Flavobacterium phage vB_FspM_pippi8-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pippivirus; Flavobacterium virus Pippi

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LKS8|A0A6B9LKS8_9CAUD Virulence-associated protein E OS=Flavobacterium phage vB_FspM_pippi8-1 OX=2686244 GN=pippi81_gp020 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.57NKK4 pKa = 10.2DD5 pKa = 3.12LEE7 pKa = 4.61APFKK11 pKa = 11.32CEE13 pKa = 3.35ICLHH17 pKa = 6.0NFDD20 pKa = 3.77GCEE23 pKa = 3.6RR24 pKa = 11.84TEE26 pKa = 3.9NSEE29 pKa = 4.36ADD31 pKa = 3.27ICEE34 pKa = 3.68ACYY37 pKa = 10.72NEE39 pKa = 4.68EE40 pKa = 3.98EE41 pKa = 4.37NEE43 pKa = 4.02

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LKY1|A0A6B9LKY1_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspM_pippi8-1 OX=2686244 GN=pippi81_gp040 PE=4 SV=1
MM1 pKa = 7.72AFNFDD6 pKa = 3.59RR7 pKa = 11.84KK8 pKa = 10.06IKK10 pKa = 9.93EE11 pKa = 3.89FKK13 pKa = 9.81LAKK16 pKa = 9.24ATLPTIIGNIAKK28 pKa = 9.88RR29 pKa = 11.84HH30 pKa = 4.65FVQSFRR36 pKa = 11.84DD37 pKa = 3.64GGFTEE42 pKa = 5.69DD43 pKa = 6.09ALDD46 pKa = 3.41PWKK49 pKa = 10.74ARR51 pKa = 11.84KK52 pKa = 9.86RR53 pKa = 11.84GDD55 pKa = 3.14KK56 pKa = 10.33SDD58 pKa = 3.55RR59 pKa = 11.84NTRR62 pKa = 11.84TARR65 pKa = 11.84AILVKK70 pKa = 9.73TGHH73 pKa = 6.4LRR75 pKa = 11.84RR76 pKa = 11.84SIRR79 pKa = 11.84VRR81 pKa = 11.84VASFDD86 pKa = 3.8KK87 pKa = 11.06IEE89 pKa = 4.18IGAYY93 pKa = 8.35GVPYY97 pKa = 10.81AKK99 pKa = 10.29FHH101 pKa = 6.47NNGGGKK107 pKa = 9.61LPKK110 pKa = 9.83RR111 pKa = 11.84KK112 pKa = 9.69FIGHH116 pKa = 6.1SNALNKK122 pKa = 10.13KK123 pKa = 7.72IQSRR127 pKa = 11.84INKK130 pKa = 8.18EE131 pKa = 3.62IKK133 pKa = 10.42SILL136 pKa = 3.59

Molecular weight: 15.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11226

42

634

211.8

23.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.215 ± 0.491

0.909 ± 0.148

5.345 ± 0.216

8.115 ± 0.533

5.665 ± 0.357

6.066 ± 0.447

1.203 ± 0.121

7.777 ± 0.296

9.059 ± 0.539

8.293 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.746 ± 0.167

6.093 ± 0.31

2.859 ± 0.205

3.242 ± 0.146

3.59 ± 0.28

5.781 ± 0.319

6.182 ± 0.303

6.485 ± 0.256

0.935 ± 0.094

3.438 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski