Arthrobacter glacialis
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3921 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S3ZW25|A0A2S3ZW25_ARTGL Dipeptidase OS=Arthrobacter glacialis OX=1664 GN=CVS27_10950 PE=4 SV=1
MM1 pKa = 7.73 SDD3 pKa = 2.82 SSTPAGVIVCQDD15 pKa = 2.5 SDD17 pKa = 4.21 VQVKK21 pKa = 10.51 SSLLVEE27 pKa = 4.44 VDD29 pKa = 4.33 DD30 pKa = 4.61 FPIAIVRR37 pKa = 11.84 DD38 pKa = 3.99 SYY40 pKa = 12.14 GDD42 pKa = 3.09 LHH44 pKa = 8.12 AIGDD48 pKa = 4.28 TCSHH52 pKa = 6.63 AEE54 pKa = 3.68 ISLSEE59 pKa = 4.11 GDD61 pKa = 4.18 VEE63 pKa = 6.36 DD64 pKa = 4.25 GTIEE68 pKa = 4.13 CWAHH72 pKa = 6.22 GSSFDD77 pKa = 4.14 LKK79 pKa = 10.85 SGEE82 pKa = 4.59 PLTLPAFEE90 pKa = 4.63 PVPVFALSIHH100 pKa = 6.13 GSDD103 pKa = 3.87 VYY105 pKa = 11.81 VDD107 pKa = 3.2 VTNILNGVSAQQ118 pKa = 3.36
Molecular weight: 12.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A2S4A1A0|A0A2S4A1A0_ARTGL Thioredoxin domain-containing protein OS=Arthrobacter glacialis OX=1664 GN=CVS27_00180 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.44 KK7 pKa = 9.48 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.32 RR11 pKa = 11.84 MSKK14 pKa = 9.94 KK15 pKa = 9.5 KK16 pKa = 9.79 HH17 pKa = 5.45 RR18 pKa = 11.84 KK19 pKa = 6.15 QLRR22 pKa = 11.84 KK23 pKa = 7.92 TRR25 pKa = 11.84 HH26 pKa = 3.43 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3921
0
3921
1276308
28
3473
325.5
34.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.077 ± 0.051
0.61 ± 0.01
5.341 ± 0.028
5.268 ± 0.037
3.289 ± 0.023
8.79 ± 0.032
2.121 ± 0.02
4.6 ± 0.03
2.997 ± 0.029
10.268 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.202 ± 0.019
2.83 ± 0.025
5.27 ± 0.031
3.264 ± 0.02
5.741 ± 0.032
6.208 ± 0.026
6.244 ± 0.044
8.38 ± 0.031
1.456 ± 0.016
2.044 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here