Amino acid dipepetide frequency for Microbacterium phage Roman

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.422AlaAla: 11.422 ± 1.075
0.467AlaCys: 0.467 ± 0.153
5.348AlaAsp: 5.348 ± 0.544
7.944AlaGlu: 7.944 ± 0.733
3.271AlaPhe: 3.271 ± 0.403
6.801AlaGly: 6.801 ± 0.963
1.713AlaHis: 1.713 ± 0.348
5.036AlaIle: 5.036 ± 0.466
4.621AlaLys: 4.621 ± 0.543
9.086AlaLeu: 9.086 ± 0.669
3.375AlaMet: 3.375 ± 0.431
4.05AlaAsn: 4.05 ± 0.47
3.219AlaPro: 3.219 ± 0.379
3.686AlaGln: 3.686 ± 0.444
6.801AlaArg: 6.801 ± 0.637
5.919AlaSer: 5.919 ± 0.67
6.438AlaThr: 6.438 ± 0.631
6.126AlaVal: 6.126 ± 0.528
2.077AlaTrp: 2.077 ± 0.307
2.44AlaTyr: 2.44 ± 0.306
0.0AlaXaa: 0.0 ± 0.0
Cys
0.156CysAla: 0.156 ± 0.079
0.052CysCys: 0.052 ± 0.055
0.519CysAsp: 0.519 ± 0.179
0.519CysGlu: 0.519 ± 0.241
0.26CysPhe: 0.26 ± 0.102
0.675CysGly: 0.675 ± 0.207
0.104CysHis: 0.104 ± 0.068
0.26CysIle: 0.26 ± 0.111
0.26CysLys: 0.26 ± 0.116
0.156CysLeu: 0.156 ± 0.091
0.052CysMet: 0.052 ± 0.061
0.208CysAsn: 0.208 ± 0.103
0.26CysPro: 0.26 ± 0.173
0.312CysGln: 0.312 ± 0.114
0.571CysArg: 0.571 ± 0.221
0.208CysSer: 0.208 ± 0.132
0.104CysThr: 0.104 ± 0.083
0.26CysVal: 0.26 ± 0.1
0.156CysTrp: 0.156 ± 0.1
0.104CysTyr: 0.104 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
6.646AspAla: 6.646 ± 0.765
0.519AspCys: 0.519 ± 0.208
4.102AspAsp: 4.102 ± 0.475
5.244AspGlu: 5.244 ± 0.62
1.973AspPhe: 1.973 ± 0.327
5.919AspGly: 5.919 ± 0.568
0.986AspHis: 0.986 ± 0.241
2.959AspIle: 2.959 ± 0.439
2.336AspLys: 2.336 ± 0.42
4.725AspLeu: 4.725 ± 0.509
1.713AspMet: 1.713 ± 0.334
2.025AspAsn: 2.025 ± 0.346
3.53AspPro: 3.53 ± 0.477
2.181AspGln: 2.181 ± 0.338
3.738AspArg: 3.738 ± 0.481
4.05AspSer: 4.05 ± 0.496
3.686AspThr: 3.686 ± 0.518
4.465AspVal: 4.465 ± 0.431
1.817AspTrp: 1.817 ± 0.276
2.648AspTyr: 2.648 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
6.646GluAla: 6.646 ± 0.647
0.415GluCys: 0.415 ± 0.149
4.569GluAsp: 4.569 ± 0.45
5.088GluGlu: 5.088 ± 0.681
2.648GluPhe: 2.648 ± 0.365
5.088GluGly: 5.088 ± 0.51
1.661GluHis: 1.661 ± 0.32
4.621GluIle: 4.621 ± 0.456
4.205GluLys: 4.205 ± 0.6
4.984GluLeu: 4.984 ± 0.526
2.077GluMet: 2.077 ± 0.382
2.959GluAsn: 2.959 ± 0.369
2.492GluPro: 2.492 ± 0.327
4.05GluGln: 4.05 ± 0.44
5.088GluArg: 5.088 ± 0.516
3.323GluSer: 3.323 ± 0.433
3.842GluThr: 3.842 ± 0.421
5.971GluVal: 5.971 ± 0.726
1.765GluTrp: 1.765 ± 0.275
3.219GluTyr: 3.219 ± 0.436
0.0GluXaa: 0.0 ± 0.0
Phe
3.271PheAla: 3.271 ± 0.367
0.104PheCys: 0.104 ± 0.066
2.752PheAsp: 2.752 ± 0.384
2.856PheGlu: 2.856 ± 0.401
0.519PhePhe: 0.519 ± 0.149
2.44PheGly: 2.44 ± 0.429
0.727PheHis: 0.727 ± 0.174
1.402PheIle: 1.402 ± 0.255
1.35PheLys: 1.35 ± 0.285
2.129PheLeu: 2.129 ± 0.357
1.194PheMet: 1.194 ± 0.227
1.454PheAsn: 1.454 ± 0.286
1.142PhePro: 1.142 ± 0.226
1.558PheGln: 1.558 ± 0.289
1.973PheArg: 1.973 ± 0.314
1.817PheSer: 1.817 ± 0.329
2.648PheThr: 2.648 ± 0.4
2.388PheVal: 2.388 ± 0.343
0.467PheTrp: 0.467 ± 0.129
1.35PheTyr: 1.35 ± 0.285
0.0PheXaa: 0.0 ± 0.0
Gly
6.594GlyAla: 6.594 ± 0.716
0.467GlyCys: 0.467 ± 0.17
5.971GlyAsp: 5.971 ± 0.514
6.074GlyGlu: 6.074 ± 0.455
3.427GlyPhe: 3.427 ± 0.529
6.697GlyGly: 6.697 ± 0.907
2.025GlyHis: 2.025 ± 0.305
4.361GlyIle: 4.361 ± 0.525
3.323GlyLys: 3.323 ± 0.498
6.023GlyLeu: 6.023 ± 0.743
1.973GlyMet: 1.973 ± 0.27
2.959GlyAsn: 2.959 ± 0.383
2.077GlyPro: 2.077 ± 0.332
2.856GlyGln: 2.856 ± 0.495
5.867GlyArg: 5.867 ± 0.541
4.309GlySer: 4.309 ± 0.413
5.607GlyThr: 5.607 ± 0.749
5.607GlyVal: 5.607 ± 0.559
2.232GlyTrp: 2.232 ± 0.414
2.907GlyTyr: 2.907 ± 0.408
0.0GlyXaa: 0.0 ± 0.0
His
1.609HisAla: 1.609 ± 0.263
0.0HisCys: 0.0 ± 0.0
1.09HisAsp: 1.09 ± 0.209
1.35HisGlu: 1.35 ± 0.245
0.467HisPhe: 0.467 ± 0.131
1.402HisGly: 1.402 ± 0.344
0.467HisHis: 0.467 ± 0.203
1.194HisIle: 1.194 ± 0.246
0.986HisLys: 0.986 ± 0.209
1.402HisLeu: 1.402 ± 0.335
0.363HisMet: 0.363 ± 0.12
0.935HisAsn: 0.935 ± 0.222
1.454HisPro: 1.454 ± 0.312
0.415HisGln: 0.415 ± 0.161
1.09HisArg: 1.09 ± 0.233
1.09HisSer: 1.09 ± 0.206
1.402HisThr: 1.402 ± 0.277
0.986HisVal: 0.986 ± 0.214
0.26HisTrp: 0.26 ± 0.096
0.675HisTyr: 0.675 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
5.244IleAla: 5.244 ± 0.564
0.312IleCys: 0.312 ± 0.152
3.738IleAsp: 3.738 ± 0.375
5.14IleGlu: 5.14 ± 0.536
1.713IlePhe: 1.713 ± 0.238
3.738IleGly: 3.738 ± 0.367
1.142IleHis: 1.142 ± 0.251
2.44IleIle: 2.44 ± 0.404
2.7IleLys: 2.7 ± 0.429
3.686IleLeu: 3.686 ± 0.414
0.779IleMet: 0.779 ± 0.188
2.232IleAsn: 2.232 ± 0.385
2.388IlePro: 2.388 ± 0.389
2.232IleGln: 2.232 ± 0.309
4.257IleArg: 4.257 ± 0.49
2.44IleSer: 2.44 ± 0.323
3.115IleThr: 3.115 ± 0.359
3.271IleVal: 3.271 ± 0.309
0.779IleTrp: 0.779 ± 0.212
1.142IleTyr: 1.142 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
5.451LysAla: 5.451 ± 0.593
0.363LysCys: 0.363 ± 0.176
3.063LysAsp: 3.063 ± 0.516
2.596LysGlu: 2.596 ± 0.368
1.921LysPhe: 1.921 ± 0.342
3.271LysGly: 3.271 ± 0.433
0.883LysHis: 0.883 ± 0.193
3.011LysIle: 3.011 ± 0.423
3.063LysLys: 3.063 ± 0.404
3.738LysLeu: 3.738 ± 0.479
1.09LysMet: 1.09 ± 0.285
1.402LysAsn: 1.402 ± 0.291
1.765LysPro: 1.765 ± 0.398
1.402LysGln: 1.402 ± 0.273
3.582LysArg: 3.582 ± 0.614
2.7LysSer: 2.7 ± 0.399
2.284LysThr: 2.284 ± 0.397
3.53LysVal: 3.53 ± 0.445
0.519LysTrp: 0.519 ± 0.165
1.09LysTyr: 1.09 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
7.476LeuAla: 7.476 ± 0.691
0.26LeuCys: 0.26 ± 0.125
5.503LeuAsp: 5.503 ± 0.574
5.036LeuGlu: 5.036 ± 0.535
2.284LeuPhe: 2.284 ± 0.339
6.542LeuGly: 6.542 ± 0.644
0.986LeuHis: 0.986 ± 0.224
3.063LeuIle: 3.063 ± 0.395
3.323LeuLys: 3.323 ± 0.366
3.582LeuLeu: 3.582 ± 0.432
1.402LeuMet: 1.402 ± 0.305
2.44LeuAsn: 2.44 ± 0.442
4.413LeuPro: 4.413 ± 0.51
2.492LeuGln: 2.492 ± 0.421
4.361LeuArg: 4.361 ± 0.52
5.296LeuSer: 5.296 ± 0.461
3.998LeuThr: 3.998 ± 0.538
4.517LeuVal: 4.517 ± 0.427
1.35LeuTrp: 1.35 ± 0.282
1.973LeuTyr: 1.973 ± 0.292
0.0LeuXaa: 0.0 ± 0.0
Met
3.167MetAla: 3.167 ± 0.346
0.052MetCys: 0.052 ± 0.055
1.35MetAsp: 1.35 ± 0.324
1.35MetGlu: 1.35 ± 0.247
0.779MetPhe: 0.779 ± 0.263
1.973MetGly: 1.973 ± 0.313
0.831MetHis: 0.831 ± 0.207
1.038MetIle: 1.038 ± 0.262
1.246MetLys: 1.246 ± 0.277
0.831MetLeu: 0.831 ± 0.218
0.571MetMet: 0.571 ± 0.184
0.935MetAsn: 0.935 ± 0.225
0.986MetPro: 0.986 ± 0.21
0.727MetGln: 0.727 ± 0.202
1.506MetArg: 1.506 ± 0.288
2.596MetSer: 2.596 ± 0.417
1.973MetThr: 1.973 ± 0.311
1.35MetVal: 1.35 ± 0.273
0.312MetTrp: 0.312 ± 0.129
0.363MetTyr: 0.363 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.271AsnAla: 3.271 ± 0.436
0.0AsnCys: 0.0 ± 0.0
2.077AsnAsp: 2.077 ± 0.344
2.284AsnGlu: 2.284 ± 0.277
1.35AsnPhe: 1.35 ± 0.248
4.621AsnGly: 4.621 ± 0.573
0.831AsnHis: 0.831 ± 0.175
1.609AsnIle: 1.609 ± 0.33
1.194AsnLys: 1.194 ± 0.297
3.375AsnLeu: 3.375 ± 0.358
0.831AsnMet: 0.831 ± 0.197
1.609AsnAsn: 1.609 ± 0.346
2.44AsnPro: 2.44 ± 0.325
1.194AsnGln: 1.194 ± 0.312
2.648AsnArg: 2.648 ± 0.416
2.648AsnSer: 2.648 ± 0.48
1.921AsnThr: 1.921 ± 0.314
2.648AsnVal: 2.648 ± 0.311
0.675AsnTrp: 0.675 ± 0.134
1.246AsnTyr: 1.246 ± 0.286
0.0AsnXaa: 0.0 ± 0.0
Pro
3.582ProAla: 3.582 ± 0.441
0.208ProCys: 0.208 ± 0.1
3.634ProAsp: 3.634 ± 0.447
4.621ProGlu: 4.621 ± 0.486
1.558ProPhe: 1.558 ± 0.304
3.946ProGly: 3.946 ± 0.422
0.779ProHis: 0.779 ± 0.205
2.025ProIle: 2.025 ± 0.307
2.284ProLys: 2.284 ± 0.37
2.752ProLeu: 2.752 ± 0.311
0.727ProMet: 0.727 ± 0.182
1.558ProAsn: 1.558 ± 0.335
1.661ProPro: 1.661 ± 0.316
1.661ProGln: 1.661 ± 0.344
1.713ProArg: 1.713 ± 0.304
2.544ProSer: 2.544 ± 0.344
2.959ProThr: 2.959 ± 0.417
3.53ProVal: 3.53 ± 0.425
0.623ProTrp: 0.623 ± 0.177
1.194ProTyr: 1.194 ± 0.22
0.0ProXaa: 0.0 ± 0.0
Gln
3.842GlnAla: 3.842 ± 0.438
0.26GlnCys: 0.26 ± 0.104
1.817GlnAsp: 1.817 ± 0.321
2.44GlnGlu: 2.44 ± 0.35
1.35GlnPhe: 1.35 ± 0.227
2.544GlnGly: 2.544 ± 0.374
0.312GlnHis: 0.312 ± 0.126
2.336GlnIle: 2.336 ± 0.371
1.661GlnLys: 1.661 ± 0.29
2.648GlnLeu: 2.648 ± 0.359
0.831GlnMet: 0.831 ± 0.199
1.35GlnAsn: 1.35 ± 0.248
1.298GlnPro: 1.298 ± 0.305
1.038GlnGln: 1.038 ± 0.178
1.973GlnArg: 1.973 ± 0.292
2.181GlnSer: 2.181 ± 0.329
1.869GlnThr: 1.869 ± 0.34
3.634GlnVal: 3.634 ± 0.373
0.831GlnTrp: 0.831 ± 0.229
0.883GlnTyr: 0.883 ± 0.23
0.0GlnXaa: 0.0 ± 0.0
Arg
6.023ArgAla: 6.023 ± 0.603
0.312ArgCys: 0.312 ± 0.157
4.361ArgAsp: 4.361 ± 0.444
4.932ArgGlu: 4.932 ± 0.666
2.129ArgPhe: 2.129 ± 0.327
4.621ArgGly: 4.621 ± 0.404
1.246ArgHis: 1.246 ± 0.266
4.205ArgIle: 4.205 ± 0.448
3.53ArgLys: 3.53 ± 0.504
4.569ArgLeu: 4.569 ± 0.509
2.025ArgMet: 2.025 ± 0.366
2.752ArgAsn: 2.752 ± 0.373
2.856ArgPro: 2.856 ± 0.382
1.973ArgGln: 1.973 ± 0.362
4.828ArgArg: 4.828 ± 0.431
3.375ArgSer: 3.375 ± 0.44
3.167ArgThr: 3.167 ± 0.421
5.192ArgVal: 5.192 ± 0.602
1.35ArgTrp: 1.35 ± 0.305
2.544ArgTyr: 2.544 ± 0.367
0.0ArgXaa: 0.0 ± 0.0
Ser
6.438SerAla: 6.438 ± 0.782
0.312SerCys: 0.312 ± 0.158
3.219SerAsp: 3.219 ± 0.488
3.686SerGlu: 3.686 ± 0.466
1.817SerPhe: 1.817 ± 0.292
5.711SerGly: 5.711 ± 0.521
1.09SerHis: 1.09 ± 0.242
3.011SerIle: 3.011 ± 0.505
3.219SerLys: 3.219 ± 0.433
4.569SerLeu: 4.569 ± 0.549
1.661SerMet: 1.661 ± 0.358
2.181SerAsn: 2.181 ± 0.497
2.232SerPro: 2.232 ± 0.316
1.609SerGln: 1.609 ± 0.262
3.634SerArg: 3.634 ± 0.396
3.998SerSer: 3.998 ± 0.694
4.102SerThr: 4.102 ± 0.48
3.738SerVal: 3.738 ± 0.466
1.454SerTrp: 1.454 ± 0.229
2.388SerTyr: 2.388 ± 0.387
0.0SerXaa: 0.0 ± 0.0
Thr
5.555ThrAla: 5.555 ± 0.644
0.363ThrCys: 0.363 ± 0.172
3.79ThrAsp: 3.79 ± 0.51
