Amino acid dipepetide frequency for Paraliobacillus sp. PM-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.757AlaAla: 4.757 ± 0.098
0.559AlaCys: 0.559 ± 0.024
3.303AlaAsp: 3.303 ± 0.067
4.245AlaGlu: 4.245 ± 0.088
3.125AlaPhe: 3.125 ± 0.062
4.509AlaGly: 4.509 ± 0.088
1.221AlaHis: 1.221 ± 0.038
6.413AlaIle: 6.413 ± 0.103
4.596AlaLys: 4.596 ± 0.087
6.878AlaLeu: 6.878 ± 0.123
1.956AlaMet: 1.956 ± 0.049
3.077AlaAsn: 3.077 ± 0.069
1.825AlaPro: 1.825 ± 0.049
2.186AlaGln: 2.186 ± 0.056
2.361AlaArg: 2.361 ± 0.057
4.091AlaSer: 4.091 ± 0.072
3.804AlaThr: 3.804 ± 0.069
4.548AlaVal: 4.548 ± 0.08
0.591AlaTrp: 0.591 ± 0.028
2.451AlaTyr: 2.451 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.355CysAla: 0.355 ± 0.021
0.072CysCys: 0.072 ± 0.009
0.346CysAsp: 0.346 ± 0.019
0.368CysGlu: 0.368 ± 0.022
0.304CysPhe: 0.304 ± 0.02
0.506CysGly: 0.506 ± 0.026
0.19CysHis: 0.19 ± 0.016
0.467CysIle: 0.467 ± 0.025
0.36CysLys: 0.36 ± 0.023
0.583CysLeu: 0.583 ± 0.024
0.167CysMet: 0.167 ± 0.015
0.292CysAsn: 0.292 ± 0.017
0.294CysPro: 0.294 ± 0.02
0.23CysGln: 0.23 ± 0.021
0.226CysArg: 0.226 ± 0.017
0.409CysSer: 0.409 ± 0.021
0.3CysThr: 0.3 ± 0.019
0.347CysVal: 0.347 ± 0.021
0.039CysTrp: 0.039 ± 0.006
0.239CysTyr: 0.239 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.457AspAla: 3.457 ± 0.074
0.335AspCys: 0.335 ± 0.02
2.886AspAsp: 2.886 ± 0.073
4.347AspGlu: 4.347 ± 0.078
2.484AspPhe: 2.484 ± 0.051
3.527AspGly: 3.527 ± 0.074
1.324AspHis: 1.324 ± 0.051
4.528AspIle: 4.528 ± 0.08
3.809AspLys: 3.809 ± 0.07
5.142AspLeu: 5.142 ± 0.074
1.44AspMet: 1.44 ± 0.044
2.577AspAsn: 2.577 ± 0.055
2.096AspPro: 2.096 ± 0.05
2.897AspGln: 2.897 ± 0.07
2.182AspArg: 2.182 ± 0.055
2.72AspSer: 2.72 ± 0.07
2.999AspThr: 2.999 ± 0.069
4.065AspVal: 4.065 ± 0.081
0.692AspTrp: 0.692 ± 0.035
2.397AspTyr: 2.397 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
5.418GluAla: 5.418 ± 0.106
0.267GluCys: 0.267 ± 0.016
4.141GluAsp: 4.141 ± 0.083
6.715GluGlu: 6.715 ± 0.123
2.001GluPhe: 2.001 ± 0.046
3.921GluGly: 3.921 ± 0.076
1.401GluHis: 1.401 ± 0.043
5.573GluIle: 5.573 ± 0.081
6.476GluLys: 6.476 ± 0.092
6.68GluLeu: 6.68 ± 0.112
2.305GluMet: 2.305 ± 0.06
3.969GluAsn: 3.969 ± 0.071
1.909GluPro: 1.909 ± 0.06
3.95GluGln: 3.95 ± 0.083
3.081GluArg: 3.081 ± 0.072
3.678GluSer: 3.678 ± 0.07
4.036GluThr: 4.036 ± 0.074
5.144GluVal: 5.144 ± 0.092
0.795GluTrp: 0.795 ± 0.035
1.976GluTyr: 1.976 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.705PheAla: 2.705 ± 0.066
0.276PheCys: 0.276 ± 0.017
2.599PheAsp: 2.599 ± 0.054
2.64PheGlu: 2.64 ± 0.063
2.3PhePhe: 2.3 ± 0.066
2.948PheGly: 2.948 ± 0.075
0.968PheHis: 0.968 ± 0.036
4.129PheIle: 4.129 ± 0.088
2.392PheLys: 2.392 ± 0.053
4.225PheLeu: 4.225 ± 0.087
1.072PheMet: 1.072 ± 0.036
2.091PheAsn: 2.091 ± 0.058
1.527PhePro: 1.527 ± 0.044
1.625PheGln: 1.625 ± 0.036
1.413PheArg: 1.413 ± 0.036
3.268PheSer: 3.268 ± 0.069
2.534PheThr: 2.534 ± 0.068
3.202PheVal: 3.202 ± 0.069
0.417PheTrp: 0.417 ± 0.029
1.732PheTyr: 1.732 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
4.236GlyAla: 4.236 ± 0.094
0.507GlyCys: 0.507 ± 0.025
3.377GlyAsp: 3.377 ± 0.072
4.365GlyGlu: 4.365 ± 0.078
3.082GlyPhe: 3.082 ± 0.071
4.14GlyGly: 4.14 ± 0.091
1.233GlyHis: 1.233 ± 0.043
5.751GlyIle: 5.751 ± 0.104
4.503GlyLys: 4.503 ± 0.08
5.954GlyLeu: 5.954 ± 0.109
1.931GlyMet: 1.931 ± 0.052
2.852GlyAsn: 2.852 ± 0.075
1.517GlyPro: 1.517 ± 0.044
1.936GlyGln: 1.936 ± 0.046
2.186GlyArg: 2.186 ± 0.055
3.844GlySer: 3.844 ± 0.079
3.724GlyThr: 3.724 ± 0.073
4.591GlyVal: 4.591 ± 0.079
0.727GlyTrp: 0.727 ± 0.032
2.952GlyTyr: 2.952 ± 0.066
0.0GlyXaa: 0.0 ± 0.0
His
1.541HisAla: 1.541 ± 0.044
0.191HisCys: 0.191 ± 0.014
1.084HisAsp: 1.084 ± 0.041
1.198HisGlu: 1.198 ± 0.041
1.029HisPhe: 1.029 ± 0.031
1.362HisGly: 1.362 ± 0.042
0.734HisHis: 0.734 ± 0.037
1.665HisIle: 1.665 ± 0.048
1.08HisLys: 1.08 ± 0.04
2.116HisLeu: 2.116 ± 0.056
0.534HisMet: 0.534 ± 0.026
0.855HisAsn: 0.855 ± 0.029
1.109HisPro: 1.109 ± 0.035
0.985HisGln: 0.985 ± 0.039
0.809HisArg: 0.809 ± 0.038
1.117HisSer: 1.117 ± 0.041
1.202HisThr: 1.202 ± 0.04
1.56HisVal: 1.56 ± 0.046
0.232HisTrp: 0.232 ± 0.017
0.974HisTyr: 0.974 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.133IleAla: 6.133 ± 0.097
0.591IleCys: 0.591 ± 0.026
5.16IleAsp: 5.16 ± 0.085
5.943IleGlu: 5.943 ± 0.089
3.463IlePhe: 3.463 ± 0.084
6.088IleGly: 6.088 ± 0.105
1.801IleHis: 1.801 ± 0.051
6.662IleIle: 6.662 ± 0.121
5.219IleLys: 5.219 ± 0.087
7.1IleLeu: 7.1 ± 0.114
1.842IleMet: 1.842 ± 0.047
3.979IleAsn: 3.979 ± 0.077
3.372IlePro: 3.372 ± 0.067
3.225IleGln: 3.225 ± 0.07
3.076IleArg: 3.076 ± 0.067
5.206IleSer: 5.206 ± 0.09
5.084IleThr: 5.084 ± 0.073
5.647IleVal: 5.647 ± 0.1
0.666IleTrp: 0.666 ± 0.027
2.724IleTyr: 2.724 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.439LysAla: 4.439 ± 0.075
0.278LysCys: 0.278 ± 0.02
4.057LysAsp: 4.057 ± 0.066
6.836LysGlu: 6.836 ± 0.113
1.807LysPhe: 1.807 ± 0.051
4.151LysGly: 4.151 ± 0.076
1.571LysHis: 1.571 ± 0.049
4.756LysIle: 4.756 ± 0.083
6.234LysLys: 6.234 ± 0.112
5.612LysLeu: 5.612 ± 0.091
2.096LysMet: 2.096 ± 0.045
3.634LysAsn: 3.634 ± 0.064
2.06LysPro: 2.06 ± 0.056
4.338LysGln: 4.338 ± 0.09
3.156LysArg: 3.156 ± 0.073
3.304LysSer: 3.304 ± 0.074
3.647LysThr: 3.647 ± 0.074
4.39LysVal: 4.39 ± 0.082
0.807LysTrp: 0.807 ± 0.031
2.273LysTyr: 2.273 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.752LeuAla: 6.752 ± 0.102
0.516LeuCys: 0.516 ± 0.025
5.226LeuAsp: 5.226 ± 0.083
6.623LeuGlu: 6.623 ± 0.1
4.796LeuPhe: 4.796 ± 0.107
5.676LeuGly: 5.676 ± 0.098
1.973LeuHis: 1.973 ± 0.054
7.426LeuIle: 7.426 ± 0.119
6.02LeuLys: 6.02 ± 0.096
9.735LeuLeu: 9.735 ± 0.165
2.371LeuMet: 2.371 ± 0.06
4.277LeuAsn: 4.277 ± 0.08
3.778LeuPro: 3.778 ± 0.073
3.874LeuGln: 3.874 ± 0.067
3.303LeuArg: 3.303 ± 0.07
6.485LeuSer: 6.485 ± 0.099
5.711LeuThr: 5.711 ± 0.085
5.858LeuVal: 5.858 ± 0.09
0.738LeuTrp: 0.738 ± 0.031
3.172LeuTyr: 3.172 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
1.768MetAla: 1.768 ± 0.048
0.148MetCys: 0.148 ± 0.014
1.619MetAsp: 1.619 ± 0.042
2.03MetGlu: 2.03 ± 0.048
1.054MetPhe: 1.054 ± 0.036
1.575MetGly: 1.575 ± 0.043
0.577MetHis: 0.577 ± 0.024
2.263MetIle: 2.263 ± 0.059
2.313MetLys: 2.313 ± 0.05
2.502MetLeu: 2.502 ± 0.055
0.831MetMet: 0.831 ± 0.034
1.591MetAsn: 1.591 ± 0.038
0.978MetPro: 0.978 ± 0.036
1.166MetGln: 1.166 ± 0.036
1.092MetArg: 1.092 ± 0.038
1.477MetSer: 1.477 ± 0.047
1.467MetThr: 1.467 ± 0.044
1.768MetVal: 1.768 ± 0.046
0.198MetTrp: 0.198 ± 0.017
0.762MetTyr: 0.762 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
2.885AsnAla: 2.885 ± 0.068
0.282AsnCys: 0.282 ± 0.018
2.904AsnAsp: 2.904 ± 0.067
3.963AsnGlu: 3.963 ± 0.08
1.608AsnPhe: 1.608 ± 0.04
3.438AsnGly: 3.438 ± 0.074
1.249AsnHis: 1.249 ± 0.042
3.814AsnIle: 3.814 ± 0.066
3.625AsnLys: 3.625 ± 0.066
3.767AsnLeu: 3.767 ± 0.07
1.249AsnMet: 1.249 ± 0.041
2.651AsnAsn: 2.651 ± 0.077
2.064AsnPro: 2.064 ± 0.054
2.907AsnGln: 2.907 ± 0.062
2.2AsnArg: 2.2 ± 0.05
2.187AsnSer: 2.187 ± 0.059
2.587AsnThr: 2.587 ± 0.059
3.188AsnVal: 3.188 ± 0.066
0.655AsnTrp: 0.655 ± 0.032
2.035AsnTyr: 2.035 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
1.917ProAla: 1.917 ± 0.052
0.187ProCys: 0.187 ± 0.015
1.978ProAsp: 1.978 ± 0.049
2.706ProGlu: 2.706 ± 0.075
1.888ProPhe: 1.888 ± 0.053
2.014ProGly: 2.014 ± 0.054
0.732ProHis: 0.732 ± 0.031
3.172ProIle: 3.172 ± 0.06
2.064ProLys: 2.064 ± 0.052
3.058ProLeu: 3.058 ± 0.063
0.867ProMet: 0.867 ± 0.039
1.978ProAsn: 1.978 ± 0.054
0.87ProPro: 0.87 ± 0.042
0.981ProGln: 0.981 ± 0.035
0.91ProArg: 0.91 ± 0.03
2.285ProSer: 2.285 ± 0.054
2.183ProThr: 2.183 ± 0.049
2.414ProVal: 2.414 ± 0.051
0.357ProTrp: 0.357 ± 0.02
1.463ProTyr: 1.463 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
3.44GlnAla: 3.44 ± 0.068
0.184GlnCys: 0.184 ± 0.014
1.998GlnAsp: 1.998 ± 0.056
3.197GlnGlu: 3.197 ± 0.069
1.728GlnPhe: 1.728 ± 0.037
2.214GlnGly: 2.214 ± 0.054
0.911GlnHis: 0.911 ± 0.037
3.069GlnIle: 3.069 ± 0.069
2.892GlnLys: 2.892 ± 0.068
5.002GlnLeu: 5.002 ± 0.101
1.324GlnMet: 1.324 ± 0.045
1.738GlnAsn: 1.738 ± 0.048
1.361GlnPro: 1.361 ± 0.042
2.411GlnGln: 2.411 ± 0.076
1.596GlnArg: 1.596 ± 0.043
2.703GlnSer: 2.703 ± 0.059
2.562GlnThr: 2.562 ± 0.058
2.768GlnVal: 2.768 ± 0.061
0.485GlnTrp: 0.485 ± 0.028
1.409GlnTyr: 1.409 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.173ArgAla: 2.173 ± 0.049
0.183ArgCys: 0.183 ± 0.016
1.915ArgAsp: 1.915 ± 0.047
2.732ArgGlu: 2.732 ± 0.061
1.752ArgPhe: 1.752 ± 0.045
2.05ArgGly: 2.05 ± 0.049
0.698ArgHis: 0.698 ± 0.027
3.087ArgIle: 3.087 ± 0.066
3.086ArgLys: 3.086 ± 0.072
3.696ArgLeu: 3.696 ± 0.072
1.176ArgMet: 1.176 ± 0.039
1.908ArgAsn: 1.908 ± 0.048
1.166ArgPro: 1.166 ± 0.035
1.527ArgGln: 1.527 ± 0.041
1.621ArgArg: 1.621 ± 0.056
2.064ArgSer: 2.064 ± 0.052
2.009ArgThr: 2.009 ± 0.047
2.409ArgVal: 2.409 ± 0.054
0.394ArgTrp: 0.394 ± 0.024
1.592ArgTyr: 1.592 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
3.19SerAla: 3.19 ± 0.074
0.349SerCys: 0.349 ± 0.018
3.301SerAsp: 3.301 ± 0.068
3.827SerGlu: 3.827 ± 0.087
3.333SerPhe: 3.333 ± 0.074
3.969SerGly: 3.969 ± 0.074
1.15SerHis: 1.15 ± 0.039
5.507SerIle: 5.507 ± 0.088
3.946SerLys: 3.946 ± 0.076
5.732SerLeu: 5.732 ± 0.089
1.659SerMet: 1.659 ± 0.048
3.233SerAsn: 3.233 ± 0.071
1.859SerPro: 1.859 ± 0.051
1.961SerGln: 1.961 ± 0.053
1.868SerArg: 1.868 ± 0.051
3.698SerSer: 3.698 ± 0.075
3.311SerThr: 3.311 ± 0.063
4.087SerVal: 4.087 ± 0.07
0.664SerTrp: 0.664 ± 0.032
2.453SerTyr: 2.453 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.834ThrAla: 3.834 ± 0.092
0.376ThrCys: 0.376 ± 0.024
3.199ThrAsp: 3.199 ± 0.066
3.82ThrGlu: 3.82 ± 0.078
2.848ThrPhe: 2.848 ± 0.058
3.955ThrGly: 3.955 ± 0.076
1.145ThrHis: 1.145 ± 0.035
5.445ThrIle: 5.445 ± 0.079
3.585ThrLys: 3.585 ± 0.061
5.363ThrLeu: 5.363 ± 0.076
1.364ThrMet: 1.364 ± 0.041
3.043ThrAsn: 3.043 ± 0.065
2.181ThrPro: 2.181 ± 0.053
1.695ThrGln: 1.695 ± 0.044
1.742ThrArg: 1.742 ± 0.042
3.42ThrSer: 3.42 ± 0.079
3.529ThrThr: 3.529 ± 0.072
4.135ThrVal: 4.135 ± 0.082
0.575ThrTrp: 0.575 ± 0.031
2.288ThrTyr: 2.288 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
4.758ValAla: 4.758 ± 0.084
0.466ValCys: 0.466 ± 0.025
4.153ValAsp: 4.153 ± 0.089
4.782ValGlu: 4.782 ± 0.083
2.982ValPhe: 2.982 ± 0.063
4.362ValGly: 4.362 ± 0.078
1.245ValHis: 1.245 ± 0.039
5.741ValIle: 5.741 ± 0.095
4.456ValLys: 4.456 ± 0.066
6.471ValLeu: 6.471 ± 0.117
1.781ValMet: 1.781 ± 0.047
3.223ValAsn: 3.223 ± 0.065
2.311ValPro: 2.311 ± 0.05
2.358ValGln: 2.358 ± 0.05
2.429ValArg: 2.429 ± 0.062
4.45ValSer: 4.45 ± 0.078
4.214ValThr: 4.214 ± 0.071
4.807ValVal: 4.807 ± 0.087
0.543ValTrp: 0.543 ± 0.027
2.292ValTyr: 2.292 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.5TrpAla: 0.5 ± 0.023
0.063TrpCys: 0.063 ± 0.009
0.525TrpAsp: 0.525 ± 0.031
0.696TrpGlu: 0.696 ± 0.029
0.557TrpPhe: 0.557 ± 0.028
0.593TrpGly: 0.593 ± 0.028
0.199TrpHis: 0.199 ± 0.017
0.831TrpIle: 0.831 ± 0.031
0.771TrpLys: 0.771 ± 0.029
1.121TrpLeu: 1.121 ± 0.04
0.3TrpMet: 0.3 ± 0.02
0.608TrpAsn: 0.608 ± 0.029
0.245TrpPro: 0.245 ± 0.017
0.406TrpGln: 0.406 ± 0.022
0.363TrpArg: 0.363 ± 0.021
0.648TrpSer: 0.648 ± 0.03
0.548TrpThr: 0.548 ± 0.027
0.579TrpVal: 0.579 ± 0.026
0.138TrpTrp: 0.138 ± 0.012
0.394TrpTyr: 0.394 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.237TyrAla: 2.237 ± 0.057
0.265TyrCys: 0.265 ± 0.016
2.134TyrAsp: 2.134 ± 0.051
2.402TyrGlu: 2.402 ± 0.056
1.919TyrPhe: 1.919 ± 0.047
2.412TyrGly: 2.412 ± 0.059
1.056TyrHis: 1.056 ± 0.038
2.686TyrIle: 2.686 ± 0.063
2.019TyrLys: 2.019 ± 0.05
3.57TyrLeu: 3.57 ± 0.064
0.913TyrMet: 0.913 ± 0.033
1.683TyrAsn: 1.683 ± 0.054
1.503TyrPro: 1.503 ± 0.049
2.334TyrGln: 2.334 ± 0.058
1.622TyrArg: 1.622 ± 0.044
2.041TyrSer: 2.041 ± 0.05
2.091TyrThr: 2.091 ± 0.054
2.316TyrVal: 2.316 ± 0.053
0.372TyrTrp: 0.372 ± 0.025
1.669TyrTyr: 1.669 ± 0.048
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2774 proteins (837727 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski