Corynebacterium spheniscorum
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2RJ62|A0A1I2RJ62_9CORY Uncharacterized protein (Fragment) OS=Corynebacterium spheniscorum OX=185761 GN=SAMN05660282_00824 PE=4 SV=1
MM1 pKa = 7.61 SSLPNDD7 pKa = 3.33 YY8 pKa = 9.92 TYY10 pKa = 11.3 SEE12 pKa = 3.98 DD13 pKa = 4.35 HH14 pKa = 6.12 EE15 pKa = 5.05 WINATAEE22 pKa = 3.97 DD23 pKa = 3.86 AAGKK27 pKa = 9.42 IVRR30 pKa = 11.84 VGITSVATEE39 pKa = 3.86 RR40 pKa = 11.84 LGEE43 pKa = 4.23 VVFAEE48 pKa = 4.38 LPEE51 pKa = 4.62 VGDD54 pKa = 4.09 SVTHH58 pKa = 6.58 SEE60 pKa = 4.18 TCGEE64 pKa = 4.22 VEE66 pKa = 4.29 STKK69 pKa = 10.7 SVSDD73 pKa = 4.02 LYY75 pKa = 11.42 SPVSGTVTAVNDD87 pKa = 4.18 AVHH90 pKa = 7.42 DD91 pKa = 4.65 DD92 pKa = 3.8 YY93 pKa = 12.12 SVINSDD99 pKa = 3.59 PFGEE103 pKa = 4.41 GWLFEE108 pKa = 4.45 VEE110 pKa = 4.08 VTEE113 pKa = 5.12 AGEE116 pKa = 4.0 LMTAEE121 pKa = 5.12 EE122 pKa = 4.3 YY123 pKa = 11.23 AKK125 pKa = 11.18 ANGVDD130 pKa = 3.32
Molecular weight: 13.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.63
ProMoST 3.897
Dawson 3.77
Bjellqvist 3.973
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.668
EMBOSS 3.681
Sillero 3.923
Patrickios 1.85
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A1I2UKA4|A0A1I2UKA4_9CORY Ring-1 2-phenylacetyl-CoA epoxidase subunit PaaA OS=Corynebacterium spheniscorum OX=185761 GN=SAMN05660282_01913 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.88 GFRR22 pKa = 11.84 IRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AVVAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.7 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2153
0
2153
709375
27
3039
329.5
35.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.434 ± 0.059
0.845 ± 0.018
5.685 ± 0.046
6.685 ± 0.059
3.23 ± 0.034
8.177 ± 0.053
2.355 ± 0.026
5.346 ± 0.043
3.469 ± 0.044
9.946 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.293 ± 0.024
2.786 ± 0.031
5.387 ± 0.046
3.325 ± 0.028
6.217 ± 0.055
5.736 ± 0.033
5.756 ± 0.042
7.744 ± 0.046
1.364 ± 0.023
2.221 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here