Yam virus X
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096XMC6|A0A096XMC6_9VIRU Movement protein TGBp3 OS=Yam virus X OX=1503864 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.5 QPLDD5 pKa = 3.63 WLVALLLLLGTVFAAITIYY24 pKa = 10.62 KK25 pKa = 9.41 EE26 pKa = 4.31 PPCTLVLDD34 pKa = 4.82 GASLTITGCKK44 pKa = 8.33 LTPEE48 pKa = 5.61 LISQISGLSPLRR60 pKa = 11.84 GLSLQEE66 pKa = 3.95 FF67 pKa = 4.37
Molecular weight: 7.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.572
IPC2_protein 4.813
IPC_protein 4.406
Toseland 4.253
ProMoST 4.533
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.253
Rodwell 4.24
Grimsley 4.177
Solomon 4.329
Lehninger 4.279
Nozaki 4.495
DTASelect 4.584
Thurlkill 4.291
EMBOSS 4.266
Sillero 4.507
Patrickios 3.503
IPC_peptide 4.342
IPC2_peptide 4.495
IPC2.peptide.svr19 4.484
Protein with the highest isoelectric point:
>tr|A0A096XLM0|A0A096XLM0_9VIRU Triple gene block 1 OS=Yam virus X OX=1503864 GN=TGB1 PE=4 SV=1
MM1 pKa = 7.95 PLTPPPDD8 pKa = 3.36 HH9 pKa = 6.38 STTFRR14 pKa = 11.84 IAAATVGLALIFFTLTRR31 pKa = 11.84 STLPHH36 pKa = 6.48 VGDD39 pKa = 4.85 NIHH42 pKa = 6.59 HH43 pKa = 6.84 LPHH46 pKa = 6.61 GGCYY50 pKa = 9.29 RR51 pKa = 11.84 DD52 pKa = 3.46 GTKK55 pKa = 9.77 TIKK58 pKa = 10.76 YY59 pKa = 8.97 NSPSANANNTPAWALPAVLILSALIYY85 pKa = 8.85 ATSRR89 pKa = 11.84 LASCSVTSTRR99 pKa = 11.84 YY100 pKa = 9.91 CIRR103 pKa = 11.84 SDD105 pKa = 3.49 NNLL108 pKa = 3.22
Molecular weight: 11.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.798
IPC2_protein 8.595
IPC_protein 8.829
Toseland 8.756
ProMoST 8.887
Dawson 9.136
Bjellqvist 9.297
Wikipedia 9.238
Rodwell 9.151
Grimsley 8.756
Solomon 9.414
Lehninger 9.385
Nozaki 9.385
DTASelect 9.107
Thurlkill 9.107
EMBOSS 9.282
Sillero 9.37
Patrickios 4.024
IPC_peptide 9.385
IPC2_peptide 8.726
IPC2.peptide.svr19 8.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1997
67
1386
399.4
44.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.112 ± 1.376
1.953 ± 0.246
5.058 ± 0.451
5.859 ± 1.286
5.508 ± 0.724
4.407 ± 0.671
2.554 ± 0.503
6.209 ± 0.232
5.809 ± 1.399
10.516 ± 1.745
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.953 ± 0.476
5.258 ± 0.632
5.158 ± 0.754
3.906 ± 0.588
4.457 ± 0.367
7.011 ± 0.546
7.461 ± 1.131
5.108 ± 0.711
1.002 ± 0.183
2.704 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here