Roseivivax marinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseivivax

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4HNM1|W4HNM1_9RHOB Alpha-E domain-containing protein OS=Roseivivax marinus OX=1379903 GN=ATO8_00520 PE=4 SV=1
MM1 pKa = 7.22TVDD4 pKa = 3.67IYY6 pKa = 11.58APTSSDD12 pKa = 3.49SLSLTEE18 pKa = 4.29LDD20 pKa = 4.34LYY22 pKa = 10.76HH23 pKa = 7.01MIMDD27 pKa = 3.7YY28 pKa = 10.64RR29 pKa = 11.84AASGLPAIPLSQSLTTVAGRR49 pKa = 11.84HH50 pKa = 5.04TLDD53 pKa = 3.32SYY55 pKa = 12.0HH56 pKa = 6.93NIEE59 pKa = 4.19AAGVSLPSGYY69 pKa = 10.5SSPSWIHH76 pKa = 5.42SWSDD80 pKa = 2.94AYY82 pKa = 11.61YY83 pKa = 10.37DD84 pKa = 4.24GSDD87 pKa = 3.41SSVMRR92 pKa = 11.84DD93 pKa = 3.12APSRR97 pKa = 11.84LGTGYY102 pKa = 9.85PGGGYY107 pKa = 9.74EE108 pKa = 4.17ISVAGAQSIRR118 pKa = 11.84QTLDD122 pKa = 2.6MWIDD126 pKa = 3.88SPGHH130 pKa = 5.29NQLLVNLNSYY140 pKa = 11.34SDD142 pKa = 3.94DD143 pKa = 2.94TWNAIGVGVGFNAPVSGGSFTYY165 pKa = 10.83GNYY168 pKa = 8.37FHH170 pKa = 6.87VWFGKK175 pKa = 10.07VADD178 pKa = 4.05PAGPPVIDD186 pKa = 3.55GTNAGEE192 pKa = 4.47LIEE195 pKa = 4.26MTNFADD201 pKa = 3.99EE202 pKa = 4.14VRR204 pKa = 11.84DD205 pKa = 3.55RR206 pKa = 11.84GGNDD210 pKa = 3.04TVIGYY215 pKa = 9.49SGNDD219 pKa = 3.07TFYY222 pKa = 11.37VGGGANDD229 pKa = 3.28FRR231 pKa = 11.84GGDD234 pKa = 3.49GTDD237 pKa = 2.87RR238 pKa = 11.84AVIPATFAQVTATVDD253 pKa = 3.29GSVAQITLNGTTSTYY268 pKa = 10.72RR269 pKa = 11.84GIEE272 pKa = 3.77VFAFSDD278 pKa = 3.54GTTRR282 pKa = 11.84ALGQLNGGTSGGDD295 pKa = 2.97RR296 pKa = 11.84DD297 pKa = 3.98GASDD301 pKa = 3.68GGGVGAPQDD310 pKa = 3.69QEE312 pKa = 4.82AVDD315 pKa = 4.64LFPVSSDD322 pKa = 2.91RR323 pKa = 11.84GEE325 pKa = 3.94TGEE328 pKa = 4.35AGGSGGEE335 pKa = 4.13GQEE338 pKa = 4.79MIGTDD343 pKa = 3.57AGDD346 pKa = 4.28EE347 pKa = 4.15ISGTSADD354 pKa = 4.13DD355 pKa = 4.02TIAGQGGHH363 pKa = 7.09DD364 pKa = 3.91MLSAGGGNDD373 pKa = 3.88NISGSDD379 pKa = 3.63GNDD382 pKa = 3.41TISAGSGHH390 pKa = 7.07DD391 pKa = 3.57NVGGGLGNDD400 pKa = 4.38EE401 pKa = 3.72IWGAGGNDD409 pKa = 3.68TIGAGRR415 pKa = 11.84GNDD418 pKa = 3.59YY419 pKa = 11.5VNAGPGNDD427 pKa = 3.54VANGGQGDD435 pKa = 4.06DD436 pKa = 4.22MIFGEE441 pKa = 5.53DD442 pKa = 3.82GDD444 pKa = 4.34DD445 pKa = 3.39TTGAGFGDD453 pKa = 4.05DD454 pKa = 3.67TVAGGSGHH462 pKa = 7.39DD463 pKa = 3.79SLGGGTGQDD472 pKa = 3.63MIAGHH477 pKa = 6.85GGNDD481 pKa = 3.81AIGGGEE487 pKa = 4.27GNDD490 pKa = 4.14SISGGDD496 pKa = 3.59GNDD499 pKa = 3.32FLAGGGRR506 pKa = 11.84DD507 pKa = 3.48DD508 pKa = 4.57VIYY511 pKa = 10.42GGSGADD517 pKa = 3.87TINGGDD523 pKa = 4.25GNDD526 pKa = 3.46ALYY529 pKa = 10.69GGTGADD535 pKa = 2.89VFVWNEE541 pKa = 3.85YY542 pKa = 9.8EE543 pKa = 4.92AGATDD548 pKa = 4.35TIYY551 pKa = 10.97GFQDD555 pKa = 3.26GVDD558 pKa = 3.8TLRR561 pKa = 11.84LADD564 pKa = 3.66VSNAPGTGLAGKK576 pKa = 10.09LDD578 pKa = 4.06ALNITDD584 pKa = 3.56IVVDD588 pKa = 4.55GVAGVRR594 pKa = 11.84VGYY597 pKa = 9.55QGNGIEE603 pKa = 4.69IPGLSAAQITLDD615 pKa = 4.46DD616 pKa = 3.76IMFII620 pKa = 4.45

Molecular weight: 62.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4HL11|W4HL11_9RHOB Uncharacterized protein OS=Roseivivax marinus OX=1379903 GN=ATO8_05176 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84AATHH6 pKa = 6.31IAWPAPRR13 pKa = 11.84RR14 pKa = 11.84SPWTPLRR21 pKa = 11.84ATAAALLLCLSLPVTGAGPASAQTIGAMSPHH52 pKa = 7.13PGAGVSDD59 pKa = 3.72QAVEE63 pKa = 3.9VSLRR67 pKa = 11.84PKK69 pKa = 10.44VRR71 pKa = 11.84DD72 pKa = 3.35DD73 pKa = 4.09RR74 pKa = 11.84LPKK77 pKa = 10.38ARR79 pKa = 11.84WGTSARR85 pKa = 11.84GQTWTRR91 pKa = 11.84AAVSALRR98 pKa = 11.84GPASGLTDD106 pKa = 4.02IVPGDD111 pKa = 3.53IDD113 pKa = 3.84TWCPAYY119 pKa = 10.21EE120 pKa = 4.24EE121 pKa = 4.2NNRR124 pKa = 11.84RR125 pKa = 11.84LRR127 pKa = 11.84EE128 pKa = 4.19AFWVSLVSALAKK140 pKa = 10.45HH141 pKa = 5.99EE142 pKa = 4.29STYY145 pKa = 10.92RR146 pKa = 11.84PTVVGGGGRR155 pKa = 11.84WHH157 pKa = 7.22GLLQILPSTARR168 pKa = 11.84LYY170 pKa = 10.95GCRR173 pKa = 11.84AGSGAALQSGAANLSCGLRR192 pKa = 11.84IMARR196 pKa = 11.84TVARR200 pKa = 11.84DD201 pKa = 3.67GVVSAGMRR209 pKa = 11.84GVAADD214 pKa = 3.82WGPFHH219 pKa = 7.42SSTKK223 pKa = 9.54RR224 pKa = 11.84SDD226 pKa = 3.29MIAYY230 pKa = 7.28TRR232 pKa = 11.84SQAFCKK238 pKa = 9.91SVPGKK243 pKa = 10.58RR244 pKa = 11.84PMARR248 pKa = 11.84PDD250 pKa = 3.14VDD252 pKa = 3.54EE253 pKa = 4.32TVPLYY258 pKa = 11.16AEE260 pKa = 4.56IPPRR264 pKa = 11.84DD265 pKa = 3.53APVFSTQGRR274 pKa = 11.84LAPP277 pKa = 4.36

Molecular weight: 29.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4264

0

4264

1352812

29

5131

317.3

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.077 ± 0.056

0.814 ± 0.014

6.556 ± 0.046

6.243 ± 0.037

3.52 ± 0.02

9.164 ± 0.058

1.949 ± 0.022

4.6 ± 0.028

2.375 ± 0.035

9.834 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.022

2.263 ± 0.023

5.22 ± 0.028

2.805 ± 0.023

7.317 ± 0.053

5.045 ± 0.029

5.688 ± 0.044

7.519 ± 0.03

1.391 ± 0.017

2.074 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski