Amino acid dipepetide frequency for Anopheles gambiae (African malaria mosquito)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.008AlaAla: 9.008 ± 0.08	1.179AlaCys: 1.179 ± 0.015	3.649AlaAsp: 3.649 ± 0.024	4.431AlaGlu: 4.431 ± 0.034	2.287AlaPhe: 2.287 ± 0.018	5.831AlaGly: 5.831 ± 0.041	1.673AlaHis: 1.673 ± 0.015	3.619AlaIle: 3.619 ± 0.024	3.954AlaLys: 3.954 ± 0.036	6.439AlaLeu: 6.439 ± 0.037	1.71AlaMet: 1.71 ± 0.012	3.249AlaAsn: 3.249 ± 0.023	4.025AlaPro: 4.025 ± 0.032	3.009AlaGln: 3.009 ± 0.021	3.603AlaArg: 3.603 ± 0.024	6.37AlaSer: 6.37 ± 0.038	5.099AlaThr: 5.099 ± 0.032	4.86AlaVal: 4.86 ± 0.027	0.578AlaTrp: 0.578 ± 0.009	1.835AlaTyr: 1.835 ± 0.015	0.001AlaXaa: 0.001 ± 0.0
Cys	1.214CysAla: 1.214 ± 0.015	0.482CysCys: 0.482 ± 0.011	1.097CysAsp: 1.097 ± 0.019	1.101CysGlu: 1.101 ± 0.015	0.709CysPhe: 0.709 ± 0.009	1.344CysGly: 1.344 ± 0.018	0.452CysHis: 0.452 ± 0.007	0.94CysIle: 0.94 ± 0.012	0.851CysLys: 0.851 ± 0.011	1.643CysLeu: 1.643 ± 0.017	0.378CysMet: 0.378 ± 0.007	0.808CysAsn: 0.808 ± 0.013	1.013CysPro: 1.013 ± 0.019	0.665CysGln: 0.665 ± 0.011	1.079CysArg: 1.079 ± 0.017	1.463CysSer: 1.463 ± 0.018	1.062CysThr: 1.062 ± 0.013	1.209CysVal: 1.209 ± 0.016	0.224CysTrp: 0.224 ± 0.005	0.582CysTyr: 0.582 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.676AspAla: 3.676 ± 0.024	1.01AspCys: 1.01 ± 0.014	4.035AspAsp: 4.035 ± 0.043	4.386AspGlu: 4.386 ± 0.034	1.906AspPhe: 1.906 ± 0.018	3.962AspGly: 3.962 ± 0.027	1.211AspHis: 1.211 ± 0.012	2.672AspIle: 2.672 ± 0.019	2.424AspLys: 2.424 ± 0.026	4.45AspLeu: 4.45 ± 0.03	1.121AspMet: 1.121 ± 0.012	2.025AspAsn: 2.025 ± 0.019	2.573AspPro: 2.573 ± 0.024	1.874AspGln: 1.874 ± 0.018	3.145AspArg: 3.145 ± 0.029	3.919AspSer: 3.919 ± 0.026	2.757AspThr: 2.757 ± 0.023	3.366AspVal: 3.366 ± 0.021	0.598AspTrp: 0.598 ± 0.009	1.707AspTyr: 1.707 ± 0.014	0.001AspXaa: 0.001 ± 0.0
Glu	4.356GluAla: 4.356 ± 0.044	1.178GluCys: 1.178 ± 0.018	3.725GluAsp: 3.725 ± 0.029	5.972GluGlu: 5.972 ± 0.076	1.978GluPhe: 1.978 ± 0.017	3.132GluGly: 3.132 ± 0.023	1.564GluHis: 1.564 ± 0.016	3.039GluIle: 3.039 ± 0.024	3.961GluLys: 3.961 ± 0.048	6.128GluLeu: 6.128 ± 0.05	1.575GluMet: 1.575 ± 0.014	2.684GluAsn: 2.684 ± 0.022	2.978GluPro: 2.978 ± 0.035	3.586GluGln: 3.586 ± 0.043	4.224GluArg: 4.224 ± 0.036	4.211GluSer: 4.211 ± 0.034	3.389GluThr: 3.389 ± 0.034	3.515GluVal: 3.515 ± 0.035	0.6GluTrp: 0.6 ± 0.01	1.854GluTyr: 1.854 ± 0.015	0.0GluXaa: 0.0 ± 0.0
Phe	2.365PheAla: 2.365 ± 0.021	0.716PheCys: 0.716 ± 0.01	2.024PheAsp: 2.024 ± 0.015	2.069PheGlu: 2.069 ± 0.018	1.426PhePhe: 1.426 ± 0.017	2.436PheGly: 2.436 ± 0.022	0.91PheHis: 0.91 ± 0.011	1.803PheIle: 1.803 ± 0.018	1.613PheLys: 1.613 ± 0.013	3.309PheLeu: 3.309 ± 0.026	0.775PheMet: 0.775 ± 0.01	1.581PheAsn: 1.581 ± 0.015	1.556PhePro: 1.556 ± 0.014	1.367PheGln: 1.367 ± 0.012	2.01PheArg: 2.01 ± 0.016	2.506PheSer: 2.506 ± 0.018	1.907PheThr: 1.907 ± 0.017	2.451PheVal: 2.451 ± 0.017	0.407PheTrp: 0.407 ± 0.007	1.224PheTyr: 1.224 ± 0.014	0.001PheXaa: 0.001 ± 0.0
Gly	5.263GlyAla: 5.263 ± 0.039	1.073GlyCys: 1.073 ± 0.016	3.154GlyAsp: 3.154 ± 0.026	3.541GlyGlu: 3.541 ± 0.029	2.139GlyPhe: 2.139 ± 0.02	8.704GlyGly: 8.704 ± 0.095	1.569GlyHis: 1.569 ± 0.018	2.792GlyIle: 2.792 ± 0.021	3.361GlyLys: 3.361 ± 0.021	4.824GlyLeu: 4.824 ± 0.037	1.519GlyMet: 1.519 ± 0.016	2.836GlyAsn: 2.836 ± 0.024	3.106GlyPro: 3.106 ± 0.036	2.477GlyGln: 2.477 ± 0.02	3.451GlyArg: 3.451 ± 0.028	6.505GlySer: 6.505 ± 0.05	4.326GlyThr: 4.326 ± 0.036	3.819GlyVal: 3.819 ± 0.024	0.688GlyTrp: 0.688 ± 0.01	2.024GlyTyr: 2.024 ± 0.019	0.001GlyXaa: 0.001 ± 0.0
His	1.571HisAla: 1.571 ± 0.013	0.568HisCys: 0.568 ± 0.009	1.172HisAsp: 1.172 ± 0.011	1.359HisGlu: 1.359 ± 0.014	0.934HisPhe: 0.934 ± 0.01	1.699HisGly: 1.699 ± 0.018	2.2HisHis: 2.2 ± 0.042	1.205HisIle: 1.205 ± 0.011	1.096HisLys: 1.096 ± 0.011	2.429HisLeu: 2.429 ± 0.018	0.585HisMet: 0.585 ± 0.009	1.096HisAsn: 1.096 ± 0.012	1.659HisPro: 1.659 ± 0.018	1.773HisGln: 1.773 ± 0.024	1.687HisArg: 1.687 ± 0.014	2.017HisSer: 2.017 ± 0.02	1.367HisThr: 1.367 ± 0.012	1.408HisVal: 1.408 ± 0.012	0.274HisTrp: 0.274 ± 0.006	0.928HisTyr: 0.928 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	3.666IleAla: 3.666 ± 0.022	1.029IleCys: 1.029 ± 0.014	2.904IleAsp: 2.904 ± 0.022	3.22IleGlu: 3.22 ± 0.032	1.808IlePhe: 1.808 ± 0.018	2.968IleGly: 2.968 ± 0.021	1.137IleHis: 1.137 ± 0.013	2.503IleIle: 2.503 ± 0.021	2.501IleLys: 2.501 ± 0.018	4.141IleLeu: 4.141 ± 0.027	1.046IleMet: 1.046 ± 0.012	2.215IleAsn: 2.215 ± 0.017	2.535IlePro: 2.535 ± 0.02	1.89IleGln: 1.89 ± 0.017	2.651IleArg: 2.651 ± 0.02	3.563IleSer: 3.563 ± 0.024	2.809IleThr: 2.809 ± 0.022	3.666IleVal: 3.666 ± 0.023	0.436IleTrp: 0.436 ± 0.008	1.456IleTyr: 1.456 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.505LysAla: 3.505 ± 0.026	0.971LysCys: 0.971 ± 0.014	2.687LysAsp: 2.687 ± 0.021	3.677LysGlu: 3.677 ± 0.034	1.703LysPhe: 1.703 ± 0.016	2.512LysGly: 2.512 ± 0.027	1.439LysHis: 1.439 ± 0.013	2.659LysIle: 2.659 ± 0.021	3.801LysLys: 3.801 ± 0.053	5.141LysLeu: 5.141 ± 0.034	1.323LysMet: 1.323 ± 0.012	2.141LysAsn: 2.141 ± 0.015	2.93LysPro: 2.93 ± 0.036	2.761LysGln: 2.761 ± 0.023	3.515LysArg: 3.515 ± 0.023	3.692LysSer: 3.692 ± 0.032	2.873LysThr: 2.873 ± 0.023	3.068LysVal: 3.068 ± 0.039	0.495LysTrp: 0.495 ± 0.008	1.67LysTyr: 1.67 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	6.685LeuAla: 6.685 ± 0.036	1.656LeuCys: 1.656 ± 0.017	4.734LeuAsp: 4.734 ± 0.034	5.912LeuGlu: 5.912 ± 0.051	3.201LeuPhe: 3.201 ± 0.026	4.708LeuGly: 4.708 ± 0.028	2.483LeuHis: 2.483 ± 0.021	4.054LeuIle: 4.054 ± 0.027	5.095LeuLys: 5.095 ± 0.032	9.208LeuLeu: 9.208 ± 0.051	1.991LeuMet: 1.991 ± 0.017	3.924LeuAsn: 3.924 ± 0.025	4.915LeuPro: 4.915 ± 0.029	4.711LeuGln: 4.711 ± 0.034	5.243LeuArg: 5.243 ± 0.033	6.593LeuSer: 6.593 ± 0.038	4.914LeuThr: 4.914 ± 0.027	5.439LeuVal: 5.439 ± 0.034	0.873LeuTrp: 0.873 ± 0.011	2.643LeuTyr: 2.643 ± 0.021	0.001LeuXaa: 0.001 ± 0.0
Met	1.83MetAla: 1.83 ± 0.014	0.389MetCys: 0.389 ± 0.006	1.278MetAsp: 1.278 ± 0.013	1.504MetGlu: 1.504 ± 0.014	0.853MetPhe: 0.853 ± 0.01	1.285MetGly: 1.285 ± 0.014	0.587MetHis: 0.587 ± 0.008	1.072MetIle: 1.072 ± 0.013	1.348MetLys: 1.348 ± 0.012	2.178MetLeu: 2.178 ± 0.015	0.722MetMet: 0.722 ± 0.009	0.996MetAsn: 0.996 ± 0.012	1.123MetPro: 1.123 ± 0.013	1.064MetGln: 1.064 ± 0.012	1.24MetArg: 1.24 ± 0.01	1.625MetSer: 1.625 ± 0.013	1.13MetThr: 1.13 ± 0.012	1.464MetVal: 1.464 ± 0.013	0.206MetTrp: 0.206 ± 0.005	0.702MetTyr: 0.702 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.151AsnAla: 3.151 ± 0.024	0.806AsnCys: 0.806 ± 0.012	2.33AsnAsp: 2.33 ± 0.02	2.645AsnGlu: 2.645 ± 0.019	1.53AsnPhe: 1.53 ± 0.014	3.781AsnGly: 3.781 ± 0.036	1.143AsnHis: 1.143 ± 0.013	2.274AsnIle: 2.274 ± 0.017	1.954AsnLys: 1.954 ± 0.017	3.814AsnLeu: 3.814 ± 0.026	0.974AsnMet: 0.974 ± 0.012	2.784AsnAsn: 2.784 ± 0.036	2.132AsnPro: 2.132 ± 0.017	1.749AsnGln: 1.749 ± 0.016	2.546AsnArg: 2.546 ± 0.018	3.463AsnSer: 3.463 ± 0.03	2.265AsnThr: 2.265 ± 0.018	2.989AsnVal: 2.989 ± 0.02	0.424AsnTrp: 0.424 ± 0.007	1.395AsnTyr: 1.395 ± 0.015	0.001AsnXaa: 0.001 ± 0.0
Pro	4.89ProAla: 4.89 ± 0.033	0.726ProCys: 0.726 ± 0.017	2.694ProAsp: 2.694 ± 0.02	3.323ProGlu: 3.323 ± 0.039	1.717ProPhe: 1.717 ± 0.014	3.433ProGly: 3.433 ± 0.044	1.318ProHis: 1.318 ± 0.015	2.493ProIle: 2.493 ± 0.019	2.75ProLys: 2.75 ± 0.03	4.295ProLeu: 4.295 ± 0.029	1.132ProMet: 1.132 ± 0.013	2.288ProAsn: 2.288 ± 0.02	4.953ProPro: 4.953 ± 0.067	2.328ProGln: 2.328 ± 0.026	2.331ProArg: 2.331 ± 0.022	4.783ProSer: 4.783 ± 0.036	3.656ProThr: 3.656 ± 0.029	3.678ProVal: 3.678 ± 0.029	0.449ProTrp: 0.449 ± 0.008	1.559ProTyr: 1.559 ± 0.016	0.0ProXaa: 0.0 ± 0.0
Gln	2.876GlnAla: 2.876 ± 0.019	0.837GlnCys: 0.837 ± 0.013	1.847GlnAsp: 1.847 ± 0.017	2.817GlnGlu: 2.817 ± 0.033	1.476GlnPhe: 1.476 ± 0.014	1.937GlnGly: 1.937 ± 0.017	1.954GlnHis: 1.954 ± 0.026	2.082GlnIle: 2.082 ± 0.017	2.303GlnLys: 2.303 ± 0.019	4.687GlnLeu: 4.687 ± 0.033	1.073GlnMet: 1.073 ± 0.012	1.809GlnAsn: 1.809 ± 0.017	2.88GlnPro: 2.88 ± 0.027	7.625GlnGln: 7.625 ± 0.177	2.859GlnArg: 2.859 ± 0.021	3.278GlnSer: 3.278 ± 0.022	2.514GlnThr: 2.514 ± 0.019	2.135GlnVal: 2.135 ± 0.019	0.434GlnTrp: 0.434 ± 0.008	1.455GlnTyr: 1.455 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	3.607ArgAla: 3.607 ± 0.026	1.162ArgCys: 1.162 ± 0.019	2.888ArgAsp: 2.888 ± 0.025	3.562ArgGlu: 3.562 ± 0.029	2.129ArgPhe: 2.129 ± 0.018	2.852ArgGly: 2.852 ± 0.029	1.564ArgHis: 1.564 ± 0.014	2.762ArgIle: 2.762 ± 0.022	3.738ArgLys: 3.738 ± 0.024	5.085ArgLeu: 5.085 ± 0.032	1.349ArgMet: 1.349 ± 0.012	2.66ArgAsn: 2.66 ± 0.02	2.818ArgPro: 2.818 ± 0.037	2.611ArgGln: 2.611 ± 0.019	4.077ArgArg: 4.077 ± 0.03	4.397ArgSer: 4.397 ± 0.03	3.344ArgThr: 3.344 ± 0.018	2.959ArgVal: 2.959 ± 0.021	0.673ArgTrp: 0.673 ± 0.009	1.95ArgTyr: 1.95 ± 0.018	0.001ArgXaa: 0.001 ± 0.0
Ser	6.122SerAla: 6.122 ± 0.039	1.335SerCys: 1.335 ± 0.017	4.008SerAsp: 4.008 ± 0.027	4.24SerGlu: 4.24 ± 0.035	2.806SerPhe: 2.806 ± 0.021	5.932SerGly: 5.932 ± 0.045	1.767SerHis: 1.767 ± 0.015	3.815SerIle: 3.815 ± 0.024	3.865SerLys: 3.865 ± 0.026	6.546SerLeu: 6.546 ± 0.034	1.7SerMet: 1.7 ± 0.014	3.947SerAsn: 3.947 ± 0.029	4.492SerPro: 4.492 ± 0.041	2.855SerGln: 2.855 ± 0.023	3.872SerArg: 3.872 ± 0.031	10.49SerSer: 10.49 ± 0.108	5.519SerThr: 5.519 ± 0.043	4.957SerVal: 4.957 ± 0.024	0.71SerTrp: 0.71 ± 0.009	2.24SerTyr: 2.24 ± 0.023	0.001SerXaa: 0.001 ± 0.0
Thr	5.12ThrAla: 5.12 ± 0.036	0.977ThrCys: 0.977 ± 0.015	2.921ThrAsp: 2.921 ± 0.02	3.386ThrGlu: 3.386 ± 0.039	2.037ThrPhe: 2.037 ± 0.018	4.134ThrGly: 4.134 ± 0.031	1.29ThrHis: 1.29 ± 0.012	3.382ThrIle: 3.382 ± 0.022	2.79ThrLys: 2.79 ± 0.021	5.195ThrLeu: 5.195 ± 0.028	1.344ThrMet: 1.344 ± 0.012	2.659ThrAsn: 2.659 ± 0.022	3.604ThrPro: 3.604 ± 0.033	2.01ThrGln: 2.01 ± 0.017	2.532ThrArg: 2.532 ± 0.023	4.885ThrSer: 4.885 ± 0.034	5.401ThrThr: 5.401 ± 0.091	4.675ThrVal: 4.675 ± 0.033	0.455ThrTrp: 0.455 ± 0.008	1.52ThrTyr: 1.52 ± 0.014	0.001ThrXaa: 0.001 ± 0.0
Val	5.028ValAla: 5.028 ± 0.029	1.345ValCys: 1.345 ± 0.016	3.28ValAsp: 3.28 ± 0.019	4.03ValGlu: 4.03 ± 0.062	2.182ValPhe: 2.182 ± 0.018	3.823ValGly: 3.823 ± 0.025	1.594ValHis: 1.594 ± 0.014	2.932ValIle: 2.932 ± 0.023	3.195ValLys: 3.195 ± 0.026	5.779ValLeu: 5.779 ± 0.034	1.351ValMet: 1.351 ± 0.015	2.514ValAsn: 2.514 ± 0.018	3.753ValPro: 3.753 ± 0.029	2.806ValGln: 2.806 ± 0.024	3.53ValArg: 3.53 ± 0.022	4.616ValSer: 4.616 ± 0.023	3.757ValThr: 3.757 ± 0.024	4.397ValVal: 4.397 ± 0.033	0.641ValTrp: 0.641 ± 0.009	1.874ValTyr: 1.874 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	0.531TrpAla: 0.531 ± 0.008	0.203TrpCys: 0.203 ± 0.004	0.52TrpAsp: 0.52 ± 0.008	0.559TrpGlu: 0.559 ± 0.008	0.404TrpPhe: 0.404 ± 0.008	0.5TrpGly: 0.5 ± 0.009	0.248TrpHis: 0.248 ± 0.006	0.604TrpIle: 0.604 ± 0.009	0.58TrpLys: 0.58 ± 0.01	0.992TrpLeu: 0.992 ± 0.012	0.285TrpMet: 0.285 ± 0.006	0.539TrpAsn: 0.539 ± 0.008	0.378TrpPro: 0.378 ± 0.008	0.403TrpGln: 0.403 ± 0.007	0.607TrpArg: 0.607 ± 0.009	0.711TrpSer: 0.711 ± 0.01	0.575TrpThr: 0.575 ± 0.009	0.521TrpVal: 0.521 ± 0.008	0.162TrpTrp: 0.162 ± 0.004	0.358TrpTyr: 0.358 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.942TyrAla: 1.942 ± 0.015	0.676TyrCys: 0.676 ± 0.009	1.868TyrAsp: 1.868 ± 0.016	1.913TyrGlu: 1.913 ± 0.014	1.23TyrPhe: 1.23 ± 0.013	2.195TyrGly: 2.195 ± 0.02	0.925TyrHis: 0.925 ± 0.012	1.443TyrIle: 1.443 ± 0.014	1.383TyrLys: 1.383 ± 0.014	2.701TyrLeu: 2.701 ± 0.022	0.645TyrMet: 0.645 ± 0.008	1.396TyrAsn: 1.396 ± 0.014	1.372TyrPro: 1.372 ± 0.015	1.312TyrGln: 1.312 ± 0.013	1.983TyrArg: 1.983 ± 0.017	2.131TyrSer: 2.131 ± 0.018	1.653TyrThr: 1.653 ± 0.016	1.853TyrVal: 1.853 ± 0.018	0.353TyrTrp: 0.353 ± 0.008	1.215TyrTyr: 1.215 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.027XaaXaa: 0.027 ± 0.007

Statistics based on 17099 proteins (9740585 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---