Buceros rhinoceros silvestris

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091HC09|A0A091HC09_BUCRH ADGRG1 C-terminal fragment (Fragment) OS=Buceros rhinoceros silvestris OX=175836 GN=N320_04747 PE=4 SV=1
MM1 pKa = 7.1FVGYY5 pKa = 10.38EE6 pKa = 3.97DD7 pKa = 5.31DD8 pKa = 5.56DD9 pKa = 3.95NAEE12 pKa = 4.12AYY14 pKa = 9.88EE15 pKa = 4.3DD16 pKa = 3.88EE17 pKa = 5.28LYY19 pKa = 10.91HH20 pKa = 6.86EE21 pKa = 4.9EE22 pKa = 4.17TSSEE26 pKa = 3.9QSVDD30 pKa = 3.66SEE32 pKa = 5.19VEE34 pKa = 3.64FHH36 pKa = 7.02LYY38 pKa = 9.8SQIHH42 pKa = 5.37YY43 pKa = 8.93SQNLGGISTLEE54 pKa = 3.99MDD56 pKa = 4.68EE57 pKa = 4.69EE58 pKa = 4.58MDD60 pKa = 5.87DD61 pKa = 3.96EE62 pKa = 4.44MDD64 pKa = 4.13DD65 pKa = 4.21AGVTVLAEE73 pKa = 4.1KK74 pKa = 9.6WCEE77 pKa = 3.85DD78 pKa = 3.22KK79 pKa = 11.1EE80 pKa = 4.01IAEE83 pKa = 5.07FIDD86 pKa = 4.31CGSQVSEE93 pKa = 4.61VIVLSDD99 pKa = 3.64TPDD102 pKa = 3.13EE103 pKa = 4.55DD104 pKa = 4.33SVYY107 pKa = 10.72KK108 pKa = 10.8SKK110 pKa = 11.2AKK112 pKa = 10.14KK113 pKa = 10.05SKK115 pKa = 10.82SSLAQDD121 pKa = 4.85AIQVHH126 pKa = 6.6RR127 pKa = 11.84GSSTPNHH134 pKa = 5.07TTAAGCHH141 pKa = 5.04TLCPAGSGNLSPVSSAGVHH160 pKa = 5.76AVQDD164 pKa = 3.61VLVIDD169 pKa = 4.9DD170 pKa = 4.04SSEE173 pKa = 4.08EE174 pKa = 3.85EE175 pKa = 4.38SLVSEE180 pKa = 4.37SDD182 pKa = 3.73HH183 pKa = 7.18VEE185 pKa = 3.33SWMLLGANADD195 pKa = 4.28DD196 pKa = 4.16RR197 pKa = 11.84DD198 pKa = 3.81EE199 pKa = 7.33DD200 pKa = 4.03IMLNLEE206 pKa = 4.2GCATPVSQGG215 pKa = 3.0

Molecular weight: 23.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091I4G9|A0A091I4G9_BUCRH Indian hedgehog protein (Fragment) OS=Buceros rhinoceros silvestris OX=175836 GN=N320_09342 PE=4 SV=1
MM1 pKa = 7.67AAQSAPKK8 pKa = 10.16VVLKK12 pKa = 10.04STTKK16 pKa = 9.35MSLNEE21 pKa = 4.25RR22 pKa = 11.84FTNMLKK28 pKa = 10.36NKK30 pKa = 9.73QPMPVNIRR38 pKa = 11.84APMQQQQQLASARR51 pKa = 11.84NRR53 pKa = 11.84RR54 pKa = 11.84LAQQMEE60 pKa = 4.32NRR62 pKa = 11.84PSVQAALKK70 pKa = 10.37LKK72 pKa = 10.39QKK74 pKa = 9.42SLKK77 pKa = 9.72QRR79 pKa = 11.84LGKK82 pKa = 10.77SNIQARR88 pKa = 11.84LGRR91 pKa = 11.84PAGLLRR97 pKa = 11.84GGRR100 pKa = 11.84GLSPRR105 pKa = 11.84MGLRR109 pKa = 11.84RR110 pKa = 11.84GGIRR114 pKa = 11.84GRR116 pKa = 11.84GGPGRR121 pKa = 11.84GGLGRR126 pKa = 11.84GAMGRR131 pKa = 11.84GGLGGRR137 pKa = 11.84GEE139 pKa = 4.33CRR141 pKa = 11.84AAARR145 pKa = 4.11

Molecular weight: 15.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10603

0

10603

4535817

31

5001

427.8

48.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.419 ± 0.021

2.225 ± 0.023

5.065 ± 0.016

7.224 ± 0.029

3.896 ± 0.017

5.82 ± 0.03

2.526 ± 0.013

4.943 ± 0.019

6.433 ± 0.029

9.715 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.011

4.148 ± 0.016

5.279 ± 0.028

4.691 ± 0.023

5.21 ± 0.02

8.27 ± 0.032

5.416 ± 0.018

6.334 ± 0.018

1.184 ± 0.009

2.973 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski