Witwatersrand virus
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R7FKB0|A0A0R7FKB0_9VIRU Non-structural protein NS-S OS=Witwatersrand virus OX=1678231 PE=4 SV=1
MM1 pKa = 7.28 QNHH4 pKa = 5.55 LQEE7 pKa = 4.35 LARR10 pKa = 11.84 LRR12 pKa = 11.84 GRR14 pKa = 11.84 LRR16 pKa = 11.84 GAQDD20 pKa = 3.37 PADD23 pKa = 3.82 GKK25 pKa = 10.46 EE26 pKa = 4.44 LYY28 pKa = 9.06 TAALMLRR35 pKa = 11.84 HH36 pKa = 7.02 DD37 pKa = 3.77 YY38 pKa = 10.75 FGYY41 pKa = 9.79 QLCQAYY47 pKa = 9.62 GIPYY51 pKa = 9.94 RR52 pKa = 11.84 NDD54 pKa = 2.94 VPLEE58 pKa = 4.7 DD59 pKa = 4.2 ILLEE63 pKa = 4.18 TTEE66 pKa = 3.96 VDD68 pKa = 3.07 AANIKK73 pKa = 10.43 IPDD76 pKa = 3.53 ITPDD80 pKa = 2.65 NFMFHH85 pKa = 7.4 RR86 pKa = 11.84 GRR88 pKa = 11.84 LVIIDD93 pKa = 3.88 YY94 pKa = 9.93 KK95 pKa = 11.13 VSVSTEE101 pKa = 3.87 STDD104 pKa = 3.68 YY105 pKa = 10.52 TLKK108 pKa = 10.58 KK109 pKa = 10.25 YY110 pKa = 10.96 NEE112 pKa = 4.94 AIEE115 pKa = 4.22 KK116 pKa = 8.83 VQPFVNLRR124 pKa = 11.84 IGLCIIRR131 pKa = 11.84 MHH133 pKa = 7.38 PITNQIMFTDD143 pKa = 4.39 DD144 pKa = 4.22 EE145 pKa = 4.32 IAHH148 pKa = 6.77 DD149 pKa = 4.51 FGHH152 pKa = 6.06 IHH154 pKa = 6.72 IPLDD158 pKa = 3.25 FTPFVEE164 pKa = 5.82 FISDD168 pKa = 3.67 VKK170 pKa = 11.1 EE171 pKa = 3.95 KK172 pKa = 10.72 FHH174 pKa = 7.85 DD175 pKa = 3.82 SEE177 pKa = 4.98 EE178 pKa = 4.08 FMEE181 pKa = 5.1 MIAHH185 pKa = 6.75 GDD187 pKa = 3.27 VTFTVRR193 pKa = 11.84 WCNEE197 pKa = 3.96 EE198 pKa = 4.38 CPEE201 pKa = 5.21 LYY203 pKa = 10.35 NHH205 pKa = 7.18 PNFTEE210 pKa = 4.65 FMSTMSDD217 pKa = 3.13 NYY219 pKa = 10.63 QEE221 pKa = 4.52 MFLEE225 pKa = 4.78 SINTCAYY232 pKa = 9.53 KK233 pKa = 10.69 AEE235 pKa = 4.08 RR236 pKa = 11.84 WDD238 pKa = 3.56 TNLRR242 pKa = 11.84 KK243 pKa = 9.96 VRR245 pKa = 11.84 DD246 pKa = 3.64 QTRR249 pKa = 11.84 QEE251 pKa = 4.16 YY252 pKa = 10.79 EE253 pKa = 3.72 EE254 pKa = 4.2 FVYY257 pKa = 10.72 KK258 pKa = 10.43 EE259 pKa = 4.11 SKK261 pKa = 9.81 KK262 pKa = 11.07 LLMCDD267 pKa = 3.45 GNYY270 pKa = 9.86 KK271 pKa = 10.4 EE272 pKa = 4.8 PSQDD276 pKa = 3.78 EE277 pKa = 4.18 IDD279 pKa = 4.44 LGWSEE284 pKa = 3.83 MTEE287 pKa = 4.32 RR288 pKa = 11.84 IMSMRR293 pKa = 11.84 EE294 pKa = 3.61 VSQDD298 pKa = 3.64 LSDD301 pKa = 3.72 QKK303 pKa = 11.13 PSIHH307 pKa = 6.83 FIWAPHH313 pKa = 6.34 DD314 pKa = 3.88 KK315 pKa = 10.23 EE316 pKa = 5.37 LPTHH320 pKa = 5.7 TTQKK324 pKa = 10.96 LKK326 pKa = 10.8 FLAKK330 pKa = 9.79 ILQAIEE336 pKa = 3.78 DD337 pKa = 3.61 RR338 pKa = 11.84 TRR340 pKa = 11.84 YY341 pKa = 9.78 SEE343 pKa = 3.86 EE344 pKa = 3.99 FKK346 pKa = 11.04 RR347 pKa = 11.84 LGYY350 pKa = 11.24 LMDD353 pKa = 4.63 IDD355 pKa = 5.4 GNDD358 pKa = 3.14 HH359 pKa = 6.68 VYY361 pKa = 9.18 EE362 pKa = 4.4 TFCADD367 pKa = 3.95 LKK369 pKa = 11.26 AKK371 pKa = 10.24 ARR373 pKa = 11.84 SKK375 pKa = 10.65 SGQVKK380 pKa = 7.65 NQRR383 pKa = 11.84 MEE385 pKa = 3.9 PLKK388 pKa = 10.72 IGNAYY393 pKa = 9.15 VLWEE397 pKa = 3.99 QQFLLPASEE406 pKa = 4.16 MGKK409 pKa = 8.36 HH410 pKa = 5.13 QRR412 pKa = 11.84 SHH414 pKa = 6.31 LLKK417 pKa = 10.79 DD418 pKa = 3.49 FFGIGKK424 pKa = 9.19 HH425 pKa = 5.89 KK426 pKa = 10.66 NFSKK430 pKa = 10.62 KK431 pKa = 10.38 GIEE434 pKa = 5.22 DD435 pKa = 3.43 IDD437 pKa = 3.48 ISKK440 pKa = 10.67 PKK442 pKa = 10.4 ILDD445 pKa = 3.81 FNDD448 pKa = 3.3 KK449 pKa = 10.47 NIKK452 pKa = 9.35 EE453 pKa = 4.44 SAYY456 pKa = 10.18 TMMDD460 pKa = 3.14 RR461 pKa = 11.84 TKK463 pKa = 10.83 LALSEE468 pKa = 4.24 RR469 pKa = 11.84 NGLEE473 pKa = 3.57 PHH475 pKa = 6.78 HH476 pKa = 7.21 PITDD480 pKa = 3.54 EE481 pKa = 4.01 YY482 pKa = 10.3 MSQIDD487 pKa = 3.97 ASNHH491 pKa = 3.84 LTALTIQKK499 pKa = 9.35 VFKK502 pKa = 9.4 TKK504 pKa = 10.09 YY505 pKa = 7.46 WSCVTDD511 pKa = 3.3 ISVLMRR517 pKa = 11.84 NILSISQYY525 pKa = 10.56 NRR527 pKa = 11.84 ANTFRR532 pKa = 11.84 VAMCANNSLYY542 pKa = 10.97 ALVMPSTDD550 pKa = 2.81 IKK552 pKa = 9.79 TKK554 pKa = 9.49 EE555 pKa = 3.9 ATVVYY560 pKa = 9.41 CIIAYY565 pKa = 9.68 HH566 pKa = 6.52 KK567 pKa = 10.91 EE568 pKa = 3.87 EE569 pKa = 3.97 TSVFNPGALHH579 pKa = 4.49 YY580 pKa = 9.16 TYY582 pKa = 10.36 RR583 pKa = 11.84 CPGGFLSISRR593 pKa = 11.84 AMRR596 pKa = 11.84 LDD598 pKa = 3.15 KK599 pKa = 10.73 KK600 pKa = 9.53 RR601 pKa = 11.84 CQRR604 pKa = 11.84 IVTAPGIFLTTTCSFKK620 pKa = 10.95 SDD622 pKa = 3.84 NEE624 pKa = 4.43 TIDD627 pKa = 3.74 INDD630 pKa = 3.36 IMNFALFTSLNVTKK644 pKa = 10.92 SMLSLTEE651 pKa = 3.7 PSRR654 pKa = 11.84 YY655 pKa = 9.4 MIMNSLAVSSHH666 pKa = 3.34 VRR668 pKa = 11.84 EE669 pKa = 4.3 YY670 pKa = 10.54 ISEE673 pKa = 4.16 KK674 pKa = 9.97 FSPYY678 pKa = 9.81 TKK680 pKa = 9.97 TLFSVYY686 pKa = 9.39 MVDD689 pKa = 4.46 KK690 pKa = 10.57 IRR692 pKa = 11.84 RR693 pKa = 11.84 GCSRR697 pKa = 11.84 ANAQRR702 pKa = 11.84 EE703 pKa = 4.54 LIRR706 pKa = 11.84 LRR708 pKa = 11.84 NVHH711 pKa = 6.25 LTDD714 pKa = 4.7 FDD716 pKa = 3.69 ITQKK720 pKa = 10.73 GAQDD724 pKa = 3.63 NRR726 pKa = 11.84 DD727 pKa = 3.62 LDD729 pKa = 5.04 SIWFPGKK736 pKa = 8.93 VTLKK740 pKa = 10.42 EE741 pKa = 3.97 YY742 pKa = 10.97 LEE744 pKa = 4.16 QIYY747 pKa = 8.05 TVFYY751 pKa = 10.41 LNPKK755 pKa = 8.71 GLHH758 pKa = 5.74 EE759 pKa = 4.28 KK760 pKa = 9.44 HH761 pKa = 6.2 HH762 pKa = 6.39 VMVDD766 pKa = 3.32 LLKK769 pKa = 9.87 TVIEE773 pKa = 4.11 IEE775 pKa = 3.36 IDD777 pKa = 3.22 QRR779 pKa = 11.84 LNTVVPWSATPKK791 pKa = 9.06 KK792 pKa = 10.46 QSVNLPILINSIATNYY808 pKa = 8.23 MHH810 pKa = 7.04 DD811 pKa = 3.19 TSYY814 pKa = 11.11 CHH816 pKa = 6.93 HH817 pKa = 6.98 LRR819 pKa = 11.84 TRR821 pKa = 11.84 IEE823 pKa = 3.67 NRR825 pKa = 11.84 NNLRR829 pKa = 11.84 RR830 pKa = 11.84 SFSTISTFTSSKK842 pKa = 10.71 SCIKK846 pKa = 9.79 TGNFYY851 pKa = 10.43 QYY853 pKa = 11.54 KK854 pKa = 10.07 LNQSKK859 pKa = 9.78 KK860 pKa = 7.17 QKK862 pKa = 10.38 KK863 pKa = 9.6 AIQSEE868 pKa = 4.5 MKK870 pKa = 10.0 RR871 pKa = 11.84 SRR873 pKa = 11.84 IADD876 pKa = 3.59 TEE878 pKa = 4.36 HH879 pKa = 6.83 VHH881 pKa = 6.79 EE882 pKa = 4.72 EE883 pKa = 3.95 DD884 pKa = 3.56 RR885 pKa = 11.84 NLEE888 pKa = 4.22 VVHH891 pKa = 6.86 ANYY894 pKa = 10.52 EE895 pKa = 4.19 LTRR898 pKa = 11.84 QAIPDD903 pKa = 4.24 YY904 pKa = 10.24 IDD906 pKa = 4.76 CISTKK911 pKa = 10.26 VFDD914 pKa = 5.1 RR915 pKa = 11.84 LYY917 pKa = 11.01 EE918 pKa = 3.99 LYY920 pKa = 9.93 KK921 pKa = 11.07 YY922 pKa = 10.8 NIIDD926 pKa = 5.13 DD927 pKa = 4.72 GPCIQQIFKK936 pKa = 9.98 TMKK939 pKa = 8.69 EE940 pKa = 3.84 HH941 pKa = 6.12 NKK943 pKa = 10.05 FYY945 pKa = 10.07 FTFFNKK951 pKa = 9.4 GQKK954 pKa = 6.3 TAKK957 pKa = 10.03 DD958 pKa = 3.32 RR959 pKa = 11.84 EE960 pKa = 4.48 IFVGEE965 pKa = 4.43 YY966 pKa = 7.34 EE967 pKa = 5.31 AKK969 pKa = 8.32 MCMYY973 pKa = 10.31 LVEE976 pKa = 5.5 RR977 pKa = 11.84 IFKK980 pKa = 9.89 EE981 pKa = 4.02 RR982 pKa = 11.84 SRR984 pKa = 11.84 VNPDD988 pKa = 2.83 EE989 pKa = 4.9 MISEE993 pKa = 4.54 PGDD996 pKa = 3.37 GKK998 pKa = 10.8 LRR1000 pKa = 11.84 KK1001 pKa = 9.37 LEE1003 pKa = 4.11 KK1004 pKa = 10.29 NAEE1007 pKa = 3.89 KK1008 pKa = 10.41 EE1009 pKa = 3.68 IRR1011 pKa = 11.84 FLVEE1015 pKa = 3.89 KK1016 pKa = 10.69 LKK1018 pKa = 11.15 LKK1020 pKa = 10.76 NDD1022 pKa = 3.87 VIDD1025 pKa = 4.21 QEE1027 pKa = 4.02 IEE1029 pKa = 3.79 AAKK1032 pKa = 9.75 EE1033 pKa = 3.81 HH1034 pKa = 6.0 YY1035 pKa = 10.03 VYY1037 pKa = 11.03 KK1038 pKa = 10.37 LDD1040 pKa = 5.16 KK1041 pKa = 10.89 IEE1043 pKa = 4.32 ILQSQKK1049 pKa = 10.51 YY1050 pKa = 9.05 QGLKK1054 pKa = 10.74 LEE1056 pKa = 4.8 INADD1060 pKa = 3.47 MSKK1063 pKa = 10.21 WSAQDD1068 pKa = 3.14 VFYY1071 pKa = 10.94 KK1072 pKa = 9.86 YY1073 pKa = 10.28 FWAIALDD1080 pKa = 4.29 PILYY1084 pKa = 9.98 KK1085 pKa = 10.26 EE1086 pKa = 4.28 EE1087 pKa = 4.41 KK1088 pKa = 10.01 EE1089 pKa = 4.17 HH1090 pKa = 7.35 ILLFFCKK1097 pKa = 10.49 YY1098 pKa = 10.07 LDD1100 pKa = 3.88 KK1101 pKa = 11.12 EE1102 pKa = 4.47 LIIPDD1107 pKa = 3.55 EE1108 pKa = 4.06 VLYY1111 pKa = 11.23 NLLDD1115 pKa = 3.62 QQRR1118 pKa = 11.84 TYY1120 pKa = 11.07 PNDD1123 pKa = 3.38 IIKK1126 pKa = 10.35 QVTNNLTSNHH1136 pKa = 5.85 FNVKK1140 pKa = 10.25 KK1141 pKa = 10.53 NWLQGNFNYY1150 pKa = 9.53 MSSYY1154 pKa = 9.95 IHH1156 pKa = 6.12 SCAMSVYY1163 pKa = 10.57 KK1164 pKa = 10.46 DD1165 pKa = 3.56 IIKK1168 pKa = 9.11 ATAKK1172 pKa = 10.52 LIEE1175 pKa = 4.58 GEE1177 pKa = 4.38 CLVNTLVHH1185 pKa = 6.72 SDD1187 pKa = 4.18 DD1188 pKa = 3.93 NQTSITIINNRR1199 pKa = 11.84 IGHH1202 pKa = 5.68 KK1203 pKa = 10.13 QLTQHH1208 pKa = 7.22 CINEE1212 pKa = 4.23 FEE1214 pKa = 4.79 TICLTFGCQANMKK1227 pKa = 8.41 KK1228 pKa = 8.9 TYY1230 pKa = 8.08 ITNHH1234 pKa = 5.19 IKK1236 pKa = 10.74 EE1237 pKa = 4.38 FVSLFNIYY1245 pKa = 10.55 GEE1247 pKa = 4.21 PFSIYY1252 pKa = 10.56 NRR1254 pKa = 11.84 FILTSISDD1262 pKa = 3.72 CAYY1265 pKa = 9.08 IGPYY1269 pKa = 9.66 EE1270 pKa = 4.41 DD1271 pKa = 3.25 LASRR1275 pKa = 11.84 ISSAQTAIKK1284 pKa = 9.75 HH1285 pKa = 5.53 GCPSSLAWLSISIAHH1300 pKa = 7.22 WISYY1304 pKa = 7.59 LTYY1307 pKa = 11.27 NMLPGQINDD1316 pKa = 4.44 PLNVLPFEE1324 pKa = 4.41 SRR1326 pKa = 11.84 RR1327 pKa = 11.84 DD1328 pKa = 3.61 LPIEE1332 pKa = 3.92 LGGYY1336 pKa = 10.01 LDD1338 pKa = 5.27 APLSLIALAGLEE1350 pKa = 4.31 SGNLWFLIKK1359 pKa = 10.41 FIQKK1363 pKa = 10.08 HH1364 pKa = 4.69 GDD1366 pKa = 2.79 IMYY1369 pKa = 8.74 MKK1371 pKa = 9.91 EE1372 pKa = 4.16 AIVDD1376 pKa = 3.48 QVKK1379 pKa = 10.65 NIKK1382 pKa = 9.47 NWEE1385 pKa = 3.98 QLKK1388 pKa = 10.99 LSVQEE1393 pKa = 3.98 KK1394 pKa = 10.42 FKK1396 pKa = 11.4 LKK1398 pKa = 9.61 VLRR1401 pKa = 11.84 YY1402 pKa = 9.01 LVLDD1406 pKa = 3.9 AEE1408 pKa = 4.72 MDD1410 pKa = 3.5 NSDD1413 pKa = 3.76 VMGEE1417 pKa = 4.03 TSEE1420 pKa = 3.91 MRR1422 pKa = 11.84 GRR1424 pKa = 11.84 SLLTPRR1430 pKa = 11.84 KK1431 pKa = 7.98 FTTAGSLRR1439 pKa = 11.84 KK1440 pKa = 9.34 LVSFLDD1446 pKa = 3.81 FQEE1449 pKa = 4.36 AQRR1452 pKa = 11.84 QNNGLDD1458 pKa = 3.63 NIVQYY1463 pKa = 11.42 LLDD1466 pKa = 4.64 HH1467 pKa = 6.65 PTLLVTKK1474 pKa = 10.06 GEE1476 pKa = 4.28 SKK1478 pKa = 10.86 EE1479 pKa = 4.76 DD1480 pKa = 3.48 FMNSVLYY1487 pKa = 10.56 RR1488 pKa = 11.84 FNSKK1492 pKa = 9.76 KK1493 pKa = 10.45 FKK1495 pKa = 10.51 EE1496 pKa = 4.23 SLSIQNSSQLFLEE1509 pKa = 4.51 QILYY1513 pKa = 7.94 SHH1515 pKa = 6.86 KK1516 pKa = 10.69 PIVDD1520 pKa = 3.5 YY1521 pKa = 11.28 QGLKK1525 pKa = 10.89 DD1526 pKa = 4.27 KK1527 pKa = 11.15 FSVLNDD1533 pKa = 3.4 GTFLEE1538 pKa = 5.27 DD1539 pKa = 3.26 NPTIQGRR1546 pKa = 11.84 FTFPEE1551 pKa = 4.47 CFNKK1555 pKa = 9.71 IDD1557 pKa = 3.62 NDD1559 pKa = 3.69 LRR1561 pKa = 11.84 SLKK1564 pKa = 9.89 IDD1566 pKa = 3.92 LDD1568 pKa = 4.73 DD1569 pKa = 5.58 IEE1571 pKa = 5.17 VIYY1574 pKa = 11.09 NFIILNDD1581 pKa = 4.25 PLMVTAVNAHH1591 pKa = 6.74 ILQTTGIEE1599 pKa = 4.14 QPRR1602 pKa = 11.84 LGSTCSSMPEE1612 pKa = 3.78 FRR1614 pKa = 11.84 NLRR1617 pKa = 11.84 MIHH1620 pKa = 5.74 YY1621 pKa = 9.48 SPAVVLRR1628 pKa = 11.84 AYY1630 pKa = 10.5 ADD1632 pKa = 3.48 QNFEE1636 pKa = 5.46 LPGCDD1641 pKa = 3.62 PDD1643 pKa = 4.12 DD1644 pKa = 4.03 LRR1646 pKa = 11.84 RR1647 pKa = 11.84 DD1648 pKa = 3.58 VYY1650 pKa = 10.97 HH1651 pKa = 6.82 LEE1653 pKa = 4.09 KK1654 pKa = 10.57 FIEE1657 pKa = 4.38 TTGLKK1662 pKa = 9.76 IKK1664 pKa = 9.95 MEE1666 pKa = 4.03 NRR1668 pKa = 11.84 IKK1670 pKa = 10.94 ANEE1673 pKa = 4.04 GNKK1676 pKa = 10.49 GKK1678 pKa = 10.37 DD1679 pKa = 2.62 IFFEE1683 pKa = 4.3 IKK1685 pKa = 9.81 EE1686 pKa = 4.08 LTRR1689 pKa = 11.84 FYY1691 pKa = 10.19 QICYY1695 pKa = 9.16 EE1696 pKa = 4.18 YY1697 pKa = 11.24 VKK1699 pKa = 10.18 STEE1702 pKa = 4.3 HH1703 pKa = 5.95 KK1704 pKa = 8.38 VKK1706 pKa = 10.84 VYY1708 pKa = 10.34 ILPTKK1713 pKa = 10.54 SYY1715 pKa = 8.92 TQTDD1719 pKa = 4.0 FCAILQGSLMSDD1731 pKa = 3.56 KK1732 pKa = 10.96 NWVLVSHH1739 pKa = 6.81 LRR1741 pKa = 11.84 PVTAGGHH1748 pKa = 5.62 KK1749 pKa = 10.53 GSVQKK1754 pKa = 10.82 VVSHH1758 pKa = 6.61 DD1759 pKa = 3.37 AKK1761 pKa = 10.7 LAQEE1765 pKa = 4.42 AFLTLAHH1772 pKa = 6.17 FTDD1775 pKa = 3.99 TFLDD1779 pKa = 3.59 QQSRR1783 pKa = 11.84 IQFFNFIVEE1792 pKa = 4.11 NYY1794 pKa = 9.35 RR1795 pKa = 11.84 YY1796 pKa = 9.75 KK1797 pKa = 9.95 EE1798 pKa = 3.77 RR1799 pKa = 11.84 KK1800 pKa = 7.13 MQEE1803 pKa = 3.89 LLEE1806 pKa = 4.62 VILAGSDD1813 pKa = 3.04 RR1814 pKa = 11.84 VNYY1817 pKa = 10.4 LPILYY1822 pKa = 7.68 RR1823 pKa = 11.84 TKK1825 pKa = 10.47 QIQQQDD1831 pKa = 3.62 LDD1833 pKa = 4.56 LYY1835 pKa = 10.39 DD1836 pKa = 3.51 ARR1838 pKa = 11.84 KK1839 pKa = 10.2 SITRR1843 pKa = 11.84 VTWNEE1848 pKa = 3.15 WQMNRR1853 pKa = 11.84 TLDD1856 pKa = 3.45 TGPINIKK1863 pKa = 10.04 IEE1865 pKa = 4.34 TYY1867 pKa = 11.0 NSILTIIGMDD1877 pKa = 3.43 KK1878 pKa = 10.43 QLTMAQLEE1886 pKa = 4.62 VPEE1889 pKa = 4.03 ITYY1892 pKa = 10.23 TNIVHH1897 pKa = 6.59 NGYY1900 pKa = 9.85 RR1901 pKa = 11.84 LLNAKK1906 pKa = 9.95 HH1907 pKa = 5.69 GLAFEE1912 pKa = 4.6 RR1913 pKa = 11.84 MTEE1916 pKa = 4.19 INPEE1920 pKa = 3.95 PNNYY1924 pKa = 10.14 YY1925 pKa = 8.69 ITAQLKK1931 pKa = 8.87 SRR1933 pKa = 11.84 NRR1935 pKa = 11.84 YY1936 pKa = 7.33 VYY1938 pKa = 10.13 KK1939 pKa = 10.21 VYY1941 pKa = 10.28 EE1942 pKa = 4.21 YY1943 pKa = 11.28 KK1944 pKa = 10.87 SLIKK1948 pKa = 10.43 EE1949 pKa = 4.38 NNDD1952 pKa = 2.95 STRR1955 pKa = 11.84 LGLRR1959 pKa = 11.84 HH1960 pKa = 6.13 NPIKK1964 pKa = 10.29 AYY1966 pKa = 10.59 CILIPVKK1973 pKa = 10.51 KK1974 pKa = 9.48 PEE1976 pKa = 4.1 SKK1978 pKa = 9.38 RR1979 pKa = 11.84 TFVGDD1984 pKa = 3.51 LRR1986 pKa = 11.84 DD1987 pKa = 3.52 INFGNNTISMLQLKK2001 pKa = 10.19 NNEE2004 pKa = 3.91 TARR2007 pKa = 11.84 LRR2009 pKa = 11.84 KK2010 pKa = 9.59 SEE2012 pKa = 4.17 LSKK2015 pKa = 9.8 MQYY2018 pKa = 10.51 FEE2020 pKa = 4.7 GPQVNILAINMNALMKK2036 pKa = 10.67 DD2037 pKa = 3.25 RR2038 pKa = 11.84 TLMGLNFDD2046 pKa = 4.79 NLSNMCLTRR2055 pKa = 11.84 LSRR2058 pKa = 11.84 LIDD2061 pKa = 3.82 CDD2063 pKa = 4.07 GSDD2066 pKa = 3.82 SLEE2069 pKa = 4.08 DD2070 pKa = 4.16 SIICFSDD2077 pKa = 3.92 DD2078 pKa = 4.26 PIGQEE2083 pKa = 3.99 EE2084 pKa = 4.34 TQEE2087 pKa = 4.15 MNSIPLFKK2095 pKa = 10.33 VSYY2098 pKa = 8.06 MVSGARR2104 pKa = 11.84 HH2105 pKa = 4.39 VNYY2108 pKa = 10.41 RR2109 pKa = 11.84 NAISQAIYY2117 pKa = 11.13 NEE2119 pKa = 3.74 VTRR2122 pKa = 11.84 FKK2124 pKa = 10.91 KK2125 pKa = 10.67 AFTLQGKK2132 pKa = 7.91 EE2133 pKa = 3.94 FYY2135 pKa = 10.67 NAINKK2140 pKa = 9.13 GMIEE2144 pKa = 4.16 NFICVLNLQPTNVSTKK2160 pKa = 8.16 TLYY2163 pKa = 10.75 DD2164 pKa = 3.84 CFHH2167 pKa = 6.34 VVYY2170 pKa = 10.41 TSDD2173 pKa = 3.59 NKK2175 pKa = 10.99 DD2176 pKa = 3.37 HH2177 pKa = 6.88 EE2178 pKa = 4.49 FHH2180 pKa = 6.86 NLYY2183 pKa = 10.32 LDD2185 pKa = 4.49 SIFFKK2190 pKa = 11.02 NNSPASGEE2198 pKa = 3.9 INYY2201 pKa = 10.12 KK2202 pKa = 9.79 IVKK2205 pKa = 9.51 QFLEE2209 pKa = 4.33 KK2210 pKa = 10.73 FPIPKK2215 pKa = 9.35 RR2216 pKa = 11.84 APWNRR2221 pKa = 11.84 LVRR2224 pKa = 11.84 DD2225 pKa = 4.4 AIEE2228 pKa = 4.24 VGLTKK2233 pKa = 10.4 ARR2235 pKa = 11.84 QQIEE2239 pKa = 4.11 KK2240 pKa = 9.91 CEE2242 pKa = 3.99 PEE2244 pKa = 3.42 IDD2246 pKa = 3.85 FAKK2249 pKa = 10.43 EE2250 pKa = 3.37 LAKK2253 pKa = 10.35 FAEE2256 pKa = 4.68 DD2257 pKa = 3.57 EE2258 pKa = 4.42 EE2259 pKa = 5.25 IIYY2262 pKa = 10.41 EE2263 pKa = 4.12 SEE2265 pKa = 4.16 EE2266 pKa = 3.98 TSGGYY2271 pKa = 10.03 KK2272 pKa = 10.53 DD2273 pKa = 4.52 IDD2275 pKa = 3.2 WAKK2278 pKa = 10.66 IMEE2281 pKa = 4.8 EE2282 pKa = 3.78 EE2283 pKa = 5.36 DD2284 pKa = 3.66 ADD2286 pKa = 3.89 NDD2288 pKa = 3.74
Molecular weight: 266.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.079
IPC2_protein 6.097
IPC_protein 6.16
Toseland 6.465
ProMoST 6.532
Dawson 6.491
Bjellqvist 6.491
Wikipedia 6.504
Rodwell 6.478
Grimsley 6.605
Solomon 6.491
Lehninger 6.478
Nozaki 6.781
DTASelect 6.942
Thurlkill 6.971
EMBOSS 6.927
Sillero 6.868
Patrickios 4.151
IPC_peptide 6.504
IPC2_peptide 6.722
IPC2.peptide.svr19 6.756
Protein with the highest isoelectric point:
>tr|A0A0R7FK74|A0A0R7FK74_9VIRU Replicase OS=Witwatersrand virus OX=1678231 PE=4 SV=1
MM1 pKa = 6.37 QTGAGSLPIPTIRR14 pKa = 11.84 QADD17 pKa = 3.36 ITYY20 pKa = 10.49 GVGDD24 pKa = 5.07 DD25 pKa = 4.44 RR26 pKa = 11.84 PASTFNPEE34 pKa = 3.49 VQYY37 pKa = 11.27 LAFVDD42 pKa = 5.11 RR43 pKa = 11.84 YY44 pKa = 10.28 NQVLLIDD51 pKa = 4.61 NIRR54 pKa = 11.84 SFFLHH59 pKa = 6.7 ARR61 pKa = 11.84 DD62 pKa = 4.88 AKK64 pKa = 11.06 GKK66 pKa = 8.21 MANINKK72 pKa = 10.08 DD73 pKa = 3.84 KK74 pKa = 10.7 ICLNFGTLKK83 pKa = 11.04 LEE85 pKa = 4.08 LVNNYY90 pKa = 9.84 RR91 pKa = 11.84 PGVAKK96 pKa = 10.08 RR97 pKa = 11.84 VVADD101 pKa = 3.74 NEE103 pKa = 4.17 YY104 pKa = 8.78 TLYY107 pKa = 10.73 RR108 pKa = 11.84 ISGYY112 pKa = 8.51 LARR115 pKa = 11.84 FLLDD119 pKa = 4.09 SYY121 pKa = 11.35 NADD124 pKa = 3.71 PAWRR128 pKa = 11.84 DD129 pKa = 3.19 GMKK132 pKa = 10.65 VIVNPISAKK141 pKa = 10.74 LGITWEE147 pKa = 4.51 AGPEE151 pKa = 4.24 VYY153 pKa = 10.63 LGTLPGTEE161 pKa = 3.76 MFLGHH166 pKa = 5.92 FQYY169 pKa = 11.17 YY170 pKa = 9.5 PLAFLILRR178 pKa = 11.84 IKK180 pKa = 10.43 RR181 pKa = 11.84 GEE183 pKa = 4.26 VPPQMASKK191 pKa = 10.76 ALRR194 pKa = 11.84 QRR196 pKa = 11.84 VDD198 pKa = 3.25 GKK200 pKa = 11.28 LSAQWMTEE208 pKa = 3.59 DD209 pKa = 3.39 VQAVKK214 pKa = 9.13 TAVANVEE221 pKa = 3.81 ALKK224 pKa = 10.44 PIFSGISATMSSFLSQFGIRR244 pKa = 11.84 MM245 pKa = 3.68
Molecular weight: 27.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.762
IPC2_protein 8.814
IPC_protein 8.799
Toseland 9.268
ProMoST 9.136
Dawson 9.589
Bjellqvist 9.341
Wikipedia 9.809
Rodwell 9.75
Grimsley 9.692
Solomon 9.648
Lehninger 9.604
Nozaki 9.297
DTASelect 9.326
Thurlkill 9.399
EMBOSS 9.706
Sillero 9.516
Patrickios 4.647
IPC_peptide 9.633
IPC2_peptide 7.951
IPC2.peptide.svr19 7.781
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4092
111
2288
1023.0
117.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.936 ± 0.736
2.688 ± 1.127
5.547 ± 0.49
6.354 ± 0.777
4.643 ± 0.28
4.374 ± 0.673
2.493 ± 0.305
8.382 ± 0.385
7.796 ± 1.031
9.286 ± 0.73
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.59 ± 0.379
5.523 ± 0.617
3.25 ± 0.378
3.959 ± 0.229
4.326 ± 0.843
6.623 ± 0.398
6.867 ± 0.982
4.717 ± 0.356
1.002 ± 0.361
4.643 ± 0.381
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here