Amino acid dipepetide frequency for Mycoplasma agalactiae (strain PG2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.58AlaAla: 3.58 ± 0.158
0.316AlaCys: 0.316 ± 0.035
2.729AlaAsp: 2.729 ± 0.108
3.56AlaGlu: 3.56 ± 0.156
2.932AlaPhe: 2.932 ± 0.122
2.761AlaGly: 2.761 ± 0.119
0.879AlaHis: 0.879 ± 0.056
5.541AlaIle: 5.541 ± 0.179
6.78AlaLys: 6.78 ± 0.201
5.933AlaLeu: 5.933 ± 0.168
1.179AlaMet: 1.179 ± 0.081
4.347AlaAsn: 4.347 ± 0.148
1.454AlaPro: 1.454 ± 0.073
1.786AlaGln: 1.786 ± 0.089
2.042AlaArg: 2.042 ± 0.102
4.211AlaSer: 4.211 ± 0.138
3.04AlaThr: 3.04 ± 0.12
3.176AlaVal: 3.176 ± 0.125
0.487AlaTrp: 0.487 ± 0.052
2.341AlaTyr: 2.341 ± 0.104
0.0AlaXaa: 0.0 ± 0.0
Cys
0.396CysAla: 0.396 ± 0.045
0.056CysCys: 0.056 ± 0.016
0.328CysAsp: 0.328 ± 0.036
0.384CysGlu: 0.384 ± 0.047
0.38CysPhe: 0.38 ± 0.037
0.404CysGly: 0.404 ± 0.044
0.156CysHis: 0.156 ± 0.026
0.396CysIle: 0.396 ± 0.043
0.396CysLys: 0.396 ± 0.04
0.563CysLeu: 0.563 ± 0.048
0.084CysMet: 0.084 ± 0.021
0.408CysAsn: 0.408 ± 0.042
0.2CysPro: 0.2 ± 0.029
0.168CysGln: 0.168 ± 0.027
0.156CysArg: 0.156 ± 0.023
0.376CysSer: 0.376 ± 0.038
0.26CysThr: 0.26 ± 0.029
0.276CysVal: 0.276 ± 0.034
0.056CysTrp: 0.056 ± 0.013
0.2CysTyr: 0.2 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.084AspAla: 3.084 ± 0.126
0.236AspCys: 0.236 ± 0.032
3.208AspAsp: 3.208 ± 0.137
5.022AspGlu: 5.022 ± 0.17
3.42AspPhe: 3.42 ± 0.141
2.669AspGly: 2.669 ± 0.113
0.675AspHis: 0.675 ± 0.051
4.874AspIle: 4.874 ± 0.165
6.468AspLys: 6.468 ± 0.184
5.417AspLeu: 5.417 ± 0.153
0.799AspMet: 0.799 ± 0.06
3.935AspAsn: 3.935 ± 0.136
1.662AspPro: 1.662 ± 0.081
1.366AspGln: 1.366 ± 0.073
1.494AspArg: 1.494 ± 0.084
4.179AspSer: 4.179 ± 0.13
2.217AspThr: 2.217 ± 0.088
3.18AspVal: 3.18 ± 0.132
0.527AspTrp: 0.527 ± 0.04
2.681AspTyr: 2.681 ± 0.124
0.0AspXaa: 0.0 ± 0.0
Glu
3.979GluAla: 3.979 ± 0.138
0.292GluCys: 0.292 ± 0.032
2.817GluAsp: 2.817 ± 0.125
4.463GluGlu: 4.463 ± 0.193
3.811GluPhe: 3.811 ± 0.133
2.573GluGly: 2.573 ± 0.117
1.107GluHis: 1.107 ± 0.059
6.424GluIle: 6.424 ± 0.165
7.519GluLys: 7.519 ± 0.197
7.407GluLeu: 7.407 ± 0.199
1.338GluMet: 1.338 ± 0.074
5.613GluAsn: 5.613 ± 0.153
1.438GluPro: 1.438 ± 0.096
2.257GluGln: 2.257 ± 0.107
1.902GluArg: 1.902 ± 0.107
4.351GluSer: 4.351 ± 0.135
2.976GluThr: 2.976 ± 0.121
3.847GluVal: 3.847 ± 0.137
0.631GluTrp: 0.631 ± 0.056
2.964GluTyr: 2.964 ± 0.122
0.0GluXaa: 0.0 ± 0.0
Phe
3.376PheAla: 3.376 ± 0.129
0.324PheCys: 0.324 ± 0.04
3.676PheAsp: 3.676 ± 0.123
3.372PheGlu: 3.372 ± 0.121
2.369PhePhe: 2.369 ± 0.113
2.817PheGly: 2.817 ± 0.112
0.687PheHis: 0.687 ± 0.056
4.742PheIle: 4.742 ± 0.163
5.222PheLys: 5.222 ± 0.159
4.411PheLeu: 4.411 ± 0.146
1.003PheMet: 1.003 ± 0.07
4.043PheAsn: 4.043 ± 0.13
1.163PhePro: 1.163 ± 0.062
1.123PheGln: 1.123 ± 0.071
1.482PheArg: 1.482 ± 0.078
4.075PheSer: 4.075 ± 0.143
2.625PheThr: 2.625 ± 0.109
3.216PheVal: 3.216 ± 0.13
0.531PheTrp: 0.531 ± 0.052
2.165PheTyr: 2.165 ± 0.109
0.0PheXaa: 0.0 ± 0.0
Gly
3.14GlyAla: 3.14 ± 0.122
0.324GlyCys: 0.324 ± 0.04
2.441GlyAsp: 2.441 ± 0.099
2.637GlyGlu: 2.637 ± 0.116
2.621GlyPhe: 2.621 ± 0.129
2.885GlyGly: 2.885 ± 0.138
0.867GlyHis: 0.867 ± 0.067
4.662GlyIle: 4.662 ± 0.17
4.702GlyLys: 4.702 ± 0.165
4.63GlyLeu: 4.63 ± 0.188
0.867GlyMet: 0.867 ± 0.058
2.589GlyAsn: 2.589 ± 0.116
0.843GlyPro: 0.843 ± 0.068
1.622GlyGln: 1.622 ± 0.083
1.478GlyArg: 1.478 ± 0.087
3.324GlySer: 3.324 ± 0.116
2.972GlyThr: 2.972 ± 0.13
2.753GlyVal: 2.753 ± 0.114
0.451GlyTrp: 0.451 ± 0.046
2.078GlyTyr: 2.078 ± 0.101
0.0GlyXaa: 0.0 ± 0.0
His
0.767HisAla: 0.767 ± 0.06
0.092HisCys: 0.092 ± 0.024
0.731HisAsp: 0.731 ± 0.06
0.911HisGlu: 0.911 ± 0.063
0.827HisPhe: 0.827 ± 0.057
0.883HisGly: 0.883 ± 0.064
0.276HisHis: 0.276 ± 0.035
1.37HisIle: 1.37 ± 0.077
1.518HisLys: 1.518 ± 0.091
1.426HisLeu: 1.426 ± 0.08
0.264HisMet: 0.264 ± 0.033
1.239HisAsn: 1.239 ± 0.066
0.567HisPro: 0.567 ± 0.055
0.431HisGln: 0.431 ± 0.039
0.499HisArg: 0.499 ± 0.046
1.139HisSer: 1.139 ± 0.073
0.775HisThr: 0.775 ± 0.069
0.623HisVal: 0.623 ± 0.054
0.136HisTrp: 0.136 ± 0.026
0.675HisTyr: 0.675 ± 0.052
0.0HisXaa: 0.0 ± 0.0
Ile
5.421IleAla: 5.421 ± 0.161
0.619IleCys: 0.619 ± 0.046
5.653IleAsp: 5.653 ± 0.153
5.765IleGlu: 5.765 ± 0.177
4.239IlePhe: 4.239 ± 0.173
4.227IleGly: 4.227 ± 0.144
1.211IleHis: 1.211 ± 0.074
7.419IleIle: 7.419 ± 0.223
8.837IleLys: 8.837 ± 0.218
7.595IleLeu: 7.595 ± 0.202
1.474IleMet: 1.474 ± 0.078
7.167IleAsn: 7.167 ± 0.205
2.661IlePro: 2.661 ± 0.106
2.018IleGln: 2.018 ± 0.105
2.393IleArg: 2.393 ± 0.099
7.012IleSer: 7.012 ± 0.17
4.511IleThr: 4.511 ± 0.132
5.278IleVal: 5.278 ± 0.167
0.775IleTrp: 0.775 ± 0.064
3.524IleTyr: 3.524 ± 0.129
0.0IleXaa: 0.0 ± 0.0
Lys
5.689LysAla: 5.689 ± 0.184
0.583LysCys: 0.583 ± 0.056
6.54LysAsp: 6.54 ± 0.24
7.922LysGlu: 7.922 ± 0.215
4.926LysPhe: 4.926 ± 0.176
3.899LysGly: 3.899 ± 0.138
1.742LysHis: 1.742 ± 0.088
9.021LysIle: 9.021 ± 0.173
9.656LysLys: 9.656 ± 0.258
9.569LysLeu: 9.569 ± 0.225
2.477LysMet: 2.477 ± 0.103
8.406LysAsn: 8.406 ± 0.214
2.625LysPro: 2.625 ± 0.111
3.224LysGln: 3.224 ± 0.122
2.996LysArg: 2.996 ± 0.107
6.864LysSer: 6.864 ± 0.159
5.206LysThr: 5.206 ± 0.15
6.101LysVal: 6.101 ± 0.178
1.139LysTrp: 1.139 ± 0.066
4.678LysTyr: 4.678 ± 0.155
0.0LysXaa: 0.0 ± 0.0
Leu
5.825LeuAla: 5.825 ± 0.156
0.619LeuCys: 0.619 ± 0.046
5.493LeuAsp: 5.493 ± 0.156
6.32LeuGlu: 6.32 ± 0.212
5.07LeuPhe: 5.07 ± 0.171
4.63LeuGly: 4.63 ± 0.148
1.306LeuHis: 1.306 ± 0.081
8.334LeuIle: 8.334 ± 0.214
9.241LeuLys: 9.241 ± 0.227
8.901LeuLeu: 8.901 ± 0.253
1.846LeuMet: 1.846 ± 0.077
6.484LeuAsn: 6.484 ± 0.183
2.972LeuPro: 2.972 ± 0.126
2.245LeuGln: 2.245 ± 0.097
2.853LeuArg: 2.853 ± 0.114
7.327LeuSer: 7.327 ± 0.19
4.491LeuThr: 4.491 ± 0.126
6.169LeuVal: 6.169 ± 0.191
0.835LeuTrp: 0.835 ± 0.052
2.98LeuTyr: 2.98 ± 0.12
0.0LeuXaa: 0.0 ± 0.0
Met
1.147MetAla: 1.147 ± 0.084
0.136MetCys: 0.136 ± 0.021
0.887MetAsp: 0.887 ± 0.062
0.875MetGlu: 0.875 ± 0.064
1.211MetPhe: 1.211 ± 0.081
0.779MetGly: 0.779 ± 0.059
0.435MetHis: 0.435 ± 0.041
1.558MetIle: 1.558 ± 0.068
1.762MetLys: 1.762 ± 0.078
1.826MetLeu: 1.826 ± 0.083
0.34MetMet: 0.34 ± 0.033
1.458MetAsn: 1.458 ± 0.076
0.927MetPro: 0.927 ± 0.062
0.763MetGln: 0.763 ± 0.053
0.547MetArg: 0.547 ± 0.049
1.514MetSer: 1.514 ± 0.075
0.899MetThr: 0.899 ± 0.055
0.851MetVal: 0.851 ± 0.063
0.152MetTrp: 0.152 ± 0.028
0.587MetTyr: 0.587 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
3.684AsnAla: 3.684 ± 0.126
0.292AsnCys: 0.292 ± 0.035
4.523AsnAsp: 4.523 ± 0.143
5.501AsnGlu: 5.501 ± 0.167
3.528AsnPhe: 3.528 ± 0.128
3.46AsnGly: 3.46 ± 0.127
1.035AsnHis: 1.035 ± 0.058
6.364AsnIle: 6.364 ± 0.147
8.714AsnLys: 8.714 ± 0.211
6.36AsnLeu: 6.36 ± 0.206
1.274AsnMet: 1.274 ± 0.079
6.217AsnAsn: 6.217 ± 0.217
2.493AsnPro: 2.493 ± 0.093
2.105AsnGln: 2.105 ± 0.107
2.082AsnArg: 2.082 ± 0.085
5.697AsnSer: 5.697 ± 0.203
3.0AsnThr: 3.0 ± 0.109
4.335AsnVal: 4.335 ± 0.149
0.759AsnTrp: 0.759 ± 0.068
3.436AsnTyr: 3.436 ± 0.119
0.0AsnXaa: 0.0 ± 0.0
Pro
1.622ProAla: 1.622 ± 0.088
0.124ProCys: 0.124 ± 0.021
1.51ProAsp: 1.51 ± 0.08
2.249ProGlu: 2.249 ± 0.111
1.486ProPhe: 1.486 ± 0.078
1.558ProGly: 1.558 ± 0.083
0.459ProHis: 0.459 ± 0.044
2.389ProIle: 2.389 ± 0.1
2.589ProLys: 2.589 ± 0.101
2.421ProLeu: 2.421 ± 0.1
0.451ProMet: 0.451 ± 0.044
1.978ProAsn: 1.978 ± 0.102
0.539ProPro: 0.539 ± 0.046
0.675ProGln: 0.675 ± 0.059
0.719ProArg: 0.719 ± 0.061
2.07ProSer: 2.07 ± 0.099
1.678ProThr: 1.678 ± 0.067
1.77ProVal: 1.77 ± 0.098
0.284ProTrp: 0.284 ± 0.03
1.262ProTyr: 1.262 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
1.638GlnAla: 1.638 ± 0.077
0.112GlnCys: 0.112 ± 0.021
1.354GlnAsp: 1.354 ± 0.086
1.662GlnGlu: 1.662 ± 0.1
1.482GlnPhe: 1.482 ± 0.085
1.334GlnGly: 1.334 ± 0.086
0.4GlnHis: 0.4 ± 0.042
2.585GlnIle: 2.585 ± 0.097
3.24GlnLys: 3.24 ± 0.132
2.609GlnLeu: 2.609 ± 0.094
0.579GlnMet: 0.579 ± 0.043
2.369GlnAsn: 2.369 ± 0.118
0.751GlnPro: 0.751 ± 0.054
0.787GlnGln: 0.787 ± 0.061
0.935GlnArg: 0.935 ± 0.071
1.774GlnSer: 1.774 ± 0.089
1.314GlnThr: 1.314 ± 0.079
1.474GlnVal: 1.474 ± 0.086
0.252GlnTrp: 0.252 ± 0.034
1.127GlnTyr: 1.127 ± 0.07
0.0GlnXaa: 0.0 ± 0.0
Arg
1.882ArgAla: 1.882 ± 0.083
0.164ArgCys: 0.164 ± 0.027
1.646ArgAsp: 1.646 ± 0.087
1.978ArgGlu: 1.978 ± 0.082
1.534ArgPhe: 1.534 ± 0.079
1.318ArgGly: 1.318 ± 0.081
0.495ArgHis: 0.495 ± 0.038
2.693ArgIle: 2.693 ± 0.11
2.877ArgLys: 2.877 ± 0.123
2.681ArgLeu: 2.681 ± 0.132
0.795ArgMet: 0.795 ± 0.052
1.946ArgAsn: 1.946 ± 0.102
0.923ArgPro: 0.923 ± 0.069
1.039ArgGln: 1.039 ± 0.072
1.151ArgArg: 1.151 ± 0.076
1.906ArgSer: 1.906 ± 0.099
1.286ArgThr: 1.286 ± 0.085
1.95ArgVal: 1.95 ± 0.083
0.292ArgTrp: 0.292 ± 0.031
1.294ArgTyr: 1.294 ± 0.069
0.0ArgXaa: 0.0 ± 0.0
Ser
4.419SerAla: 4.419 ± 0.132
0.491SerCys: 0.491 ± 0.045
4.147SerAsp: 4.147 ± 0.129
4.642SerGlu: 4.642 ± 0.167
4.111SerPhe: 4.111 ± 0.137
3.608SerGly: 3.608 ± 0.129
1.067SerHis: 1.067 ± 0.078
6.013SerIle: 6.013 ± 0.176
7.875SerLys: 7.875 ± 0.174
7.211SerLeu: 7.211 ± 0.24
1.215SerMet: 1.215 ± 0.077
5.545SerAsn: 5.545 ± 0.173
1.778SerPro: 1.778 ± 0.082
2.125SerGln: 2.125 ± 0.085
2.197SerArg: 2.197 ± 0.109
5.661SerSer: 5.661 ± 0.173
3.264SerThr: 3.264 ± 0.135
4.167SerVal: 4.167 ± 0.127
0.735SerTrp: 0.735 ± 0.057
2.956SerTyr: 2.956 ± 0.12
0.0SerXaa: 0.0 ± 0.0
Thr
2.377ThrAla: 2.377 ± 0.094
0.236ThrCys: 0.236 ± 0.034
2.681ThrAsp: 2.681 ± 0.108
2.733ThrGlu: 2.733 ± 0.137
2.693ThrPhe: 2.693 ± 0.11
2.869ThrGly: 2.869 ± 0.105
0.783ThrHis: 0.783 ± 0.061
4.287ThrIle: 4.287 ± 0.124
5.042ThrLys: 5.042 ± 0.154
4.606ThrLeu: 4.606 ± 0.136
0.803ThrMet: 0.803 ± 0.061
3.624ThrAsn: 3.624 ± 0.135
1.666ThrPro: 1.666 ± 0.101
1.115ThrGln: 1.115 ± 0.077
1.398ThrArg: 1.398 ± 0.069
3.48ThrSer: 3.48 ± 0.114
2.561ThrThr: 2.561 ± 0.108
2.889ThrVal: 2.889 ± 0.111
0.38ThrTrp: 0.38 ± 0.039
2.034ThrTyr: 2.034 ± 0.088
0.0ThrXaa: 0.0 ± 0.0
Val
3.979ValAla: 3.979 ± 0.144
0.336ValCys: 0.336 ± 0.038
3.384ValAsp: 3.384 ± 0.125
3.943ValGlu: 3.943 ± 0.126
2.932ValPhe: 2.932 ± 0.131
2.924ValGly: 2.924 ± 0.143
0.859ValHis: 0.859 ± 0.061
5.114ValIle: 5.114 ± 0.174
5.761ValLys: 5.761 ± 0.163
5.581ValLeu: 5.581 ± 0.145
1.035ValMet: 1.035 ± 0.074
3.923ValAsn: 3.923 ± 0.108
1.802ValPro: 1.802 ± 0.083
1.546ValGln: 1.546 ± 0.074
1.698ValArg: 1.698 ± 0.095
4.535ValSer: 4.535 ± 0.14
2.773ValThr: 2.773 ± 0.123
3.564ValVal: 3.564 ± 0.154
0.563ValTrp: 0.563 ± 0.048
2.225ValTyr: 2.225 ± 0.103
0.0ValXaa: 0.0 ± 0.0
Trp
0.543TrpAla: 0.543 ± 0.048
0.068TrpCys: 0.068 ± 0.016
0.523TrpAsp: 0.523 ± 0.047
0.667TrpGlu: 0.667 ± 0.046
0.551TrpPhe: 0.551 ± 0.049
0.4TrpGly: 0.4 ± 0.045
0.148TrpHis: 0.148 ± 0.03
0.855TrpIle: 0.855 ± 0.073
0.759TrpLys: 0.759 ± 0.065
0.983TrpLeu: 0.983 ± 0.066
0.188TrpMet: 0.188 ± 0.026
0.819TrpAsn: 0.819 ± 0.064
0.268TrpPro: 0.268 ± 0.033
0.256TrpGln: 0.256 ± 0.036
0.324TrpArg: 0.324 ± 0.036
0.551TrpSer: 0.551 ± 0.051
0.591TrpThr: 0.591 ± 0.055
0.607TrpVal: 0.607 ± 0.057
0.124TrpTrp: 0.124 ± 0.023
0.4TrpTyr: 0.4 ± 0.045
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.525TyrAla: 2.525 ± 0.1
0.22TyrCys: 0.22 ± 0.029
2.845TyrAsp: 2.845 ± 0.125
3.108TyrGlu: 3.108 ± 0.129
2.297TyrPhe: 2.297 ± 0.094
1.89TyrGly: 1.89 ± 0.097
0.563TyrHis: 0.563 ± 0.051
3.156TyrIle: 3.156 ± 0.115
4.307TyrLys: 4.307 ± 0.152
3.811TyrLeu: 3.811 ± 0.151
0.639TyrMet: 0.639 ± 0.052
2.749TyrAsn: 2.749 ± 0.107
1.103TyrPro: 1.103 ± 0.064
1.127TyrGln: 1.127 ± 0.079
1.502TyrArg: 1.502 ± 0.084
3.184TyrSer: 3.184 ± 0.121
1.814TyrThr: 1.814 ± 0.091
2.253TyrVal: 2.253 ± 0.087
0.515TyrTrp: 0.515 ± 0.048
1.834TyrTyr: 1.834 ± 0.106
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 726 proteins (250301 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski