Wenling tonguesole paramyxovirus
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1GMZ7|A0A2P1GMZ7_9MONO Uncharacterized protein OS=Wenling tonguesole paramyxovirus OX=2116454 PE=4 SV=1
MM1 pKa = 8.04 DD2 pKa = 4.85 WGDD5 pKa = 3.66 EE6 pKa = 4.08 QQTEE10 pKa = 4.22 DD11 pKa = 3.55 MFKK14 pKa = 10.06 IGKK17 pKa = 8.38 DD18 pKa = 3.35 LKK20 pKa = 11.16 VADD23 pKa = 3.97 QMLSVNTHH31 pKa = 6.94 DD32 pKa = 4.18 SAEE35 pKa = 4.38 DD36 pKa = 3.74 YY37 pKa = 11.19 ALSTSGVRR45 pKa = 11.84 KK46 pKa = 9.87 ILDD49 pKa = 3.4 KK50 pKa = 11.42 SGLTVTGSALLKK62 pKa = 10.15 PGAKK66 pKa = 8.54 STVISLFEE74 pKa = 4.31 PLEE77 pKa = 4.13 MAVSPEE83 pKa = 3.94 PVQLVYY89 pKa = 10.65 DD90 pKa = 4.03 IPDD93 pKa = 3.29 EE94 pKa = 4.72 RR95 pKa = 11.84 DD96 pKa = 3.42 TIKK99 pKa = 8.12 TTIEE103 pKa = 4.08 DD104 pKa = 4.15 PEE106 pKa = 4.74 PNALLGEE113 pKa = 4.18 IKK115 pKa = 10.29 KK116 pKa = 9.77 IQATQSQHH124 pKa = 4.69 TSMLNEE130 pKa = 4.89 IIQKK134 pKa = 10.07 ISTQDD139 pKa = 3.49 SILKK143 pKa = 9.78 SISSISTQISILSTLLEE160 pKa = 4.98 KK161 pKa = 10.09 GTIMCANPALPDD173 pKa = 3.55 QQSTRR178 pKa = 11.84 LYY180 pKa = 10.8 GATLEE185 pKa = 4.26 EE186 pKa = 3.93 NRR188 pKa = 11.84 QEE190 pKa = 4.3 KK191 pKa = 8.37 MHH193 pKa = 6.49 NLASRR198 pKa = 11.84 APMFGALSEE207 pKa = 4.05 ALKK210 pKa = 11.21 NKK212 pKa = 8.87 GKK214 pKa = 9.52 PLIGYY219 pKa = 7.28 GEE221 pKa = 4.36 PSRR224 pKa = 11.84 GSIMEE229 pKa = 5.25 RR230 pKa = 11.84 IPILLKK236 pKa = 10.61 GLKK239 pKa = 8.51 TSSRR243 pKa = 11.84 NEE245 pKa = 3.73 YY246 pKa = 8.89 TSQYY250 pKa = 10.16 VNRR253 pKa = 11.84 DD254 pKa = 3.21 DD255 pKa = 5.39 SFDD258 pKa = 5.06 PIQFSAEE265 pKa = 4.02 LEE267 pKa = 4.2 QVLSQEE273 pKa = 4.14 QADD276 pKa = 3.75 ISTEE280 pKa = 3.78
Molecular weight: 30.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.832
IPC2_protein 4.774
IPC_protein 4.685
Toseland 4.546
ProMoST 4.749
Dawson 4.635
Bjellqvist 4.8
Wikipedia 4.495
Rodwell 4.533
Grimsley 4.457
Solomon 4.635
Lehninger 4.584
Nozaki 4.736
DTASelect 4.889
Thurlkill 4.546
EMBOSS 4.52
Sillero 4.8
Patrickios 4.24
IPC_peptide 4.635
IPC2_peptide 4.8
IPC2.peptide.svr19 4.733
Protein with the highest isoelectric point:
>tr|A0A2P1GMZ6|A0A2P1GMZ6_9MONO Uncharacterized protein OS=Wenling tonguesole paramyxovirus OX=2116454 PE=4 SV=1
MM1 pKa = 7.3 EE2 pKa = 5.06 VDD4 pKa = 4.84 LGRR7 pKa = 11.84 LPYY10 pKa = 10.25 DD11 pKa = 3.2 HH12 pKa = 7.74 PGNDD16 pKa = 3.46 RR17 pKa = 11.84 DD18 pKa = 4.0 KK19 pKa = 10.43 LTGRR23 pKa = 11.84 TGQLVLSPYY32 pKa = 10.84 PNMTNDD38 pKa = 2.82 RR39 pKa = 11.84 VAIFVIGFVYY49 pKa = 10.24 TGDD52 pKa = 3.21 KK53 pKa = 10.32 PAFNEE58 pKa = 4.33 GEE60 pKa = 4.2 KK61 pKa = 10.25 LAKK64 pKa = 10.19 ARR66 pKa = 11.84 FPLGVHH72 pKa = 5.78 NRR74 pKa = 11.84 SDD76 pKa = 4.8 DD77 pKa = 3.63 FDD79 pKa = 5.96 SYY81 pKa = 11.87 TKK83 pKa = 10.65 GLKK86 pKa = 10.32 NSVFTVTRR94 pKa = 11.84 VMRR97 pKa = 11.84 KK98 pKa = 8.39 VEE100 pKa = 4.14 YY101 pKa = 10.12 VALFPEE107 pKa = 4.56 YY108 pKa = 9.84 LDD110 pKa = 4.32 PGIAPLYY117 pKa = 9.26 PRR119 pKa = 11.84 GKK121 pKa = 8.6 RR122 pKa = 11.84 TLMYY126 pKa = 10.14 DD127 pKa = 2.9 ARR129 pKa = 11.84 KK130 pKa = 9.05 FFPDD134 pKa = 2.59 IHH136 pKa = 7.71 HH137 pKa = 7.01 FEE139 pKa = 4.16 MNAPLKK145 pKa = 10.57 CKK147 pKa = 9.21 VVCVIYY153 pKa = 9.6 TKK155 pKa = 10.35 IPSAVKK161 pKa = 9.06 IWKK164 pKa = 7.38 PTFFSKK170 pKa = 10.99 VEE172 pKa = 4.04 SLPLVAMKK180 pKa = 10.33 LVVEE184 pKa = 4.37 IQLGEE189 pKa = 4.04 IDD191 pKa = 3.71 QGTAFTSHH199 pKa = 5.72 IQIPLGWKK207 pKa = 9.19 KK208 pKa = 11.02 GKK210 pKa = 10.59 GDD212 pKa = 5.03 LEE214 pKa = 6.35 DD215 pKa = 4.5 DD216 pKa = 4.62 LKK218 pKa = 11.48 DD219 pKa = 3.5 ALITIRR225 pKa = 11.84 DD226 pKa = 3.74 AAPKK230 pKa = 9.13 VNLGAVHH237 pKa = 7.26 GISLHH242 pKa = 6.26 VMLTGQVSKK251 pKa = 10.84 SIKK254 pKa = 10.23 AYY256 pKa = 10.85 LLGKK260 pKa = 9.38 SAIVMSVADD269 pKa = 3.98 TCPSVNFEE277 pKa = 4.76 LCTGSISIKK286 pKa = 9.24 SVKK289 pKa = 10.26 ACATPTIPATAVLINSATEE308 pKa = 3.73 DD309 pKa = 3.94 LEE311 pKa = 4.23 NHH313 pKa = 6.71 AEE315 pKa = 4.12 KK316 pKa = 10.44 QKK318 pKa = 11.16 KK319 pKa = 8.44 NRR321 pKa = 3.7
Molecular weight: 35.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.455
IPC2_protein 8.331
IPC_protein 8.229
Toseland 8.902
ProMoST 8.873
Dawson 9.224
Bjellqvist 9.092
Wikipedia 9.326
Rodwell 9.472
Grimsley 9.18
Solomon 9.297
Lehninger 9.282
Nozaki 9.268
DTASelect 8.975
Thurlkill 9.136
EMBOSS 9.385
Sillero 9.297
Patrickios 4.8
IPC_peptide 9.297
IPC2_peptide 7.717
IPC2.peptide.svr19 7.717
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
4415
110
2125
490.6
54.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.614 ± 0.812
1.789 ± 0.261
5.776 ± 0.346
4.96 ± 0.676
3.42 ± 0.344
5.685 ± 0.401
2.559 ± 0.357
7.746 ± 0.532
5.232 ± 0.576
10.51 ± 0.61
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.899 ± 0.356
4.643 ± 0.348
4.168 ± 0.309
3.692 ± 0.327
5.3 ± 0.572
7.407 ± 0.554
7.61 ± 0.59
5.798 ± 0.552
1.427 ± 0.382
2.763 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here