Amino acid dipepetide frequency for Clostridium tetanomorphum DSM 665

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.856AlaAla: 3.856 ± 0.074	0.719AlaCys: 0.719 ± 0.027	2.299AlaAsp: 2.299 ± 0.048	3.328AlaGlu: 3.328 ± 0.057	2.497AlaPhe: 2.497 ± 0.04	3.492AlaGly: 3.492 ± 0.067	0.79AlaHis: 0.79 ± 0.025	5.781AlaIle: 5.781 ± 0.081	4.626AlaLys: 4.626 ± 0.074	5.779AlaLeu: 5.779 ± 0.083	1.578AlaMet: 1.578 ± 0.037	2.542AlaAsn: 2.542 ± 0.043	1.354AlaPro: 1.354 ± 0.034	1.323AlaGln: 1.323 ± 0.033	1.682AlaArg: 1.682 ± 0.041	3.278AlaSer: 3.278 ± 0.057	2.549AlaThr: 2.549 ± 0.055	3.915AlaVal: 3.915 ± 0.062	0.318AlaTrp: 0.318 ± 0.016	2.089AlaTyr: 2.089 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.566CysAla: 0.566 ± 0.021	0.202CysCys: 0.202 ± 0.013	0.639CysAsp: 0.639 ± 0.024	0.802CysGlu: 0.802 ± 0.025	0.549CysPhe: 0.549 ± 0.02	1.09CysGly: 1.09 ± 0.031	0.213CysHis: 0.213 ± 0.013	1.288CysIle: 1.288 ± 0.033	1.103CysLys: 1.103 ± 0.027	0.828CysLeu: 0.828 ± 0.028	0.295CysMet: 0.295 ± 0.017	0.82CysAsn: 0.82 ± 0.028	0.483CysPro: 0.483 ± 0.023	0.227CysGln: 0.227 ± 0.013	0.404CysArg: 0.404 ± 0.018	0.813CysSer: 0.813 ± 0.027	0.593CysThr: 0.593 ± 0.022	0.639CysVal: 0.639 ± 0.024	0.065CysTrp: 0.065 ± 0.006	0.501CysTyr: 0.501 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	2.409AspAla: 2.409 ± 0.055	0.574AspCys: 0.574 ± 0.021	2.318AspAsp: 2.318 ± 0.05	4.23AspGlu: 4.23 ± 0.062	2.642AspPhe: 2.642 ± 0.048	3.109AspGly: 3.109 ± 0.054	0.542AspHis: 0.542 ± 0.022	6.337AspIle: 6.337 ± 0.078	5.797AspLys: 5.797 ± 0.085	4.558AspLeu: 4.558 ± 0.069	1.517AspMet: 1.517 ± 0.034	3.373AspAsn: 3.373 ± 0.059	1.226AspPro: 1.226 ± 0.037	0.701AspGln: 0.701 ± 0.022	1.758AspArg: 1.758 ± 0.035	2.948AspSer: 2.948 ± 0.047	2.419AspThr: 2.419 ± 0.041	3.17AspVal: 3.17 ± 0.055	0.327AspTrp: 0.327 ± 0.017	2.455AspTyr: 2.455 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	3.904GluAla: 3.904 ± 0.065	0.697GluCys: 0.697 ± 0.025	4.328GluAsp: 4.328 ± 0.07	7.309GluGlu: 7.309 ± 0.111	2.897GluPhe: 2.897 ± 0.053	4.126GluGly: 4.126 ± 0.068	0.92GluHis: 0.92 ± 0.031	7.199GluIle: 7.199 ± 0.079	8.184GluLys: 8.184 ± 0.095	6.429GluLeu: 6.429 ± 0.089	1.918GluMet: 1.918 ± 0.042	5.638GluAsn: 5.638 ± 0.077	1.385GluPro: 1.385 ± 0.033	1.804GluGln: 1.804 ± 0.044	2.589GluArg: 2.589 ± 0.046	3.379GluSer: 3.379 ± 0.051	2.742GluThr: 2.742 ± 0.044	4.718GluVal: 4.718 ± 0.065	0.449GluTrp: 0.449 ± 0.018	2.964GluTyr: 2.964 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.155PheAla: 2.155 ± 0.044	0.526PheCys: 0.526 ± 0.02	2.228PheAsp: 2.228 ± 0.04	2.359PheGlu: 2.359 ± 0.044	2.051PhePhe: 2.051 ± 0.05	2.713PheGly: 2.713 ± 0.055	0.598PheHis: 0.598 ± 0.023	5.219PheIle: 5.219 ± 0.088	4.145PheLys: 4.145 ± 0.065	4.065PheLeu: 4.065 ± 0.071	1.199PheMet: 1.199 ± 0.03	3.148PheAsn: 3.148 ± 0.053	1.193PhePro: 1.193 ± 0.038	1.112PheGln: 1.112 ± 0.031	1.25PheArg: 1.25 ± 0.034	3.24PheSer: 3.24 ± 0.057	2.357PheThr: 2.357 ± 0.048	2.482PheVal: 2.482 ± 0.045	0.345PheTrp: 0.345 ± 0.017	1.868PheTyr: 1.868 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	3.842GlyAla: 3.842 ± 0.072	0.842GlyCys: 0.842 ± 0.031	3.124GlyAsp: 3.124 ± 0.049	4.551GlyGlu: 4.551 ± 0.071	3.001GlyPhe: 3.001 ± 0.051	4.077GlyGly: 4.077 ± 0.074	0.935GlyHis: 0.935 ± 0.032	6.962GlyIle: 6.962 ± 0.09	6.106GlyLys: 6.106 ± 0.067	5.159GlyLeu: 5.159 ± 0.081	1.711GlyMet: 1.711 ± 0.039	3.345GlyAsn: 3.345 ± 0.054	1.123GlyPro: 1.123 ± 0.035	1.422GlyGln: 1.422 ± 0.038	2.174GlyArg: 2.174 ± 0.041	3.46GlySer: 3.46 ± 0.051	3.317GlyThr: 3.317 ± 0.059	4.311GlyVal: 4.311 ± 0.074	0.509GlyTrp: 0.509 ± 0.023	2.833GlyTyr: 2.833 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	0.653HisAla: 0.653 ± 0.023	0.199HisCys: 0.199 ± 0.013	0.606HisAsp: 0.606 ± 0.022	0.81HisGlu: 0.81 ± 0.023	0.631HisPhe: 0.631 ± 0.021	0.956HisGly: 0.956 ± 0.03	0.261HisHis: 0.261 ± 0.015	1.501HisIle: 1.501 ± 0.035	1.097HisLys: 1.097 ± 0.032	1.143HisLeu: 1.143 ± 0.031	0.403HisMet: 0.403 ± 0.017	0.902HisAsn: 0.902 ± 0.025	0.617HisPro: 0.617 ± 0.022	0.322HisGln: 0.322 ± 0.016	0.495HisArg: 0.495 ± 0.023	0.87HisSer: 0.87 ± 0.025	0.656HisThr: 0.656 ± 0.023	0.692HisVal: 0.692 ± 0.024	0.121HisTrp: 0.121 ± 0.011	0.586HisTyr: 0.586 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.723IleAla: 5.723 ± 0.075	1.364IleCys: 1.364 ± 0.036	5.842IleAsp: 5.842 ± 0.08	7.303IleGlu: 7.303 ± 0.084	4.692IlePhe: 4.692 ± 0.087	6.391IleGly: 6.391 ± 0.097	1.373IleHis: 1.373 ± 0.03	10.649IleIle: 10.649 ± 0.128	9.871IleLys: 9.871 ± 0.096	9.76IleLeu: 9.76 ± 0.116	2.576IleMet: 2.576 ± 0.053	7.102IleAsn: 7.102 ± 0.085	3.534IlePro: 3.534 ± 0.05	2.333IleGln: 2.333 ± 0.039	3.156IleArg: 3.156 ± 0.059	7.336IleSer: 7.336 ± 0.091	5.129IleThr: 5.129 ± 0.064	6.448IleVal: 6.448 ± 0.072	0.586IleTrp: 0.586 ± 0.024	4.073IleTyr: 4.073 ± 0.061	0.0IleXaa: 0.0 ± 0.0
Lys	4.966LysAla: 4.966 ± 0.075	0.906LysCys: 0.906 ± 0.031	6.506LysAsp: 6.506 ± 0.098	9.541LysGlu: 9.541 ± 0.099	3.52LysPhe: 3.52 ± 0.053	5.625LysGly: 5.625 ± 0.056	1.2LysHis: 1.2 ± 0.031	8.999LysIle: 8.999 ± 0.093	8.877LysLys: 8.877 ± 0.099	7.982LysLeu: 7.982 ± 0.091	2.408LysMet: 2.408 ± 0.043	7.32LysAsn: 7.32 ± 0.094	2.132LysPro: 2.132 ± 0.042	2.205LysGln: 2.205 ± 0.045	3.134LysArg: 3.134 ± 0.044	5.326LysSer: 5.326 ± 0.072	3.974LysThr: 3.974 ± 0.056	6.161LysVal: 6.161 ± 0.078	0.683LysTrp: 0.683 ± 0.024	4.312LysTyr: 4.312 ± 0.061	0.0LysXaa: 0.0 ± 0.0
Leu	4.895LeuAla: 4.895 ± 0.068	1.17LeuCys: 1.17 ± 0.033	4.709LeuAsp: 4.709 ± 0.059	5.965LeuGlu: 5.965 ± 0.085	3.767LeuPhe: 3.767 ± 0.072	5.91LeuGly: 5.91 ± 0.077	1.129LeuHis: 1.129 ± 0.03	8.566LeuIle: 8.566 ± 0.107	9.009LeuLys: 9.009 ± 0.087	7.795LeuLeu: 7.795 ± 0.09	2.375LeuMet: 2.375 ± 0.041	6.349LeuAsn: 6.349 ± 0.083	2.849LeuPro: 2.849 ± 0.052	2.352LeuGln: 2.352 ± 0.044	2.971LeuArg: 2.971 ± 0.053	6.459LeuSer: 6.459 ± 0.078	4.412LeuThr: 4.412 ± 0.06	5.123LeuVal: 5.123 ± 0.07	0.601LeuTrp: 0.601 ± 0.022	3.334LeuTyr: 3.334 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.711MetAla: 1.711 ± 0.038	0.306MetCys: 0.306 ± 0.016	1.624MetAsp: 1.624 ± 0.037	2.005MetGlu: 2.005 ± 0.044	1.02MetPhe: 1.02 ± 0.031	1.784MetGly: 1.784 ± 0.044	0.325MetHis: 0.325 ± 0.016	2.345MetIle: 2.345 ± 0.044	2.652MetLys: 2.652 ± 0.044	2.254MetLeu: 2.254 ± 0.044	0.669MetMet: 0.669 ± 0.026	1.821MetAsn: 1.821 ± 0.039	0.918MetPro: 0.918 ± 0.028	0.642MetGln: 0.642 ± 0.022	0.86MetArg: 0.86 ± 0.025	1.623MetSer: 1.623 ± 0.037	1.166MetThr: 1.166 ± 0.032	1.571MetVal: 1.571 ± 0.035	0.159MetTrp: 0.159 ± 0.012	0.933MetTyr: 0.933 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.762AsnAla: 2.762 ± 0.049	0.783AsnCys: 0.783 ± 0.028	2.859AsnAsp: 2.859 ± 0.053	4.472AsnGlu: 4.472 ± 0.069	2.844AsnPhe: 2.844 ± 0.047	3.804AsnGly: 3.804 ± 0.061	0.817AsnHis: 0.817 ± 0.025	8.343AsnIle: 8.343 ± 0.104	6.985AsnLys: 6.985 ± 0.088	6.044AsnLeu: 6.044 ± 0.08	1.761AsnMet: 1.761 ± 0.037	4.993AsnAsn: 4.993 ± 0.077	2.053AsnPro: 2.053 ± 0.04	1.154AsnGln: 1.154 ± 0.03	2.076AsnArg: 2.076 ± 0.042	4.277AsnSer: 4.277 ± 0.066	3.063AsnThr: 3.063 ± 0.044	3.763AsnVal: 3.763 ± 0.052	0.489AsnTrp: 0.489 ± 0.021	2.916AsnTyr: 2.916 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	1.387ProAla: 1.387 ± 0.033	0.344ProCys: 0.344 ± 0.017	1.361ProAsp: 1.361 ± 0.033	2.142ProGlu: 2.142 ± 0.045	1.388ProPhe: 1.388 ± 0.037	1.651ProGly: 1.651 ± 0.042	0.462ProHis: 0.462 ± 0.018	3.016ProIle: 3.016 ± 0.051	2.309ProLys: 2.309 ± 0.043	2.538ProLeu: 2.538 ± 0.048	0.746ProMet: 0.746 ± 0.025	1.577ProAsn: 1.577 ± 0.037	0.666ProPro: 0.666 ± 0.026	0.78ProGln: 0.78 ± 0.024	0.776ProArg: 0.776 ± 0.028	1.593ProSer: 1.593 ± 0.039	1.434ProThr: 1.434 ± 0.036	1.958ProVal: 1.958 ± 0.042	0.253ProTrp: 0.253 ± 0.016	1.349ProTyr: 1.349 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.359GlnAla: 1.359 ± 0.041	0.349GlnCys: 0.349 ± 0.018	1.1GlnAsp: 1.1 ± 0.029	1.568GlnGlu: 1.568 ± 0.039	0.945GlnPhe: 0.945 ± 0.026	1.501GlnGly: 1.501 ± 0.037	0.35GlnHis: 0.35 ± 0.016	2.308GlnIle: 2.308 ± 0.039	1.995GlnLys: 1.995 ± 0.038	2.135GlnLeu: 2.135 ± 0.045	0.663GlnMet: 0.663 ± 0.025	1.427GlnAsn: 1.427 ± 0.037	0.595GlnPro: 0.595 ± 0.022	0.763GlnGln: 0.763 ± 0.025	0.897GlnArg: 0.897 ± 0.03	1.394GlnSer: 1.394 ± 0.033	0.99GlnThr: 0.99 ± 0.03	1.433GlnVal: 1.433 ± 0.032	0.258GlnTrp: 0.258 ± 0.014	1.088GlnTyr: 1.088 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	1.738ArgAla: 1.738 ± 0.037	0.392ArgCys: 0.392 ± 0.017	1.719ArgAsp: 1.719 ± 0.035	2.823ArgGlu: 2.823 ± 0.056	1.394ArgPhe: 1.394 ± 0.032	1.923ArgGly: 1.923 ± 0.033	0.48ArgHis: 0.48 ± 0.018	3.28ArgIle: 3.28 ± 0.052	3.156ArgLys: 3.156 ± 0.06	2.846ArgLeu: 2.846 ± 0.05	0.88ArgMet: 0.88 ± 0.027	2.076ArgAsn: 2.076 ± 0.038	0.806ArgPro: 0.806 ± 0.028	0.946ArgGln: 0.946 ± 0.028	1.45ArgArg: 1.45 ± 0.04	1.464ArgSer: 1.464 ± 0.038	1.459ArgThr: 1.459 ± 0.034	2.12ArgVal: 2.12 ± 0.043	0.255ArgTrp: 0.255 ± 0.015	1.447ArgTyr: 1.447 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.051SerAla: 3.051 ± 0.054	0.693SerCys: 0.693 ± 0.023	2.718SerAsp: 2.718 ± 0.049	3.821SerGlu: 3.821 ± 0.056	3.142SerPhe: 3.142 ± 0.054	4.09SerGly: 4.09 ± 0.071	0.905SerHis: 0.905 ± 0.025	7.182SerIle: 7.182 ± 0.088	5.953SerLys: 5.953 ± 0.081	6.039SerLeu: 6.039 ± 0.088	1.737SerMet: 1.737 ± 0.04	3.899SerAsn: 3.899 ± 0.061	1.682SerPro: 1.682 ± 0.035	1.545SerGln: 1.545 ± 0.039	1.939SerArg: 1.939 ± 0.042	4.298SerSer: 4.298 ± 0.072	2.983SerThr: 2.983 ± 0.054	3.579SerVal: 3.579 ± 0.058	0.409SerTrp: 0.409 ± 0.017	2.593SerTyr: 2.593 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	3.006ThrAla: 3.006 ± 0.056	0.506ThrCys: 0.506 ± 0.02	2.225ThrAsp: 2.225 ± 0.042	2.859ThrGlu: 2.859 ± 0.048	2.161ThrPhe: 2.161 ± 0.04	3.441ThrGly: 3.441 ± 0.059	0.73ThrHis: 0.73 ± 0.025	5.014ThrIle: 5.014 ± 0.064	3.697ThrLys: 3.697 ± 0.057	4.654ThrLeu: 4.654 ± 0.065	1.161ThrMet: 1.161 ± 0.029	2.686ThrAsn: 2.686 ± 0.053	1.768ThrPro: 1.768 ± 0.035	1.026ThrGln: 1.026 ± 0.03	1.374ThrArg: 1.374 ± 0.032	3.042ThrSer: 3.042 ± 0.05	2.539ThrThr: 2.539 ± 0.054	3.343ThrVal: 3.343 ± 0.056	0.333ThrTrp: 0.333 ± 0.018	1.806ThrTyr: 1.806 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	3.595ValAla: 3.595 ± 0.069	0.816ValCys: 0.816 ± 0.026	3.513ValAsp: 3.513 ± 0.054	4.294ValGlu: 4.294 ± 0.059	2.788ValPhe: 2.788 ± 0.048	4.064ValGly: 4.064 ± 0.067	0.823ValHis: 0.823 ± 0.026	6.051ValIle: 6.051 ± 0.085	5.537ValLys: 5.537 ± 0.076	5.641ValLeu: 5.641 ± 0.071	1.563ValMet: 1.563 ± 0.04	3.765ValAsn: 3.765 ± 0.054	1.929ValPro: 1.929 ± 0.038	1.505ValGln: 1.505 ± 0.032	1.895ValArg: 1.895 ± 0.043	4.221ValSer: 4.221 ± 0.063	3.25ValThr: 3.25 ± 0.056	4.261ValVal: 4.261 ± 0.061	0.417ValTrp: 0.417 ± 0.019	2.379ValTyr: 2.379 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	0.356TrpAla: 0.356 ± 0.019	0.111TrpCys: 0.111 ± 0.009	0.416TrpAsp: 0.416 ± 0.02	0.439TrpGlu: 0.439 ± 0.021	0.309TrpPhe: 0.309 ± 0.016	0.479TrpGly: 0.479 ± 0.022	0.129TrpHis: 0.129 ± 0.012	0.743TrpIle: 0.743 ± 0.028	0.617TrpLys: 0.617 ± 0.021	0.569TrpLeu: 0.569 ± 0.019	0.196TrpMet: 0.196 ± 0.013	0.52TrpAsn: 0.52 ± 0.021	0.172TrpPro: 0.172 ± 0.011	0.218TrpGln: 0.218 ± 0.013	0.258TrpArg: 0.258 ± 0.015	0.422TrpSer: 0.422 ± 0.02	0.328TrpThr: 0.328 ± 0.017	0.356TrpVal: 0.356 ± 0.019	0.095TrpTrp: 0.095 ± 0.008	0.241TrpTyr: 0.241 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.862TyrAla: 1.862 ± 0.034	0.62TyrCys: 0.62 ± 0.022	2.273TyrAsp: 2.273 ± 0.05	2.959TyrGlu: 2.959 ± 0.049	2.015TyrPhe: 2.015 ± 0.043	2.577TyrGly: 2.577 ± 0.049	0.539TyrHis: 0.539 ± 0.019	4.456TyrIle: 4.456 ± 0.069	4.064TyrLys: 4.064 ± 0.059	3.524TyrLeu: 3.524 ± 0.06	1.03TyrMet: 1.03 ± 0.031	2.954TyrAsn: 2.954 ± 0.059	1.246TyrPro: 1.246 ± 0.035	0.735TyrGln: 0.735 ± 0.024	1.514TyrArg: 1.514 ± 0.034	2.817TyrSer: 2.817 ± 0.047	2.009TyrThr: 2.009 ± 0.044	2.266TyrVal: 2.266 ± 0.044	0.306TyrTrp: 0.306 ± 0.018	1.932TyrTyr: 1.932 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4354 proteins (1299553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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