Microbacterium phage Pherbot

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kojivirus; unclassified Kojivirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E392|A0A4D6E392_9CAUD Membrane protein OS=Microbacterium phage Pherbot OX=2562196 GN=25 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 4.23NTRR5 pKa = 11.84LKK7 pKa = 11.08LSGTTWSEE15 pKa = 3.51LLGIVIPTVITVPSGEE31 pKa = 3.87QFRR34 pKa = 11.84WDD36 pKa = 3.61EE37 pKa = 4.43SVGSYY42 pKa = 10.71CSDD45 pKa = 3.04VTGAGLYY52 pKa = 9.77IEE54 pKa = 5.26PSDD57 pKa = 3.76NEE59 pKa = 4.3GNITPDD65 pKa = 2.63SYY67 pKa = 12.07YY68 pKa = 10.55EE69 pKa = 4.01LPEE72 pKa = 3.98PQGDD76 pKa = 3.64INGG79 pKa = 3.71

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E363|A0A4D6E363_9CAUD DNA polymerase I OS=Microbacterium phage Pherbot OX=2562196 GN=37 PE=3 SV=1
MM1 pKa = 7.24NSSMKK6 pKa = 10.69AKK8 pKa = 10.08IAAITAAAEE17 pKa = 4.13AKK19 pKa = 10.55GIVAGGRR26 pKa = 11.84VRR28 pKa = 11.84LTGTEE33 pKa = 3.55RR34 pKa = 11.84TGTVKK39 pKa = 10.29RR40 pKa = 11.84VEE42 pKa = 4.17YY43 pKa = 10.93NDD45 pKa = 3.31IRR47 pKa = 11.84VIWDD51 pKa = 3.15EE52 pKa = 4.34GSRR55 pKa = 11.84QHH57 pKa = 6.92PSITATWTGLHH68 pKa = 6.33RR69 pKa = 11.84LEE71 pKa = 4.98PVTT74 pKa = 4.71

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12795

46

807

224.5

24.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.152 ± 0.435

0.649 ± 0.102

6.487 ± 0.277

6.018 ± 0.376

2.962 ± 0.199

8.245 ± 0.4

1.876 ± 0.175

4.674 ± 0.433

4.642 ± 0.371

8.449 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.11

3.189 ± 0.202

4.783 ± 0.228

4.22 ± 0.193

5.846 ± 0.386

5.862 ± 0.228

6.909 ± 0.319

7.706 ± 0.317

2.079 ± 0.235

2.915 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski