Amino acid dipepetide frequency for Bacillaceae bacterium SAS-127

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.155AlaAla: 6.155 ± 0.105
0.665AlaCys: 0.665 ± 0.026
3.61AlaAsp: 3.61 ± 0.056
5.072AlaGlu: 5.072 ± 0.072
3.512AlaPhe: 3.512 ± 0.063
5.242AlaGly: 5.242 ± 0.079
1.407AlaHis: 1.407 ± 0.036
6.029AlaIle: 6.029 ± 0.07
5.015AlaLys: 5.015 ± 0.07
7.441AlaLeu: 7.441 ± 0.088
2.163AlaMet: 2.163 ± 0.048
2.859AlaAsn: 2.859 ± 0.053
2.11AlaPro: 2.11 ± 0.047
2.392AlaGln: 2.392 ± 0.053
2.727AlaArg: 2.727 ± 0.055
4.213AlaSer: 4.213 ± 0.067
3.805AlaThr: 3.805 ± 0.073
5.769AlaVal: 5.769 ± 0.081
0.595AlaTrp: 0.595 ± 0.024
2.264AlaTyr: 2.264 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.447CysAla: 0.447 ± 0.022
0.12CysCys: 0.12 ± 0.011
0.428CysAsp: 0.428 ± 0.019
0.495CysGlu: 0.495 ± 0.025
0.32CysPhe: 0.32 ± 0.018
0.701CysGly: 0.701 ± 0.036
0.209CysHis: 0.209 ± 0.014
0.473CysIle: 0.473 ± 0.024
0.315CysLys: 0.315 ± 0.017
0.759CysLeu: 0.759 ± 0.025
0.205CysMet: 0.205 ± 0.013
0.253CysAsn: 0.253 ± 0.015
0.371CysPro: 0.371 ± 0.019
0.329CysGln: 0.329 ± 0.019
0.3CysArg: 0.3 ± 0.017
0.546CysSer: 0.546 ± 0.026
0.423CysThr: 0.423 ± 0.019
0.484CysVal: 0.484 ± 0.023
0.078CysTrp: 0.078 ± 0.008
0.266CysTyr: 0.266 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
2.985AspAla: 2.985 ± 0.067
0.378AspCys: 0.378 ± 0.017
2.301AspAsp: 2.301 ± 0.056
4.281AspGlu: 4.281 ± 0.07
2.242AspPhe: 2.242 ± 0.047
3.19AspGly: 3.19 ± 0.055
1.261AspHis: 1.261 ± 0.035
3.907AspIle: 3.907 ± 0.074
2.652AspLys: 2.652 ± 0.058
4.696AspLeu: 4.696 ± 0.063
1.352AspMet: 1.352 ± 0.037
1.435AspAsn: 1.435 ± 0.043
1.908AspPro: 1.908 ± 0.044
2.443AspGln: 2.443 ± 0.05
2.344AspArg: 2.344 ± 0.052
2.356AspSer: 2.356 ± 0.053
2.153AspThr: 2.153 ± 0.045
3.816AspVal: 3.816 ± 0.068
0.635AspTrp: 0.635 ± 0.03
2.035AspTyr: 2.035 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
6.064GluAla: 6.064 ± 0.082
0.404GluCys: 0.404 ± 0.022
3.371GluAsp: 3.371 ± 0.06
7.664GluGlu: 7.664 ± 0.118
2.59GluPhe: 2.59 ± 0.054
4.419GluGly: 4.419 ± 0.075
1.665GluHis: 1.665 ± 0.039
5.259GluIle: 5.259 ± 0.075
6.944GluLys: 6.944 ± 0.094
7.294GluLeu: 7.294 ± 0.1
2.579GluMet: 2.579 ± 0.044
3.213GluAsn: 3.213 ± 0.06
2.008GluPro: 2.008 ± 0.048
4.351GluGln: 4.351 ± 0.073
3.771GluArg: 3.771 ± 0.064
3.314GluSer: 3.314 ± 0.06
3.89GluThr: 3.89 ± 0.066
5.509GluVal: 5.509 ± 0.087
0.9GluTrp: 0.9 ± 0.031
2.161GluTyr: 2.161 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.126PheAla: 3.126 ± 0.056
0.352PheCys: 0.352 ± 0.022
2.303PheAsp: 2.303 ± 0.041
2.858PheGlu: 2.858 ± 0.051
2.534PhePhe: 2.534 ± 0.069
3.2PheGly: 3.2 ± 0.061
1.088PheHis: 1.088 ± 0.03
3.957PheIle: 3.957 ± 0.082
2.238PheLys: 2.238 ± 0.045
4.734PheLeu: 4.734 ± 0.081
1.303PheMet: 1.303 ± 0.033
1.766PheAsn: 1.766 ± 0.038
1.675PhePro: 1.675 ± 0.041
1.769PheGln: 1.769 ± 0.042
1.521PheArg: 1.521 ± 0.041
3.365PheSer: 3.365 ± 0.065
2.606PheThr: 2.606 ± 0.049
3.311PheVal: 3.311 ± 0.06
0.471PheTrp: 0.471 ± 0.023
1.734PheTyr: 1.734 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.905GlyAla: 4.905 ± 0.081
0.632GlyCys: 0.632 ± 0.026
3.014GlyAsp: 3.014 ± 0.059
4.63GlyGlu: 4.63 ± 0.072
3.207GlyPhe: 3.207 ± 0.068
4.608GlyGly: 4.608 ± 0.089
1.401GlyHis: 1.401 ± 0.042
5.258GlyIle: 5.258 ± 0.081
4.812GlyLys: 4.812 ± 0.091
6.464GlyLeu: 6.464 ± 0.098
2.201GlyMet: 2.201 ± 0.047
2.283GlyAsn: 2.283 ± 0.05
1.638GlyPro: 1.638 ± 0.041
2.424GlyGln: 2.424 ± 0.05
2.679GlyArg: 2.679 ± 0.054
3.645GlySer: 3.645 ± 0.059
3.838GlyThr: 3.838 ± 0.062
5.096GlyVal: 5.096 ± 0.076
0.789GlyTrp: 0.789 ± 0.028
2.636GlyTyr: 2.636 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.309HisAla: 1.309 ± 0.046
0.241HisCys: 0.241 ± 0.015
0.99HisAsp: 0.99 ± 0.028
1.512HisGlu: 1.512 ± 0.039
1.139HisPhe: 1.139 ± 0.032
1.405HisGly: 1.405 ± 0.041
0.747HisHis: 0.747 ± 0.036
1.588HisIle: 1.588 ± 0.041
1.036HisLys: 1.036 ± 0.031
2.227HisLeu: 2.227 ± 0.049
0.525HisMet: 0.525 ± 0.019
0.69HisAsn: 0.69 ± 0.028
1.238HisPro: 1.238 ± 0.039
1.059HisGln: 1.059 ± 0.029
0.986HisArg: 0.986 ± 0.03
1.363HisSer: 1.363 ± 0.044
1.137HisThr: 1.137 ± 0.032
1.481HisVal: 1.481 ± 0.032
0.229HisTrp: 0.229 ± 0.014
0.88HisTyr: 0.88 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.973IleAla: 5.973 ± 0.081
0.645IleCys: 0.645 ± 0.024
4.3IleAsp: 4.3 ± 0.073
5.911IleGlu: 5.911 ± 0.073
3.303IlePhe: 3.303 ± 0.066
5.649IleGly: 5.649 ± 0.078
1.735IleHis: 1.735 ± 0.043
5.655IleIle: 5.655 ± 0.085
4.005IleLys: 4.005 ± 0.067
6.485IleLeu: 6.485 ± 0.097
1.76IleMet: 1.76 ± 0.046
2.937IleAsn: 2.937 ± 0.066
3.031IlePro: 3.031 ± 0.065
3.26IleGln: 3.26 ± 0.057
3.003IleArg: 3.003 ± 0.049
4.792IleSer: 4.792 ± 0.07
4.063IleThr: 4.063 ± 0.073
5.66IleVal: 5.66 ± 0.093
0.647IleTrp: 0.647 ± 0.027
2.332IleTyr: 2.332 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
4.79LysAla: 4.79 ± 0.07
0.323LysCys: 0.323 ± 0.018
3.505LysAsp: 3.505 ± 0.066
7.239LysGlu: 7.239 ± 0.087
1.864LysPhe: 1.864 ± 0.046
4.356LysGly: 4.356 ± 0.071
1.295LysHis: 1.295 ± 0.034
4.234LysIle: 4.234 ± 0.066
5.749LysLys: 5.749 ± 0.09
5.675LysLeu: 5.675 ± 0.08
2.38LysMet: 2.38 ± 0.046
3.021LysAsn: 3.021 ± 0.064
2.12LysPro: 2.12 ± 0.049
3.742LysGln: 3.742 ± 0.053
3.187LysArg: 3.187 ± 0.054
3.183LysSer: 3.183 ± 0.058
3.463LysThr: 3.463 ± 0.057
4.892LysVal: 4.892 ± 0.075
0.829LysTrp: 0.829 ± 0.032
2.098LysTyr: 2.098 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
7.836LeuAla: 7.836 ± 0.086
0.678LeuCys: 0.678 ± 0.026
4.596LeuAsp: 4.596 ± 0.064
6.542LeuGlu: 6.542 ± 0.096
5.147LeuPhe: 5.147 ± 0.094
6.019LeuGly: 6.019 ± 0.086
2.117LeuHis: 2.117 ± 0.043
6.898LeuIle: 6.898 ± 0.089
6.487LeuLys: 6.487 ± 0.086
10.922LeuLeu: 10.922 ± 0.154
2.722LeuMet: 2.722 ± 0.053
4.003LeuAsn: 4.003 ± 0.06
3.981LeuPro: 3.981 ± 0.058
4.343LeuGln: 4.343 ± 0.069
3.478LeuArg: 3.478 ± 0.069
6.956LeuSer: 6.956 ± 0.095
5.919LeuThr: 5.919 ± 0.09
6.345LeuVal: 6.345 ± 0.086
0.798LeuTrp: 0.798 ± 0.03
3.136LeuTyr: 3.136 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.21MetAla: 2.21 ± 0.051
0.18MetCys: 0.18 ± 0.013
1.437MetAsp: 1.437 ± 0.04
2.125MetGlu: 2.125 ± 0.05
1.133MetPhe: 1.133 ± 0.03
1.74MetGly: 1.74 ± 0.042
0.428MetHis: 0.428 ± 0.021
2.359MetIle: 2.359 ± 0.049
2.868MetLys: 2.868 ± 0.055
2.748MetLeu: 2.748 ± 0.055
1.069MetMet: 1.069 ± 0.03
1.715MetAsn: 1.715 ± 0.047
1.034MetPro: 1.034 ± 0.043
0.952MetGln: 0.952 ± 0.03
1.151MetArg: 1.151 ± 0.032
1.788MetSer: 1.788 ± 0.044
1.846MetThr: 1.846 ± 0.043
1.818MetVal: 1.818 ± 0.038
0.213MetTrp: 0.213 ± 0.013
0.832MetTyr: 0.832 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.469AsnAla: 2.469 ± 0.055
0.318AsnCys: 0.318 ± 0.02
2.093AsnAsp: 2.093 ± 0.052
3.54AsnGlu: 3.54 ± 0.062
1.471AsnPhe: 1.471 ± 0.039
3.024AsnGly: 3.024 ± 0.057
0.939AsnHis: 0.939 ± 0.029
3.231AsnIle: 3.231 ± 0.061
2.683AsnLys: 2.683 ± 0.046
3.296AsnLeu: 3.296 ± 0.061
1.224AsnMet: 1.224 ± 0.04
1.67AsnAsn: 1.67 ± 0.046
1.784AsnPro: 1.784 ± 0.046
1.777AsnGln: 1.777 ± 0.045
1.843AsnArg: 1.843 ± 0.044
2.034AsnSer: 2.034 ± 0.045
1.831AsnThr: 1.831 ± 0.046
2.781AsnVal: 2.781 ± 0.047
0.466AsnTrp: 0.466 ± 0.021
1.403AsnTyr: 1.403 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
2.375ProAla: 2.375 ± 0.051
0.216ProCys: 0.216 ± 0.014
1.861ProAsp: 1.861 ± 0.042
2.828ProGlu: 2.828 ± 0.054
2.11ProPhe: 2.11 ± 0.044
2.082ProGly: 2.082 ± 0.052
0.834ProHis: 0.834 ± 0.028
2.666ProIle: 2.666 ± 0.055
2.197ProLys: 2.197 ± 0.049
3.737ProLeu: 3.737 ± 0.064
0.878ProMet: 0.878 ± 0.031
1.606ProAsn: 1.606 ± 0.042
1.052ProPro: 1.052 ± 0.04
1.171ProGln: 1.171 ± 0.035
1.06ProArg: 1.06 ± 0.033
2.221ProSer: 2.221 ± 0.053
2.04ProThr: 2.04 ± 0.041
2.877ProVal: 2.877 ± 0.052
0.383ProTrp: 0.383 ± 0.019
1.334ProTyr: 1.334 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.505GlnAla: 3.505 ± 0.064
0.228GlnCys: 0.228 ± 0.015
1.57GlnAsp: 1.57 ± 0.036
3.315GlnGlu: 3.315 ± 0.066
1.869GlnPhe: 1.869 ± 0.047
2.311GlnGly: 2.311 ± 0.045
0.939GlnHis: 0.939 ± 0.03
2.67GlnIle: 2.67 ± 0.056
2.894GlnLys: 2.894 ± 0.059
5.132GlnLeu: 5.132 ± 0.085
1.442GlnMet: 1.442 ± 0.037
1.42GlnAsn: 1.42 ± 0.038
1.563GlnPro: 1.563 ± 0.043
2.602GlnGln: 2.602 ± 0.06
1.635GlnArg: 1.635 ± 0.044
2.488GlnSer: 2.488 ± 0.055
2.359GlnThr: 2.359 ± 0.05
2.806GlnVal: 2.806 ± 0.054
0.542GlnTrp: 0.542 ± 0.025
1.461GlnTyr: 1.461 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.646ArgAla: 2.646 ± 0.056
0.272ArgCys: 0.272 ± 0.018
1.849ArgAsp: 1.849 ± 0.043
3.217ArgGlu: 3.217 ± 0.059
1.934ArgPhe: 1.934 ± 0.044
2.347ArgGly: 2.347 ± 0.051
0.862ArgHis: 0.862 ± 0.029
2.832ArgIle: 2.832 ± 0.06
3.252ArgLys: 3.252 ± 0.059
4.271ArgLeu: 4.271 ± 0.071
1.355ArgMet: 1.355 ± 0.036
1.59ArgAsn: 1.59 ± 0.039
1.372ArgPro: 1.372 ± 0.04
1.766ArgGln: 1.766 ± 0.043
1.847ArgArg: 1.847 ± 0.048
2.249ArgSer: 2.249 ± 0.052
2.082ArgThr: 2.082 ± 0.042
2.629ArgVal: 2.629 ± 0.051
0.467ArgTrp: 0.467 ± 0.022
1.457ArgTyr: 1.457 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.875SerAla: 3.875 ± 0.06
0.464SerCys: 0.464 ± 0.022
2.619SerAsp: 2.619 ± 0.05
3.921SerGlu: 3.921 ± 0.071
3.388SerPhe: 3.388 ± 0.067
4.251SerGly: 4.251 ± 0.069
1.274SerHis: 1.274 ± 0.034
4.711SerIle: 4.711 ± 0.068
3.701SerLys: 3.701 ± 0.058
6.383SerLeu: 6.383 ± 0.092
1.784SerMet: 1.784 ± 0.04
2.195SerAsn: 2.195 ± 0.05
2.083SerPro: 2.083 ± 0.044
2.064SerGln: 2.064 ± 0.049
2.246SerArg: 2.246 ± 0.042
3.813SerSer: 3.813 ± 0.067
3.126SerThr: 3.126 ± 0.054
4.218SerVal: 4.218 ± 0.068
0.604SerTrp: 0.604 ± 0.023
2.209SerTyr: 2.209 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
4.18ThrAla: 4.18 ± 0.062
0.399ThrCys: 0.399 ± 0.021
2.582ThrAsp: 2.582 ± 0.067
3.688ThrGlu: 3.688 ± 0.06
2.625ThrPhe: 2.625 ± 0.06
4.039ThrGly: 4.039 ± 0.062
1.015ThrHis: 1.015 ± 0.03
4.621ThrIle: 4.621 ± 0.078
3.507ThrLys: 3.507 ± 0.064
5.212ThrLeu: 5.212 ± 0.069
1.395ThrMet: 1.395 ± 0.035
2.48ThrAsn: 2.48 ± 0.056
2.238ThrPro: 2.238 ± 0.046
1.452ThrGln: 1.452 ± 0.041
1.778ThrArg: 1.778 ± 0.048
3.19ThrSer: 3.19 ± 0.06
3.072ThrThr: 3.072 ± 0.054
4.311ThrVal: 4.311 ± 0.063
0.525ThrTrp: 0.525 ± 0.027
1.921ThrTyr: 1.921 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.487ValAla: 5.487 ± 0.076
0.591ValCys: 0.591 ± 0.028
3.642ValAsp: 3.642 ± 0.066
5.171ValGlu: 5.171 ± 0.07
3.234ValPhe: 3.234 ± 0.06
4.583ValGly: 4.583 ± 0.073
1.516ValHis: 1.516 ± 0.04
5.657ValIle: 5.657 ± 0.087
4.848ValLys: 4.848 ± 0.081
7.004ValLeu: 7.004 ± 0.086
2.026ValMet: 2.026 ± 0.045
2.972ValAsn: 2.972 ± 0.055
2.688ValPro: 2.688 ± 0.046
2.823ValGln: 2.823 ± 0.058
2.693ValArg: 2.693 ± 0.061
4.621ValSer: 4.621 ± 0.075
4.263ValThr: 4.263 ± 0.066
5.223ValVal: 5.223 ± 0.092
0.677ValTrp: 0.677 ± 0.027
2.29ValTyr: 2.29 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.588TrpAla: 0.588 ± 0.023
0.097TrpCys: 0.097 ± 0.013
0.477TrpAsp: 0.477 ± 0.024
0.678TrpGlu: 0.678 ± 0.029
0.499TrpPhe: 0.499 ± 0.022
0.694TrpGly: 0.694 ± 0.029
0.215TrpHis: 0.215 ± 0.015
0.769TrpIle: 0.769 ± 0.03
0.746TrpLys: 0.746 ± 0.029
1.248TrpLeu: 1.248 ± 0.04
0.4TrpMet: 0.4 ± 0.018
0.48TrpAsn: 0.48 ± 0.024
0.27TrpPro: 0.27 ± 0.019
0.418TrpGln: 0.418 ± 0.02
0.424TrpArg: 0.424 ± 0.022
0.643TrpSer: 0.643 ± 0.027
0.563TrpThr: 0.563 ± 0.025
0.676TrpVal: 0.676 ± 0.026
0.137TrpTrp: 0.137 ± 0.011
0.347TrpTyr: 0.347 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.124TyrAla: 2.124 ± 0.043
0.318TyrCys: 0.318 ± 0.021
1.824TyrAsp: 1.824 ± 0.044
2.685TyrGlu: 2.685 ± 0.06
1.788TyrPhe: 1.788 ± 0.053
2.298TyrGly: 2.298 ± 0.047
0.833TyrHis: 0.833 ± 0.028
2.372TyrIle: 2.372 ± 0.056
1.997TyrLys: 1.997 ± 0.043
3.197TyrLeu: 3.197 ± 0.063
0.884TyrMet: 0.884 ± 0.03
1.286TyrAsn: 1.286 ± 0.038
1.362TyrPro: 1.362 ± 0.037
1.613TyrGln: 1.613 ± 0.043
1.623TyrArg: 1.623 ± 0.043
2.149TyrSer: 2.149 ± 0.043
1.751TyrThr: 1.751 ± 0.04
2.307TyrVal: 2.307 ± 0.044
0.385TyrTrp: 0.385 ± 0.022
1.395TyrTyr: 1.395 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3527 proteins (1042885 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski