Methylocystis heyeri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylocystis

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B8KHG9|A0A6B8KHG9_9RHIZ Glutathione S-transferase family protein OS=Methylocystis heyeri OX=391905 GN=H2LOC_015935 PE=3 SV=1
MM1 pKa = 7.53SFNIFLINFHH11 pKa = 6.57NGEE14 pKa = 4.24PFPFPFSLFQKK25 pKa = 10.67SFGSYY30 pKa = 10.52CSDD33 pKa = 3.1QTGDD37 pKa = 3.61CWRR40 pKa = 11.84LDD42 pKa = 3.69YY43 pKa = 10.96PDD45 pKa = 5.68GGWCDD50 pKa = 3.3IDD52 pKa = 4.21GEE54 pKa = 4.32PMDD57 pKa = 4.53GFGVSS62 pKa = 3.24

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B8KHP0|A0A6B8KHP0_9RHIZ Hopanoid biosynthesis-associated protein HpnK OS=Methylocystis heyeri OX=391905 GN=hpnK PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.36VIATRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.01RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4086

0

4086

1319365

23

8978

322.9

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.097 ± 0.066

0.98 ± 0.014

5.354 ± 0.027

6.041 ± 0.045

3.996 ± 0.027

8.467 ± 0.04

1.95 ± 0.018

4.823 ± 0.024

3.477 ± 0.032

10.122 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.016

2.7 ± 0.029

5.438 ± 0.032

3.066 ± 0.025

7.321 ± 0.037

5.86 ± 0.03

4.676 ± 0.034

6.872 ± 0.03

1.318 ± 0.016

2.376 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski