Streptococcus phage IPP26
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5SAM3|A0A1S5SAM3_9CAUD Putative helicase OS=Streptococcus phage IPP26 OX=1916166 GN=IPP26_00021 PE=4 SV=1
MM1 pKa = 7.36 TKK3 pKa = 10.42 SDD5 pKa = 3.89 LGPYY9 pKa = 9.9 LDD11 pKa = 4.98 AVTNEE16 pKa = 4.42 DD17 pKa = 3.65 GTLLICKK24 pKa = 8.59 TEE26 pKa = 3.77 QGAYY30 pKa = 9.48 IGDD33 pKa = 4.28 FNPSCDD39 pKa = 3.67 EE40 pKa = 3.84 EE41 pKa = 5.78 DD42 pKa = 3.78 FVLTYY47 pKa = 11.02 EE48 pKa = 4.44 DD49 pKa = 3.86 VSVSLSYY56 pKa = 11.13 AQVLSATLLKK66 pKa = 10.38 II67 pKa = 4.01
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A1S5SAH4|A0A1S5SAH4_9CAUD Uncharacterized protein OS=Streptococcus phage IPP26 OX=1916166 GN=IPP26_00038 PE=4 SV=1
MM1 pKa = 7.43 EE2 pKa = 5.29 SVLEE6 pKa = 4.05 SLKK9 pKa = 10.25 QEE11 pKa = 4.06 KK12 pKa = 10.33 DD13 pKa = 3.25 HH14 pKa = 6.71 LEE16 pKa = 4.25 KK17 pKa = 10.18 IIKK20 pKa = 9.83 VVTSGGKK27 pKa = 8.88 FLRR30 pKa = 11.84 LPYY33 pKa = 9.68 QKK35 pKa = 10.31 KK36 pKa = 9.98 SRR38 pKa = 11.84 SISEE42 pKa = 3.93 NLKK45 pKa = 10.79 LISQNLDD52 pKa = 3.19 RR53 pKa = 11.84 LSCLYY58 pKa = 9.73 NQRR61 pKa = 11.84 GEE63 pKa = 4.1 RR64 pKa = 11.84 MTDD67 pKa = 2.94 RR68 pKa = 11.84 EE69 pKa = 4.41 LFKK72 pKa = 11.19 LPEE75 pKa = 5.07 DD76 pKa = 3.92 YY77 pKa = 11.19 VEE79 pKa = 5.21 SSGLDD84 pKa = 3.03 KK85 pKa = 10.55 ITFEE89 pKa = 4.61 VPFEE93 pKa = 4.39 LFTKK97 pKa = 9.81 ILKK100 pKa = 10.64 GYY102 pKa = 6.8 GHH104 pKa = 7.13 KK105 pKa = 10.43 LAWEE109 pKa = 4.59 DD110 pKa = 3.46 FRR112 pKa = 11.84 QIKK115 pKa = 9.14 IHH117 pKa = 6.69 PSTRR121 pKa = 11.84 TKK123 pKa = 9.85 KK124 pKa = 8.92 TVGQCQFLFSIWMNDD139 pKa = 3.16 HH140 pKa = 7.16 LKK142 pKa = 10.36 PAIKK146 pKa = 10.04 SSKK149 pKa = 9.19 EE150 pKa = 3.8 LQKK153 pKa = 11.19 EE154 pKa = 4.11 PVRR157 pKa = 11.84 KK158 pKa = 7.89 TKK160 pKa = 9.74 KK161 pKa = 9.56 QKK163 pKa = 10.13 RR164 pKa = 11.84 LHH166 pKa = 6.56 KK167 pKa = 10.14 LAQNLLHH174 pKa = 6.77 RR175 pKa = 11.84 MKK177 pKa = 11.04 GG178 pKa = 3.34
Molecular weight: 21.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.991
IPC2_protein 9.194
IPC_protein 9.077
Toseland 10.218
ProMoST 9.692
Dawson 10.321
Bjellqvist 9.897
Wikipedia 10.409
Rodwell 11.038
Grimsley 10.35
Solomon 10.335
Lehninger 10.321
Nozaki 10.189
DTASelect 9.882
Thurlkill 10.204
EMBOSS 10.584
Sillero 10.233
Patrickios 10.76
IPC_peptide 10.35
IPC2_peptide 8.287
IPC2.peptide.svr19 8.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10549
43
873
206.8
23.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.446 ± 0.401
0.502 ± 0.087
6.56 ± 0.314
7.802 ± 0.33
4.019 ± 0.199
5.754 ± 0.435
1.469 ± 0.142
7.261 ± 0.302
9.243 ± 0.424
8.759 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.626 ± 0.208
5.707 ± 0.241
2.522 ± 0.165
3.811 ± 0.168
4.133 ± 0.331
6.2 ± 0.233
6.114 ± 0.209
6.105 ± 0.297
1.138 ± 0.161
3.83 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here