Pelagicola sp. LXJ1103
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2960 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2CZI8|A0A2U2CZI8_9RHOB MarR family transcriptional regulator OS=Pelagicola sp. LXJ1103 OX=2175247 GN=DD563_06370 PE=4 SV=1
MM1 pKa = 7.7 AAPATAQEE9 pKa = 4.2 WDD11 pKa = 4.24 LNWGGFMNQHH21 pKa = 6.03 IAFADD26 pKa = 3.64 VSGTTVTTSANDD38 pKa = 4.64 GIVFLSTTEE47 pKa = 4.28 IIFTPSVTLDD57 pKa = 2.91 NGLTFGVNVQMEE69 pKa = 4.57 GEE71 pKa = 4.27 NNGGGAIGIDD81 pKa = 3.84 EE82 pKa = 4.96 TYY84 pKa = 10.44 MSISGDD90 pKa = 3.15 AFGQLDD96 pKa = 3.45 IGSEE100 pKa = 3.78 NSAGYY105 pKa = 9.83 KK106 pKa = 9.42 MSVAAPGVTSMFINSPSISAFIPLSSALPWNFRR139 pKa = 11.84 QAGVSAYY146 pKa = 9.84 TEE148 pKa = 3.99 VAGNNDD154 pKa = 3.48 VDD156 pKa = 3.66 RR157 pKa = 11.84 LTYY160 pKa = 7.45 YY161 pKa = 10.1 TPSFNGLTLGVSYY174 pKa = 11.16 ARR176 pKa = 11.84 NNQGNAVSSSNANAAGSVEE195 pKa = 4.9 DD196 pKa = 4.0 IFDD199 pKa = 3.39 IGVAYY204 pKa = 10.48 SQTFGTTSVDD214 pKa = 3.15 LSARR218 pKa = 11.84 FGTGDD223 pKa = 3.46 RR224 pKa = 11.84 TALPAAAAVAATPGNAGTAAVTAVPAGTVEE254 pKa = 4.14 TWAVGAQFGFGDD266 pKa = 4.3 FAVGGHH272 pKa = 5.24 YY273 pKa = 10.84 AEE275 pKa = 4.95 NDD277 pKa = 3.22 AGLVGDD283 pKa = 5.35 NGDD286 pKa = 3.42 QSSWGLGGTYY296 pKa = 11.08 DD297 pKa = 4.35 MAGPWAFEE305 pKa = 3.38 AVMFFGEE312 pKa = 4.58 FEE314 pKa = 4.22 NSGTAGADD322 pKa = 3.35 EE323 pKa = 5.21 EE324 pKa = 4.88 YY325 pKa = 10.43 NAYY328 pKa = 10.47 RR329 pKa = 11.84 IGASRR334 pKa = 11.84 DD335 pKa = 3.37 LGPGVDD341 pKa = 3.04 WDD343 pKa = 3.65 IYY345 pKa = 11.16 AVYY348 pKa = 10.84 AEE350 pKa = 5.07 ADD352 pKa = 3.63 DD353 pKa = 4.29 KK354 pKa = 11.76 GAVGTEE360 pKa = 3.94 VKK362 pKa = 9.76 GTVIGTAINLSFF374 pKa = 4.58
Molecular weight: 38.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.376
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.024
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.808
Patrickios 0.807
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A2U2CVK3|A0A2U2CVK3_9RHOB ATP synthase subunit a OS=Pelagicola sp. LXJ1103 OX=2175247 GN=atpB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.02 GRR39 pKa = 11.84 KK40 pKa = 8.91 SLSAA44 pKa = 3.86
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2960
0
2960
927427
28
2120
313.3
33.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.715 ± 0.053
0.932 ± 0.015
5.952 ± 0.036
5.934 ± 0.036
3.656 ± 0.03
8.833 ± 0.043
2.122 ± 0.024
5.274 ± 0.032
3.109 ± 0.033
9.901 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.022 ± 0.023
2.474 ± 0.022
5.113 ± 0.029
3.117 ± 0.025
6.649 ± 0.043
5.021 ± 0.027
5.48 ± 0.029
7.077 ± 0.039
1.382 ± 0.02
2.237 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here