Bhendi yellow vein Delhi virus [2004:New Delhi]
Average proteome isoelectric point is 7.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8YB85|B8YB85_9GEMI AC4 OS=Bhendi yellow vein Delhi virus [2004:New Delhi] OX=587620 GN=AC4 PE=3 SV=1
MM1 pKa = 7.68 APPSRR6 pKa = 11.84 FRR8 pKa = 11.84 INAKK12 pKa = 10.3 NYY14 pKa = 8.19 FLTYY18 pKa = 9.06 PKK20 pKa = 10.53 CSLTKK25 pKa = 10.69 EE26 pKa = 4.09 EE27 pKa = 5.55 ALSQLQNLQTPTNKK41 pKa = 9.87 KK42 pKa = 9.42 FIKK45 pKa = 9.65 ICRR48 pKa = 11.84 EE49 pKa = 3.52 LHH51 pKa = 6.26 EE52 pKa = 4.93 NGEE55 pKa = 4.28 PHH57 pKa = 6.66 LHH59 pKa = 6.03 VLIQFEE65 pKa = 4.78 GKK67 pKa = 9.95 YY68 pKa = 9.77 KK69 pKa = 10.46 CQNQRR74 pKa = 11.84 FFDD77 pKa = 3.97 LVSPSRR83 pKa = 11.84 SVHH86 pKa = 4.99 FHH88 pKa = 6.71 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.7 KK106 pKa = 11.33 DD107 pKa = 3.49 GDD109 pKa = 3.67 TLEE112 pKa = 4.14 WGEE115 pKa = 3.84 FQIDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 4.05 AYY134 pKa = 10.69 AAALNAGSKK143 pKa = 10.33 SEE145 pKa = 3.85 ALKK148 pKa = 10.64 VIRR151 pKa = 11.84 EE152 pKa = 4.37 LAPKK156 pKa = 10.23 DD157 pKa = 3.59 YY158 pKa = 11.19 VLQFHH163 pKa = 6.76 NLNANLDD170 pKa = 4.3 RR171 pKa = 11.84 IFTPPLEE178 pKa = 4.56 VYY180 pKa = 10.28 VSPFSSSSFDD190 pKa = 3.41 QVPEE194 pKa = 3.95 EE195 pKa = 4.04 LEE197 pKa = 3.96 EE198 pKa = 4.17 WVSEE202 pKa = 4.2 NVVDD206 pKa = 4.8 AAARR210 pKa = 11.84 PLRR213 pKa = 11.84 PLSLVLEE220 pKa = 4.7 GDD222 pKa = 3.46 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 4.79 DD262 pKa = 4.33 VDD264 pKa = 3.73 PHH266 pKa = 6.08 FLKK269 pKa = 10.67 HH270 pKa = 5.9 FKK272 pKa = 10.69 EE273 pKa = 4.43 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPNSCYY311 pKa = 10.3 KK312 pKa = 10.59 EE313 pKa = 3.94 FLDD316 pKa = 4.84 EE317 pKa = 4.5 EE318 pKa = 4.61 KK319 pKa = 11.18 NSALKK324 pKa = 10.53 NWALKK329 pKa = 10.11 NAIFITLAEE338 pKa = 4.23 PLYY341 pKa = 11.11 SGANQSTAQGSQEE354 pKa = 3.75 TDD356 pKa = 3.09 QEE358 pKa = 4.42 EE359 pKa = 4.53 EE360 pKa = 4.19 SGSS363 pKa = 3.6
Molecular weight: 40.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.609
IPC2_protein 5.588
IPC_protein 5.614
Toseland 5.957
ProMoST 5.906
Dawson 5.817
Bjellqvist 5.842
Wikipedia 5.804
Rodwell 5.792
Grimsley 6.097
Solomon 5.817
Lehninger 5.804
Nozaki 6.071
DTASelect 6.249
Thurlkill 6.224
EMBOSS 6.186
Sillero 6.16
Patrickios 4.113
IPC_peptide 5.83
IPC2_peptide 6.16
IPC2.peptide.svr19 6.135
Protein with the highest isoelectric point:
>tr|B8YB82|B8YB82_9GEMI Replication enhancer OS=Bhendi yellow vein Delhi virus [2004:New Delhi] OX=587620 GN=AC3 PE=3 SV=1
MM1 pKa = 6.62 GTSHH5 pKa = 7.4 IKK7 pKa = 9.23 HH8 pKa = 5.19 QRR10 pKa = 11.84 ILGMILVFSCFLLIVNNIIVNPDD33 pKa = 3.2 KK34 pKa = 11.33 LLDD37 pKa = 3.47 QSLFLTRR44 pKa = 11.84 ILTTRR49 pKa = 11.84 NGCMPFPQHH58 pKa = 6.5 LVTIPMHH65 pKa = 5.98 ILHH68 pKa = 6.66 GRR70 pKa = 11.84 RR71 pKa = 11.84 TGFVVKK77 pKa = 10.41 HH78 pKa = 5.68 IKK80 pKa = 10.52 YY81 pKa = 8.07 LTKK84 pKa = 10.04 ILGFIYY90 pKa = 10.28 RR91 pKa = 11.84 SSITNKK97 pKa = 9.54 EE98 pKa = 3.56 KK99 pKa = 9.65 HH100 pKa = 5.68 HH101 pKa = 7.13 RR102 pKa = 11.84 IRR104 pKa = 11.84 MVLGLDD110 pKa = 3.63 VLVHH114 pKa = 6.48 PNLTT118 pKa = 3.57
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.853
IPC_protein 10.628
Toseland 10.979
ProMoST 10.657
Dawson 11.038
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.33
Grimsley 11.067
Solomon 11.184
Lehninger 11.155
Nozaki 10.965
DTASelect 10.73
Thurlkill 10.965
EMBOSS 11.374
Sillero 10.979
Patrickios 11.096
IPC_peptide 11.199
IPC2_peptide 9.809
IPC2.peptide.svr19 8.495
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1234
100
363
176.3
20.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.943 ± 1.025
2.35 ± 0.38
4.457 ± 0.417
4.619 ± 0.862
4.052 ± 0.528
4.862 ± 0.432
3.728 ± 0.651
5.348 ± 0.671
5.754 ± 0.726
8.671 ± 0.996
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.431 ± 0.69
6.24 ± 0.446
5.835 ± 0.485
4.943 ± 0.799
6.402 ± 0.98
8.671 ± 1.479
5.997 ± 1.125
5.916 ± 1.112
1.297 ± 0.168
3.485 ± 0.531
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here