Candidatus Nitrosarchaeum limnium BG20

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrosopumilales; Nitrosopumilaceae; Nitrosarchaeum; Candidatus Nitrosarchaeum limnium

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S2E115|S2E115_9ARCH Monoamine oxidase OS=Candidatus Nitrosarchaeum limnium BG20 OX=859192 GN=BG20_I1566 PE=4 SV=1
MM1 pKa = 6.62TTYY4 pKa = 10.57KK5 pKa = 10.89APDD8 pKa = 3.67ADD10 pKa = 3.95GDD12 pKa = 4.36GIPDD16 pKa = 4.08VKK18 pKa = 10.6DD19 pKa = 3.27ACPNEE24 pKa = 3.95PEE26 pKa = 4.37NFNGYY31 pKa = 9.9LDD33 pKa = 3.82WDD35 pKa = 4.3GCPDD39 pKa = 3.45TPGAQSTAPTRR50 pKa = 11.84PDD52 pKa = 3.19SDD54 pKa = 3.75KK55 pKa = 11.65DD56 pKa = 4.02GIPDD60 pKa = 5.45DD61 pKa = 5.21VDD63 pKa = 3.39QCPSNPEE70 pKa = 3.42TWNKK74 pKa = 9.72YY75 pKa = 9.9RR76 pKa = 11.84DD77 pKa = 3.63TDD79 pKa = 3.78GCPDD83 pKa = 3.47ITPEE87 pKa = 3.88QQRR90 pKa = 11.84FVHH93 pKa = 7.3DD94 pKa = 4.89DD95 pKa = 3.7DD96 pKa = 6.66LDD98 pKa = 4.92GIINDD103 pKa = 3.39VDD105 pKa = 3.54KK106 pKa = 11.39CPLVPEE112 pKa = 4.81DD113 pKa = 4.22YY114 pKa = 10.72IGIIDD119 pKa = 4.73GCPEE123 pKa = 3.78SS124 pKa = 3.86

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S2E673|S2E673_9ARCH Uncharacterized protein OS=Candidatus Nitrosarchaeum limnium BG20 OX=859192 GN=BG20_I0880 PE=4 SV=1
MM1 pKa = 7.19ATSKK5 pKa = 10.87AKK7 pKa = 10.18RR8 pKa = 11.84VEE10 pKa = 4.06AAKK13 pKa = 10.31KK14 pKa = 9.45AARR17 pKa = 11.84TRR19 pKa = 11.84KK20 pKa = 9.73RR21 pKa = 11.84NAALKK26 pKa = 9.35AAEE29 pKa = 4.45AARR32 pKa = 11.84MAARR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.52RR39 pKa = 11.84SRR41 pKa = 11.84AAKK44 pKa = 9.6RR45 pKa = 11.84AAATRR50 pKa = 11.84KK51 pKa = 9.24KK52 pKa = 10.23AAPRR56 pKa = 11.84RR57 pKa = 11.84KK58 pKa = 9.58APKK61 pKa = 9.42RR62 pKa = 11.84KK63 pKa = 8.39AAKK66 pKa = 9.79RR67 pKa = 11.84RR68 pKa = 11.84TASKK72 pKa = 10.01RR73 pKa = 11.84AAPKK77 pKa = 10.0RR78 pKa = 11.84KK79 pKa = 8.46AAKK82 pKa = 9.79RR83 pKa = 11.84RR84 pKa = 11.84TASKK88 pKa = 10.01RR89 pKa = 11.84AAPKK93 pKa = 10.0RR94 pKa = 11.84KK95 pKa = 8.41AAKK98 pKa = 9.71RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 3.63

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

547141

30

1263

208.8

23.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.706 ± 0.049

1.056 ± 0.023

5.663 ± 0.046

6.572 ± 0.055

4.46 ± 0.044

6.312 ± 0.058

1.8 ± 0.023

9.471 ± 0.065

8.919 ± 0.068

8.62 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.031

5.336 ± 0.05

3.688 ± 0.039

3.172 ± 0.032

3.395 ± 0.041

7.309 ± 0.054

5.629 ± 0.047

6.297 ± 0.043

0.892 ± 0.016

3.14 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski