EE90 pKa = 4.06RR91 pKa = 11.84LRR93 pKa = 11.84LLKK96 pKa = 10.7LWGEE100 pKa = 4.05EE101 pKa = 3.93KK102 pKa = 10.4KK103 pKa = 10.93VKK105 pKa = 8.65KK106 pKa = 9.16TKK108 pKa = 10.81ANTPKK113 pKa = 10.66KK114 pKa = 8.15VDD116 pKa = 3.18HH117 pKa = 6.15VARR120 pKa = 11.84SVSNTKK126 pKa = 8.26ITNVSFEE133 pKa = 4.2TKK135 pKa = 10.41LRR137 pKa = 11.84DD138 pKa = 3.48NTEE141 pKa = 3.2IVYY144 pKa = 9.51DD145 pKa = 4.14QRR147 pKa = 11.84ILYY150 pKa = 9.59NADD153 pKa = 3.4SFVEE157 pKa = 4.82GMDD160 pKa = 3.48KK161 pKa = 11.13LKK163 pKa = 10.84VAQHH167 pKa = 6.55LSRR170 pKa = 11.84DD171 pKa = 3.66VSDD174 pKa = 3.6EE175 pKa = 3.79QLPFLIKK182 pKa = 9.72EE183 pKa = 4.14HH184 pKa = 6.85PLAVSKK190 pKa = 10.84PDD192 pKa = 2.98IHH194 pKa = 8.06NLMIYY199 pKa = 10.03GKK201 pKa = 10.07SSLKK205 pKa = 9.15TAIVVEE211 pKa = 4.49AKK213 pKa = 10.0PAEE216 pKa = 4.21KK217 pKa = 10.19RR218 pKa = 11.84SGKK221 pKa = 10.16AINKK225 pKa = 8.6EE226 pKa = 3.74RR227 pKa = 11.84VNLSKK232 pKa = 11.1RR233 pKa = 11.84NFNFGLDD240 pKa = 3.86GKK242 pKa = 9.48PLSSQTNIQDD252 pKa = 3.55TMGKK256 pKa = 9.19KK257 pKa = 10.33KK258 pKa = 10.59NDD260 pKa = 2.95IEE262 pKa = 4.52FPQKK266 pKa = 10.48RR267 pKa = 11.84SVEE270 pKa = 3.98NMTVKK275 pKa = 10.78DD276 pKa = 4.37FLIEE280 pKa = 4.11NNVNSEE286 pKa = 4.12KK287 pKa = 10.39TGSNNNARR295 pKa = 11.84KK296 pKa = 7.39TADD299 pKa = 3.22RR300 pKa = 11.84KK301 pKa = 7.55TEE303 pKa = 3.75KK304 pKa = 10.35RR305 pKa = 11.84ISKK308 pKa = 10.08NKK310 pKa = 8.2RR311 pKa = 11.84EE312 pKa = 3.97KK313 pKa = 10.3MEE315 pKa = 4.2GKK317 pKa = 8.54TEE319 pKa = 3.88IEE321 pKa = 3.85LTIPQSVQTGEE332 pKa = 4.28IEE334 pKa = 4.51LSSEE338 pKa = 4.09LLGSEE343 pKa = 4.07TSVVKK348 pKa = 10.7DD349 pKa = 5.09DD350 pKa = 4.28IPMEE354 pKa = 4.03QTIQTVEE361 pKa = 3.56ITTQTKK367 pKa = 10.13EE368 pKa = 3.22EE369 pKa = 4.02DD370 pKa = 3.62MEE372 pKa = 4.52VKK374 pKa = 10.08TEE376 pKa = 3.98IEE378 pKa = 3.9TAQQVSVEE386 pKa = 4.33TPKK389 pKa = 10.7SDD391 pKa = 3.35EE392 pKa = 4.63AKK394 pKa = 9.79PQPSLLEE401 pKa = 3.86IQISKK406 pKa = 10.41LLEE409 pKa = 4.01EE410 pKa = 4.37VASLNKK416 pKa = 9.0TVQLLLEE423 pKa = 4.71KK424 pKa = 10.01NTALEE429 pKa = 3.95NHH431 pKa = 5.56NTVLAEE437 pKa = 4.45TIANLLEE444 pKa = 4.45NNSKK448 pKa = 10.25EE449 pKa = 4.16DD450 pKa = 3.42KK451 pKa = 10.63KK452 pKa = 11.47LEE454 pKa = 4.19EE455 pKa = 3.75EE456 pKa = 3.89WKK458 pKa = 10.75AIEE461 pKa = 4.3KK462 pKa = 9.31EE463 pKa = 4.09KK464 pKa = 11.09KK465 pKa = 10.31SIEE468 pKa = 3.98EE469 pKa = 3.87EE470 pKa = 3.79KK471 pKa = 10.86RR472 pKa = 11.84SIEE475 pKa = 4.01EE476 pKa = 3.62QKK478 pKa = 11.23LKK480 pKa = 10.47IEE482 pKa = 4.14PSKK485 pKa = 10.74EE486 pKa = 3.74PSKK489 pKa = 11.36APILFEE495 pKa = 4.53KK496 pKa = 10.51LATRR500 pKa = 11.84IKK502 pKa = 9.51GTPGEE507 pKa = 4.32KK508 pKa = 9.82KK509 pKa = 10.46SPKK512 pKa = 9.83NPNAEE517 pKa = 3.85ARR519 pKa = 11.84EE520 pKa = 3.93KK521 pKa = 10.86RR522 pKa = 11.84RR523 pKa = 11.84IPFVNNGASKK533 pKa = 10.5DD534 pKa = 3.87VPVKK538 pKa = 10.36KK539 pKa = 10.01QEE541 pKa = 3.85KK542 pKa = 7.77TVEE545 pKa = 4.19KK546 pKa = 10.63KK547 pKa = 10.59EE548 pKa = 4.03EE549 pKa = 4.02PKK551 pKa = 10.49QEE553 pKa = 3.88IEE555 pKa = 4.24LVEE558 pKa = 4.36EE559 pKa = 4.42PTTSKK564 pKa = 9.99TPDD567 pKa = 3.13SPKK570 pKa = 9.64NTGAIPKK577 pKa = 9.65IPKK580 pKa = 9.19EE581 pKa = 4.13EE582 pKa = 4.05KK583 pKa = 10.56AEE585 pKa = 3.97EE586 pKa = 4.12LVSKK590 pKa = 9.31FTDD593 pKa = 3.57DD594 pKa = 4.41GNTLKK599 pKa = 11.13VNLDD603 pKa = 3.68LAQTEE608 pKa = 4.4EE609 pKa = 4.19EE610 pKa = 3.96QRR612 pKa = 11.84EE613 pKa = 4.33VIGNIILNDD622 pKa = 3.48EE623 pKa = 4.26EE624 pKa = 4.46TLKK627 pKa = 11.05KK628 pKa = 10.47AVNQGVRR635 pKa = 11.84PVNTKK640 pKa = 8.05TVQASFAEE648 pKa = 4.42EE649 pKa = 3.84LAKK652 pKa = 10.96GLVRR656 pKa = 11.84SKK658 pKa = 11.27SFVHH662 pKa = 5.8YY663 pKa = 10.3SPEE666 pKa = 3.41KK667 pKa = 9.1RR668 pKa = 11.84AVIKK672 pKa = 10.5RR673 pKa = 11.84DD674 pKa = 3.37PNKK677 pKa = 10.38PKK679 pKa = 10.68GIYY682 pKa = 8.31WDD684 pKa = 3.12DD685 pKa = 3.3WEE687 pKa = 5.07RR688 pKa = 11.84IKK690 pKa = 11.07LKK692 pKa = 10.63PKK694 pKa = 10.09DD695 pKa = 3.69VQEE698 pKa = 4.06RR699 pKa = 11.84AVRR702 pKa = 11.84KK703 pKa = 9.61AYY705 pKa = 10.74NSLFYY710 pKa = 10.81GLCTRR715 pKa = 11.84LRR717 pKa = 11.84QRR719 pKa = 11.84WEE721 pKa = 3.69IVSPEE726 pKa = 4.14KK727 pKa = 10.37PNPYY731 pKa = 8.37LTKK734 pKa = 10.45PSFIWNRR741 pKa = 11.84LATKK745 pKa = 10.31SIGDD749 pKa = 3.12IWSCMEE755 pKa = 3.61VWRR758 pKa = 11.84KK759 pKa = 8.39GACSYY764 pKa = 10.65KK765 pKa = 10.44VAGIPEE771 pKa = 4.05NWNKK775 pKa = 10.62LEE777 pKa = 3.95

Molecular weight:
88.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3903

777

1945

1301.0

146.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.175 ± 0.637

1.409 ± 0.555

6.226 ± 0.507

8.045 ± 1.529

3.818 ± 0.466

5.765 ± 0.696

1.922 ± 0.516

6.713 ± 0.321

7.533 ± 2.015

8.583 ± 0.514

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.793 ± 0.272

5.406 ± 0.315

4.202 ± 0.759

3.484 ± 0.161

5.047 ± 0.204

6.226 ± 0.229

6.149 ± 0.406

7.584 ± 0.481

1.537 ± 0.192

2.383 ± 0.357

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski