Candidatus Kryptobacter tengchongensis

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Kryptonia; Candidatus Kryptobacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A656D9N8|A0A656D9N8_9BACT Uncharacterized protein (Fragment) OS=Candidatus Kryptobacter tengchongensis OX=1643429 GN=JGI24_01116 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 4.87VDD4 pKa = 5.82RR5 pKa = 11.84NMLWTPEE12 pKa = 4.48LIRR15 pKa = 11.84YY16 pKa = 9.4LEE18 pKa = 4.42GAPWPATKK26 pKa = 10.25QEE28 pKa = 4.4LIEE31 pKa = 3.87YY32 pKa = 8.79AKK34 pKa = 10.61RR35 pKa = 11.84IGAPPEE41 pKa = 4.1VIQNLEE47 pKa = 3.98EE48 pKa = 5.71LEE50 pKa = 4.95DD51 pKa = 4.12SDD53 pKa = 5.09EE54 pKa = 4.32EE55 pKa = 4.66YY56 pKa = 11.39NGIEE60 pKa = 5.1DD61 pKa = 3.4IWPDD65 pKa = 3.56YY66 pKa = 11.16SPEE69 pKa = 4.65DD70 pKa = 3.89EE71 pKa = 5.58DD72 pKa = 5.55DD73 pKa = 3.76YY74 pKa = 11.78LYY76 pKa = 11.42GEE78 pKa = 4.35EE79 pKa = 5.4DD80 pKa = 3.71EE81 pKa = 4.72YY82 pKa = 12.02

Molecular weight:
9.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A656D9N9|A0A656D9N9_9BACT FMN-binding domain-containing protein OS=Candidatus Kryptobacter tengchongensis OX=1643429 GN=JGI24_01440 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84LGFILLLFLFSQVLKK17 pKa = 10.37AQSDD21 pKa = 4.0TSSVNLLPQRR31 pKa = 11.84LSLIEE36 pKa = 3.75KK37 pKa = 7.41TLWGEE42 pKa = 3.73RR43 pKa = 11.84GFFRR47 pKa = 11.84IVGIAPLTPEE57 pKa = 4.18ARR59 pKa = 11.84EE60 pKa = 3.85KK61 pKa = 10.41EE62 pKa = 4.09IKK64 pKa = 9.66FRR66 pKa = 11.84RR67 pKa = 11.84TMLSLHH73 pKa = 5.47QLGGFITLGLMTATVYY89 pKa = 10.44YY90 pKa = 8.74GQRR93 pKa = 11.84VFDD96 pKa = 4.4GEE98 pKa = 4.23YY99 pKa = 11.0KK100 pKa = 10.72LINRR104 pKa = 11.84HH105 pKa = 5.51RR106 pKa = 11.84NLVKK110 pKa = 10.06STIASYY116 pKa = 11.56YY117 pKa = 8.9LTASLALFSPPPLVRR132 pKa = 11.84HH133 pKa = 5.7EE134 pKa = 5.01KK135 pKa = 8.12EE136 pKa = 3.97TSSISIHH143 pKa = 6.77KK144 pKa = 9.87ALAWVHH150 pKa = 4.95FAGMISTPLLGLSVKK165 pKa = 10.35KK166 pKa = 10.91SSDD169 pKa = 3.57PIKK172 pKa = 10.19ARR174 pKa = 11.84RR175 pKa = 11.84IHH177 pKa = 6.9RR178 pKa = 11.84ISGYY182 pKa = 8.08ITLASLTSAMIVITFFRR199 pKa = 4.96

Molecular weight:
22.47 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1795

0

1795

558051

39

1608

310.9

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.821 ± 0.055

0.719 ± 0.019

5.084 ± 0.042

7.351 ± 0.079

5.717 ± 0.06

6.736 ± 0.054

1.453 ± 0.023

9.225 ± 0.053

8.147 ± 0.063

9.338 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.025

4.745 ± 0.048

3.789 ± 0.038

2.624 ± 0.034

4.569 ± 0.041

5.91 ± 0.046

4.603 ± 0.041

7.048 ± 0.049

1.041 ± 0.022

4.061 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski