Streptococcus phage IPP26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S5SAM3|A0A1S5SAM3_9CAUD Putative helicase OS=Streptococcus phage IPP26 OX=1916166 GN=IPP26_00021 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.42SDD5 pKa = 3.89LGPYY9 pKa = 9.9LDD11 pKa = 4.98AVTNEE16 pKa = 4.42DD17 pKa = 3.65GTLLICKK24 pKa = 8.59TEE26 pKa = 3.77QGAYY30 pKa = 9.48IGDD33 pKa = 4.28FNPSCDD39 pKa = 3.67EE40 pKa = 3.84EE41 pKa = 5.78DD42 pKa = 3.78FVLTYY47 pKa = 11.02EE48 pKa = 4.44DD49 pKa = 3.86VSVSLSYY56 pKa = 11.13AQVLSATLLKK66 pKa = 10.38II67 pKa = 4.01

Molecular weight:
7.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S5SAH4|A0A1S5SAH4_9CAUD Uncharacterized protein OS=Streptococcus phage IPP26 OX=1916166 GN=IPP26_00038 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.29SVLEE6 pKa = 4.05SLKK9 pKa = 10.25QEE11 pKa = 4.06KK12 pKa = 10.33DD13 pKa = 3.25HH14 pKa = 6.71LEE16 pKa = 4.25KK17 pKa = 10.18IIKK20 pKa = 9.83VVTSGGKK27 pKa = 8.88FLRR30 pKa = 11.84LPYY33 pKa = 9.68QKK35 pKa = 10.31KK36 pKa = 9.98SRR38 pKa = 11.84SISEE42 pKa = 3.93NLKK45 pKa = 10.79LISQNLDD52 pKa = 3.19RR53 pKa = 11.84LSCLYY58 pKa = 9.73NQRR61 pKa = 11.84GEE63 pKa = 4.1RR64 pKa = 11.84MTDD67 pKa = 2.94RR68 pKa = 11.84EE69 pKa = 4.41LFKK72 pKa = 11.19LPEE75 pKa = 5.07DD76 pKa = 3.92YY77 pKa = 11.19VEE79 pKa = 5.21SSGLDD84 pKa = 3.03KK85 pKa = 10.55ITFEE89 pKa = 4.61VPFEE93 pKa = 4.39LFTKK97 pKa = 9.81ILKK100 pKa = 10.64GYY102 pKa = 6.8GHH104 pKa = 7.13KK105 pKa = 10.43LAWEE109 pKa = 4.59DD110 pKa = 3.46FRR112 pKa = 11.84QIKK115 pKa = 9.14IHH117 pKa = 6.69PSTRR121 pKa = 11.84TKK123 pKa = 9.85KK124 pKa = 8.92TVGQCQFLFSIWMNDD139 pKa = 3.16HH140 pKa = 7.16LKK142 pKa = 10.36PAIKK146 pKa = 10.04SSKK149 pKa = 9.19EE150 pKa = 3.8LQKK153 pKa = 11.19EE154 pKa = 4.11PVRR157 pKa = 11.84KK158 pKa = 7.89TKK160 pKa = 9.74KK161 pKa = 9.56QKK163 pKa = 10.13RR164 pKa = 11.84LHH166 pKa = 6.56KK167 pKa = 10.14LAQNLLHH174 pKa = 6.77RR175 pKa = 11.84MKK177 pKa = 11.04GG178 pKa = 3.34

Molecular weight:
21.0 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10549

43

873

206.8

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.446 ± 0.401

0.502 ± 0.087

6.56 ± 0.314

7.802 ± 0.33

4.019 ± 0.199

5.754 ± 0.435

1.469 ± 0.142

7.261 ± 0.302

9.243 ± 0.424

8.759 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.208

5.707 ± 0.241

2.522 ± 0.165

3.811 ± 0.168

4.133 ± 0.331

6.2 ± 0.233

6.114 ± 0.209

6.105 ± 0.297

1.138 ± 0.161

3.83 ± 0.252

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski