Friedmanniomyces endolithicus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Teratosphaeriaceae; Friedmanniomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4U0TTZ6|A0A4U0TTZ6_9PEZI Uncharacterized protein OS=Friedmanniomyces endolithicus OX=329885 GN=B0A54_17344 PE=4 SV=1
MM1 pKa = 7.43SVATLVRR8 pKa = 11.84LLCTAQLVLAASTGVGDD25 pKa = 4.1VVASAIGLTKK35 pKa = 10.7SLTATSASVQTTPSITPTPALKK57 pKa = 10.85YY58 pKa = 10.44NSFGNSSSDD67 pKa = 2.96ACYY70 pKa = 10.06TSWLGYY76 pKa = 9.09WSVSRR81 pKa = 11.84SALALSLSLYY91 pKa = 10.14NSSILGTTTWTSKK104 pKa = 10.55ISVPAQAQSTNTQYY118 pKa = 10.12DD119 pKa = 3.82TLDD122 pKa = 3.36EE123 pKa = 4.41TVYY126 pKa = 11.55SMDD129 pKa = 3.27GAFTISTQTIDD140 pKa = 3.36TTEE143 pKa = 3.98EE144 pKa = 4.55VYY146 pKa = 9.17VTYY149 pKa = 10.62QSEE152 pKa = 4.15NASTYY157 pKa = 7.59TQIQTFTEE165 pKa = 3.98VTYY168 pKa = 10.22FKK170 pKa = 11.1SNVSVPPTPTYY181 pKa = 10.72DD182 pKa = 3.41PPKK185 pKa = 10.42ALTAVSSAAQVTVPLSSSATAAATPGSSPSQPATEE220 pKa = 4.3TSQASTGEE228 pKa = 4.06DD229 pKa = 3.42TLPSSSSQASSPIAQSSQTSSSQDD253 pKa = 3.18PGGVIVSVLGASDD266 pKa = 3.6SSSAQGSQSPTGTSNGAKK284 pKa = 9.92SQLQYY289 pKa = 11.43SSDD292 pKa = 3.69STSSQDD298 pKa = 3.06AGRR301 pKa = 11.84VTVSTPGASDD311 pKa = 3.3SGSVRR316 pKa = 11.84GSQTPTGTSNGAQSQLQYY334 pKa = 11.29SSDD337 pKa = 3.74STSSQDD343 pKa = 3.31PGRR346 pKa = 11.84VTVSTPGASDD356 pKa = 3.56SNSVQGGQSPTGGAQSQPQDD376 pKa = 3.4SSDD379 pKa = 3.72PNSTQSIGIVLASIFGGGTGSSGVEE404 pKa = 3.75SSLVPTEE411 pKa = 4.24NGGDD415 pKa = 3.68PGNSAIAAGQTAVSTLAIGTASGIQIGAVIASGEE449 pKa = 4.34SIEE452 pKa = 4.24TLATGGPAVPQGGQQLTAEE471 pKa = 4.49LTNAVTVIGTGADD484 pKa = 3.14ATFVATAPTDD494 pKa = 3.76PPLVTIGSQIFTLSSLGSTGGVLVDD519 pKa = 4.03QGTTLTTGSDD529 pKa = 3.29ASVVTVDD536 pKa = 3.55GQTVRR541 pKa = 11.84AGPSGEE547 pKa = 4.31VIVGSSILTLPLSTTFIATASTDD570 pKa = 3.58QPVVTIGSQVLTLSSLSGGTGQLLADD596 pKa = 3.85QEE598 pKa = 4.86TTVTIGPDD606 pKa = 3.48GSVVTVDD613 pKa = 3.47GQTVHH618 pKa = 7.16AGPSGEE624 pKa = 4.84LIVGSSTLNLPSGSSASDD642 pKa = 3.31ASPAITNAKK651 pKa = 9.67SSEE654 pKa = 3.98SSAPSGTTPITSGSSALKK672 pKa = 10.29KK673 pKa = 10.23QATTEE678 pKa = 4.08ALCLAIVFACVQALLL693 pKa = 4.11

Molecular weight:
68.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4U0UMV1|A0A4U0UMV1_9PEZI FolB domain-containing protein OS=Friedmanniomyces endolithicus OX=329885 GN=B0A54_12012 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84NDD4 pKa = 2.91AGLRR8 pKa = 11.84GRR10 pKa = 11.84VRR12 pKa = 11.84VRR14 pKa = 11.84VRR16 pKa = 11.84VRR18 pKa = 11.84VRR20 pKa = 11.84VRR22 pKa = 11.84VRR24 pKa = 11.84VRR26 pKa = 11.84VRR28 pKa = 11.84VRR30 pKa = 11.84VRR32 pKa = 11.84VRR34 pKa = 11.84VRR36 pKa = 11.84VRR38 pKa = 11.84VRR40 pKa = 11.84VRR42 pKa = 11.84VRR44 pKa = 11.84VRR46 pKa = 11.84VRR48 pKa = 11.84VRR50 pKa = 11.84VRR52 pKa = 11.84VRR54 pKa = 11.84VRR56 pKa = 2.89

Molecular weight:
7.02 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17901

0

17901

8776224

51

5763

490.3

53.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.625 ± 0.018

1.087 ± 0.007

5.667 ± 0.013

6.393 ± 0.018

3.338 ± 0.01

7.578 ± 0.018

2.437 ± 0.008

4.179 ± 0.011

4.551 ± 0.016

8.703 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.008

3.252 ± 0.01

6.095 ± 0.019

4.166 ± 0.013

6.421 ± 0.018

7.804 ± 0.021

6.117 ± 0.015

6.277 ± 0.013

1.361 ± 0.006

2.666 ± 0.01

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski