Muntiacus reevesi (Reeves muntjac) (Cervus reevesi)
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 25423 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N3XL44|A0A5N3XL44_MUNRE Uncharacterized protein OS=Muntiacus reevesi OX=9886 GN=FD755_013088 PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 5.06 SDD4 pKa = 3.49 EE5 pKa = 4.62 GYY7 pKa = 10.94 NYY9 pKa = 10.32 EE10 pKa = 3.8 FDD12 pKa = 3.84 EE13 pKa = 5.7 DD14 pKa = 4.45 EE15 pKa = 4.49 EE16 pKa = 4.84 CSEE19 pKa = 4.14 EE20 pKa = 4.25 DD21 pKa = 3.14 SGAEE25 pKa = 3.96 EE26 pKa = 5.43 EE27 pKa = 4.47 EE28 pKa = 5.62 DD29 pKa = 4.29 EE30 pKa = 6.38 DD31 pKa = 6.15 DD32 pKa = 6.53 DD33 pKa = 6.32 EE34 pKa = 7.2 PDD36 pKa = 5.44 DD37 pKa = 5.75 DD38 pKa = 5.33 NLDD41 pKa = 3.84 LGEE44 pKa = 4.35 VEE46 pKa = 4.89 LVEE49 pKa = 4.7 PGLGVGGEE57 pKa = 4.21 RR58 pKa = 11.84 DD59 pKa = 3.44 GLLCGEE65 pKa = 4.36 TGGGGGSALGPGGGGGGGGGGGGPGHH91 pKa = 5.93 EE92 pKa = 4.15 QEE94 pKa = 3.32 EE95 pKa = 4.9 DD96 pKa = 3.22 YY97 pKa = 10.77 RR98 pKa = 11.84 YY99 pKa = 10.14 EE100 pKa = 4.0 VLTAEE105 pKa = 5.1 QILQHH110 pKa = 5.41 MVEE113 pKa = 5.29 CIRR116 pKa = 11.84 EE117 pKa = 4.19 VNEE120 pKa = 4.42 VIQPPQTSLAGNCCGSLGTVSFLWPQEE147 pKa = 3.73 MSFLCKK153 pKa = 10.28 KK154 pKa = 10.55 FIFRR158 pKa = 11.84 NISRR162 pKa = 11.84 SKK164 pKa = 10.94 DD165 pKa = 3.21 RR166 pKa = 11.84 IYY168 pKa = 10.6 PQILFLEE175 pKa = 4.51 MVYY178 pKa = 9.62 TGQMMQQ184 pKa = 3.19
Molecular weight: 19.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.516
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.554
Rodwell 3.541
Grimsley 3.427
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 3.935
Thurlkill 3.554
EMBOSS 3.567
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.656
IPC2_peptide 3.808
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A5N3XT15|A0A5N3XT15_MUNRE Uncharacterized protein OS=Muntiacus reevesi OX=9886 GN=FD755_010869 PE=4 SV=1
MM1 pKa = 7.74 APPIRR6 pKa = 11.84 TRR8 pKa = 11.84 PSFHH12 pKa = 6.97 LSLSRR17 pKa = 11.84 QEE19 pKa = 4.71 ASISLLSFSIRR30 pKa = 11.84 GGRR33 pKa = 11.84 PRR35 pKa = 11.84 PQGPPRR41 pKa = 11.84 PRR43 pKa = 11.84 GPPRR47 pKa = 11.84 PWGSQPRR54 pKa = 11.84 GPPRR58 pKa = 11.84 PRR60 pKa = 11.84 RR61 pKa = 11.84 HH62 pKa = 5.36 PQPWGPPQPRR72 pKa = 11.84 GPPRR76 pKa = 11.84 PRR78 pKa = 11.84 GHH80 pKa = 7.28 PRR82 pKa = 11.84 PQGPPQPRR90 pKa = 11.84 GPPRR94 pKa = 11.84 PRR96 pKa = 11.84 GHH98 pKa = 6.09 PQPWGSRR105 pKa = 11.84 PWGPPRR111 pKa = 11.84 PRR113 pKa = 11.84 GHH115 pKa = 7.28 PRR117 pKa = 11.84 PQGPPRR123 pKa = 11.84 PRR125 pKa = 11.84 GYY127 pKa = 9.03 PQPWGSRR134 pKa = 11.84 PWGPPRR140 pKa = 11.84 PRR142 pKa = 11.84 GHH144 pKa = 7.28 PRR146 pKa = 11.84 PQGPPRR152 pKa = 11.84 PRR154 pKa = 11.84 GHH156 pKa = 6.28 PQPWGPPRR164 pKa = 11.84 PRR166 pKa = 11.84 GSPQPRR172 pKa = 11.84 GHH174 pKa = 6.67 PRR176 pKa = 11.84 PRR178 pKa = 11.84 GHH180 pKa = 6.49 SS181 pKa = 3.29
Molecular weight: 20.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.374
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.559
Grimsley 13.1
Solomon 13.554
Lehninger 13.451
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.266
IPC_peptide 13.554
IPC2_peptide 12.544
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
25423
0
25423
11766880
25
32191
462.8
51.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.991 ± 0.021
2.366 ± 0.015
4.618 ± 0.01
6.814 ± 0.022
3.825 ± 0.012
6.589 ± 0.023
2.662 ± 0.012
4.427 ± 0.017
5.658 ± 0.02
10.146 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.008
3.481 ± 0.011
6.339 ± 0.028
4.595 ± 0.014
5.713 ± 0.014
8.285 ± 0.023
5.293 ± 0.018
6.062 ± 0.016
1.27 ± 0.006
2.686 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here