Oceanicella actignis
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3013 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7TKP1|A0A1M7TKP1_9RHOB Ureidoglycolate lyase OS=Oceanicella actignis OX=1189325 GN=SAMN05216200_107124 PE=4 SV=1
MM1 pKa = 7.47 APKK4 pKa = 10.49 AFALAALAALAAAPAGAGEE23 pKa = 4.4 MFAGSYY29 pKa = 10.79 DD30 pKa = 3.68 GDD32 pKa = 3.46 WLVRR36 pKa = 11.84 VRR38 pKa = 11.84 GIGVIPNEE46 pKa = 4.31 SLTADD51 pKa = 5.37 GIPGSDD57 pKa = 3.35 ANITTSVVPEE67 pKa = 3.85 LDD69 pKa = 2.56 ITYY72 pKa = 9.74 FFTPNIAAEE81 pKa = 4.99 LILGTTPHH89 pKa = 6.84 DD90 pKa = 3.9 VSGRR94 pKa = 11.84 GALAGADD101 pKa = 3.36 IGSVWLLPPTLTLQYY116 pKa = 10.67 HH117 pKa = 5.17 VTQLGEE123 pKa = 4.05 WTGSEE128 pKa = 4.05 SLAKK132 pKa = 10.02 VKK134 pKa = 10.18 PYY136 pKa = 10.42 FGAGVNYY143 pKa = 9.56 TIFYY147 pKa = 10.76 NEE149 pKa = 4.93 DD150 pKa = 3.0 AGQFNSIDD158 pKa = 3.51 YY159 pKa = 11.05 DD160 pKa = 3.85 NAFGAALQAGVDD172 pKa = 3.83 MEE174 pKa = 5.08 LGDD177 pKa = 4.69 GFYY180 pKa = 11.29 LNADD184 pKa = 3.29 VKK186 pKa = 11.02 YY187 pKa = 10.76 IFLEE191 pKa = 4.27 TDD193 pKa = 2.68 WSINDD198 pKa = 3.86 GAITGSVDD206 pKa = 3.2 INPLIVGLGLGYY218 pKa = 10.67 RR219 pKa = 11.84 FF220 pKa = 4.15
Molecular weight: 23.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.88
Protein with the highest isoelectric point:
>tr|A0A1M7SQ62|A0A1M7SQ62_9RHOB Uncharacterized protein OS=Oceanicella actignis OX=1189325 GN=SAMN05216200_10396 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.48 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.03 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.398
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.135
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.099
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3013
0
3013
998641
29
2959
331.4
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
16.458 ± 0.098
0.839 ± 0.013
5.599 ± 0.037
6.292 ± 0.043
3.317 ± 0.024
9.007 ± 0.048
1.852 ± 0.021
4.135 ± 0.032
2.259 ± 0.035
10.326 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.485 ± 0.021
1.834 ± 0.021
6.136 ± 0.042
2.475 ± 0.021
9.083 ± 0.054
4.016 ± 0.025
4.038 ± 0.028
6.655 ± 0.033
1.44 ± 0.021
1.754 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here