Torque teno virus 28
Average proteome isoelectric point is 7.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8V7I1|Q8V7I1_9VIRU Capsid protein OS=Torque teno virus 28 OX=687367 PE=3 SV=1
MM1 pKa = 7.33 SWRR4 pKa = 11.84 PPAYY8 pKa = 10.19 NLAQRR13 pKa = 11.84 EE14 pKa = 4.27 EE15 pKa = 4.03 HH16 pKa = 6.36 WYY18 pKa = 10.05 KK19 pKa = 11.02 AILEE23 pKa = 4.29 SHH25 pKa = 5.85 STFCGCSDD33 pKa = 3.83 VVRR36 pKa = 11.84 HH37 pKa = 5.51 FCVLASRR44 pKa = 11.84 LATNPSVIPALPAPPEE60 pKa = 3.94 QQPRR64 pKa = 11.84 PGGDD68 pKa = 3.09 TEE70 pKa = 4.61 GAPGDD75 pKa = 4.46 PGDD78 pKa = 4.48 AGAGRR83 pKa = 11.84 YY84 pKa = 9.19 AEE86 pKa = 4.5 EE87 pKa = 4.4 DD88 pKa = 3.66 LEE90 pKa = 4.37 EE91 pKa = 4.54 LFAAAAKK98 pKa = 10.76 DD99 pKa = 3.78 DD100 pKa = 3.79 MM101 pKa = 6.43
Molecular weight: 10.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.556
IPC2_protein 4.66
IPC_protein 4.52
Toseland 4.393
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.622
Wikipedia 4.355
Rodwell 4.38
Grimsley 4.304
Solomon 4.469
Lehninger 4.431
Nozaki 4.596
DTASelect 4.736
Thurlkill 4.393
EMBOSS 4.38
Sillero 4.647
Patrickios 3.528
IPC_peptide 4.482
IPC2_peptide 4.635
IPC2.peptide.svr19 4.593
Protein with the highest isoelectric point:
>tr|Q8V7I3|Q8V7I3_9VIRU Uncharacterized protein OS=Torque teno virus 28 OX=687367 PE=4 SV=1
MM1 pKa = 7.33 SWRR4 pKa = 11.84 PPAYY8 pKa = 10.19 NLAQRR13 pKa = 11.84 EE14 pKa = 4.27 EE15 pKa = 4.03 HH16 pKa = 6.36 WYY18 pKa = 10.05 KK19 pKa = 11.02 AILEE23 pKa = 4.29 SHH25 pKa = 5.85 STFCGCSDD33 pKa = 3.83 VVRR36 pKa = 11.84 HH37 pKa = 5.51 FCVLASRR44 pKa = 11.84 LATNPSVIPALPAPPEE60 pKa = 3.94 QQPRR64 pKa = 11.84 PGGDD68 pKa = 3.09 TEE70 pKa = 4.61 GAPGDD75 pKa = 4.46 PGDD78 pKa = 4.48 AGAGRR83 pKa = 11.84 YY84 pKa = 9.19 AEE86 pKa = 4.5 EE87 pKa = 4.4 DD88 pKa = 3.66 LEE90 pKa = 4.37 EE91 pKa = 4.54 LFAAAAKK98 pKa = 10.66 DD99 pKa = 3.58 DD100 pKa = 3.62 MRR102 pKa = 11.84 RR103 pKa = 11.84 VQKK106 pKa = 9.49 TPNGNPVRR114 pKa = 11.84 GSHH117 pKa = 6.1 RR118 pKa = 11.84 KK119 pKa = 7.58 PRR121 pKa = 11.84 RR122 pKa = 11.84 RR123 pKa = 11.84 RR124 pKa = 11.84 LLFTQSTPKK133 pKa = 10.18 RR134 pKa = 11.84 ARR136 pKa = 11.84 EE137 pKa = 3.75 QPVRR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 DD144 pKa = 3.2 SPRR147 pKa = 11.84 RR148 pKa = 11.84 GDD150 pKa = 3.53 PKK152 pKa = 10.57 TATTQAAQPAATAAASPQRR171 pKa = 11.84 GTQTSPGRR179 pKa = 11.84 RR180 pKa = 11.84 PPTPEE185 pKa = 4.17 RR186 pKa = 11.84 SPLGPPPIIIQGDD199 pKa = 4.68 PIPDD203 pKa = 3.78 LLFPSTGKK211 pKa = 9.8 KK212 pKa = 9.82 RR213 pKa = 11.84 KK214 pKa = 8.94 FSKK217 pKa = 10.58 FDD219 pKa = 3.19 WEE221 pKa = 4.34 TEE223 pKa = 3.97 AEE225 pKa = 3.77 IGRR228 pKa = 11.84 WLRR231 pKa = 11.84 RR232 pKa = 11.84 PMRR235 pKa = 11.84 FYY237 pKa = 10.66 PSDD240 pKa = 3.84 PPHH243 pKa = 6.43 YY244 pKa = 9.4 PWLPPKK250 pKa = 10.23 RR251 pKa = 11.84 DD252 pKa = 3.07 IPKK255 pKa = 8.76 ICKK258 pKa = 9.96 VNFKK262 pKa = 10.86 INFTEE267 pKa = 3.93
Molecular weight: 29.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.326
IPC_protein 9.589
Toseland 10.335
ProMoST 9.955
Dawson 10.467
Bjellqvist 10.131
Wikipedia 10.613
Rodwell 10.76
Grimsley 10.511
Solomon 10.526
Lehninger 10.496
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.379
Patrickios 10.452
IPC_peptide 10.526
IPC2_peptide 8.96
IPC2.peptide.svr19 8.415
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1379
101
734
344.8
39.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.454 ± 1.981
1.668 ± 0.249
4.569 ± 0.446
5.511 ± 0.543
3.191 ± 0.536
5.656 ± 0.523
2.103 ± 0.116
3.481 ± 0.491
6.454 ± 0.758
7.034 ± 1.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.74 ± 0.426
4.206 ± 0.749
9.065 ± 1.942
4.714 ± 0.312
8.92 ± 1.147
7.977 ± 2.114
6.454 ± 0.479
3.626 ± 0.448
2.538 ± 0.409
4.641 ± 1.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here