Prevotella sp. CAG:485

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7F3R1|R7F3R1_9BACT D-Lysine 56-aminomutase alpha subunit OS=Prevotella sp. CAG:485 OX=1262927 GN=BN677_00049 PE=4 SV=1
MM1 pKa = 7.65VLDD4 pKa = 4.33EE5 pKa = 4.87RR6 pKa = 11.84DD7 pKa = 3.84SVNLNVVDD15 pKa = 6.0LGAEE19 pKa = 4.25LDD21 pKa = 3.95PFVLFATHH29 pKa = 6.4YY30 pKa = 8.35GAEE33 pKa = 4.07IRR35 pKa = 11.84SVDD38 pKa = 3.43ADD40 pKa = 3.6DD41 pKa = 4.21TVLHH45 pKa = 6.17FLFIEE50 pKa = 4.39EE51 pKa = 4.11VGLLLMYY58 pKa = 9.57QTDD61 pKa = 3.79CRR63 pKa = 11.84DD64 pKa = 3.45TFVLLSCEE72 pKa = 3.92NNQRR76 pKa = 11.84GILTVQPVPLSDD88 pKa = 5.84DD89 pKa = 3.65FAQQIEE95 pKa = 4.46QPPLHH100 pKa = 6.64PACGG104 pKa = 3.38

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7F4E8|R7F4E8_9BACT M6 family metalloprotease domain-containing protein OS=Prevotella sp. CAG:485 OX=1262927 GN=BN677_00325 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.36KK6 pKa = 9.96RR7 pKa = 11.84KK8 pKa = 6.44GHH10 pKa = 6.2KK11 pKa = 9.14MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1956

0

1956

695163

29

2520

355.4

39.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.817 ± 0.058

1.274 ± 0.021

5.569 ± 0.048

5.78 ± 0.066

4.101 ± 0.036

7.358 ± 0.066

1.903 ± 0.025

6.079 ± 0.037

5.379 ± 0.05

9.21 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.029

4.768 ± 0.048

4.371 ± 0.033

3.4 ± 0.032

5.454 ± 0.057

6.065 ± 0.049

5.835 ± 0.058

6.717 ± 0.046

1.196 ± 0.021

3.895 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski