Candidatus Kryptobacter tengchongensis
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1795 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A656D9N8|A0A656D9N8_9BACT Uncharacterized protein (Fragment) OS=Candidatus Kryptobacter tengchongensis OX=1643429 GN=JGI24_01116 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 4.87 VDD4 pKa = 5.82 RR5 pKa = 11.84 NMLWTPEE12 pKa = 4.48 LIRR15 pKa = 11.84 YY16 pKa = 9.4 LEE18 pKa = 4.42 GAPWPATKK26 pKa = 10.25 QEE28 pKa = 4.4 LIEE31 pKa = 3.87 YY32 pKa = 8.79 AKK34 pKa = 10.61 RR35 pKa = 11.84 IGAPPEE41 pKa = 4.1 VIQNLEE47 pKa = 3.98 EE48 pKa = 5.71 LEE50 pKa = 4.95 DD51 pKa = 4.12 SDD53 pKa = 5.09 EE54 pKa = 4.32 EE55 pKa = 4.66 YY56 pKa = 11.39 NGIEE60 pKa = 5.1 DD61 pKa = 3.4 IWPDD65 pKa = 3.56 YY66 pKa = 11.16 SPEE69 pKa = 4.65 DD70 pKa = 3.89 EE71 pKa = 5.58 DD72 pKa = 5.55 DD73 pKa = 3.76 YY74 pKa = 11.78 LYY76 pKa = 11.42 GEE78 pKa = 4.35 EE79 pKa = 5.4 DD80 pKa = 3.71 EE81 pKa = 4.72 YY82 pKa = 12.02
Molecular weight: 9.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.643
IPC_protein 3.579
Toseland 3.401
ProMoST 3.719
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.452
Rodwell 3.414
Grimsley 3.312
Solomon 3.516
Lehninger 3.478
Nozaki 3.668
DTASelect 3.795
Thurlkill 3.439
EMBOSS 3.465
Sillero 3.694
Patrickios 0.769
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.651
Protein with the highest isoelectric point:
>tr|A0A656D9N9|A0A656D9N9_9BACT FMN-binding domain-containing protein OS=Candidatus Kryptobacter tengchongensis OX=1643429 GN=JGI24_01440 PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 LGFILLLFLFSQVLKK17 pKa = 10.37 AQSDD21 pKa = 4.0 TSSVNLLPQRR31 pKa = 11.84 LSLIEE36 pKa = 3.75 KK37 pKa = 7.41 TLWGEE42 pKa = 3.73 RR43 pKa = 11.84 GFFRR47 pKa = 11.84 IVGIAPLTPEE57 pKa = 4.18 ARR59 pKa = 11.84 EE60 pKa = 3.85 KK61 pKa = 10.41 EE62 pKa = 4.09 IKK64 pKa = 9.66 FRR66 pKa = 11.84 RR67 pKa = 11.84 TMLSLHH73 pKa = 5.47 QLGGFITLGLMTATVYY89 pKa = 10.44 YY90 pKa = 8.74 GQRR93 pKa = 11.84 VFDD96 pKa = 4.4 GEE98 pKa = 4.23 YY99 pKa = 11.0 KK100 pKa = 10.72 LINRR104 pKa = 11.84 HH105 pKa = 5.51 RR106 pKa = 11.84 NLVKK110 pKa = 10.06 STIASYY116 pKa = 11.56 YY117 pKa = 8.9 LTASLALFSPPPLVRR132 pKa = 11.84 HH133 pKa = 5.7 EE134 pKa = 5.01 KK135 pKa = 8.12 EE136 pKa = 3.97 TSSISIHH143 pKa = 6.77 KK144 pKa = 9.87 ALAWVHH150 pKa = 4.95 FAGMISTPLLGLSVKK165 pKa = 10.35 KK166 pKa = 10.91 SSDD169 pKa = 3.57 PIKK172 pKa = 10.19 ARR174 pKa = 11.84 RR175 pKa = 11.84 IHH177 pKa = 6.9 RR178 pKa = 11.84 ISGYY182 pKa = 8.08 ITLASLTSAMIVITFFRR199 pKa = 4.96
Molecular weight: 22.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.789
ProMoST 10.613
Dawson 10.877
Bjellqvist 10.584
Wikipedia 11.082
Rodwell 11.184
Grimsley 10.935
Solomon 10.979
Lehninger 10.95
Nozaki 10.745
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.804
Patrickios 10.906
IPC_peptide 10.994
IPC2_peptide 9.341
IPC2.peptide.svr19 8.557
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1795
0
1795
558051
39
1608
310.9
35.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.821 ± 0.055
0.719 ± 0.019
5.084 ± 0.042
7.351 ± 0.079
5.717 ± 0.06
6.736 ± 0.054
1.453 ± 0.023
9.225 ± 0.053
8.147 ± 0.063
9.338 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.003 ± 0.025
4.745 ± 0.048
3.789 ± 0.038
2.624 ± 0.034
4.569 ± 0.041
5.91 ± 0.046
4.603 ± 0.041
7.048 ± 0.049
1.041 ± 0.022
4.061 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here