Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium; Chaetomium thermophilum; Chaetomium thermophilum var. thermophilum

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0SBF1|G0SBF1_CHATD Beta-xylanase OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) OX=759272 GN=CTHT_0050050 PE=3 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84IWTSIACLSGMVSLATAAVVPRR24 pKa = 11.84VDD26 pKa = 4.75EE27 pKa = 4.43IALSSCTTSEE37 pKa = 3.91EE38 pKa = 4.52SVTVVTITVHH48 pKa = 5.83PQTATTITVTAGVSSPSSLSSALPFPFPGDD78 pKa = 3.27SSLGSEE84 pKa = 4.33STTCTDD90 pKa = 3.63EE91 pKa = 5.76DD92 pKa = 3.88SGSTDD97 pKa = 3.05VSIITVTPSLSWSFTFSTDD116 pKa = 3.26TTSSSADD123 pKa = 3.07AVTAGGPIQTVTVYY137 pKa = 10.48VPAPTPVTVTVGPPFSPSSASTVTSDD163 pKa = 3.48TEE165 pKa = 4.37CPVTITYY172 pKa = 7.78TLSPGPVFPSGFTGTLTLGGSKK194 pKa = 10.1PSSTPEE200 pKa = 3.52VSSAFDD206 pKa = 3.65PFSTFPEE213 pKa = 4.39SSTFGTPSSPILGPVTSSDD232 pKa = 3.4ADD234 pKa = 3.65SSDD237 pKa = 3.34VSVDD241 pKa = 3.17VVTYY245 pKa = 7.37TIPAQGTSSAQIGTVTVTSGAAQSTVSDD273 pKa = 3.83SVFTLTLSVFEE284 pKa = 4.93PSSPTYY290 pKa = 10.06NIPISSQPGTVTVTVTSAPDD310 pKa = 3.6DD311 pKa = 4.85DD312 pKa = 6.31GNDD315 pKa = 5.22DD316 pKa = 5.76DD317 pKa = 6.29DD318 pKa = 5.26DD319 pKa = 3.66WWTVWTNSWEE329 pKa = 4.39SISTITAIDD338 pKa = 3.72DD339 pKa = 3.7HH340 pKa = 8.2SSTVSSQDD348 pKa = 3.27ALSIWTSDD356 pKa = 2.91EE357 pKa = 3.72GATVVTVTEE366 pKa = 4.26IEE368 pKa = 4.35EE369 pKa = 4.2LTATPSSPGKK379 pKa = 9.23PWPTGYY385 pKa = 10.4DD386 pKa = 3.02SSGASVVVVTYY397 pKa = 7.17TIPGGSSTYY406 pKa = 9.56TVTYY410 pKa = 7.59TIPPLSTPSEE420 pKa = 4.21STYY423 pKa = 10.69TPFWSASPSSISSVAAVSSSAPDD446 pKa = 3.68TIVTVTVWEE455 pKa = 4.54TTDD458 pKa = 3.49SLLFPTGDD466 pKa = 3.36TVYY469 pKa = 10.78GGPSSNLPDD478 pKa = 5.26DD479 pKa = 4.63GVLTLTVTVTDD490 pKa = 4.79DD491 pKa = 3.57QPVWATDD498 pKa = 3.15VTTQTGYY505 pKa = 10.55GHH507 pKa = 6.79HH508 pKa = 5.77TTAANASGALPQPYY522 pKa = 10.15GSNTSSRR529 pKa = 11.84SSFDD533 pKa = 2.82PWLTTASGEE542 pKa = 4.32PYY544 pKa = 10.36GGPDD548 pKa = 3.41EE549 pKa = 4.47STVAVSEE556 pKa = 4.46VTFTQTVPLGPDD568 pKa = 2.87SSTVIVVTTEE578 pKa = 3.6VTVDD582 pKa = 3.24VTPTSSSSPTPSPSVIITPVVGTFTLSGAYY612 pKa = 7.56GTTIVLTYY620 pKa = 7.74TTEE623 pKa = 4.11LTITTALPPEE633 pKa = 4.51SSDD636 pKa = 3.74LSTGLVDD643 pKa = 3.89PTSLSPSFSGNFSSPSSSTSCSTSDD668 pKa = 3.45VLGLEE673 pKa = 4.35TSTFSQEE680 pKa = 3.99NPVPTEE686 pKa = 3.82VSVWPPPGPSSTSCSTTSSTSVLPLFTSASSSTTSCSNNSTTSSHH731 pKa = 6.55RR732 pKa = 11.84LAHH735 pKa = 5.74HH736 pKa = 6.83TSHH739 pKa = 7.05ASLGGSSTLGGYY751 pKa = 7.41VFPSSDD757 pKa = 3.03GAYY760 pKa = 9.79GGEE763 pKa = 3.82WSSDD767 pKa = 3.53SEE769 pKa = 4.39FSTVAPLPNSPSSTLTPVISSALPSINSNTILSTPEE805 pKa = 3.42VTLTDD810 pKa = 4.01GPTIPTWSFSSVNLPTISFVSSVSAITGSAEE841 pKa = 3.56ASAAPTAPASSLSTPIPVISTVPTLSSSVAPDD873 pKa = 3.46LTTSHH878 pKa = 7.03EE879 pKa = 4.54SPEE882 pKa = 4.14QTSSSISSSSGVSSSVLSHH901 pKa = 6.63LSSGTEE907 pKa = 3.84LSPSSTISHH916 pKa = 6.06TTTTSISGSPPPPFVPTSWVNSSAPSTLPTEE947 pKa = 4.5VSSTLNGTISPPATLTSSVPAEE969 pKa = 4.04LSSEE973 pKa = 3.73ISTTLNGTIPPVPLTISIVSVPTNVSSTLNGTITPTIPLTSNVVSTFTPSVLPTIPPMPLSIHH1036 pKa = 5.32QPKK1039 pKa = 10.59SSVFSLMTISRR1050 pKa = 11.84EE1051 pKa = 3.85VSPDD1055 pKa = 3.4YY1056 pKa = 10.59VAKK1059 pKa = 10.75CGAEE1063 pKa = 3.96EE1064 pKa = 4.13DD1065 pKa = 3.84RR1066 pKa = 11.84GSVVLTFDD1074 pKa = 5.87DD1075 pKa = 4.39IPPLSVDD1082 pKa = 3.05KK1083 pKa = 10.95SANGEE1088 pKa = 3.96IGPQPVPFPYY1098 pKa = 10.15HH1099 pKa = 6.77RR1100 pKa = 11.84FFFSPDD1106 pKa = 3.03LRR1108 pKa = 11.84VVSSSSVKK1116 pKa = 10.33YY1117 pKa = 10.45KK1118 pKa = 10.4PISGGQLLQHH1128 pKa = 5.91NHH1130 pKa = 4.93SHH1132 pKa = 6.74HH1133 pKa = 6.75SVAQLGLAWLRR1144 pKa = 11.84SNDD1147 pKa = 3.09CFRR1150 pKa = 11.84FNFRR1154 pKa = 11.84GISLGCDD1161 pKa = 2.86SVDD1164 pKa = 3.47APCVFTVTALNWDD1177 pKa = 3.86GVDD1180 pKa = 4.8DD1181 pKa = 4.44AVQGATTLEE1190 pKa = 4.33IPACPAATDD1199 pKa = 3.72CEE1201 pKa = 4.38LRR1203 pKa = 11.84RR1204 pKa = 11.84QILDD1208 pKa = 4.62PITASTFTNLTAVNITLSSPAQAQAWWADD1237 pKa = 4.0DD1238 pKa = 3.96IQISWADD1245 pKa = 3.63TGCNAAACRR1254 pKa = 11.84AKK1256 pKa = 10.92VPDD1259 pKa = 3.2RR1260 pKa = 11.84TMTNNSPKK1268 pKa = 10.29SFPGKK1273 pKa = 8.34PRR1275 pKa = 11.84RR1276 pKa = 11.84YY1277 pKa = 8.34MRR1279 pKa = 11.84WGSRR1283 pKa = 11.84QLHH1286 pKa = 5.67AA1287 pKa = 5.43

Molecular weight: 132.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0S2Y1|G0S2Y1_CHATD Uncharacterized protein OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) OX=759272 GN=CTHT_0018900 PE=4 SV=1
MM1 pKa = 7.36QDD3 pKa = 3.09EE4 pKa = 4.27EE5 pKa = 4.81LLRR8 pKa = 11.84KK9 pKa = 9.56PSQGKK14 pKa = 8.13LTSLLTDD21 pKa = 4.0WVHH24 pKa = 5.65GWQATALLLHH34 pKa = 7.28AIGICPMATPSVWYY48 pKa = 10.09SPHH51 pKa = 6.68GSLNTEE57 pKa = 3.49PMLRR61 pKa = 11.84ARR63 pKa = 11.84GIDD66 pKa = 3.36PHH68 pKa = 7.36NPSITNTAAAAARR81 pKa = 11.84IAAHH85 pKa = 5.94TALFTAAKK93 pKa = 8.83LHH95 pKa = 6.58HH96 pKa = 6.29GMPFAGAAARR106 pKa = 11.84SAIPQPIGSPRR117 pKa = 11.84TQVLKK122 pKa = 11.06LLLCRR127 pKa = 11.84SSMSGRR133 pKa = 11.84NPRR136 pKa = 11.84IRR138 pKa = 11.84SPYY141 pKa = 9.01FLSPTHH147 pKa = 5.54VV148 pKa = 3.13

Molecular weight: 15.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7184

1

7185

3924665

22

8081

546.2

60.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.039 ± 0.029

1.086 ± 0.01

5.489 ± 0.02

6.696 ± 0.047

3.365 ± 0.018

6.853 ± 0.029

2.3 ± 0.012

4.512 ± 0.018

5.007 ± 0.028

8.664 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.011

3.494 ± 0.016

6.867 ± 0.038

4.194 ± 0.029

6.505 ± 0.028

7.993 ± 0.034

5.901 ± 0.02

6.082 ± 0.025

1.368 ± 0.011

2.563 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski