Simian foamy virus (isolate chimpanzee) (SFVcpz)
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q87043|BET_SFVCP Protein Bet OS=Simian foamy virus (isolate chimpanzee) OX=298339 GN=bet PE=3 SV=2
MM1 pKa = 8.15 DD2 pKa = 5.26 SYY4 pKa = 11.65 QEE6 pKa = 4.13 EE7 pKa = 4.57 EE8 pKa = 4.45 PVASTSGLQDD18 pKa = 3.54 LQTLSEE24 pKa = 4.26 LVGPEE29 pKa = 3.67 NAGEE33 pKa = 4.19 GDD35 pKa = 3.88 LVIAEE40 pKa = 4.4 EE41 pKa = 4.36 PEE43 pKa = 4.05 EE44 pKa = 4.12 NPRR47 pKa = 11.84 RR48 pKa = 11.84 PRR50 pKa = 11.84 RR51 pKa = 11.84 YY52 pKa = 7.27 TKK54 pKa = 10.46 RR55 pKa = 11.84 DD56 pKa = 3.58 VKK58 pKa = 10.62 CVSYY62 pKa = 10.26 HH63 pKa = 6.49 AYY65 pKa = 9.84 KK66 pKa = 10.5 EE67 pKa = 4.7 LEE69 pKa = 4.62 DD70 pKa = 3.87 KK71 pKa = 10.9 HH72 pKa = 5.92 PHH74 pKa = 6.57 HH75 pKa = 7.33 IKK77 pKa = 10.4 LQDD80 pKa = 3.88 WIPKK84 pKa = 8.09 PEE86 pKa = 4.05 EE87 pKa = 3.78 MSKK90 pKa = 10.64 SICKK94 pKa = 10.12 RR95 pKa = 11.84 LILCGLYY102 pKa = 10.34 SGEE105 pKa = 4.21 KK106 pKa = 9.97 ARR108 pKa = 11.84 EE109 pKa = 3.79 ILKK112 pKa = 10.73 KK113 pKa = 10.03 PFTVSWEE120 pKa = 4.03 QSEE123 pKa = 4.6 TNPDD127 pKa = 3.82 CFIVSYY133 pKa = 9.84 TCIFCDD139 pKa = 4.74 AVIHH143 pKa = 7.18 DD144 pKa = 4.8 PMPVVWDD151 pKa = 3.82 SEE153 pKa = 4.45 VEE155 pKa = 3.58 IWVKK159 pKa = 10.29 YY160 pKa = 9.93 KK161 pKa = 10.12 PLRR164 pKa = 11.84 GIVGSAVFIMEE175 pKa = 4.57 KK176 pKa = 9.77 HH177 pKa = 5.6 QKK179 pKa = 9.38 NCSLVKK185 pKa = 10.32 PSTSCPEE192 pKa = 3.85 GPKK195 pKa = 9.57 PRR197 pKa = 11.84 RR198 pKa = 11.84 RR199 pKa = 11.84 HH200 pKa = 6.23 DD201 pKa = 3.31 PVLRR205 pKa = 11.84 CDD207 pKa = 3.51 MFEE210 pKa = 3.97 KK211 pKa = 10.12 HH212 pKa = 6.29 HH213 pKa = 6.51 KK214 pKa = 9.38 PRR216 pKa = 11.84 PKK218 pKa = 9.97 RR219 pKa = 11.84 SRR221 pKa = 11.84 KK222 pKa = 9.42 RR223 pKa = 11.84 SIDD226 pKa = 3.52 HH227 pKa = 6.58 EE228 pKa = 4.81 SCASSGDD235 pKa = 3.68 TVANEE240 pKa = 4.06 SGPLCTNTFWTPGPVLQGLLGEE262 pKa = 4.63 SSNLPDD268 pKa = 5.18 LEE270 pKa = 4.3 VHH272 pKa = 5.83 MSGGPFWKK280 pKa = 9.88 EE281 pKa = 3.62 VYY283 pKa = 10.13 GDD285 pKa = 4.47 SILGPPSGSGEE296 pKa = 4.05 HH297 pKa = 6.54 SVLL300 pKa = 4.05
Molecular weight: 33.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.689
IPC2_protein 5.677
IPC_protein 5.703
Toseland 6.046
ProMoST 6.046
Dawson 5.957
Bjellqvist 5.97
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.173
Solomon 5.957
Lehninger 5.944
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.326
Patrickios 4.088
IPC_peptide 5.97
IPC2_peptide 6.313
IPC2.peptide.svr19 6.286
Protein with the highest isoelectric point:
>sp|Q87041|ENV_SFVCP Envelope glycoprotein gp130 OS=Simian foamy virus (isolate chimpanzee) OX=298339 GN=env PE=1 SV=1
MM1 pKa = 7.58 NPLQLLQPLPAEE13 pKa = 4.36 VKK15 pKa = 8.81 GTKK18 pKa = 10.06 LLAHH22 pKa = 7.26 WDD24 pKa = 3.38 SGATITCIPEE34 pKa = 3.91 SFLEE38 pKa = 4.26 DD39 pKa = 3.61 EE40 pKa = 4.51 QPIKK44 pKa = 9.74 QTLIKK49 pKa = 9.02 TIHH52 pKa = 6.5 GEE54 pKa = 3.94 KK55 pKa = 9.11 QQNVYY60 pKa = 11.11 YY61 pKa = 9.72 LTFKK65 pKa = 10.78 VKK67 pKa = 9.58 GRR69 pKa = 11.84 KK70 pKa = 8.78 VEE72 pKa = 4.14 AEE74 pKa = 4.43 VIASPYY80 pKa = 10.53 EE81 pKa = 4.08 YY82 pKa = 10.19 ILLSPTDD89 pKa = 4.22 VPWLTQQPLQLTILVPLQEE108 pKa = 4.3 YY109 pKa = 9.4 QDD111 pKa = 4.48 RR112 pKa = 11.84 ILNKK116 pKa = 8.67 TALPEE121 pKa = 3.99 EE122 pKa = 4.49 QKK124 pKa = 10.35 QQLKK128 pKa = 10.88 ALFTKK133 pKa = 10.53 YY134 pKa = 10.87 DD135 pKa = 3.77 NLWQHH140 pKa = 6.27 WEE142 pKa = 3.98 NQVGHH147 pKa = 6.44 RR148 pKa = 11.84 KK149 pKa = 8.88 IRR151 pKa = 11.84 PHH153 pKa = 6.76 NIATGDD159 pKa = 3.71 YY160 pKa = 9.61 PPRR163 pKa = 11.84 PQKK166 pKa = 10.35 QYY168 pKa = 10.69 PINPKK173 pKa = 9.94 AKK175 pKa = 9.26 PSIQIVIDD183 pKa = 4.01 DD184 pKa = 4.33 LLKK187 pKa = 10.97 QGVLTPQNSTMNTPVYY203 pKa = 9.51 PVPKK207 pKa = 9.66 PDD209 pKa = 3.26 GRR211 pKa = 11.84 WRR213 pKa = 11.84 MVLDD217 pKa = 3.37 YY218 pKa = 11.29 RR219 pKa = 11.84 EE220 pKa = 4.13 VNKK223 pKa = 9.63 TIPLTAAQNQHH234 pKa = 5.59 SAGILATIVRR244 pKa = 11.84 QKK246 pKa = 11.38 YY247 pKa = 7.57 KK248 pKa = 8.69 TTLDD252 pKa = 3.55 LANGFWAHH260 pKa = 7.52 PITPDD265 pKa = 4.31 SYY267 pKa = 10.69 WLTAFTWQGKK277 pKa = 6.31 QYY279 pKa = 10.3 CWTRR283 pKa = 11.84 LPQGFLNSPALFTADD298 pKa = 4.28 AVDD301 pKa = 4.11 LLKK304 pKa = 10.55 EE305 pKa = 4.11 VPNVQVYY312 pKa = 10.52 VDD314 pKa = 5.3 DD315 pKa = 5.29 IYY317 pKa = 11.41 LSHH320 pKa = 7.73 DD321 pKa = 3.69 NPHH324 pKa = 5.64 EE325 pKa = 4.83 HH326 pKa = 6.19 IQQLEE331 pKa = 4.22 KK332 pKa = 10.97 VFQILLQAGYY342 pKa = 10.34 VVSLKK347 pKa = 10.48 KK348 pKa = 10.57 SEE350 pKa = 3.66 IGQRR354 pKa = 11.84 TVEE357 pKa = 3.87 FLGFNITKK365 pKa = 9.84 EE366 pKa = 4.23 GRR368 pKa = 11.84 GLTDD372 pKa = 3.13 TFKK375 pKa = 10.78 TKK377 pKa = 10.49 LLNVTPPKK385 pKa = 10.22 DD386 pKa = 3.68 LKK388 pKa = 10.75 QLQSILGLLNFARR401 pKa = 11.84 NFIPNFAEE409 pKa = 4.98 LVQTLYY415 pKa = 11.6 NLIASSKK422 pKa = 9.77 GKK424 pKa = 10.09 YY425 pKa = 9.47 IEE427 pKa = 4.01 WTEE430 pKa = 4.81 DD431 pKa = 2.9 NTKK434 pKa = 10.32 QLNKK438 pKa = 10.1 VIEE441 pKa = 4.49 ALNTASNLEE450 pKa = 3.94 EE451 pKa = 4.21 RR452 pKa = 11.84 LPDD455 pKa = 3.22 QRR457 pKa = 11.84 LVIKK461 pKa = 11.0 VNTSPSAGYY470 pKa = 9.01 VRR472 pKa = 11.84 YY473 pKa = 10.31 YY474 pKa = 10.82 NEE476 pKa = 4.28 SGKK479 pKa = 10.57 KK480 pKa = 9.46 PIMYY484 pKa = 10.05 LNYY487 pKa = 9.51 VFSKK491 pKa = 11.39 AEE493 pKa = 4.14 LKK495 pKa = 10.77 FSMLEE500 pKa = 3.85 KK501 pKa = 10.79 LLTTMHH507 pKa = 6.98 KK508 pKa = 10.82 ALIKK512 pKa = 11.07 AMDD515 pKa = 3.95 LAMGQEE521 pKa = 3.93 ILVYY525 pKa = 10.46 SPIVSMTKK533 pKa = 9.42 IQKK536 pKa = 8.42 TPLPEE541 pKa = 4.38 RR542 pKa = 11.84 KK543 pKa = 9.23 ALPIRR548 pKa = 11.84 WITWMTYY555 pKa = 10.7 LEE557 pKa = 4.43 DD558 pKa = 3.7 PRR560 pKa = 11.84 IQFHH564 pKa = 5.99 YY565 pKa = 10.92 DD566 pKa = 2.7 KK567 pKa = 10.5 TLPEE571 pKa = 4.67 LKK573 pKa = 10.03 HH574 pKa = 7.56 IPDD577 pKa = 4.49 VYY579 pKa = 10.31 TSSIPPLKK587 pKa = 10.55 HH588 pKa = 5.65 PSQYY592 pKa = 11.05 EE593 pKa = 4.21 GVFCTDD599 pKa = 2.76 GSAIKK604 pKa = 10.81 SPDD607 pKa = 3.28 PTKK610 pKa = 11.16 SNNAGMGIVHH620 pKa = 7.8 AIYY623 pKa = 10.46 NPEE626 pKa = 3.9 YY627 pKa = 10.58 KK628 pKa = 9.94 ILNQWSIPLGHH639 pKa = 6.84 HH640 pKa = 5.71 TAQMAEE646 pKa = 3.68 IAAVEE651 pKa = 4.32 FACKK655 pKa = 9.8 KK656 pKa = 9.41 ALKK659 pKa = 10.53 VPGPVLVITDD669 pKa = 3.76 SFYY672 pKa = 11.32 VAEE675 pKa = 4.48 SANKK679 pKa = 8.91 EE680 pKa = 3.85 LPYY683 pKa = 9.92 WKK685 pKa = 10.68 SNGFVNNKK693 pKa = 9.6 KK694 pKa = 10.33 EE695 pKa = 4.04 PLKK698 pKa = 10.84 HH699 pKa = 5.17 ISKK702 pKa = 9.07 WKK704 pKa = 10.54 SIAEE708 pKa = 4.14 CLSIKK713 pKa = 10.25 PDD715 pKa = 2.96 ITIQHH720 pKa = 6.25 EE721 pKa = 4.58 KK722 pKa = 9.8 GHH724 pKa = 5.53 QPINTSIHH732 pKa = 5.56 TEE734 pKa = 4.09 GNALADD740 pKa = 3.72 KK741 pKa = 10.67 LATQGSYY748 pKa = 9.31 VVNCNTKK755 pKa = 10.23 KK756 pKa = 10.43 PNLDD760 pKa = 3.67 AEE762 pKa = 4.72 LDD764 pKa = 3.59 QLLQGNNVKK773 pKa = 10.3 GYY775 pKa = 8.0 PKK777 pKa = 10.18 QYY779 pKa = 10.25 TYY781 pKa = 11.36 YY782 pKa = 11.02 LEE784 pKa = 4.6 DD785 pKa = 3.48 GKK787 pKa = 11.54 VKK789 pKa = 10.2 VSRR792 pKa = 11.84 PEE794 pKa = 3.75 GVKK797 pKa = 10.18 IIPPQSDD804 pKa = 3.12 RR805 pKa = 11.84 QKK807 pKa = 10.81 IVLQAHH813 pKa = 6.08 NLAHH817 pKa = 6.87 TGRR820 pKa = 11.84 EE821 pKa = 3.94 ATLLKK826 pKa = 10.2 IANLYY831 pKa = 6.99 WWPNMRR837 pKa = 11.84 KK838 pKa = 9.79 DD839 pKa = 3.63 VVKK842 pKa = 10.75 QLGRR846 pKa = 11.84 CKK848 pKa = 10.27 QCLITNASNKK858 pKa = 8.96 TSGPILRR865 pKa = 11.84 PDD867 pKa = 3.88 RR868 pKa = 11.84 PQKK871 pKa = 10.66 PFDD874 pKa = 4.08 KK875 pKa = 10.93 FFIDD879 pKa = 4.29 YY880 pKa = 10.24 IGPLPPSQGYY890 pKa = 10.06 LYY892 pKa = 11.05 VLVIVDD898 pKa = 4.14 GMTGFTWLYY907 pKa = 5.78 PTKK910 pKa = 10.57 APSTSATVKK919 pKa = 10.31 SLNVLTSIAIPKK931 pKa = 9.28 VIHH934 pKa = 6.65 SDD936 pKa = 2.79 QGAAFTSSTFAEE948 pKa = 4.41 WAKK951 pKa = 10.58 EE952 pKa = 3.85 RR953 pKa = 11.84 GIHH956 pKa = 6.76 LEE958 pKa = 3.71 FSTPYY963 pKa = 10.08 HH964 pKa = 5.98 PQSSGKK970 pKa = 8.3 VEE972 pKa = 4.3 RR973 pKa = 11.84 KK974 pKa = 10.0 NSDD977 pKa = 2.61 IKK979 pKa = 11.08 RR980 pKa = 11.84 LLTKK984 pKa = 10.58 LLVGRR989 pKa = 11.84 PTKK992 pKa = 10.03 WYY994 pKa = 9.91 DD995 pKa = 3.56 LLPVVQLALNNTYY1008 pKa = 10.92 SPVLKK1013 pKa = 9.14 YY1014 pKa = 9.21 TPHH1017 pKa = 6.16 QLLFGIDD1024 pKa = 3.66 SNTPFANQDD1033 pKa = 3.49 TLDD1036 pKa = 3.66 LTRR1039 pKa = 11.84 EE1040 pKa = 4.18 EE1041 pKa = 4.48 EE1042 pKa = 4.13 LSLLQEE1048 pKa = 4.32 IRR1050 pKa = 11.84 ASLYY1054 pKa = 10.43 QPSTPPASSRR1064 pKa = 11.84 SWSPVVGQLVQEE1076 pKa = 4.66 RR1077 pKa = 11.84 VARR1080 pKa = 11.84 PASLRR1085 pKa = 11.84 PRR1087 pKa = 11.84 WHH1089 pKa = 6.64 KK1090 pKa = 10.31 PSTVLEE1096 pKa = 4.18 VLNPRR1101 pKa = 11.84 TVVILDD1107 pKa = 3.34 HH1108 pKa = 6.8 LGNNRR1113 pKa = 11.84 TVSIDD1118 pKa = 3.34 NLKK1121 pKa = 8.75 PTSHH1125 pKa = 6.65 QNGTTNDD1132 pKa = 3.86 TATMDD1137 pKa = 3.53 HH1138 pKa = 6.93 LEE1140 pKa = 4.06 QNEE1143 pKa = 4.06 QSSS1146 pKa = 3.61
Molecular weight: 129.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.584
IPC2_protein 8.551
IPC_protein 8.404
Toseland 9.107
ProMoST 8.946
Dawson 9.428
Bjellqvist 9.18
Wikipedia 9.604
Rodwell 9.677
Grimsley 9.502
Solomon 9.443
Lehninger 9.399
Nozaki 9.238
DTASelect 9.136
Thurlkill 9.268
EMBOSS 9.56
Sillero 9.385
Patrickios 4.596
IPC_peptide 9.443
IPC2_peptide 7.702
IPC2.peptide.svr19 7.693
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
1
6
3941
300
1146
656.8
74.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.633 ± 0.341
1.675 ± 0.502
4.517 ± 0.384
6.09 ± 0.473
2.512 ± 0.183
6.344 ± 1.077
2.639 ± 0.196
5.684 ± 0.57
6.064 ± 1.077
8.932 ± 0.829
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.827 ± 0.188
4.796 ± 0.385
7.435 ± 0.764
5.785 ± 0.403
5.151 ± 0.899
6.623 ± 0.587
5.963 ± 0.456
5.938 ± 0.463
2.005 ± 0.295
4.39 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here