Pseudomonas phage H72

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A4MZG5|A0A5A4MZG5_9CAUD Repressor OS=Pseudomonas phage H72 OX=2301637 GN=H72_20 PE=4 SV=1
MM1 pKa = 7.88IITLSPYY8 pKa = 10.19VVLPGSDD15 pKa = 3.34EE16 pKa = 4.27HH17 pKa = 8.94LMLSRR22 pKa = 11.84DD23 pKa = 3.49GDD25 pKa = 3.91VLTVNGQSFDD35 pKa = 3.76FTPLPEE41 pKa = 5.14GSEE44 pKa = 4.28LPAEE48 pKa = 4.83AIGSEE53 pKa = 4.3WFAGPVVRR61 pKa = 11.84RR62 pKa = 11.84EE63 pKa = 3.77GRR65 pKa = 11.84LEE67 pKa = 3.71FSLRR71 pKa = 11.84LPLTDD76 pKa = 3.75GASAAARR83 pKa = 11.84FPEE86 pKa = 4.35PMLIEE91 pKa = 4.44ADD93 pKa = 3.97GPVEE97 pKa = 4.28LPQQ100 pKa = 5.18

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A4N004|A0A5A4N004_9CAUD Uncharacterized protein OS=Pseudomonas phage H72 OX=2301637 GN=H72_18 PE=4 SV=1
MM1 pKa = 7.43SVEE4 pKa = 3.86AYY6 pKa = 9.26IRR8 pKa = 11.84GMAARR13 pKa = 11.84GLSRR17 pKa = 11.84SAAAAALGMHH27 pKa = 6.19WVKK30 pKa = 10.86FMDD33 pKa = 4.19LLEE36 pKa = 4.46RR37 pKa = 11.84MPDD40 pKa = 3.48IEE42 pKa = 3.84WGYY45 pKa = 9.72PYY47 pKa = 10.86KK48 pKa = 10.97SFDD51 pKa = 3.46RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.75AKK57 pKa = 8.96NLKK60 pKa = 9.27GYY62 pKa = 9.66RR63 pKa = 11.84FRR65 pKa = 11.84DD66 pKa = 3.25SEE68 pKa = 3.95GRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84SVAALRR78 pKa = 11.84AVNQARR84 pKa = 11.84RR85 pKa = 11.84HH86 pKa = 5.7EE87 pKa = 4.21YY88 pKa = 9.63TVFGVTDD95 pKa = 3.54SLSNLVKK102 pKa = 10.76RR103 pKa = 11.84FGCVAKK109 pKa = 9.97STVQKK114 pKa = 10.6RR115 pKa = 11.84LAKK118 pKa = 10.33GMSIEE123 pKa = 4.25QALTTPRR130 pKa = 11.84SDD132 pKa = 3.27HH133 pKa = 7.13LSGLKK138 pKa = 9.9RR139 pKa = 11.84KK140 pKa = 9.64PEE142 pKa = 3.72SHH144 pKa = 5.74PWKK147 pKa = 8.72RR148 pKa = 11.84AEE150 pKa = 3.75RR151 pKa = 11.84RR152 pKa = 11.84GVINHH157 pKa = 6.65RR158 pKa = 11.84EE159 pKa = 3.9RR160 pKa = 11.84QLKK163 pKa = 9.83AKK165 pKa = 10.3RR166 pKa = 11.84EE167 pKa = 4.02QGQAAAQQLL176 pKa = 3.72

Molecular weight: 20.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12465

47

1210

226.6

24.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.379 ± 0.657

0.858 ± 0.131

5.808 ± 0.183

6.378 ± 0.374

2.856 ± 0.188

7.597 ± 0.365

1.781 ± 0.179

4.083 ± 0.213

3.891 ± 0.313

10.189 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.139

3.016 ± 0.169

4.862 ± 0.33

5.191 ± 0.366

7.453 ± 0.317

5.792 ± 0.232

5.327 ± 0.242

6.306 ± 0.285

1.749 ± 0.157

2.359 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski