Tomato leaf curl Yemen betasatellite
Average proteome isoelectric point is 5.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3QBA1|I3QBA1_9VIRU Beta C1 OS=Tomato leaf curl Yemen betasatellite OX=1176156 PE=4 SV=1
MM1 pKa = 7.43 TINYY5 pKa = 8.02 RR6 pKa = 11.84 NSKK9 pKa = 9.73 GVEE12 pKa = 4.12 FKK14 pKa = 10.84 IEE16 pKa = 3.66 VHH18 pKa = 6.87 LITEE22 pKa = 4.63 TVRR25 pKa = 11.84 CIIRR29 pKa = 11.84 VATTRR34 pKa = 11.84 EE35 pKa = 3.94 PNMMNRR41 pKa = 11.84 RR42 pKa = 11.84 FIIPYY47 pKa = 9.95 SYY49 pKa = 11.1 DD50 pKa = 3.35 QIIAPFDD57 pKa = 3.97 FNGTEE62 pKa = 4.11 EE63 pKa = 4.37 EE64 pKa = 4.48 IKK66 pKa = 9.5 TALINRR72 pKa = 11.84 YY73 pKa = 9.32 RR74 pKa = 11.84 DD75 pKa = 3.19 TGYY78 pKa = 10.76 SAIRR82 pKa = 11.84 EE83 pKa = 3.97 EE84 pKa = 5.12 DD85 pKa = 2.96 IVEE88 pKa = 4.06 IVDD91 pKa = 3.47 MVIAEE96 pKa = 4.13 NTGLHH101 pKa = 6.14 GLQIIEE107 pKa = 4.55 PYY109 pKa = 9.81 QFRR112 pKa = 11.84 QNFGVV117 pKa = 3.25
Molecular weight: 13.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.095
IPC2_protein 5.232
IPC_protein 5.08
Toseland 5.016
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.169
Wikipedia 4.876
Rodwell 4.965
Grimsley 4.94
Solomon 5.041
Lehninger 4.991
Nozaki 5.156
DTASelect 5.245
Thurlkill 5.003
EMBOSS 4.927
Sillero 5.245
Patrickios 3.999
IPC_peptide 5.054
IPC2_peptide 5.245
IPC2.peptide.svr19 5.234
Protein with the highest isoelectric point:
>tr|I3QBA1|I3QBA1_9VIRU Beta C1 OS=Tomato leaf curl Yemen betasatellite OX=1176156 PE=4 SV=1
MM1 pKa = 7.43 TINYY5 pKa = 8.02 RR6 pKa = 11.84 NSKK9 pKa = 9.73 GVEE12 pKa = 4.12 FKK14 pKa = 10.84 IEE16 pKa = 3.66 VHH18 pKa = 6.87 LITEE22 pKa = 4.63 TVRR25 pKa = 11.84 CIIRR29 pKa = 11.84 VATTRR34 pKa = 11.84 EE35 pKa = 3.94 PNMMNRR41 pKa = 11.84 RR42 pKa = 11.84 FIIPYY47 pKa = 9.95 SYY49 pKa = 11.1 DD50 pKa = 3.35 QIIAPFDD57 pKa = 3.97 FNGTEE62 pKa = 4.11 EE63 pKa = 4.37 EE64 pKa = 4.48 IKK66 pKa = 9.5 TALINRR72 pKa = 11.84 YY73 pKa = 9.32 RR74 pKa = 11.84 DD75 pKa = 3.19 TGYY78 pKa = 10.76 SAIRR82 pKa = 11.84 EE83 pKa = 3.97 EE84 pKa = 5.12 DD85 pKa = 2.96 IVEE88 pKa = 4.06 IVDD91 pKa = 3.47 MVIAEE96 pKa = 4.13 NTGLHH101 pKa = 6.14 GLQIIEE107 pKa = 4.55 PYY109 pKa = 9.81 QFRR112 pKa = 11.84 QNFGVV117 pKa = 3.25
Molecular weight: 13.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.095
IPC2_protein 5.232
IPC_protein 5.08
Toseland 5.016
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.169
Wikipedia 4.876
Rodwell 4.965
Grimsley 4.94
Solomon 5.041
Lehninger 4.991
Nozaki 5.156
DTASelect 5.245
Thurlkill 5.003
EMBOSS 4.927
Sillero 5.245
Patrickios 3.999
IPC_peptide 5.054
IPC2_peptide 5.245
IPC2.peptide.svr19 5.234
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
117
117
117
117.0
13.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.274 ± 0.0
0.855 ± 0.0
4.274 ± 0.0
10.256 ± 0.0
5.128 ± 0.0
5.128 ± 0.0
1.709 ± 0.0
14.53 ± 0.0
2.564 ± 0.0
3.419 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.419 ± 0.0
6.838 ± 0.0
3.419 ± 0.0
3.419 ± 0.0
8.547 ± 0.0
2.564 ± 0.0
7.692 ± 0.0
6.838 ± 0.0
0.0 ± 0.0
5.128 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here