Cronartium ribicola mitovirus 2
Average proteome isoelectric point is 8.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KCN6|A0A191KCN6_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 2 OX=1816485 GN=RdRp PE=4 SV=1
MM1 pKa = 7.66 NIQAMMRR8 pKa = 11.84 WLAVHH13 pKa = 6.54 HH14 pKa = 6.4 SCSPEE19 pKa = 3.63 LMNVVEE25 pKa = 4.44 TVLIKK30 pKa = 10.01 WDD32 pKa = 3.66 LLVRR36 pKa = 11.84 TKK38 pKa = 10.83 GIKK41 pKa = 8.79 EE42 pKa = 3.97 TVKK45 pKa = 10.28 YY46 pKa = 9.66 FKK48 pKa = 10.56 ASRR51 pKa = 11.84 LHH53 pKa = 5.01 CTRR56 pKa = 11.84 YY57 pKa = 9.74 ICGDD61 pKa = 3.69 PLLVSEE67 pKa = 5.87 GISISKK73 pKa = 10.56 DD74 pKa = 3.14 GLPNLIKK81 pKa = 10.83 GHH83 pKa = 5.79 GKK85 pKa = 9.91 SFFRR89 pKa = 11.84 NKK91 pKa = 9.86 KK92 pKa = 8.38 INEE95 pKa = 3.45 IRR97 pKa = 11.84 IILTILSMGRR107 pKa = 11.84 IIPGTGEE114 pKa = 4.13 CDD116 pKa = 3.09 LTPISKK122 pKa = 9.27 PWLGQIPTSITRR134 pKa = 11.84 WINSRR139 pKa = 11.84 TDD141 pKa = 2.87 ISKK144 pKa = 10.66 VEE146 pKa = 4.09 IEE148 pKa = 4.08 WKK150 pKa = 9.46 EE151 pKa = 3.89 PHH153 pKa = 6.85 WSTKK157 pKa = 10.46 AGPKK161 pKa = 9.15 GQAIASSIEE170 pKa = 4.02 DD171 pKa = 3.66 LASLPEE177 pKa = 3.98 TLRR180 pKa = 11.84 TDD182 pKa = 2.46 IRR184 pKa = 11.84 TIGGEE189 pKa = 4.06 EE190 pKa = 3.75 LSEE193 pKa = 4.39 YY194 pKa = 10.34 IDD196 pKa = 3.66 TLEE199 pKa = 4.5 PYY201 pKa = 10.08 YY202 pKa = 11.12 KK203 pKa = 10.66 NILPNPKK210 pKa = 9.35 RR211 pKa = 11.84 RR212 pKa = 11.84 SKK214 pKa = 10.34 LASSIRR220 pKa = 11.84 RR221 pKa = 11.84 LHH223 pKa = 5.95 IVLDD227 pKa = 3.81 KK228 pKa = 10.6 EE229 pKa = 4.49 YY230 pKa = 8.72 KK231 pKa = 8.37 TRR233 pKa = 11.84 PIAIIDD239 pKa = 3.63 YY240 pKa = 8.68 WSQTSLVPYY249 pKa = 9.62 HH250 pKa = 7.23 DD251 pKa = 5.73 YY252 pKa = 10.44 IMKK255 pKa = 9.58 WIQKK259 pKa = 9.93 QYY261 pKa = 11.54 GDD263 pKa = 3.64 YY264 pKa = 10.11 TLQSKK269 pKa = 9.89 VGKK272 pKa = 10.31 QIVKK276 pKa = 9.15 GQGRR280 pKa = 11.84 IYY282 pKa = 11.01 SFDD285 pKa = 3.6 LQNATDD291 pKa = 4.16 RR292 pKa = 11.84 FPLEE296 pKa = 3.5 FQKK299 pKa = 10.72 IVFAKK304 pKa = 10.32 FFGSKK309 pKa = 10.15 KK310 pKa = 8.73 ADD312 pKa = 2.63 AWARR316 pKa = 11.84 IITQYY321 pKa = 10.9 PFEE324 pKa = 5.2 LKK326 pKa = 10.61 DD327 pKa = 3.67 GSTVSYY333 pKa = 11.27 NCGQPIGAYY342 pKa = 9.7 SSWAIFTASHH352 pKa = 6.39 HH353 pKa = 7.09 LILQYY358 pKa = 10.55 IRR360 pKa = 11.84 FIHH363 pKa = 6.96 PEE365 pKa = 3.93 TIYY368 pKa = 11.0 GVLGDD373 pKa = 4.35 DD374 pKa = 3.6 VVIRR378 pKa = 11.84 GDD380 pKa = 3.3 RR381 pKa = 11.84 GAFLYY386 pKa = 10.63 KK387 pKa = 10.44 EE388 pKa = 4.36 VMSCLDD394 pKa = 3.48 VKK396 pKa = 10.74 ISEE399 pKa = 4.65 SKK401 pKa = 8.33 THH403 pKa = 5.58 VSNDD407 pKa = 2.86 TFEE410 pKa = 4.1 FAKK413 pKa = 10.35 RR414 pKa = 11.84 WFHH417 pKa = 6.74 KK418 pKa = 10.35 GAEE421 pKa = 4.13 ITPFPLNEE429 pKa = 4.82 AIEE432 pKa = 4.63 AGTDD436 pKa = 3.35 VAKK439 pKa = 10.8 ASQLFLNLPLRR450 pKa = 11.84 GWYY453 pKa = 10.29 LGGNDD458 pKa = 3.99 LSQSILEE465 pKa = 4.12 FMKK468 pKa = 10.94 SKK470 pKa = 10.33 IVPSNFASYY479 pKa = 10.36 LARR482 pKa = 11.84 RR483 pKa = 11.84 SARR486 pKa = 11.84 FIYY489 pKa = 8.42 FQKK492 pKa = 10.18 WAITGCYY499 pKa = 9.74 SYY501 pKa = 11.2 LSKK504 pKa = 10.84 LHH506 pKa = 6.37 PLVLVSGSSVCNHH519 pKa = 5.5 TEE521 pKa = 3.9 RR522 pKa = 11.84 LRR524 pKa = 11.84 TAIMLGISKK533 pKa = 8.6 ATAKK537 pKa = 10.31 EE538 pKa = 3.65 ISQILEE544 pKa = 4.81 DD545 pKa = 4.5 INTLLKK551 pKa = 10.73 RR552 pKa = 11.84 KK553 pKa = 9.73 DD554 pKa = 3.24 IQGLVDD560 pKa = 3.48 QTTDD564 pKa = 3.11 SPSYY568 pKa = 9.12 TDD570 pKa = 4.2 LRR572 pKa = 11.84 PGEE575 pKa = 4.1 YY576 pKa = 10.51 ALGKK580 pKa = 10.26 ISLKK584 pKa = 10.03 LQKK587 pKa = 9.77 EE588 pKa = 4.12 LRR590 pKa = 11.84 QFDD593 pKa = 3.78 VDD595 pKa = 2.89 IHH597 pKa = 6.03 MLSKK601 pKa = 10.13 MAAVPDD607 pKa = 3.97 WATHH611 pKa = 5.7 LGEE614 pKa = 4.78 ASISKK619 pKa = 10.47 LKK621 pKa = 10.33 GIPVGRR627 pKa = 11.84 RR628 pKa = 11.84 PDD630 pKa = 3.76 PDD632 pKa = 3.54 TYY634 pKa = 10.23 ISEE637 pKa = 4.17 RR638 pKa = 11.84 RR639 pKa = 11.84 AVTATRR645 pKa = 11.84 SLLRR649 pKa = 11.84 VYY651 pKa = 11.35 NNFNTEE657 pKa = 3.95 TQQFFAIRR665 pKa = 11.84 PAIHH669 pKa = 6.9 RR670 pKa = 11.84 PDD672 pKa = 3.51 ANKK675 pKa = 10.61 SIGSHH680 pKa = 4.96 VRR682 pKa = 11.84 TAAQAISS689 pKa = 3.26
Molecular weight: 78.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.827
IPC2_protein 8.829
IPC_protein 8.712
Toseland 9.428
ProMoST 9.224
Dawson 9.721
Bjellqvist 9.443
Wikipedia 9.897
Rodwell 9.999
Grimsley 9.794
Solomon 9.736
Lehninger 9.692
Nozaki 9.531
DTASelect 9.399
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.663
Patrickios 4.927
IPC_peptide 9.736
IPC2_peptide 8.039
IPC2.peptide.svr19 7.831
Protein with the highest isoelectric point:
>tr|A0A191KCN6|A0A191KCN6_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 2 OX=1816485 GN=RdRp PE=4 SV=1
MM1 pKa = 7.66 NIQAMMRR8 pKa = 11.84 WLAVHH13 pKa = 6.54 HH14 pKa = 6.4 SCSPEE19 pKa = 3.63 LMNVVEE25 pKa = 4.44 TVLIKK30 pKa = 10.01 WDD32 pKa = 3.66 LLVRR36 pKa = 11.84 TKK38 pKa = 10.83 GIKK41 pKa = 8.79 EE42 pKa = 3.97 TVKK45 pKa = 10.28 YY46 pKa = 9.66 FKK48 pKa = 10.56 ASRR51 pKa = 11.84 LHH53 pKa = 5.01 CTRR56 pKa = 11.84 YY57 pKa = 9.74 ICGDD61 pKa = 3.69 PLLVSEE67 pKa = 5.87 GISISKK73 pKa = 10.56 DD74 pKa = 3.14 GLPNLIKK81 pKa = 10.83 GHH83 pKa = 5.79 GKK85 pKa = 9.91 SFFRR89 pKa = 11.84 NKK91 pKa = 9.86 KK92 pKa = 8.38 INEE95 pKa = 3.45 IRR97 pKa = 11.84 IILTILSMGRR107 pKa = 11.84 IIPGTGEE114 pKa = 4.13 CDD116 pKa = 3.09 LTPISKK122 pKa = 9.27 PWLGQIPTSITRR134 pKa = 11.84 WINSRR139 pKa = 11.84 TDD141 pKa = 2.87 ISKK144 pKa = 10.66 VEE146 pKa = 4.09 IEE148 pKa = 4.08 WKK150 pKa = 9.46 EE151 pKa = 3.89 PHH153 pKa = 6.85 WSTKK157 pKa = 10.46 AGPKK161 pKa = 9.15 GQAIASSIEE170 pKa = 4.02 DD171 pKa = 3.66 LASLPEE177 pKa = 3.98 TLRR180 pKa = 11.84 TDD182 pKa = 2.46 IRR184 pKa = 11.84 TIGGEE189 pKa = 4.06 EE190 pKa = 3.75 LSEE193 pKa = 4.39 YY194 pKa = 10.34 IDD196 pKa = 3.66 TLEE199 pKa = 4.5 PYY201 pKa = 10.08 YY202 pKa = 11.12 KK203 pKa = 10.66 NILPNPKK210 pKa = 9.35 RR211 pKa = 11.84 RR212 pKa = 11.84 SKK214 pKa = 10.34 LASSIRR220 pKa = 11.84 RR221 pKa = 11.84 LHH223 pKa = 5.95 IVLDD227 pKa = 3.81 KK228 pKa = 10.6 EE229 pKa = 4.49 YY230 pKa = 8.72 KK231 pKa = 8.37 TRR233 pKa = 11.84 PIAIIDD239 pKa = 3.63 YY240 pKa = 8.68 WSQTSLVPYY249 pKa = 9.62 HH250 pKa = 7.23 DD251 pKa = 5.73 YY252 pKa = 10.44 IMKK255 pKa = 9.58 WIQKK259 pKa = 9.93 QYY261 pKa = 11.54 GDD263 pKa = 3.64 YY264 pKa = 10.11 TLQSKK269 pKa = 9.89 VGKK272 pKa = 10.31 QIVKK276 pKa = 9.15 GQGRR280 pKa = 11.84 IYY282 pKa = 11.01 SFDD285 pKa = 3.6 LQNATDD291 pKa = 4.16 RR292 pKa = 11.84 FPLEE296 pKa = 3.5 FQKK299 pKa = 10.72 IVFAKK304 pKa = 10.32 FFGSKK309 pKa = 10.15 KK310 pKa = 8.73 ADD312 pKa = 2.63 AWARR316 pKa = 11.84 IITQYY321 pKa = 10.9 PFEE324 pKa = 5.2 LKK326 pKa = 10.61 DD327 pKa = 3.67 GSTVSYY333 pKa = 11.27 NCGQPIGAYY342 pKa = 9.7 SSWAIFTASHH352 pKa = 6.39 HH353 pKa = 7.09 LILQYY358 pKa = 10.55 IRR360 pKa = 11.84 FIHH363 pKa = 6.96 PEE365 pKa = 3.93 TIYY368 pKa = 11.0 GVLGDD373 pKa = 4.35 DD374 pKa = 3.6 VVIRR378 pKa = 11.84 GDD380 pKa = 3.3 RR381 pKa = 11.84 GAFLYY386 pKa = 10.63 KK387 pKa = 10.44 EE388 pKa = 4.36 VMSCLDD394 pKa = 3.48 VKK396 pKa = 10.74 ISEE399 pKa = 4.65 SKK401 pKa = 8.33 THH403 pKa = 5.58 VSNDD407 pKa = 2.86 TFEE410 pKa = 4.1 FAKK413 pKa = 10.35 RR414 pKa = 11.84 WFHH417 pKa = 6.74 KK418 pKa = 10.35 GAEE421 pKa = 4.13 ITPFPLNEE429 pKa = 4.82 AIEE432 pKa = 4.63 AGTDD436 pKa = 3.35 VAKK439 pKa = 10.8 ASQLFLNLPLRR450 pKa = 11.84 GWYY453 pKa = 10.29 LGGNDD458 pKa = 3.99 LSQSILEE465 pKa = 4.12 FMKK468 pKa = 10.94 SKK470 pKa = 10.33 IVPSNFASYY479 pKa = 10.36 LARR482 pKa = 11.84 RR483 pKa = 11.84 SARR486 pKa = 11.84 FIYY489 pKa = 8.42 FQKK492 pKa = 10.18 WAITGCYY499 pKa = 9.74 SYY501 pKa = 11.2 LSKK504 pKa = 10.84 LHH506 pKa = 6.37 PLVLVSGSSVCNHH519 pKa = 5.5 TEE521 pKa = 3.9 RR522 pKa = 11.84 LRR524 pKa = 11.84 TAIMLGISKK533 pKa = 8.6 ATAKK537 pKa = 10.31 EE538 pKa = 3.65 ISQILEE544 pKa = 4.81 DD545 pKa = 4.5 INTLLKK551 pKa = 10.73 RR552 pKa = 11.84 KK553 pKa = 9.73 DD554 pKa = 3.24 IQGLVDD560 pKa = 3.48 QTTDD564 pKa = 3.11 SPSYY568 pKa = 9.12 TDD570 pKa = 4.2 LRR572 pKa = 11.84 PGEE575 pKa = 4.1 YY576 pKa = 10.51 ALGKK580 pKa = 10.26 ISLKK584 pKa = 10.03 LQKK587 pKa = 9.77 EE588 pKa = 4.12 LRR590 pKa = 11.84 QFDD593 pKa = 3.78 VDD595 pKa = 2.89 IHH597 pKa = 6.03 MLSKK601 pKa = 10.13 MAAVPDD607 pKa = 3.97 WATHH611 pKa = 5.7 LGEE614 pKa = 4.78 ASISKK619 pKa = 10.47 LKK621 pKa = 10.33 GIPVGRR627 pKa = 11.84 RR628 pKa = 11.84 PDD630 pKa = 3.76 PDD632 pKa = 3.54 TYY634 pKa = 10.23 ISEE637 pKa = 4.17 RR638 pKa = 11.84 RR639 pKa = 11.84 AVTATRR645 pKa = 11.84 SLLRR649 pKa = 11.84 VYY651 pKa = 11.35 NNFNTEE657 pKa = 3.95 TQQFFAIRR665 pKa = 11.84 PAIHH669 pKa = 6.9 RR670 pKa = 11.84 PDD672 pKa = 3.51 ANKK675 pKa = 10.61 SIGSHH680 pKa = 4.96 VRR682 pKa = 11.84 TAAQAISS689 pKa = 3.26
Molecular weight: 78.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.827
IPC2_protein 8.829
IPC_protein 8.712
Toseland 9.428
ProMoST 9.224
Dawson 9.721
Bjellqvist 9.443
Wikipedia 9.897
Rodwell 9.999
Grimsley 9.794
Solomon 9.736
Lehninger 9.692
Nozaki 9.531
DTASelect 9.399
Thurlkill 9.56
EMBOSS 9.867
Sillero 9.663
Patrickios 4.927
IPC_peptide 9.736
IPC2_peptide 8.039
IPC2.peptide.svr19 7.831
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
689
689
689
689.0
78.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.386 ± 0.0
1.161 ± 0.0
4.935 ± 0.0
4.935 ± 0.0
3.774 ± 0.0
5.951 ± 0.0
2.612 ± 0.0
9.724 ± 0.0
7.402 ± 0.0
8.999 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.597 ± 0.0
3.048 ± 0.0
4.644 ± 0.0
3.628 ± 0.0
5.951 ± 0.0
8.563 ± 0.0
6.096 ± 0.0
4.644 ± 0.0
2.032 ± 0.0
3.919 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here