Paenibacillus lycopersici
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5671 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C0FU95|A0A6C0FU95_9BACL TCR/Tet family MFS transporter OS=Paenibacillus lycopersici OX=2704462 GN=GXP70_10995 PE=4 SV=1
MM1 pKa = 7.33 PAEE4 pKa = 3.99 TAEE7 pKa = 4.07 AVAWALQQSIVVDD20 pKa = 3.9 GDD22 pKa = 3.67 AYY24 pKa = 9.92 PVKK27 pKa = 10.53 KK28 pKa = 9.45 DD29 pKa = 3.36 TCQMLVDD36 pKa = 4.13 KK37 pKa = 11.16 VSEE40 pKa = 4.11 YY41 pKa = 11.08 FNANNIEE48 pKa = 4.06 YY49 pKa = 10.45 GSFSFADD56 pKa = 3.74 LMAGGFLDD64 pKa = 3.77
Molecular weight: 6.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.846
IPC_protein 3.719
Toseland 3.528
ProMoST 3.795
Dawson 3.719
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A6C0FX16|A0A6C0FX16_9BACL ATP-binding cassette domain-containing protein OS=Paenibacillus lycopersici OX=2704462 GN=GXP70_17945 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.82 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.48 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.2 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 8.65 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.98 VLSAA44 pKa = 4.11
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5671
0
5671
1865584
25
2985
329.0
36.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.164 ± 0.048
0.794 ± 0.011
5.397 ± 0.027
6.305 ± 0.038
4.005 ± 0.024
8.048 ± 0.029
2.098 ± 0.016
6.006 ± 0.029
4.546 ± 0.028
9.916 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.754 ± 0.017
3.466 ± 0.024
4.296 ± 0.02
3.614 ± 0.021
5.853 ± 0.037
5.924 ± 0.029
5.226 ± 0.036
6.931 ± 0.029
1.321 ± 0.014
3.335 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here