4.102ThrGlu: 4.102 ± 0.485
2.648ThrPhe: 2.648 ± 0.306
5.659ThrGly: 5.659 ± 0.644
0.883ThrHis: 0.883 ± 0.229
3.842ThrIle: 3.842 ± 0.463
2.336ThrLys: 2.336 ± 0.329
4.725ThrLeu: 4.725 ± 0.447
1.09ThrMet: 1.09 ± 0.293
2.492ThrAsn: 2.492 ± 0.383
3.738ThrPro: 3.738 ± 0.44
1.609ThrGln: 1.609 ± 0.296
3.323ThrArg: 3.323 ± 0.337
3.323ThrSer: 3.323 ± 0.457
3.738ThrThr: 3.738 ± 0.479
4.984ThrVal: 4.984 ± 0.633
1.142ThrTrp: 1.142 ± 0.243
1.661ThrTyr: 1.661 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
7.684ValAla: 7.684 ± 0.563
0.519ValCys: 0.519 ± 0.173
5.036ValAsp: 5.036 ± 0.513
5.659ValGlu: 5.659 ± 0.677
1.921ValPhe: 1.921 ± 0.333
5.451ValGly: 5.451 ± 0.457
1.142ValHis: 1.142 ± 0.215
3.738ValIle: 3.738 ± 0.483
3.79ValLys: 3.79 ± 0.402
4.517ValLeu: 4.517 ± 0.467
0.779ValMet: 0.779 ± 0.174
2.7ValAsn: 2.7 ± 0.443
3.115ValPro: 3.115 ± 0.468
2.284ValGln: 2.284 ± 0.297
5.244ValArg: 5.244 ± 0.488
4.413ValSer: 4.413 ± 0.444
5.088ValThr: 5.088 ± 0.602
4.984ValVal: 4.984 ± 0.505
1.298ValTrp: 1.298 ± 0.279
2.336ValTyr: 2.336 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
1.817TrpAla: 1.817 ± 0.278
0.052TrpCys: 0.052 ± 0.048
1.506TrpAsp: 1.506 ± 0.267
1.609TrpGlu: 1.609 ± 0.36
0.571TrpPhe: 0.571 ± 0.174
1.973TrpGly: 1.973 ± 0.315
0.26TrpHis: 0.26 ± 0.124
0.986TrpIle: 0.986 ± 0.216
0.312TrpLys: 0.312 ± 0.129
1.038TrpLeu: 1.038 ± 0.185
0.623TrpMet: 0.623 ± 0.197
0.986TrpAsn: 0.986 ± 0.184
0.831TrpPro: 0.831 ± 0.207
0.571TrpGln: 0.571 ± 0.217
1.869TrpArg: 1.869 ± 0.399
1.454TrpSer: 1.454 ± 0.287
0.883TrpThr: 0.883 ± 0.243
1.765TrpVal: 1.765 ± 0.333
0.623TrpTrp: 0.623 ± 0.165
0.623TrpTyr: 0.623 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.946TyrAla: 3.946 ± 0.463
0.156TyrCys: 0.156 ± 0.081
1.817TyrAsp: 1.817 ± 0.397
2.181TyrGlu: 2.181 ± 0.359
0.935TyrPhe: 0.935 ± 0.223
2.284TyrGly: 2.284 ± 0.297
0.571TyrHis: 0.571 ± 0.135
1.35TyrIle: 1.35 ± 0.29
0.986TyrLys: 0.986 ± 0.229
1.765TyrLeu: 1.765 ± 0.433
0.727TyrMet: 0.727 ± 0.186
1.246TyrAsn: 1.246 ± 0.2
1.661TyrPro: 1.661 ± 0.255
1.246TyrGln: 1.246 ± 0.279
1.973TyrArg: 1.973 ± 0.348
2.388TyrSer: 2.388 ± 0.35
2.181TyrThr: 2.181 ± 0.283
2.752TyrVal: 2.752 ± 0.353
0.623TyrTrp: 0.623 ± 0.175
0.986TyrTyr: 0.986 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 116 proteins (19262 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski