Anaerospora hongkongensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Anaerospora

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4497 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1PXY2|A0A4R1PXY2_9FIRM Uncharacterized protein OS=Anaerospora hongkongensis OX=244830 GN=EV210_10564 PE=4 SV=1
MM1 pKa = 7.59GFFDD5 pKa = 6.51DD6 pKa = 3.19ICNRR10 pKa = 11.84HH11 pKa = 4.58WCEE14 pKa = 4.5EE15 pKa = 4.34LEE17 pKa = 4.41CSCGINVVIFTADD30 pKa = 2.87GFAFFGLLDD39 pKa = 3.72RR40 pKa = 11.84VEE42 pKa = 4.72DD43 pKa = 4.68GIVILLPAAGEE54 pKa = 4.12NEE56 pKa = 4.31VFILTPGGDD65 pKa = 3.34VVTEE69 pKa = 3.81EE70 pKa = 3.99LSYY73 pKa = 11.06IEE75 pKa = 4.93LCTIVALSKK84 pKa = 11.04NVIGNPFEE92 pKa = 5.22EE93 pKa = 5.25DD94 pKa = 3.11DD95 pKa = 4.14CEE97 pKa = 4.92RR98 pKa = 11.84GGKK101 pKa = 8.98

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1Q3W3|A0A4R1Q3W3_9FIRM Nitrogen fixation NifU-like protein OS=Anaerospora hongkongensis OX=244830 GN=EV210_103191 PE=4 SV=1
MM1 pKa = 6.97KK2 pKa = 9.85QKK4 pKa = 11.44SMMALRR10 pKa = 11.84TIACGWLAFLCSFVDD25 pKa = 3.63RR26 pKa = 11.84LSWPPIIPMAAVEE39 pKa = 4.3LRR41 pKa = 11.84LTAAEE46 pKa = 4.31AGGFMSAFFFGYY58 pKa = 10.75LLTQLPGGMLADD70 pKa = 3.23RR71 pKa = 11.84WGTRR75 pKa = 11.84KK76 pKa = 9.79VLLVSLFVMGLFTMGLAVTNDD97 pKa = 3.58FGQGVILRR105 pKa = 11.84FLAGFGSGAVLAAAVKK121 pKa = 10.24GVYY124 pKa = 10.06DD125 pKa = 3.79HH126 pKa = 6.86VAAEE130 pKa = 4.08HH131 pKa = 6.58RR132 pKa = 11.84AMAMGFFMTSAPLGLLLANLLSPLIAAHH160 pKa = 5.57YY161 pKa = 8.94GWRR164 pKa = 11.84TSFLFAGSMTLAALGLTWLILPRR187 pKa = 11.84RR188 pKa = 11.84TGLRR192 pKa = 11.84IVPAAGKK199 pKa = 8.43TGIKK203 pKa = 10.22FEE205 pKa = 4.42LKK207 pKa = 10.28EE208 pKa = 4.14IITNRR213 pKa = 11.84EE214 pKa = 3.7LMLTAAAGFFAMWGTWGTLTWANAYY239 pKa = 7.4MHH241 pKa = 6.46QGLGLSLKK249 pKa = 10.42EE250 pKa = 4.3SGRR253 pKa = 11.84TMALFGIGALIGQPVVGWLSDD274 pKa = 3.23RR275 pKa = 11.84FRR277 pKa = 11.84QRR279 pKa = 11.84RR280 pKa = 11.84RR281 pKa = 11.84QASMVLLGVFAVLLWLFAKK300 pKa = 10.59NQDD303 pKa = 3.44AQYY306 pKa = 10.76LAVLATLLGAGAFMFGPVLNTFISEE331 pKa = 4.14LVAARR336 pKa = 11.84SVATAVGFCNGVWQIGSLISPVTAGLVLDD365 pKa = 3.75QTGSYY370 pKa = 10.54VYY372 pKa = 10.77AFSILAAGPLVAVGLLAFVRR392 pKa = 11.84SDD394 pKa = 2.94RR395 pKa = 11.84AFVEE399 pKa = 4.46RR400 pKa = 11.84PLTKK404 pKa = 10.46ARR406 pKa = 11.84IQAAGSLPGPVPKK419 pKa = 10.63

Molecular weight: 44.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4497

0

4497

1371066

29

4707

304.9

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.646 ± 0.051

1.186 ± 0.018

4.76 ± 0.026

6.193 ± 0.036

3.813 ± 0.029

7.619 ± 0.045

1.877 ± 0.016

7.232 ± 0.036

5.505 ± 0.03

9.992 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.021

3.826 ± 0.029

3.927 ± 0.023

4.074 ± 0.026

4.693 ± 0.031

5.605 ± 0.028

5.649 ± 0.041

7.499 ± 0.036

1.003 ± 0.014

3.218 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski