Mycobacterium phage Mercurio

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TCZ0|A0A5J6TCZ0_9CAUD Uncharacterized protein OS=Mycobacterium phage Mercurio OX=2575612 GN=64 PE=4 SV=1
MM1 pKa = 6.76STSPAMRR8 pKa = 11.84TALDD12 pKa = 3.91ALDD15 pKa = 3.96AAIVDD20 pKa = 3.75VFEE23 pKa = 5.99AIRR26 pKa = 11.84SDD28 pKa = 3.68APEE31 pKa = 4.68DD32 pKa = 3.99TPADD36 pKa = 5.07AIPVDD41 pKa = 4.02AVLLIGVQHH50 pKa = 7.0IDD52 pKa = 3.01SDD54 pKa = 3.87DD55 pKa = 3.69DD56 pKa = 3.69ARR58 pKa = 11.84IGYY61 pKa = 8.93VEE63 pKa = 3.9VVPRR67 pKa = 11.84HH68 pKa = 5.46GAQPAYY74 pKa = 8.56ITRR77 pKa = 11.84GLLDD81 pKa = 4.29DD82 pKa = 4.57ARR84 pKa = 11.84HH85 pKa = 6.42LIDD88 pKa = 5.14QIADD92 pKa = 3.55QGDD95 pKa = 3.8EE96 pKa = 4.37TPP98 pKa = 4.5

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TA38|A0A5J6TA38_9CAUD Uncharacterized protein OS=Mycobacterium phage Mercurio OX=2575612 GN=50 PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84HH4 pKa = 6.13PSQGGPYY11 pKa = 9.97GRR13 pKa = 11.84YY14 pKa = 9.08PGTKK18 pKa = 8.74RR19 pKa = 11.84PSGKK23 pKa = 9.81RR24 pKa = 11.84LADD27 pKa = 3.53PRR29 pKa = 11.84DD30 pKa = 3.46PATRR34 pKa = 11.84DD35 pKa = 2.98ALTRR39 pKa = 11.84KK40 pKa = 10.23CPDD43 pKa = 3.49CNAEE47 pKa = 3.87PDD49 pKa = 3.58QWCVGIAEE57 pKa = 4.22NSRR60 pKa = 11.84TKK62 pKa = 10.52GRR64 pKa = 11.84RR65 pKa = 11.84ITRR68 pKa = 11.84LHH70 pKa = 6.42FSRR73 pKa = 11.84AKK75 pKa = 10.23FVEE78 pKa = 3.63TDD80 pKa = 3.29APVKK84 pKa = 10.67AGARR88 pKa = 3.65

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

14577

50

1338

227.8

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.418 ± 0.56

1.008 ± 0.184

7.093 ± 0.31

5.385 ± 0.445

2.964 ± 0.16

9.824 ± 0.635

1.983 ± 0.184

4.308 ± 0.176

3.403 ± 0.237

7.478 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.921 ± 0.102

2.765 ± 0.163

6.4 ± 0.338

3.094 ± 0.14

7.162 ± 0.493

4.727 ± 0.26

6.318 ± 0.309

6.668 ± 0.223

1.955 ± 0.14

2.127 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski