Macaca fascicularis papillomavirus 8
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3PU98|C3PU98_RHPV1 Replication protein E1 OS=Macaca fascicularis papillomavirus 8 OX=471186 GN=E1 PE=3 SV=1
MM1 pKa = 7.69 IGPKK5 pKa = 8.52 PTLEE9 pKa = 5.47 DD10 pKa = 2.94 IVLDD14 pKa = 3.9 LTPEE18 pKa = 4.18 PVDD21 pKa = 4.52 LYY23 pKa = 11.22 CEE25 pKa = 4.05 EE26 pKa = 4.17 QLPDD30 pKa = 3.43 SSEE33 pKa = 5.09 DD34 pKa = 3.65 EE35 pKa = 5.31 DD36 pKa = 4.24 EE37 pKa = 5.89 DD38 pKa = 3.98 EE39 pKa = 5.01 VDD41 pKa = 3.43 YY42 pKa = 11.27 HH43 pKa = 7.27 HH44 pKa = 7.64 PARR47 pKa = 11.84 PAEE50 pKa = 4.05 QQCYY54 pKa = 10.09 RR55 pKa = 11.84 IVTSCGGCEE64 pKa = 3.58 SALRR68 pKa = 11.84 LVVVATRR75 pKa = 11.84 DD76 pKa = 3.56 EE77 pKa = 4.16 VRR79 pKa = 11.84 ALHH82 pKa = 6.67 DD83 pKa = 4.28 LLMGTLNIVCPNCAGRR99 pKa = 11.84 AA100 pKa = 3.59
Molecular weight: 11.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 4.177
IPC_protein 4.113
Toseland 3.923
ProMoST 4.24
Dawson 4.075
Bjellqvist 4.228
Wikipedia 3.973
Rodwell 3.948
Grimsley 3.846
Solomon 4.062
Lehninger 4.024
Nozaki 4.19
DTASelect 4.355
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.228
Patrickios 1.036
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.107
Protein with the highest isoelectric point:
>tr|C3PUA2|C3PUA2_RHPV1 Minor capsid protein L2 OS=Macaca fascicularis papillomavirus 8 OX=471186 GN=L2 PE=3 SV=1
TT1 pKa = 6.51 ATILFFVVLFLLLFLVGLVFCARR24 pKa = 11.84 IPSLLLCLSVYY35 pKa = 10.97 ASVLLLMLLFWVTITSPFAAFGLCLFCFLLPLFCIHH71 pKa = 6.64 LHH73 pKa = 5.26 ATSVVYY79 pKa = 9.76 TRR81 pKa = 11.84 LYY83 pKa = 10.38 GG84 pKa = 3.55
Molecular weight: 9.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.714
IPC2_protein 7.337
IPC_protein 7.38
Toseland 6.854
ProMoST 7.834
Dawson 7.98
Bjellqvist 8.375
Wikipedia 7.892
Rodwell 7.966
Grimsley 6.839
Solomon 8.112
Lehninger 8.141
Nozaki 8.726
DTASelect 8.112
Thurlkill 8.141
EMBOSS 8.214
Sillero 8.521
Patrickios 0.006
IPC_peptide 8.097
IPC2_peptide 8.565
IPC2.peptide.svr19 8.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2382
84
636
297.8
33.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.927 ± 0.635
2.981 ± 0.713
5.709 ± 0.52
5.374 ± 0.615
3.317 ± 0.61
6.717 ± 0.415
3.023 ± 0.39
3.778 ± 0.324
4.534 ± 0.832
8.942 ± 1.251
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.386
2.855 ± 0.669
6.759 ± 1.251
4.24 ± 0.8
5.835 ± 0.694
7.179 ± 0.579
6.801 ± 0.478
7.389 ± 0.449
1.553 ± 0.258
3.778 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here