Soybean chlorotic mottle virus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P15633|Y3_SOCMV Uncharacterized protein 3 OS=Soybean chlorotic mottle virus OX=10651 GN=ORF III PE=4 SV=2
MM1 pKa = 7.82 PGDD4 pKa = 3.19 NWTNSIIFRR13 pKa = 11.84 IKK15 pKa = 8.81 TLKK18 pKa = 10.55 DD19 pKa = 3.56 QIIEE23 pKa = 3.92 WSKK26 pKa = 11.31 NPTEE30 pKa = 4.0 EE31 pKa = 4.3 NEE33 pKa = 4.3 YY34 pKa = 10.62 FSEE37 pKa = 5.09 KK38 pKa = 10.43 YY39 pKa = 10.21 LEE41 pKa = 4.8 KK42 pKa = 10.66 INTLTKK48 pKa = 10.13 TLDD51 pKa = 3.04 WCEE54 pKa = 4.02 KK55 pKa = 8.66 SNKK58 pKa = 9.82 SEE60 pKa = 4.75 VEE62 pKa = 4.07 LRR64 pKa = 11.84 WLNQALSLPHH74 pKa = 5.86 QVPPPRR80 pKa = 11.84 YY81 pKa = 8.21 SYY83 pKa = 10.65 IRR85 pKa = 11.84 SEE87 pKa = 4.01 SSRR90 pKa = 11.84 NNLRR94 pKa = 11.84 NSARR98 pKa = 11.84 NQPQNLVSEE107 pKa = 4.33 QDD109 pKa = 3.16 SDD111 pKa = 4.55 SNRR114 pKa = 11.84 EE115 pKa = 3.76 NN116 pKa = 3.28
Molecular weight: 13.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.636
IPC2_protein 5.83
IPC_protein 5.753
Toseland 5.855
ProMoST 5.919
Dawson 5.83
Bjellqvist 5.881
Wikipedia 5.728
Rodwell 5.779
Grimsley 5.868
Solomon 5.83
Lehninger 5.792
Nozaki 5.982
DTASelect 6.122
Thurlkill 5.995
EMBOSS 5.944
Sillero 6.097
Patrickios 4.495
IPC_peptide 5.842
IPC2_peptide 6.059
IPC2.peptide.svr19 6.281
Protein with the highest isoelectric point:
>sp|P15635|Y8_SOCMV Uncharacterized protein 8 OS=Soybean chlorotic mottle virus OX=10651 GN=ORF VIII PE=4 SV=1
MM1 pKa = 8.09 DD2 pKa = 4.08 VGNQILEE9 pKa = 4.28 PKK11 pKa = 9.28 NLKK14 pKa = 10.25 RR15 pKa = 11.84 NSGKK19 pKa = 10.0 LRR21 pKa = 11.84 NILKK25 pKa = 10.12 RR26 pKa = 11.84 ISGNGIIKK34 pKa = 9.46 EE35 pKa = 4.12 RR36 pKa = 11.84 KK37 pKa = 8.7 RR38 pKa = 11.84 RR39 pKa = 11.84 LGKK42 pKa = 9.73 NAPLEE47 pKa = 3.98 NSKK50 pKa = 10.52 LVQQVKK56 pKa = 10.24 RR57 pKa = 11.84 NVNVGYY63 pKa = 8.41 ATKK66 pKa = 10.26 KK67 pKa = 9.27 DD68 pKa = 3.75 TMRR71 pKa = 11.84 MSAQKK76 pKa = 9.53 RR77 pKa = 11.84 TTKK80 pKa = 10.37 RR81 pKa = 11.84 LKK83 pKa = 10.02 PP84 pKa = 3.61
Molecular weight: 9.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.176
IPC2_protein 10.262
IPC_protein 11.52
Toseland 11.754
ProMoST 12.149
Dawson 11.769
Bjellqvist 11.696
Wikipedia 12.179
Rodwell 11.886
Grimsley 11.813
Solomon 12.193
Lehninger 12.106
Nozaki 11.754
DTASelect 11.696
Thurlkill 11.754
EMBOSS 12.237
Sillero 11.754
Patrickios 11.608
IPC_peptide 12.193
IPC2_peptide 11.125
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2639
84
692
293.2
34.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.486 ± 0.524
1.667 ± 0.371
4.509 ± 0.221
9.284 ± 0.53
4.168 ± 0.343
3.676 ± 0.341
2.008 ± 0.178
7.73 ± 0.69
11.216 ± 0.401
10.421 ± 0.606
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.288 ± 0.201
6.366 ± 0.665
3.751 ± 0.324
5.343 ± 0.384
4.358 ± 0.385
7.275 ± 0.383
5.116 ± 0.218
3.827 ± 0.342
1.061 ± 0.237
3.448 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here