Haloarcula californiae icosahedral virus 1
Average proteome isoelectric point is 4.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C7A3R0|A0A1C7A3R0_9VIRU VP12 OS=Haloarcula californiae icosahedral virus 1 OX=1735722 GN=SS136_019 PE=4 SV=1
MM1 pKa = 7.51 AAVLDD6 pKa = 3.82 KK7 pKa = 10.77 RR8 pKa = 11.84 IEE10 pKa = 4.07 KK11 pKa = 10.76 SPTAGNKK18 pKa = 8.06 YY19 pKa = 9.93 RR20 pKa = 11.84 KK21 pKa = 9.44 RR22 pKa = 11.84 CLACSRR28 pKa = 11.84 WLPMCSAGDD37 pKa = 3.74 FQTAEE42 pKa = 4.17 HH43 pKa = 5.43 QHH45 pKa = 5.59 VLPADD50 pKa = 3.66 VDD52 pKa = 4.06 PDD54 pKa = 4.42 DD55 pKa = 5.15 EE56 pKa = 5.93 DD57 pKa = 3.64 PTVPAEE63 pKa = 4.16 EE64 pKa = 4.15 FTGTVDD70 pKa = 4.11 GLATAASSDD79 pKa = 3.93 DD80 pKa = 4.18 APTAMTDD87 pKa = 2.98 GGEE90 pKa = 4.15 DD91 pKa = 4.7 LGDD94 pKa = 3.7 QDD96 pKa = 6.16 DD97 pKa = 4.68 EE98 pKa = 4.45 EE99 pKa = 6.44 HH100 pKa = 7.68 DD101 pKa = 4.04 DD102 pKa = 4.46 VNEE105 pKa = 4.03 FDD107 pKa = 5.48 CPEE110 pKa = 4.92 CDD112 pKa = 3.16 THH114 pKa = 6.53 LTGYY118 pKa = 9.85 PDD120 pKa = 3.68 EE121 pKa = 5.22 CPEE124 pKa = 4.32 CGVPYY129 pKa = 10.23 RR130 pKa = 11.84 WNNDD134 pKa = 2.4 EE135 pKa = 4.63 DD136 pKa = 3.75
Molecular weight: 14.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.605
ProMoST 3.948
Dawson 3.821
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A1C7A3R1|A0A1C7A3R1_9VIRU VP7 OS=Haloarcula californiae icosahedral virus 1 OX=1735722 GN=SS136_020 PE=1 SV=1
MM1 pKa = 7.64 ASINVSRR8 pKa = 11.84 ATSTMTSASFLKK20 pKa = 10.52 SAVLVVVGSLLAQVVTSYY38 pKa = 11.0 LRR40 pKa = 11.84 SNVRR44 pKa = 11.84 DD45 pKa = 3.49 ISVKK49 pKa = 10.68 GGDD52 pKa = 3.87 AIYY55 pKa = 10.67 SAVAAMLVLIVLPKK69 pKa = 10.54 KK70 pKa = 10.25 YY71 pKa = 10.41 GKK73 pKa = 9.36 PLALGSTATSVRR85 pKa = 11.84 VMLRR89 pKa = 11.84 EE90 pKa = 3.88 FGVVV94 pKa = 2.88
Molecular weight: 9.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 9.955
IPC_protein 10.526
Toseland 10.643
ProMoST 10.277
Dawson 10.774
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.169
Grimsley 10.833
Solomon 10.833
Lehninger 10.804
Nozaki 10.599
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.95
IPC_peptide 10.833
IPC2_peptide 9.151
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
9584
37
1608
203.9
22.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.549 ± 0.75
0.803 ± 0.223
10.831 ± 0.565
8.932 ± 0.53
2.755 ± 0.227
9.505 ± 0.578
1.816 ± 0.191
2.556 ± 0.216
2.588 ± 0.28
6.813 ± 0.334
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.68 ± 0.179
2.734 ± 0.346
4.821 ± 0.303
3.506 ± 0.241
6.469 ± 0.374
5.05 ± 0.31
6.375 ± 0.324
7.69 ± 0.301
1.513 ± 0.133
3.015 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here