Friedmanniomyces endolithicus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17901 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U0TTZ6|A0A4U0TTZ6_9PEZI Uncharacterized protein OS=Friedmanniomyces endolithicus OX=329885 GN=B0A54_17344 PE=4 SV=1
MM1 pKa = 7.43 SVATLVRR8 pKa = 11.84 LLCTAQLVLAASTGVGDD25 pKa = 4.1 VVASAIGLTKK35 pKa = 10.7 SLTATSASVQTTPSITPTPALKK57 pKa = 10.85 YY58 pKa = 10.44 NSFGNSSSDD67 pKa = 2.96 ACYY70 pKa = 10.06 TSWLGYY76 pKa = 9.09 WSVSRR81 pKa = 11.84 SALALSLSLYY91 pKa = 10.14 NSSILGTTTWTSKK104 pKa = 10.55 ISVPAQAQSTNTQYY118 pKa = 10.12 DD119 pKa = 3.82 TLDD122 pKa = 3.36 EE123 pKa = 4.41 TVYY126 pKa = 11.55 SMDD129 pKa = 3.27 GAFTISTQTIDD140 pKa = 3.36 TTEE143 pKa = 3.98 EE144 pKa = 4.55 VYY146 pKa = 9.17 VTYY149 pKa = 10.62 QSEE152 pKa = 4.15 NASTYY157 pKa = 7.59 TQIQTFTEE165 pKa = 3.98 VTYY168 pKa = 10.22 FKK170 pKa = 11.1 SNVSVPPTPTYY181 pKa = 10.72 DD182 pKa = 3.41 PPKK185 pKa = 10.42 ALTAVSSAAQVTVPLSSSATAAATPGSSPSQPATEE220 pKa = 4.3 TSQASTGEE228 pKa = 4.06 DD229 pKa = 3.42 TLPSSSSQASSPIAQSSQTSSSQDD253 pKa = 3.18 PGGVIVSVLGASDD266 pKa = 3.6 SSSAQGSQSPTGTSNGAKK284 pKa = 9.92 SQLQYY289 pKa = 11.43 SSDD292 pKa = 3.69 STSSQDD298 pKa = 3.06 AGRR301 pKa = 11.84 VTVSTPGASDD311 pKa = 3.3 SGSVRR316 pKa = 11.84 GSQTPTGTSNGAQSQLQYY334 pKa = 11.29 SSDD337 pKa = 3.74 STSSQDD343 pKa = 3.31 PGRR346 pKa = 11.84 VTVSTPGASDD356 pKa = 3.56 SNSVQGGQSPTGGAQSQPQDD376 pKa = 3.4 SSDD379 pKa = 3.72 PNSTQSIGIVLASIFGGGTGSSGVEE404 pKa = 3.75 SSLVPTEE411 pKa = 4.24 NGGDD415 pKa = 3.68 PGNSAIAAGQTAVSTLAIGTASGIQIGAVIASGEE449 pKa = 4.34 SIEE452 pKa = 4.24 TLATGGPAVPQGGQQLTAEE471 pKa = 4.49 LTNAVTVIGTGADD484 pKa = 3.14 ATFVATAPTDD494 pKa = 3.76 PPLVTIGSQIFTLSSLGSTGGVLVDD519 pKa = 4.03 QGTTLTTGSDD529 pKa = 3.29 ASVVTVDD536 pKa = 3.55 GQTVRR541 pKa = 11.84 AGPSGEE547 pKa = 4.31 VIVGSSILTLPLSTTFIATASTDD570 pKa = 3.58 QPVVTIGSQVLTLSSLSGGTGQLLADD596 pKa = 3.85 QEE598 pKa = 4.86 TTVTIGPDD606 pKa = 3.48 GSVVTVDD613 pKa = 3.47 GQTVHH618 pKa = 7.16 AGPSGEE624 pKa = 4.84 LIVGSSTLNLPSGSSASDD642 pKa = 3.31 ASPAITNAKK651 pKa = 9.67 SSEE654 pKa = 3.98 SSAPSGTTPITSGSSALKK672 pKa = 10.29 KK673 pKa = 10.23 QATTEE678 pKa = 4.08 ALCLAIVFACVQALLL693 pKa = 4.11
Molecular weight: 68.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.554
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.897
Patrickios 1.214
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A4U0UMV1|A0A4U0UMV1_9PEZI FolB domain-containing protein OS=Friedmanniomyces endolithicus OX=329885 GN=B0A54_12012 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 NDD4 pKa = 2.91 AGLRR8 pKa = 11.84 GRR10 pKa = 11.84 VRR12 pKa = 11.84 VRR14 pKa = 11.84 VRR16 pKa = 11.84 VRR18 pKa = 11.84 VRR20 pKa = 11.84 VRR22 pKa = 11.84 VRR24 pKa = 11.84 VRR26 pKa = 11.84 VRR28 pKa = 11.84 VRR30 pKa = 11.84 VRR32 pKa = 11.84 VRR34 pKa = 11.84 VRR36 pKa = 11.84 VRR38 pKa = 11.84 VRR40 pKa = 11.84 VRR42 pKa = 11.84 VRR44 pKa = 11.84 VRR46 pKa = 11.84 VRR48 pKa = 11.84 VRR50 pKa = 11.84 VRR52 pKa = 11.84 VRR54 pKa = 11.84 VRR56 pKa = 2.89
Molecular weight: 7.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.296
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17901
0
17901
8776224
51
5763
490.3
53.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.625 ± 0.018
1.087 ± 0.007
5.667 ± 0.013
6.393 ± 0.018
3.338 ± 0.01
7.578 ± 0.018
2.437 ± 0.008
4.179 ± 0.011
4.551 ± 0.016
8.703 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.283 ± 0.008
3.252 ± 0.01
6.095 ± 0.019
4.166 ± 0.013
6.421 ± 0.018
7.804 ± 0.021
6.117 ± 0.015
6.277 ± 0.013
1.361 ± 0.006
2.666 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here