Nitrosomonas cryotolerans ATCC 49181
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2414 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6G367|A0A1N6G367_9PROT Transposase IS605 OrfB family central region OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=SAMN02743940_0528 PE=3 SV=1
MM1 pKa = 7.2 ISYY4 pKa = 10.33 RR5 pKa = 11.84 RR6 pKa = 11.84 FKK8 pKa = 10.82 SQISITLLVTALSWLVCNLAVAAPPTNDD36 pKa = 2.69 TFTNSTSVTVGFNEE50 pKa = 4.34 VLDD53 pKa = 4.08 TTEE56 pKa = 4.19 ATTDD60 pKa = 3.38 SDD62 pKa = 4.42 DD63 pKa = 3.72 AQLNMSCGAPASDD76 pKa = 3.03 ASVWYY81 pKa = 10.33 SFNATSDD88 pKa = 3.61 TNVVVDD94 pKa = 4.42 VSQSNYY100 pKa = 9.3 SAGVLVSEE108 pKa = 5.46 GSQDD112 pKa = 3.49 NLQTIACGPGAVPFFATAGTTYY134 pKa = 10.75 YY135 pKa = 11.11 VLAIDD140 pKa = 4.71 DD141 pKa = 3.96 QLDD144 pKa = 3.58 GGGNGGLLSISFNEE158 pKa = 4.24 VAPTTLDD165 pKa = 3.6 DD166 pKa = 3.95 FTVNPVGIVNTRR178 pKa = 11.84 TGVATISGTYY188 pKa = 7.79 TCNNGDD194 pKa = 3.9 FLGLFADD201 pKa = 4.8 ASQDD205 pKa = 3.25 VGRR208 pKa = 11.84 IATIRR213 pKa = 11.84 GDD215 pKa = 3.85 FFFFDD220 pKa = 4.1 SGTCNGTSHH229 pKa = 7.27 PWSGNVVPQNGKK241 pKa = 9.31 FAGGKK246 pKa = 9.99 AMTMTFAFTCGPFQCADD263 pKa = 3.49 GYY265 pKa = 10.59 IEE267 pKa = 5.14 QKK269 pKa = 9.68 IQLRR273 pKa = 11.84 GGKK276 pKa = 9.2 KK277 pKa = 9.65
Molecular weight: 29.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.783
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.986
Rodwell 3.834
Grimsley 3.681
Solomon 4.012
Lehninger 3.973
Nozaki 4.139
DTASelect 4.431
Thurlkill 3.846
EMBOSS 3.999
Sillero 4.139
Patrickios 1.138
IPC_peptide 4.012
IPC2_peptide 4.113
IPC2.peptide.svr19 4.006
Protein with the highest isoelectric point:
>tr|A0A1N6GXW4|A0A1N6GXW4_9PROT 3-phosphoshikimate 1-carboxyvinyltransferase OS=Nitrosomonas cryotolerans ATCC 49181 OX=1131553 GN=aroA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.6 QPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 THH16 pKa = 5.9 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.48 TRR25 pKa = 11.84 GGAAVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.98 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LGVV44 pKa = 3.2
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2414
0
2414
781301
19
2921
323.7
36.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.658 ± 0.05
1.045 ± 0.016
5.141 ± 0.035
5.787 ± 0.038
4.058 ± 0.034
6.791 ± 0.049
2.577 ± 0.025
7.231 ± 0.043
4.968 ± 0.054
10.716 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.557 ± 0.022
4.204 ± 0.04
4.176 ± 0.029
4.321 ± 0.038
5.527 ± 0.036
6.272 ± 0.035
5.392 ± 0.031
6.359 ± 0.037
1.281 ± 0.022
2.94 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here