Streptomyces sp. MP131-18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2RL99|A0A1V2RL99_9ACTN Uncharacterized protein OS=Streptomyces sp. MP131-18 OX=1857892 GN=STBA_39260 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84TTGRR6 pKa = 11.84IALAVLTATTLLLTACSSNDD26 pKa = 3.44RR27 pKa = 11.84PDD29 pKa = 3.35EE30 pKa = 4.33RR31 pKa = 11.84SPSDD35 pKa = 3.72PEE37 pKa = 4.26SDD39 pKa = 3.51ASSPAPDD46 pKa = 3.75APALEE51 pKa = 4.61PLPEE55 pKa = 4.71EE56 pKa = 4.72IPAEE60 pKa = 3.88LQPYY64 pKa = 8.77YY65 pKa = 10.16EE66 pKa = 5.44QEE68 pKa = 4.4LTWSSCGAVGFQCATLAVPLDD89 pKa = 4.0YY90 pKa = 11.51EE91 pKa = 4.47NVDD94 pKa = 3.6PAQDD98 pKa = 3.18IQLTVTRR105 pKa = 11.84ARR107 pKa = 11.84ATEE110 pKa = 3.78SGEE113 pKa = 4.17RR114 pKa = 11.84IGALLMNPGGPGASAVDD131 pKa = 4.38FAQDD135 pKa = 2.94AATYY139 pKa = 9.0LYY141 pKa = 9.62PPEE144 pKa = 3.75VRR146 pKa = 11.84ARR148 pKa = 11.84YY149 pKa = 10.6DD150 pKa = 3.33MVGLDD155 pKa = 4.25ARR157 pKa = 11.84GTGQSEE163 pKa = 4.08PVEE166 pKa = 4.59CLTGDD171 pKa = 3.91AMDD174 pKa = 6.05DD175 pKa = 3.56YY176 pKa = 10.72TLTDD180 pKa = 3.7RR181 pKa = 11.84TPDD184 pKa = 2.86SDD186 pKa = 4.85AEE188 pKa = 4.12VDD190 pKa = 3.94EE191 pKa = 5.47LLAAYY196 pKa = 10.3DD197 pKa = 5.37DD198 pKa = 4.41FAQGCQEE205 pKa = 3.71RR206 pKa = 11.84SGALLEE212 pKa = 4.95HH213 pKa = 7.06ISTIEE218 pKa = 3.9SARR221 pKa = 11.84DD222 pKa = 3.37MDD224 pKa = 4.29VLRR227 pKa = 11.84AALGDD232 pKa = 3.83EE233 pKa = 3.99KK234 pKa = 11.06LHH236 pKa = 5.82YY237 pKa = 10.88VGFSYY242 pKa = 8.92GTKK245 pKa = 10.42LGATYY250 pKa = 10.81AGLFPQRR257 pKa = 11.84TGRR260 pKa = 11.84LVLDD264 pKa = 3.95AAMDD268 pKa = 4.08PRR270 pKa = 11.84LSTLDD275 pKa = 3.35TDD277 pKa = 4.37RR278 pKa = 11.84EE279 pKa = 4.1QAGGFEE285 pKa = 4.07TAFRR289 pKa = 11.84SFAEE293 pKa = 4.38DD294 pKa = 3.26CTPRR298 pKa = 11.84DD299 pKa = 3.45SCALGNDD306 pKa = 4.17GADD309 pKa = 3.7GASQALLDD317 pKa = 4.49FFAEE321 pKa = 4.05VDD323 pKa = 3.92AEE325 pKa = 4.09PLPTGDD331 pKa = 3.71EE332 pKa = 4.09EE333 pKa = 5.36RR334 pKa = 11.84PLTEE338 pKa = 4.23SLASTGVSYY347 pKa = 11.45ALYY350 pKa = 10.8AEE352 pKa = 5.14DD353 pKa = 4.28LWPMLRR359 pKa = 11.84DD360 pKa = 3.64ALATAIEE367 pKa = 4.94DD368 pKa = 3.94GDD370 pKa = 4.13GSALLDD376 pKa = 4.1LADD379 pKa = 4.61AYY381 pKa = 11.05NEE383 pKa = 4.08RR384 pKa = 11.84EE385 pKa = 4.12ANGTYY390 pKa = 8.32GTTMFAFPAISCLDD404 pKa = 3.61SPAGNEE410 pKa = 3.87DD411 pKa = 3.31ADD413 pKa = 3.99AVRR416 pKa = 11.84SALAAYY422 pKa = 9.37EE423 pKa = 4.11EE424 pKa = 4.49ASPTFGRR431 pKa = 11.84DD432 pKa = 3.42FAWATLLCAAWPVDD446 pKa = 3.61PSGAPVSIPAADD458 pKa = 3.45ATDD461 pKa = 3.22ILVIGTLRR469 pKa = 11.84DD470 pKa = 3.29PATPYY475 pKa = 10.98AWAEE479 pKa = 3.9GLADD483 pKa = 3.68QLEE486 pKa = 4.49TGILLTYY493 pKa = 10.73DD494 pKa = 3.68GDD496 pKa = 3.74GHH498 pKa = 6.1GAYY501 pKa = 9.86GGASEE506 pKa = 5.15CVDD509 pKa = 3.56SAVNDD514 pKa = 3.69YY515 pKa = 11.17LLEE518 pKa = 4.39NVSPEE523 pKa = 4.96DD524 pKa = 3.5GTTCC528 pKa = 4.71

Molecular weight: 55.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2RK93|A0A1V2RK93_9ACTN Phosphotransferase enzyme family protein OS=Streptomyces sp. MP131-18 OX=1857892 GN=STBA_36440 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.81GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7037

0

7037

2289126

29

4553

325.3

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.292 ± 0.054

0.799 ± 0.008

6.014 ± 0.024

5.936 ± 0.025

2.689 ± 0.016

9.761 ± 0.036

2.407 ± 0.016

3.022 ± 0.021

1.396 ± 0.016

10.43 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.683 ± 0.012

1.672 ± 0.016

6.369 ± 0.03

2.659 ± 0.02

8.638 ± 0.036

4.695 ± 0.025

5.883 ± 0.027

8.138 ± 0.028

1.569 ± 0.012

1.946 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski