Mycobacterium virus Heldan
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1DAA7|G1DAA7_9CAUD DNA primase OS=Mycobacterium virus Heldan OX=1032892 GN=58 PE=4 SV=1
MM1 pKa = 7.63 TLPKK5 pKa = 10.36 YY6 pKa = 8.63 LTSHH10 pKa = 6.85 SEE12 pKa = 3.96 VDD14 pKa = 2.83 WDD16 pKa = 3.8 AVHH19 pKa = 7.46 DD20 pKa = 4.09 YY21 pKa = 10.92 VYY23 pKa = 10.98 EE24 pKa = 5.18 GDD26 pKa = 4.83 DD27 pKa = 4.06 DD28 pKa = 4.96 NEE30 pKa = 4.05
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.872
IPC_protein 3.745
Toseland 3.554
ProMoST 3.884
Dawson 3.757
Bjellqvist 4.037
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|G1DAC0|G1DAC0_9CAUD Cas4 family exonuclease OS=Mycobacterium virus Heldan OX=1032892 GN=71 PE=4 SV=1
MM1 pKa = 6.65 ITEE4 pKa = 4.13 PVKK7 pKa = 10.82 VNGRR11 pKa = 11.84 ALEE14 pKa = 4.45 PGTEE18 pKa = 4.0 VSVKK22 pKa = 10.48 GEE24 pKa = 3.63 QGRR27 pKa = 11.84 FRR29 pKa = 11.84 FVKK32 pKa = 9.56 ATRR35 pKa = 11.84 TSKK38 pKa = 11.02 GLTVLDD44 pKa = 4.36 FVGGTPGRR52 pKa = 11.84 EE53 pKa = 4.34 AFRR56 pKa = 11.84 SFYY59 pKa = 9.61 PEE61 pKa = 3.59 RR62 pKa = 11.84 VEE64 pKa = 4.3 TVHH67 pKa = 7.15 RR68 pKa = 11.84 IAKK71 pKa = 8.07 TPQNTHH77 pKa = 6.42 PKK79 pKa = 8.99 VKK81 pKa = 10.12 RR82 pKa = 11.84 PKK84 pKa = 9.91 RR85 pKa = 3.58
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 9.794
IPC_protein 10.745
Toseland 11.169
ProMoST 11.418
Dawson 11.213
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.403
Grimsley 11.228
Solomon 11.462
Lehninger 11.418
Nozaki 11.155
DTASelect 10.979
Thurlkill 11.155
EMBOSS 11.608
Sillero 11.155
Patrickios 11.155
IPC_peptide 11.477
IPC2_peptide 9.955
IPC2.peptide.svr19 8.951
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
89
0
89
15897
25
1012
178.6
19.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.511 ± 0.418
0.969 ± 0.128
6.523 ± 0.266
6.756 ± 0.373
3.397 ± 0.211
8.404 ± 0.554
2.101 ± 0.186
4.705 ± 0.162
4.164 ± 0.199
8.002 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.153
3.372 ± 0.212
5.75 ± 0.405
3.711 ± 0.297
6.938 ± 0.36
5.309 ± 0.207
5.636 ± 0.236
7.354 ± 0.204
1.893 ± 0.148
2.975 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here