Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium; Halanaerobium saccharolyticum; Halanaerobium saccharolyticum subsp. saccharolyticum

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2662 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5EGC9|M5EGC9_9FIRM Late competence protein ComEA DNA receptor OS=Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643 OX=1293054 GN=HSACCH_02065 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.49KK3 pKa = 9.03LTITIALMLVVALAMPAFGASFSDD27 pKa = 3.87VPSDD31 pKa = 2.75HH32 pKa = 6.79WAYY35 pKa = 11.04NAINKK40 pKa = 8.94LVAAGIVEE48 pKa = 4.82GYY50 pKa = 9.83PDD52 pKa = 4.23GEE54 pKa = 4.41YY55 pKa = 10.38KK56 pKa = 10.64GQQSMTRR63 pKa = 11.84YY64 pKa = 8.92EE65 pKa = 4.09MAVMVSRR72 pKa = 11.84ALDD75 pKa = 3.94NIVDD79 pKa = 3.7EE80 pKa = 4.47MEE82 pKa = 4.32AMGEE86 pKa = 4.14GLTTGQAEE94 pKa = 4.49DD95 pKa = 3.17VTAIVKK101 pKa = 10.45SLMEE105 pKa = 4.62KK106 pKa = 8.88NTNDD110 pKa = 3.79EE111 pKa = 4.3LSDD114 pKa = 3.63AQAEE118 pKa = 4.25EE119 pKa = 4.16VADD122 pKa = 3.71IVDD125 pKa = 3.58ALTFEE130 pKa = 4.75LRR132 pKa = 11.84SEE134 pKa = 4.25LKK136 pKa = 10.78VLGAEE141 pKa = 3.96VDD143 pKa = 3.87TLGKK147 pKa = 10.44DD148 pKa = 3.53VDD150 pKa = 3.77EE151 pKa = 4.87LAAKK155 pKa = 9.63VEE157 pKa = 3.99AMEE160 pKa = 4.24VPEE163 pKa = 4.67DD164 pKa = 3.97NIEE167 pKa = 4.06FGMDD171 pKa = 2.95VTTTFEE177 pKa = 3.86AASYY181 pKa = 11.17GEE183 pKa = 4.31DD184 pKa = 3.45AAEE187 pKa = 3.93EE188 pKa = 5.43GYY190 pKa = 9.23TINLLEE196 pKa = 5.59DD197 pKa = 3.85GDD199 pKa = 5.37AIDD202 pKa = 5.26EE203 pKa = 4.77SFTSDD208 pKa = 3.41PDD210 pKa = 3.3EE211 pKa = 4.56FTSKK215 pKa = 10.64KK216 pKa = 10.78AFFQEE221 pKa = 3.54YY222 pKa = 10.16DD223 pKa = 3.54FNINGMLGDD232 pKa = 3.72ATFNLDD238 pKa = 2.52VDD240 pKa = 4.61TIANVFTEE248 pKa = 4.25EE249 pKa = 3.97DD250 pKa = 3.43HH251 pKa = 6.27VANAVSEE258 pKa = 4.4TNQNDD263 pKa = 3.17FMMDD267 pKa = 3.25SALLEE272 pKa = 4.04VSYY275 pKa = 11.43NNYY278 pKa = 9.94SFQIGDD284 pKa = 3.77LNDD287 pKa = 3.54YY288 pKa = 10.91SVAPYY293 pKa = 10.57FNDD296 pKa = 3.24EE297 pKa = 4.06EE298 pKa = 4.19DD299 pKa = 3.88RR300 pKa = 11.84EE301 pKa = 4.62GIEE304 pKa = 3.96MTTSYY309 pKa = 10.19MDD311 pKa = 3.66NDD313 pKa = 2.93IKK315 pKa = 10.48TFVLGADD322 pKa = 3.43VSEE325 pKa = 4.52DD326 pKa = 3.5TFGTTTVDD334 pKa = 2.69SDD336 pKa = 4.34ANDD339 pKa = 3.27EE340 pKa = 4.17EE341 pKa = 5.58YY342 pKa = 11.3YY343 pKa = 10.62GVEE346 pKa = 4.11VARR349 pKa = 11.84DD350 pKa = 3.58MEE352 pKa = 4.69FGRR355 pKa = 11.84VTGKK359 pKa = 10.49LYY361 pKa = 10.35HH362 pKa = 6.8ARR364 pKa = 11.84DD365 pKa = 3.21IDD367 pKa = 3.81FDD369 pKa = 4.07GFNMAEE375 pKa = 4.14YY376 pKa = 10.68AVTAEE381 pKa = 4.27KK382 pKa = 11.24NEE384 pKa = 4.16TTILAAQIEE393 pKa = 4.76DD394 pKa = 3.83VVITDD399 pKa = 5.36AISMGAEE406 pKa = 3.95VAFSDD411 pKa = 4.11WEE413 pKa = 3.9RR414 pKa = 11.84DD415 pKa = 3.07AYY417 pKa = 7.82TTYY420 pKa = 11.33DD421 pKa = 3.29SDD423 pKa = 3.88ASATEE428 pKa = 4.17AVAADD433 pKa = 4.78DD434 pKa = 5.77DD435 pKa = 4.69SDD437 pKa = 4.19IYY439 pKa = 11.47FNVNGEE445 pKa = 4.22FVATEE450 pKa = 4.32EE451 pKa = 4.33LTLDD455 pKa = 3.45ATIEE459 pKa = 4.33TVGEE463 pKa = 4.32DD464 pKa = 3.97FVGPYY469 pKa = 10.29NDD471 pKa = 4.67LEE473 pKa = 4.24EE474 pKa = 5.74ASDD477 pKa = 3.84YY478 pKa = 12.04DD479 pKa = 3.86MFNVGAEE486 pKa = 4.1YY487 pKa = 11.01VLNEE491 pKa = 3.99NNTVTGAYY499 pKa = 9.14TLVDD503 pKa = 3.63HH504 pKa = 7.02NEE506 pKa = 4.27PEE508 pKa = 4.42DD509 pKa = 3.86KK510 pKa = 10.53STVEE514 pKa = 4.18LGLEE518 pKa = 3.89NVYY521 pKa = 11.43GDD523 pKa = 3.8FTNNASVEE531 pKa = 4.19FVMNDD536 pKa = 3.74DD537 pKa = 3.71YY538 pKa = 11.87TDD540 pKa = 4.24DD541 pKa = 4.48YY542 pKa = 10.47EE543 pKa = 4.49TMIIVLGTEE552 pKa = 4.32YY553 pKa = 10.95AWDD556 pKa = 3.55EE557 pKa = 4.25TLTLGAEE564 pKa = 4.41LTNKK568 pKa = 9.28TKK570 pKa = 11.08DD571 pKa = 3.41EE572 pKa = 4.84AGTDD576 pKa = 3.79AITYY580 pKa = 10.08NYY582 pKa = 8.15LTAFADD588 pKa = 4.82KK589 pKa = 10.42EE590 pKa = 4.07LADD593 pKa = 4.27NITWNTEE600 pKa = 3.24AFFIDD605 pKa = 4.56GEE607 pKa = 4.15LDD609 pKa = 3.87EE610 pKa = 5.65INEE613 pKa = 4.23GQGNGMTTSLSVSFF627 pKa = 4.81

Molecular weight: 69.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5EF68|M5EF68_9FIRM Uncharacterized protein OS=Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643 OX=1293054 GN=HSACCH_01585 PE=4 SV=1
MM1 pKa = 7.4NSKK4 pKa = 10.32KK5 pKa = 10.81GIIFTGGIIGILGGLLVVFGNPANMGICVACFIRR39 pKa = 11.84DD40 pKa = 3.12IAGAVGLHH48 pKa = 6.23RR49 pKa = 11.84AAVVQNIRR57 pKa = 11.84PEE59 pKa = 4.23IIGFILGAFLIARR72 pKa = 11.84STGEE76 pKa = 3.96FKK78 pKa = 11.21VKK80 pKa = 10.4GGSSPITRR88 pKa = 11.84FVIAMFVMIGALVFLGCPLRR108 pKa = 11.84MVLRR112 pKa = 11.84LAGGDD117 pKa = 3.23WNAIAGIIGFVLGVLAGSQLLKK139 pKa = 10.82KK140 pKa = 10.46GFTLGRR146 pKa = 11.84SYY148 pKa = 10.99KK149 pKa = 9.67INQINGYY156 pKa = 8.6ILPIIAVVLLIFRR169 pKa = 11.84IVRR172 pKa = 11.84PSFIIFSEE180 pKa = 4.1KK181 pKa = 10.78GPGASYY187 pKa = 11.02AFWAIALAAGIIIGILAQRR206 pKa = 11.84SRR208 pKa = 11.84ICMAGGIRR216 pKa = 11.84DD217 pKa = 4.16LYY219 pKa = 10.51LIKK222 pKa = 10.36DD223 pKa = 3.44SHH225 pKa = 7.26LITGFISIFIFAFIVNLIFGNFNPGFAGQPAAHH258 pKa = 6.33SQWLWNLLGMFLTGLGSVMLGGCPLRR284 pKa = 11.84QTILAGEE291 pKa = 4.69GNTDD295 pKa = 3.22SAVAFVGYY303 pKa = 10.07LVGAAISHH311 pKa = 5.82NFGLAGSGSGVSTNGRR327 pKa = 11.84IAVISGIVILFIIAYY342 pKa = 10.21SSIQFNKK349 pKa = 10.01KK350 pKa = 9.9AVV352 pKa = 3.22

Molecular weight: 37.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2662

0

2662

861534

37

4311

323.6

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.134 ± 0.052

0.616 ± 0.014

5.776 ± 0.04

8.326 ± 0.06

4.494 ± 0.036

6.405 ± 0.049

1.459 ± 0.017

8.987 ± 0.049

8.286 ± 0.057

9.925 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.024

5.722 ± 0.058

3.036 ± 0.025

3.038 ± 0.029

3.549 ± 0.033

6.02 ± 0.048

4.333 ± 0.028

6.034 ± 0.042

0.729 ± 0.015

3.715 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski