Clostridium sp. CAG:245

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6BIF4|R6BIF4_9CLOT Stage II sporulation protein D OS=Clostridium sp. CAG:245 OX=1262784 GN=BN559_00238 PE=4 SV=1
MM1 pKa = 7.71AEE3 pKa = 3.9FSEE6 pKa = 4.99KK7 pKa = 9.84YY8 pKa = 10.42SKK10 pKa = 10.31IIKK13 pKa = 9.96DD14 pKa = 3.44IEE16 pKa = 4.06KK17 pKa = 10.58SISDD21 pKa = 3.41EE22 pKa = 4.1KK23 pKa = 10.91EE24 pKa = 3.75RR25 pKa = 11.84EE26 pKa = 3.93IVLKK30 pKa = 10.51KK31 pKa = 10.56VSEE34 pKa = 4.09LSFMYY39 pKa = 9.67MDD41 pKa = 4.27VIDD44 pKa = 5.41RR45 pKa = 11.84ISNVNSEE52 pKa = 4.15RR53 pKa = 11.84MDD55 pKa = 5.38DD56 pKa = 3.89IEE58 pKa = 4.24KK59 pKa = 10.37HH60 pKa = 5.14QDD62 pKa = 3.18KK63 pKa = 10.84LDD65 pKa = 3.74EE66 pKa = 5.26KK67 pKa = 10.41IGKK70 pKa = 9.35VKK72 pKa = 10.06DD73 pKa = 3.55TVDD76 pKa = 4.81LIKK79 pKa = 10.57KK80 pKa = 10.07DD81 pKa = 3.17IYY83 pKa = 10.94EE84 pKa = 4.53DD85 pKa = 3.49EE86 pKa = 5.86DD87 pKa = 4.53YY88 pKa = 11.29DD89 pKa = 4.64FEE91 pKa = 5.23IVCPYY96 pKa = 10.22CNHH99 pKa = 6.31EE100 pKa = 4.41FVADD104 pKa = 3.77IEE106 pKa = 5.03DD107 pKa = 3.71EE108 pKa = 4.16LKK110 pKa = 11.11EE111 pKa = 4.19EE112 pKa = 4.33IEE114 pKa = 5.46CPEE117 pKa = 4.07CHH119 pKa = 6.89NKK121 pKa = 9.37IEE123 pKa = 4.61LDD125 pKa = 3.2WDD127 pKa = 4.08GEE129 pKa = 4.44DD130 pKa = 5.76DD131 pKa = 3.96DD132 pKa = 5.27CCSGGCCGCGHH143 pKa = 7.09HH144 pKa = 7.4DD145 pKa = 3.9EE146 pKa = 5.2CGCDD150 pKa = 3.59EE151 pKa = 5.17DD152 pKa = 6.21EE153 pKa = 5.31EE154 pKa = 6.48DD155 pKa = 4.54SDD157 pKa = 4.36TDD159 pKa = 4.75NEE161 pKa = 4.27EE162 pKa = 4.98KK163 pKa = 10.66DD164 pKa = 5.42DD165 pKa = 5.16NDD167 pKa = 5.15DD168 pKa = 4.79DD169 pKa = 3.99MM170 pKa = 7.81

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6B2B0|R6B2B0_9CLOT 30S ribosomal protein S10 OS=Clostridium sp. CAG:245 OX=1262784 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84LAGVDD8 pKa = 3.65LPRR11 pKa = 11.84EE12 pKa = 3.87KK13 pKa = 10.22RR14 pKa = 11.84IEE16 pKa = 3.89IGLTYY21 pKa = 9.99IYY23 pKa = 10.58GIGLARR29 pKa = 11.84SQEE32 pKa = 4.0ILKK35 pKa = 10.51KK36 pKa = 10.79AGINPDD42 pKa = 3.06IRR44 pKa = 11.84VKK46 pKa = 10.9DD47 pKa = 3.64LTDD50 pKa = 3.35EE51 pKa = 4.06QVQLIRR57 pKa = 11.84NAMEE61 pKa = 5.24GYY63 pKa = 8.39TLEE66 pKa = 4.68GDD68 pKa = 3.9LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.55VALNIKK78 pKa = 9.97RR79 pKa = 11.84LTEE82 pKa = 3.73IGCYY86 pKa = 9.46RR87 pKa = 11.84GTRR90 pKa = 11.84HH91 pKa = 6.52RR92 pKa = 11.84RR93 pKa = 11.84GLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.44GPRR113 pKa = 11.84KK114 pKa = 9.45LVSKK118 pKa = 10.34SKK120 pKa = 10.63KK121 pKa = 9.7

Molecular weight: 13.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1330

0

1330

403135

29

2816

303.1

34.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.621 ± 0.061

1.092 ± 0.025

5.605 ± 0.053

8.156 ± 0.079

3.855 ± 0.056

5.738 ± 0.069

1.224 ± 0.021

9.675 ± 0.093

9.62 ± 0.072

8.187 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.03

6.872 ± 0.077

2.589 ± 0.04

2.835 ± 0.032

3.407 ± 0.052

5.798 ± 0.057

5.705 ± 0.071

6.422 ± 0.063

0.626 ± 0.019

4.422 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski