Rhizobium sp. BK696

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7SCN8|A0A4Q7SCN8_9RHIZ ATPase subunit of ABC transporter with duplicated ATPase domains OS=Rhizobium sp. BK696 OX=2512157 GN=EV569_4752 PE=3 SV=1
MM1 pKa = 6.43TTSIVNAVGRR11 pKa = 11.84PLYY14 pKa = 10.81YY15 pKa = 10.34SGSSTAWFSATKK27 pKa = 10.2AGPVQAGTAGNDD39 pKa = 3.82SMWGDD44 pKa = 3.83SAVKK48 pKa = 10.48VSMQGGTGDD57 pKa = 4.58DD58 pKa = 2.84IYY60 pKa = 11.73YY61 pKa = 10.51LYY63 pKa = 10.43SAKK66 pKa = 9.13NTAVEE71 pKa = 4.18QADD74 pKa = 3.95SGTDD78 pKa = 3.67TINTWMDD85 pKa = 3.03YY86 pKa = 9.34TLPDD90 pKa = 3.34NFEE93 pKa = 4.33NLTVTGNGRR102 pKa = 11.84YY103 pKa = 9.81AFGNNADD110 pKa = 4.47NIISGASGGQTIDD123 pKa = 3.13GGAGNDD129 pKa = 3.49VLIGGGGADD138 pKa = 2.77TFVFNHH144 pKa = 6.52GSGSDD149 pKa = 4.03LITDD153 pKa = 4.34FGADD157 pKa = 3.28DD158 pKa = 4.79VIRR161 pKa = 11.84MDD163 pKa = 4.05SFSSFADD170 pKa = 3.81LVAHH174 pKa = 6.03STQEE178 pKa = 4.17GADD181 pKa = 3.55VRR183 pKa = 11.84IDD185 pKa = 3.53TGNGEE190 pKa = 4.55SLVLANADD198 pKa = 3.18IDD200 pKa = 4.02QLSAAQFQLTLDD212 pKa = 3.81RR213 pKa = 11.84SHH215 pKa = 7.37LSLSFNDD222 pKa = 4.4DD223 pKa = 3.7FNSLSLRR230 pKa = 11.84DD231 pKa = 3.89GDD233 pKa = 4.56QGTWDD238 pKa = 3.83AKK240 pKa = 10.31FWWAPEE246 pKa = 3.93RR247 pKa = 11.84GGTLSGNGEE256 pKa = 4.23QQWYY260 pKa = 9.56INPSYY265 pKa = 10.62EE266 pKa = 4.16PTASASPFSVNDD278 pKa = 4.86GILTISANPASAALQQQIDD297 pKa = 4.4GYY299 pKa = 10.59DD300 pKa = 3.59YY301 pKa = 11.44TSGLLNTHH309 pKa = 6.12SSFSQTYY316 pKa = 8.43GYY318 pKa = 10.83FEE320 pKa = 4.72IRR322 pKa = 11.84ADD324 pKa = 3.65MPDD327 pKa = 3.83DD328 pKa = 3.62QGVWPAFWLLPEE340 pKa = 5.2DD341 pKa = 4.82GSWPPEE347 pKa = 3.78LDD349 pKa = 3.52VVEE352 pKa = 5.06MRR354 pKa = 11.84GQDD357 pKa = 3.55PNTVNVTVHH366 pKa = 6.41SDD368 pKa = 3.25DD369 pKa = 4.36GGSHH373 pKa = 7.29IIDD376 pKa = 3.56STPVKK381 pKa = 10.24VASTEE386 pKa = 3.97GFHH389 pKa = 7.13NYY391 pKa = 8.37GVLWDD396 pKa = 4.2EE397 pKa = 5.65DD398 pKa = 4.25HH399 pKa = 6.3ITWYY403 pKa = 10.65FDD405 pKa = 3.54DD406 pKa = 4.12VAVAQADD413 pKa = 4.36TPDD416 pKa = 4.22DD417 pKa = 3.78MHH419 pKa = 8.67DD420 pKa = 3.45PMYY423 pKa = 10.32MLVNLAVGGAAGSPADD439 pKa = 3.72GLAGGSEE446 pKa = 4.06MNIDD450 pKa = 3.96YY451 pKa = 10.73IRR453 pKa = 11.84AYY455 pKa = 9.74EE456 pKa = 4.08LADD459 pKa = 3.2NTVPPEE465 pKa = 3.98PAVEE469 pKa = 4.7FYY471 pKa = 11.19VV472 pKa = 3.62

Molecular weight: 50.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7T0M4|A0A4Q7T0M4_9RHIZ Uncharacterized protein OS=Rhizobium sp. BK696 OX=2512157 GN=EV569_0170 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGQKK29 pKa = 9.87VLSARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6267

0

6267

1901983

29

2960

303.5

32.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.003 ± 0.037

0.777 ± 0.01

5.758 ± 0.026

5.748 ± 0.03

3.948 ± 0.02

8.304 ± 0.034

1.989 ± 0.015

5.798 ± 0.025

3.891 ± 0.024

9.848 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.016

2.888 ± 0.019

4.802 ± 0.02

3.144 ± 0.02

6.428 ± 0.032

5.937 ± 0.026

5.32 ± 0.025

7.222 ± 0.026

1.267 ± 0.013

2.343 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski