Bacillus aquimaris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Rossellomorea

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J6VQX4|A0A1J6VQX4_9BACI Uncharacterized protein OS=Bacillus aquimaris OX=189382 GN=BHE18_12935 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.49NFLLIVFGLICVGLLYY18 pKa = 10.37IGNQYY23 pKa = 7.44WQEE26 pKa = 4.25RR27 pKa = 11.84IAGAPAAQAGGEE39 pKa = 4.32VKK41 pKa = 10.54EE42 pKa = 4.46EE43 pKa = 4.07NAPEE47 pKa = 3.85PAEE50 pKa = 4.03AVKK53 pKa = 10.61EE54 pKa = 4.02EE55 pKa = 4.28PLISNWPAKK64 pKa = 10.2AQDD67 pKa = 3.48RR68 pKa = 11.84FKK70 pKa = 11.11EE71 pKa = 4.08AVNAGNAYY79 pKa = 10.23RR80 pKa = 11.84IALVGSDD87 pKa = 4.04ALGSGNGGWSDD98 pKa = 3.52QLKK101 pKa = 10.2EE102 pKa = 4.23EE103 pKa = 4.45LSSGYY108 pKa = 10.35GDD110 pKa = 3.28QVEE113 pKa = 4.21VSIFEE118 pKa = 4.21EE119 pKa = 4.45DD120 pKa = 3.37TTSVEE125 pKa = 4.1FLEE128 pKa = 4.82GDD130 pKa = 3.6VYY132 pKa = 11.5EE133 pKa = 5.32DD134 pKa = 3.29VLAFQPDD141 pKa = 3.7LVLFEE146 pKa = 5.29PFTLTDD152 pKa = 3.27NSIGVPVEE160 pKa = 4.19RR161 pKa = 11.84NHH163 pKa = 6.56EE164 pKa = 4.64SILTFYY170 pKa = 11.35EE171 pKa = 3.96EE172 pKa = 4.11LQEE175 pKa = 4.29EE176 pKa = 4.38NSSAEE181 pKa = 4.73LILQPSYY188 pKa = 10.27PIEE191 pKa = 4.15GATYY195 pKa = 10.08YY196 pKa = 9.97PKK198 pKa = 10.47QVDD201 pKa = 3.77GLKK204 pKa = 9.65TFAGEE209 pKa = 4.24NDD211 pKa = 3.71FTYY214 pKa = 10.81LDD216 pKa = 4.53HH217 pKa = 6.02WAEE220 pKa = 3.88WPEE223 pKa = 3.84EE224 pKa = 4.2EE225 pKa = 5.19EE226 pKa = 4.69LGDD229 pKa = 3.83YY230 pKa = 10.4LLPSQEE236 pKa = 4.25GPNEE240 pKa = 3.96QGHH243 pKa = 5.85EE244 pKa = 3.88LWADD248 pKa = 3.29YY249 pKa = 11.63LMDD252 pKa = 3.87YY253 pKa = 10.26FIAEE257 pKa = 4.06

Molecular weight: 28.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J6VQP5|A0A1J6VQP5_9BACI Uncharacterized protein OS=Bacillus aquimaris OX=189382 GN=BHE18_12590 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84FFEE5 pKa = 4.45CRR7 pKa = 11.84LLISVQDD14 pKa = 3.47YY15 pKa = 10.65AFRR18 pKa = 11.84GRR20 pKa = 11.84PVSTIFLIVPYY31 pKa = 10.06IAAIDD36 pKa = 3.65FRR38 pKa = 11.84GRR40 pKa = 11.84LRR42 pKa = 11.84FPRR45 pKa = 11.84VTRR48 pKa = 11.84EE49 pKa = 3.58PPAFFIGGGTVQLQGLEE66 pKa = 3.95AHH68 pKa = 6.86VISQTGQEE76 pKa = 4.19GKK78 pKa = 10.21EE79 pKa = 3.8RR80 pKa = 11.84LRR82 pKa = 11.84DD83 pKa = 3.66RR84 pKa = 11.84FALCHH89 pKa = 6.2TPLSKK94 pKa = 10.8APPLFF99 pKa = 4.18

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4196

0

4196

1192751

26

2530

284.3

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.952 ± 0.04

0.702 ± 0.012

5.307 ± 0.036

7.856 ± 0.046

4.567 ± 0.028

7.312 ± 0.038

2.148 ± 0.02

7.431 ± 0.036

6.936 ± 0.035

9.746 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.018

4.015 ± 0.026

3.668 ± 0.022

3.349 ± 0.025

4.155 ± 0.028

6.238 ± 0.034

5.171 ± 0.026

7.015 ± 0.032

1.06 ± 0.015

3.486 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski