Bacillus phage 049ML003

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PIB6|A0A5P8PIB6_9CAUD Uncharacterized protein OS=Bacillus phage 049ML003 OX=2601661 GN=35 PE=4 SV=1
MM1 pKa = 7.52SKK3 pKa = 10.59LYY5 pKa = 10.97NYY7 pKa = 10.08FLYY10 pKa = 10.39SWLDD14 pKa = 3.36GTGDD18 pKa = 3.72EE19 pKa = 4.33EE20 pKa = 4.55LLRR23 pKa = 11.84IAVLKK28 pKa = 10.88GDD30 pKa = 4.38LTQEE34 pKa = 4.07EE35 pKa = 4.47ADD37 pKa = 4.75KK38 pKa = 11.23IMSYY42 pKa = 10.31PWGIYY47 pKa = 9.5AA48 pKa = 5.46

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PK46|A0A5P8PK46_9CAUD Uncharacterized protein OS=Bacillus phage 049ML003 OX=2601661 GN=47 PE=4 SV=1
MM1 pKa = 7.61SEE3 pKa = 4.15KK4 pKa = 10.58NRR6 pKa = 11.84FNKK9 pKa = 9.5PVAFNKK15 pKa = 10.44KK16 pKa = 9.98NVDD19 pKa = 3.3DD20 pKa = 4.08QLILKK25 pKa = 9.45HH26 pKa = 4.99VSRR29 pKa = 11.84RR30 pKa = 11.84NFSGYY35 pKa = 8.23VKK37 pKa = 10.63KK38 pKa = 10.94LILADD43 pKa = 3.19IEE45 pKa = 4.5SRR47 pKa = 11.84KK48 pKa = 9.61GLKK51 pKa = 9.34PVRR54 pKa = 11.84EE55 pKa = 4.13AEE57 pKa = 4.35KK58 pKa = 9.99VQKK61 pKa = 9.1TASDD65 pKa = 3.08RR66 pKa = 11.84MEE68 pKa = 3.98EE69 pKa = 4.14MKK71 pKa = 10.64RR72 pKa = 11.84RR73 pKa = 11.84AKK75 pKa = 10.24PSRR78 pKa = 11.84RR79 pKa = 11.84PPGGIFTPTPQQYY92 pKa = 10.37NRR94 pKa = 3.91

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

14537

33

1093

179.5

20.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.299 ± 0.532

0.66 ± 0.103

6.054 ± 0.365

8.385 ± 0.549

3.962 ± 0.169

7.244 ± 0.28

1.706 ± 0.177

6.7 ± 0.237

8.317 ± 0.42

7.305 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.013 ± 0.162

5.366 ± 0.333

3.316 ± 0.182

3.481 ± 0.235

4.808 ± 0.225

5.317 ± 0.289

5.655 ± 0.335

6.26 ± 0.23

1.396 ± 0.151

3.756 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski