Malvastrum yellow vein Yunnan virus
Average proteome isoelectric point is 7.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5K2L6|Q5K2L6_9GEMI Protein V2 OS=Malvastrum yellow vein Yunnan virus OX=290030 GN=AV2 PE=3 SV=1
MM1 pKa = 8.06 WDD3 pKa = 3.35 PLLNEE8 pKa = 4.38 FPEE11 pKa = 4.68 TVHH14 pKa = 6.85 GFRR17 pKa = 11.84 CMLSIKK23 pKa = 9.95 YY24 pKa = 9.47 LQLLSEE30 pKa = 5.34 GYY32 pKa = 10.54 SPDD35 pKa = 3.26 TVGFDD40 pKa = 5.35 LIRR43 pKa = 11.84 EE44 pKa = 4.7 LISILRR50 pKa = 11.84 SRR52 pKa = 11.84 NYY54 pKa = 9.91 VEE56 pKa = 4.26 ASCRR60 pKa = 11.84 YY61 pKa = 7.49 RR62 pKa = 11.84 HH63 pKa = 6.16 FYY65 pKa = 10.85 SRR67 pKa = 11.84 VEE69 pKa = 4.18 GASSSEE75 pKa = 3.66 LRR77 pKa = 11.84 QPLLNPCSCPHH88 pKa = 6.58 CPRR91 pKa = 11.84 HH92 pKa = 6.31 KK93 pKa = 9.94 ISKK96 pKa = 9.81 LGEE99 pKa = 3.91 QAHH102 pKa = 6.52 EE103 pKa = 4.16 SQAQNVQDD111 pKa = 3.62 VV112 pKa = 3.43
Molecular weight: 12.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.035
IPC2_protein 6.084
IPC_protein 6.211
Toseland 6.415
ProMoST 6.605
Dawson 6.576
Bjellqvist 6.504
Wikipedia 6.59
Rodwell 6.561
Grimsley 6.517
Solomon 6.576
Lehninger 6.576
Nozaki 6.825
DTASelect 7.0
Thurlkill 7.029
EMBOSS 7.015
Sillero 6.971
Patrickios 3.63
IPC_peptide 6.59
IPC2_peptide 6.912
IPC2.peptide.svr19 6.785
Protein with the highest isoelectric point:
>tr|Q5K2L6|Q5K2L6_9GEMI Protein V2 OS=Malvastrum yellow vein Yunnan virus OX=290030 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.5 IVISTPGSKK16 pKa = 9.65 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.11 SPYY27 pKa = 8.11 STRR30 pKa = 11.84 AAVPTVRR37 pKa = 11.84 VTKK40 pKa = 10.43 SRR42 pKa = 11.84 SWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.57 RR60 pKa = 11.84 SPDD63 pKa = 3.29 VPKK66 pKa = 10.81 GCEE69 pKa = 4.39 GPCKK73 pKa = 10.37 VQSFDD78 pKa = 3.26 AKK80 pKa = 11.06 NDD82 pKa = 3.27 IGHH85 pKa = 6.11 MGKK88 pKa = 10.22 VICLSDD94 pKa = 3.33 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.78 RR108 pKa = 11.84 FCVKK112 pKa = 10.05 SLYY115 pKa = 10.31 FVGKK119 pKa = 9.14 IWMDD123 pKa = 3.34 EE124 pKa = 4.02 NIKK127 pKa = 10.41 VKK129 pKa = 10.66 NHH131 pKa = 5.45 TNTVLFWIVRR141 pKa = 11.84 DD142 pKa = 3.85 RR143 pKa = 11.84 RR144 pKa = 11.84 PTGTPYY150 pKa = 11.16 DD151 pKa = 3.75 FQQVFNVYY159 pKa = 10.67 DD160 pKa = 4.09 NEE162 pKa = 4.37 PSTATVKK169 pKa = 10.59 NDD171 pKa = 3.0 QRR173 pKa = 11.84 DD174 pKa = 3.37 RR175 pKa = 11.84 FQVIRR180 pKa = 11.84 RR181 pKa = 11.84 FQATVTGGQYY191 pKa = 10.18 AAKK194 pKa = 8.87 EE195 pKa = 3.58 QAIIRR200 pKa = 11.84 KK201 pKa = 8.88 FYY203 pKa = 10.48 RR204 pKa = 11.84 VNNYY208 pKa = 7.79 VVYY211 pKa = 10.32 NHH213 pKa = 6.0 QEE215 pKa = 3.43 AGKK218 pKa = 10.41 YY219 pKa = 8.39 EE220 pKa = 4.09 NHH222 pKa = 6.52 TEE224 pKa = 3.99 NALLLYY230 pKa = 7.29 MACTHH235 pKa = 7.07 ASNPVYY241 pKa = 9.99 ATLKK245 pKa = 8.67 VRR247 pKa = 11.84 SYY249 pKa = 10.98 FYY251 pKa = 11.25 DD252 pKa = 3.13 SVTNN256 pKa = 3.98
Molecular weight: 29.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.575
IPC_protein 9.853
Toseland 10.087
ProMoST 9.853
Dawson 10.321
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.643
Grimsley 10.409
Solomon 10.35
Lehninger 10.306
Nozaki 10.116
DTASelect 10.028
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.233
Patrickios 10.058
IPC_peptide 10.35
IPC2_peptide 8.931
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1112
97
363
185.3
21.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.216 ± 0.938
2.248 ± 0.404
4.586 ± 0.417
4.946 ± 0.905
4.586 ± 0.373
4.946 ± 0.296
3.507 ± 0.611
5.755 ± 0.942
5.935 ± 0.477
7.014 ± 1.208
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.625
6.205 ± 0.71
5.845 ± 0.476
5.126 ± 0.611
6.655 ± 1.134
9.083 ± 1.621
5.216 ± 0.961
5.486 ± 1.485
1.439 ± 0.206
3.957 ± 0.654
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here