Wallemia ichthyophaga (strain EXF-994 / CBS 113033)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Wallemiomycotina; Wallemiomycetes; Wallemiales; Wallemiaceae; Wallemia; Wallemia ichthyophaga

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9AKY9|R9AKY9_WALI9 Hydroxyacid-oxoacid transhydrogenase OS=Wallemia ichthyophaga (strain EXF-994 / CBS 113033) OX=1299270 GN=J056_003424 PE=3 SV=1
MM1 pKa = 7.3VLLKK5 pKa = 9.84TATTLLAATAAYY17 pKa = 9.34AWNHH21 pKa = 5.09TASDD25 pKa = 4.22VIATGTMGTTNPPEE39 pKa = 3.92ATMGTEE45 pKa = 4.46LNQTSVARR53 pKa = 11.84LVSINSIDD61 pKa = 3.76DD62 pKa = 3.49FCFFGPKK69 pKa = 8.57EE70 pKa = 4.15ANVTIGDD77 pKa = 3.93TEE79 pKa = 4.47GEE81 pKa = 4.17QVAWCTKK88 pKa = 8.17PRR90 pKa = 11.84NNARR94 pKa = 11.84VIPDD98 pKa = 3.27GTITSTHH105 pKa = 5.23FVKK108 pKa = 10.2TPAYY112 pKa = 8.72VQVSGFGDD120 pKa = 3.53LTKK123 pKa = 10.41IGVKK127 pKa = 10.51DD128 pKa = 3.4GDD130 pKa = 3.85YY131 pKa = 11.16GGEE134 pKa = 4.16LDD136 pKa = 3.7PHH138 pKa = 6.59GATGDD143 pKa = 3.82GNPVGGNVTSDD154 pKa = 2.95IVNGTSTSYY163 pKa = 11.27AEE165 pKa = 3.67WMEE168 pKa = 4.58FISYY172 pKa = 9.35EE173 pKa = 3.97QFCIRR178 pKa = 11.84ICTASNDD185 pKa = 3.28TWGAPVMCEE194 pKa = 3.73HH195 pKa = 7.14KK196 pKa = 10.3LDD198 pKa = 3.69EE199 pKa = 4.55MGCGFVMPDD208 pKa = 3.64AGDD211 pKa = 3.39SDD213 pKa = 4.49VFEE216 pKa = 4.6TCDD219 pKa = 3.95ADD221 pKa = 3.74VAYY224 pKa = 9.41PPGVYY229 pKa = 9.95PIDD232 pKa = 3.88GSYY235 pKa = 9.18STFAQRR241 pKa = 11.84YY242 pKa = 4.82TGSYY246 pKa = 10.64SSGTTGAMVGYY257 pKa = 7.88TVGVTDD263 pKa = 4.48TPTAAQMTPSSSNCVTQSSLAGNNVDD289 pKa = 3.67VSATGVIEE297 pKa = 4.95DD298 pKa = 4.72NRR300 pKa = 11.84DD301 pKa = 3.36DD302 pKa = 5.91DD303 pKa = 4.89SDD305 pKa = 4.76SNSDD309 pKa = 3.6PDD311 pKa = 4.11SNKK314 pKa = 9.89SDD316 pKa = 3.38KK317 pKa = 11.34SNPDD321 pKa = 3.24GNDD324 pKa = 3.36GGSTGSGQTSANAGDD339 pKa = 4.41GDD341 pKa = 4.17SSATTFTGFATVSAIALAVLAIALL365 pKa = 4.06

Molecular weight: 37.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9AFR4|R9AFR4_WALI9 Uncharacterized protein OS=Wallemia ichthyophaga (strain EXF-994 / CBS 113033) OX=1299270 GN=J056_004385 PE=4 SV=1
MM1 pKa = 7.87PGNIPLFPTFTTLLNRR17 pKa = 11.84RR18 pKa = 11.84PTLPAISAIAASGLRR33 pKa = 11.84FGSRR37 pKa = 11.84GTDD40 pKa = 3.46YY41 pKa = 11.15QPSTRR46 pKa = 11.84KK47 pKa = 9.59RR48 pKa = 11.84KK49 pKa = 8.51RR50 pKa = 11.84TFGFLARR57 pKa = 11.84IRR59 pKa = 11.84SRR61 pKa = 11.84TGRR64 pKa = 11.84KK65 pKa = 8.96IISRR69 pKa = 11.84RR70 pKa = 11.84LKK72 pKa = 10.29KK73 pKa = 10.25GKK75 pKa = 10.45KK76 pKa = 9.46NMSHH80 pKa = 6.78

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4836

0

4836

2342023

32

4950

484.3

53.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.508 ± 0.032

1.044 ± 0.013

6.212 ± 0.028

6.147 ± 0.033

3.605 ± 0.021

5.893 ± 0.031

2.745 ± 0.02

5.357 ± 0.026

5.839 ± 0.037

9.144 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.012

4.804 ± 0.023

5.167 ± 0.033

4.513 ± 0.03

5.4 ± 0.026

8.983 ± 0.042

5.745 ± 0.017

5.902 ± 0.03

1.091 ± 0.011

2.835 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski