Influenza A virus (A/New York/1051/2006(H1N1))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H1N1 subtype

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2DXR5|X2DXR5_9INFA Polymerase acidic protein OS=Influenza A virus (A/New York/1051/2006(H1N1)) OX=1468612 GN=PA PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.17VEE8 pKa = 4.27TPIRR12 pKa = 11.84NEE14 pKa = 3.68WEE16 pKa = 4.22CRR18 pKa = 11.84CNDD21 pKa = 3.1SSDD24 pKa = 3.94PLVVAASIIGIVHH37 pKa = 7.31LILWIIDD44 pKa = 3.48RR45 pKa = 11.84LFSKK49 pKa = 10.39SIYY52 pKa = 10.07RR53 pKa = 11.84IFKK56 pKa = 9.84HH57 pKa = 5.11GLKK60 pKa = 10.13RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.9EE75 pKa = 4.09YY76 pKa = 10.5RR77 pKa = 11.84EE78 pKa = 3.95EE79 pKa = 3.74QQNAVDD85 pKa = 4.54ADD87 pKa = 3.92DD88 pKa = 4.2DD89 pKa = 4.39HH90 pKa = 6.8FVSIEE95 pKa = 3.9LEE97 pKa = 3.99

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2DZW1|X2DZW1_9INFA Polymerase basic protein 2 OS=Influenza A virus (A/New York/1051/2006(H1N1)) OX=1468612 GN=PB2 PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 6.17RR3 pKa = 11.84IKK5 pKa = 10.52EE6 pKa = 4.01LRR8 pKa = 11.84NLMSQSRR15 pKa = 11.84TRR17 pKa = 11.84EE18 pKa = 3.86ILTKK22 pKa = 8.81TTVDD26 pKa = 2.86HH27 pKa = 5.76MAIIKK32 pKa = 10.06KK33 pKa = 7.84YY34 pKa = 8.29TSGRR38 pKa = 11.84QEE40 pKa = 4.13KK41 pKa = 10.51NPSLRR46 pKa = 11.84MKK48 pKa = 10.18WMMAMKK54 pKa = 10.45YY55 pKa = 10.07PITADD60 pKa = 3.19KK61 pKa = 10.46RR62 pKa = 11.84ITEE65 pKa = 4.55MIPEE69 pKa = 4.33RR70 pKa = 11.84NEE72 pKa = 3.52QGQTLWSKK80 pKa = 10.76MNDD83 pKa = 3.23AGSDD87 pKa = 3.06RR88 pKa = 11.84VMISPLAVTWWNRR101 pKa = 11.84NGPVANTIHH110 pKa = 6.15YY111 pKa = 7.61PKK113 pKa = 9.95IYY115 pKa = 8.56KK116 pKa = 9.62TYY118 pKa = 8.51FEE120 pKa = 4.08KK121 pKa = 11.12VEE123 pKa = 4.08RR124 pKa = 11.84LKK126 pKa = 11.04HH127 pKa = 4.72GTFGPVHH134 pKa = 6.7FRR136 pKa = 11.84NQVKK140 pKa = 9.47IRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84VDD146 pKa = 3.11INPGHH151 pKa = 7.33ADD153 pKa = 3.84LSAKK157 pKa = 9.4EE158 pKa = 3.98AQDD161 pKa = 3.52VIMEE165 pKa = 4.23VVFPNEE171 pKa = 3.21VGARR175 pKa = 11.84ILTSEE180 pKa = 4.22SQLTITKK187 pKa = 9.33EE188 pKa = 4.15KK189 pKa = 10.77KK190 pKa = 10.27EE191 pKa = 3.94EE192 pKa = 3.94LQDD195 pKa = 3.93CKK197 pKa = 10.65ISPLMVAYY205 pKa = 9.39MLEE208 pKa = 4.18RR209 pKa = 11.84EE210 pKa = 4.67LVRR213 pKa = 11.84KK214 pKa = 7.77TRR216 pKa = 11.84FLPVAGGTSSVYY228 pKa = 10.37IEE230 pKa = 4.48VLHH233 pKa = 5.98LTQGTCWEE241 pKa = 4.0QMYY244 pKa = 9.43TPGGEE249 pKa = 4.01VRR251 pKa = 11.84NDD253 pKa = 3.78DD254 pKa = 3.61VDD256 pKa = 3.41QSLIIAARR264 pKa = 11.84NIVRR268 pKa = 11.84RR269 pKa = 11.84AAVSADD275 pKa = 3.35PLASLLEE282 pKa = 4.46MCHH285 pKa = 5.37STQIGGTRR293 pKa = 11.84MVDD296 pKa = 3.19ILRR299 pKa = 11.84QNPTEE304 pKa = 3.98EE305 pKa = 4.05QAVGICKK312 pKa = 9.96AAMGLRR318 pKa = 11.84ISSSFSFGGFTFKK331 pKa = 10.07RR332 pKa = 11.84TSGSSVKK339 pKa = 10.39RR340 pKa = 11.84EE341 pKa = 3.93EE342 pKa = 4.1EE343 pKa = 4.18VLTGNLQTLKK353 pKa = 9.91LTVHH357 pKa = 6.69EE358 pKa = 5.26GYY360 pKa = 10.88EE361 pKa = 4.14EE362 pKa = 3.84FTMVGKK368 pKa = 10.45RR369 pKa = 11.84ATAILKK375 pKa = 9.86KK376 pKa = 9.58ATRR379 pKa = 11.84RR380 pKa = 11.84LIQLIVSGRR389 pKa = 11.84DD390 pKa = 3.17EE391 pKa = 3.98QSIVEE396 pKa = 4.67AIVVAMVFSQEE407 pKa = 3.62DD408 pKa = 3.9CMIKK412 pKa = 10.33AVRR415 pKa = 11.84GDD417 pKa = 3.68LNFVNRR423 pKa = 11.84ANQRR427 pKa = 11.84LNPMHH432 pKa = 6.37QLLRR436 pKa = 11.84HH437 pKa = 5.04FQKK440 pKa = 10.6DD441 pKa = 3.38AKK443 pKa = 10.97VLFLNWGIEE452 pKa = 4.22HH453 pKa = 7.39IDD455 pKa = 3.53NVMGMIGILPDD466 pKa = 3.56MTPSTEE472 pKa = 3.39MSMRR476 pKa = 11.84GVRR479 pKa = 11.84VSKK482 pKa = 10.32MGVDD486 pKa = 3.81EE487 pKa = 4.28YY488 pKa = 11.77SNAEE492 pKa = 4.01RR493 pKa = 11.84VVVSIDD499 pKa = 2.74RR500 pKa = 11.84FLRR503 pKa = 11.84VRR505 pKa = 11.84DD506 pKa = 3.35QRR508 pKa = 11.84GNVLLSPEE516 pKa = 4.55EE517 pKa = 3.94VSEE520 pKa = 4.32TQGTEE525 pKa = 3.64KK526 pKa = 10.4LTITYY531 pKa = 9.91SSSLMWEE538 pKa = 3.85INGPEE543 pKa = 3.84SVLINTYY550 pKa = 7.53QWIIRR555 pKa = 11.84NWEE558 pKa = 3.94TVKK561 pKa = 10.58IQWSQNPTMLYY572 pKa = 10.39NKK574 pKa = 9.62MEE576 pKa = 4.24FEE578 pKa = 4.66PFQSLVPKK586 pKa = 10.37AIRR589 pKa = 11.84GQYY592 pKa = 9.91SGFVRR597 pKa = 11.84TLFQQMRR604 pKa = 11.84DD605 pKa = 3.48VLGTFDD611 pKa = 4.48TIQIIKK617 pKa = 10.05LLPFAAAPPKK627 pKa = 9.4QSRR630 pKa = 11.84MQFSSLTVNVRR641 pKa = 11.84GSGMRR646 pKa = 11.84ILVRR650 pKa = 11.84GNSPVFNYY658 pKa = 10.84NKK660 pKa = 6.21TTKK663 pKa = 10.53RR664 pKa = 11.84LTILGKK670 pKa = 10.12DD671 pKa = 3.16AGTLTEE677 pKa = 5.51DD678 pKa = 4.39PDD680 pKa = 3.51EE681 pKa = 4.55GTAGVEE687 pKa = 4.29SAVLRR692 pKa = 11.84GFLILGKK699 pKa = 9.19EE700 pKa = 3.99DD701 pKa = 3.41RR702 pKa = 11.84RR703 pKa = 11.84YY704 pKa = 11.01GPALSINEE712 pKa = 4.34LSNLAKK718 pKa = 10.63GEE720 pKa = 4.09KK721 pKa = 10.32ANVLIGQGDD730 pKa = 3.9VVLVMKK736 pKa = 10.39RR737 pKa = 11.84KK738 pKa = 9.29RR739 pKa = 11.84DD740 pKa = 3.62SSILTDD746 pKa = 3.31SQTATKK752 pKa = 9.73RR753 pKa = 11.84IRR755 pKa = 11.84MAINN759 pKa = 3.15

Molecular weight: 85.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

4774

57

759

397.8

45.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.781 ± 0.442

1.78 ± 0.357

4.608 ± 0.284

7.457 ± 0.679

4.043 ± 0.283

6.556 ± 0.483

1.801 ± 0.166

6.598 ± 0.324

6.116 ± 0.306

7.897 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.729 ± 0.466

5.383 ± 0.463

3.812 ± 0.159

3.833 ± 0.421

6.473 ± 0.664

7.52 ± 0.455

6.535 ± 0.291

5.656 ± 0.441

1.592 ± 0.216

2.828 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski